FastQCFastQC Report
Fri 17 Jun 2016
SRR1526148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276
Sequences flagged as poor quality0
Sequence length40
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC170.7469244288224955No Hit
CTTCTGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT120.5272407732864675No Hit
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.35149384885764495No Hit
CTTCTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT60.26362038664323373No Hit
CTTCTGGGGGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC60.26362038664323373No Hit
CTTCTGGGCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC60.26362038664323373No Hit
CTTCTGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT50.21968365553602814No Hit
CTTCTGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.21968365553602814No Hit
CTTCTGGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT40.17574692442882248No Hit
CTTCTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.17574692442882248No Hit
CTTCTGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT30.13181019332161686No Hit
CTTCTGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.13181019332161686No Hit
CTTCTGGGGCTCTTCTTTTTTTTTTTTTTTTTTTTTTTTT30.13181019332161686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGGC700.033.7111134
TGGGGCC251.5323458E-433.7111135
TGGGGCA251.5323458E-433.7111135
TGGGGAC251.5323458E-433.7111135
CTGGGGA650.033.711114
TGGGGAA309.869302E-633.711115
TCTGGGG2200.031.4126263
CTTCTGG2450.030.9591851
TTCTGGG2300.030.7797092
CTGGGGT304.4757753E-428.0925924
CTGGGGG551.66674E-827.581824
Produced by FastQC (version 0.11.5)