##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526146_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	41452
Sequences flagged as poor quality	0
Sequence length	56
%GC	54
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.46955514812313	39.0	38.0	39.0	33.0	40.0
2	37.709712438483066	39.0	38.0	39.0	35.0	40.0
3	36.86507285535077	39.0	38.0	39.0	31.0	40.0
4	37.301843095628676	39.0	38.0	39.0	33.0	40.0
5	37.35706359162405	39.0	38.0	39.0	33.0	40.0
6	37.38159799285921	39.0	38.0	39.0	34.0	40.0
7	36.93592589018624	38.0	37.0	39.0	33.0	40.0
8	36.898629740422656	39.0	37.0	39.0	33.0	40.0
9	36.9602431728264	39.0	38.0	39.0	33.0	40.0
10	36.432862105567885	39.0	36.0	39.0	31.0	40.0
11	35.392381549744286	38.0	35.0	39.0	28.0	40.0
12	34.17046222136447	38.0	33.0	39.0	23.0	40.0
13	33.57244523786548	38.0	32.0	39.0	20.0	40.0
14	34.16520312650777	38.0	33.0	39.0	22.0	40.0
15	33.03864710991026	38.0	31.0	39.0	18.0	40.0
16	32.65548103830937	37.0	30.0	39.0	19.0	40.0
17	31.695479108366303	36.0	28.0	39.0	15.0	40.0
18	31.101949242497348	35.0	27.0	38.0	14.0	40.0
19	32.188989674804596	36.0	29.0	38.0	18.0	40.0
20	32.903454598089354	36.0	30.0	39.0	21.0	40.0
21	33.04624626073531	38.0	31.0	39.0	19.0	40.0
22	32.2605181897134	37.0	29.0	39.0	16.0	40.0
23	32.955008202258036	38.0	31.0	39.0	19.0	40.0
24	32.32685998263051	37.0	30.0	39.0	17.0	40.0
25	32.74022966322494	37.0	30.0	39.0	19.0	40.0
26	32.94041300781627	38.0	32.0	39.0	18.0	40.0
27	33.19755379716298	38.0	32.0	39.0	19.0	40.0
28	33.28958795715526	38.0	33.0	39.0	20.0	40.0
29	32.65454019106436	37.0	31.0	39.0	17.0	40.0
30	32.55169834989868	37.0	31.0	39.0	17.0	40.0
31	33.20064170606967	38.0	33.0	39.0	19.0	40.0
32	33.15473318537103	38.0	33.0	39.0	19.0	40.0
33	32.85149088101901	37.0	32.0	39.0	18.0	40.0
34	32.69386278104796	37.0	32.0	39.0	17.0	40.0
35	32.65750747852938	37.0	32.0	39.0	17.0	40.0
36	32.12341985911416	36.0	31.0	38.0	11.0	40.0
37	31.78659654540191	36.0	31.0	38.0	8.0	40.0
38	31.110730483450737	36.0	30.0	38.0	4.0	40.0
39	30.009215478143396	35.0	27.0	38.0	4.0	39.0
40	27.960629161439737	34.0	19.0	38.0	4.0	39.0
41	26.101394383865674	31.0	15.0	37.0	4.0	39.0
42	26.798272700955323	32.0	19.0	37.0	4.0	39.0
43	27.065063205635433	32.0	19.0	38.0	4.0	39.0
44	26.120524944514138	31.0	15.0	37.0	4.0	39.0
45	26.900197819164333	32.0	20.0	37.0	4.0	39.0
46	24.88186336003088	30.0	13.0	36.0	4.0	39.0
47	23.89139245392261	28.0	9.0	36.0	4.0	39.0
48	24.452065039081347	29.0	11.0	36.0	4.0	39.0
49	23.553121682910355	28.0	6.0	35.0	4.0	39.0
50	24.173743124577825	29.0	4.0	36.0	4.0	39.0
51	24.371152176010806	30.0	4.0	36.0	4.0	39.0
52	24.205273569429703	30.0	4.0	36.0	4.0	39.0
53	24.067572131622118	30.0	4.0	36.0	4.0	39.0
54	23.621610537489143	30.0	4.0	36.0	4.0	39.0
55	23.430160185274534	30.0	4.0	36.0	4.0	39.0
56	22.53099971050854	28.0	4.0	36.0	4.0	38.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	1.7751996662287937
1	2	1.368581275473666
1	3	2.9870322878802824
1	4	1.538825993321467
1	5	1.6102484237778256
1	6	1.8907964419774501
1	7	2.4667598275393274
1	8	2.5823500907590784
1	9	2.416062365624704
1	10	2.58281640367278
1	11	0.2747779959452288
1	12	-6.991943034764013
1	13	-18.36396760168195
1	14	-6.8216252635842345
1	15	0.8984798088555443
1	16	-2.374620484462657
1	17	-5.369252438200771
1	18	-5.052744740249096
1	19	0.18385105588699346
1	20	-1.3231719107065096
1	21	5.726391695147658
1	22	6.77325332112575
1	23	5.792173989940178
1	24	5.929316550124323
1	25	5.581089809248663
1	26	5.471721497160885
1	27	5.326531625161792
1	28	5.647765174888939
1	29	5.802566682769079
1	30	6.223692863407745
1	31	4.93042604846671
1	32	4.804707580852348
1	33	5.265161382968618
1	34	4.341410854764355
1	35	6.009646106347436
1	36	4.5218449189221275
1	37	4.959017348792404
1	38	5.484053119602184
1	39	5.893995744027546
1	40	-9.881663714093957
1	41	-0.34461681276620126
1	42	-1.116483548228807
1	43	-7.702793550462399
1	44	-15.478474297099526
1	45	-2.350405765196296
