FastQCFastQC Report
Fri 17 Jun 2016
SRR1526147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2330
Sequences flagged as poor quality0
Sequence length40
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT662.832618025751073No Hit
CTTCTGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT492.1030042918454934No Hit
CTTCTGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG381.6309012875536482No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC381.6309012875536482No Hit
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.8154506437768241No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG160.6866952789699571No Hit
CTTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.6008583690987125No Hit
CTTCTGGGGACTAATCCTAGCCCTAGCCCTACACAAATAT130.5579399141630901No Hit
CTTCTGGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT70.30042918454935624No Hit
CTTCTGGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGT60.2575107296137339No Hit
CTTCTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.2575107296137339No Hit
CTTCTGGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.2145922746781116No Hit
CTTCTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC50.2145922746781116No Hit
CTTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA50.2145922746781116No Hit
CTTCTGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT50.2145922746781116No Hit
CTTCTGGGGATGCCATCTACCTTCTTCAACCTCACCATAG50.2145922746781116No Hit
CTTCTGGGGGGGCTGGTGAGCTGGCTCAGTGGGTAAGAGC50.2145922746781116No Hit
CTTCTGGGGTCCCAGATCCTCTTCTGAAGGAGATTGGGCG50.2145922746781116No Hit
CTTCTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGG50.2145922746781116No Hit
CTTCTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT40.17167381974248927No Hit
CTTCTGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTT40.17167381974248927No Hit
CTTCTGGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG40.17167381974248927No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG40.17167381974248927No Hit
CTTCTGGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT40.17167381974248927No Hit
CTTCTGGGGAGGCTTTGGAATGAGAGCTTATGATAAGCCC30.12875536480686695No Hit
CTTCTGGGGATGCCACAACTAGATACATCAACATGATTTA30.12875536480686695No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGC30.12875536480686695No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC30.12875536480686695No Hit
CTTCTGGGGTACCTGGTTGATACTGCCAGTAGCATATGCT30.12875536480686695No Hit
CTTCTGGGGTCACAGCTCCTCTTCTGAGATGAGTTTTTGT30.12875536480686695No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG30.12875536480686695No Hit
CTTCTGGGGGGGCCTGGAGAGATGCCTCAGTGGTTAAGAG30.12875536480686695No Hit
CTTCTGGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGA30.12875536480686695No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC30.12875536480686695No Hit
CTTCTGGGGTAAAAGATAATCTTCTGAGATGAGTTTTTGT30.12875536480686695No Hit
CTTCTGGGGTAGTTTGATTTTCCCTAAACATAAACTCATG30.12875536480686695No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAG30.12875536480686695No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTG30.12875536480686695No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGC30.12875536480686695No Hit
CTTCTGGGGTATTTCAAATATTTAATCAATTAGAGGTAAA30.12875536480686695No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGGAC30.12875536480686695No Hit
CTTCTGGGGAGTGCAGTGCTCTTCTGGGGTCACAGATCCT30.12875536480686695No Hit
CTTCTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC30.12875536480686695No Hit
CTTCTGGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCG30.12875536480686695No Hit
CTTCTGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGC30.12875536480686695No Hit
CTTCTGGGGTCACAGATCCTCTTCTGAGATGAGTTCTTGT30.12875536480686695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTGAA200.001908771235.09090822
GGTGAGA200.00239887633.5652215
CTGGGGG1250.033.565224
CTGGGGC200.00239887633.565224
GCTGGTG200.00239887633.5652212
GGCTGGT200.00239887633.5652211
GTGAGAT200.00239887633.5652216
TGGGGTC404.0872692E-833.565225
CTGGTGA200.00239887633.5652213
TGGGGGC200.00239887633.565225
TGGGGGA200.00239887633.565225
GGGGTCA356.545997E-733.565226
TGGTGAG200.00239887633.5652214
TGGGGGG750.033.5652165
TCTGGGG2250.032.073433
TTCTGGG2450.031.5102042
CTTCTGG2450.031.5102041
CTGGGGT507.014023E-930.2086944
ATCCTCT451.15453076E-729.83574916
GATCCTC451.15453076E-729.83574915
Produced by FastQC (version 0.11.5)