FastQCFastQC Report
Fri 17 Jun 2016
SRR1526146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41452
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT4561.1000675480073339No Hit
GTTGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC4351.049406542506996No Hit
GTTGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC1470.35462703850236416No Hit
GTTGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT940.22676831033484512No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC910.2195310238347969No Hit
GTTGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA800.19299430666795328No Hit
GTTGCCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC760.18334459133455563No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC730.17610730483450737No Hit
GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.15198301650101323No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG610.1471581588343144No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT500.12062144166747081No Hit
GTTGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC490.1182090128341214No Hit
GTTGCCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT490.1182090128341214No Hit
GTTGCCGTCTTCTGCTTGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGATCG460.11097172633407315Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG460.11097172633407315No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGAC203.9606474E-450.44200541
CCAATCC354.7801223E-850.44199841
CGCTCTT150.00820508750.25790847
CCGCTCT150.00820508750.25790846
ACCAACA150.00820508750.25790842
CAACACA309.875221E-750.25790844
TTGACCA204.032926E-450.25790843
ATTGACC204.032926E-450.25790842
AACACAA251.990696E-550.25790845
CCCCGCT150.0082844250.13592511
GCGCATT204.0816911E-450.13592538
CGCATTG150.0082844250.13592539
CGACTGA252.0196061E-550.13592540
GGCGCAT204.106249E-450.0751537
GGGCGCA252.0341911E-550.0751536
GGCTCTC204.1309238E-450.014539
GGTTGCC150.00836432350.0145269
GTCAAAG150.00836432350.0145269
CCCGATC150.00840448849.95405249
GCCCGAT150.00840448849.95405248