FastQCFastQC Report
Fri 17 Jun 2016
SRR1526149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7011
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.4421623163600057No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC140.19968620738838966No Hit
CTTCTGGGGAAAAAAAAACAAAAAAAAAAAAAAAAAAAAA130.18542290686064755No Hit
CTTCTGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT90.12836970474967907No Hit
CTTCTGGGGCTCGTCTTAATCAGCAACATCTTTTGATACC80.11410640422193695No Hit
CTTCTGGGGTAAAAAAAACAAAAAAAAAAAAAAAAAAAAA80.11410640422193695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCAGA251.8462229E-433.957
TCTGGGT200.002623641433.953
TGGGGTG251.8462229E-433.955
TGGGGGC359.1095717E-733.955
TGGGGCT251.8462229E-433.955
TGGGGCC301.2978717E-533.955
GGGGCCA200.002623641433.956
CTGGGGC1200.032.5354164
TGGGGGG750.031.6866665
CTGGGGG2700.031.4351884
TGGGGGT653.6379788E-1231.338465
TCTGGGG6600.031.1208343
TGGGGCA606.002665E-1131.1208345
CTGGGGA1750.031.044
TTCTGGG7350.030.9476182
CTTCTGG7650.030.6215671
TGGGGGA950.030.3763165
TGGGGAA950.030.3763165
GGGGAAG451.7961611E-730.1777766
TGGGGAG402.5865575E-629.7062515
Produced by FastQC (version 0.11.5)