1	46	-4.485171576386538
1	47	-9.735228694801664
1	48	-4.401740628758279
1	49	-13.191487224103966
1	50	-2.472380968644135
1	51	3.4574495584517138
1	52	2.842109326247929
1	53	3.347108735497436
1	54	3.2779931748880884
1	55	2.9554271095138347
1	56	6.289955263006249
2	1	2.039519887070753
2	2	1.3520181284757342
2	3	3.1947617564793163
2	4	1.6050785813131796
2	5	1.8027950076287595
2	6	2.055047649706921
2	7	2.4136197309209706
2	8	2.5043652736438275
2	9	2.7604377969566514
2	10	2.319876445080652
2	11	3.529436381038394
2	12	-1.7441859609199852
2	13	0.9010427502849225
2	14	4.346766730894714
2	15	4.728017421001848
2	16	3.107091040727134
2	17	6.539650253310619
2	18	4.6014995661691245
2	19	4.862249951677192
2	20	4.526379504062298
2	21	6.509000390799837
2	22	6.957518331477715
2	23	5.952974542045073
2	24	6.097708544603275
2	25	5.378881389648942
2	26	5.234316390190557
2	27	4.6143163042508135
2	28	3.7526651058758276
2	29	4.737694357027191
2	30	5.804093139460196
2	31	4.79378008573379
2	32	4.438247953523149
2	33	4.202359450601467
2	34	3.2717076111480665
2	35	3.689425264387463
2	36	4.191272110088448
2	37	4.783724043064314
2	38	4.711796390823718
2	39	5.737335978672132
2	40	3.6745819725865125
2	41	6.650552269359402
2	42	6.350045092212877
2	43	5.213700583423041
2	44	-5.123056767768954
2	45	5.181685332112192
2	46	4.630080321474054
2	47	1.5539362465371909
2	48	4.525795603125779
2	49	-2.1155728003634557
2	50	5.028654228043234
2	51	7.245579303103199
2	52	7.4777200922934774
2	53	7.8122571136892915
2	54	8.456737136240747
2	55	7.9913139280093475
2	56	9.009071895166361
3	1	2.0805072059113314
3	2	1.5478967516641404
3	3	3.5269628434358395
3	4	1.3476057552262262
3	5	1.965159138063541
3	6	2.3838519975330072
3	7	2.5223153830948846
3	8	2.6230246939336794
3	9	2.459713159275495
3	10	3.3975485465299258
3	11	3.771057757849988
3	12	4.966638314442335
3	13	5.39198477927043
3	14	5.151341006257034
3	15	0.8287873485380821
3	16	6.0556870552198845
3	17	6.708128514180181
3	18	7.840856450227093
3	19	6.91252169080763
3	20	6.009169359134759
3	21	7.122225028480994
3	22	7.230842606840035
3	23	6.422630339146526
3	24	6.95957448663226
3	25	7.031734650518505
3	26	5.340521100335486
3	27	5.412097101352266
3	28	4.630156047904812
3	29	5.139868270070668
3	30	5.757671038010923
3	31	4.42670581037147
3	32	4.100838533233297
3	33	4.683564160746396
3	34	4.477323553177051
3	35	4.608356423807752
3	36	5.293174284001495
3	37	5.93272766625272
3	38	5.738517405316468
3	39	6.977825109106913
3	40	8.369328349398106
3	41	10.834202631678242
3	42	9.785054150183893
3	43	9.060847322553474
3	44	9.733827290202061
3	45	9.461350187184657
3	46	11.038141915676952
3	47	11.444334797261828
3	48	10.921176037908388
3	49	12.20236198224524
3	50	10.76348212659396
3	51	10.708441621943777
3	52	10.198508135771739
3	53	10.383815084703784
3	54	10.245007063776978
3	55	10.618125522212246
3	56	9.992088199514185
4	1	2.5686135491949145
4	2	2.1283538412163807
4	3	3.706766947913451
4	4	2.2410292626889117
4	5	2.339459511197866
4	6	2.520978863204647
4	7	2.6641064278710047
4	8	2.8422411118441246
4	9	2.836578830917283
4	10	2.9644795166791766
4	11	4.797876787700737
4	12	6.1781588368303915
4	13	5.972208659867448
4	14	6.015380185361515
4	15	6.900848915702259
4	16	7.224763547757199
4	17	8.41894940970257
4	18	9.466089659182316
4	19	7.490413481852407
4	20	7.021296224806399
4	21	7.089575774749651
4	22	7.71568402475048
4	23	7.245857951086823
4	24	7.50761553140838
4	25	6.705941740070745
4	26	6.880866249589218
4	27	6.563916131203015
4	28	5.535706421039137
4	29	6.524196628279622
4	30	6.413221411145248
4	31	5.811733795446099
4	32	5.01081987651688
4	33	4.669804832388184
4	34	6.308480269594959
4	35	5.7599422447032715
4	36	5.946625776538809
4	37	6.380955278193017
4	38	6.93394650979408
4	39	7.348627347912885
4	40	3.2564552150697494
4	41	10.428185840633464
4	42	9.596854523318218
4	43	10.498580531508697
4	44	11.317782514082655
4	45	10.064661754348833
4	46	13.439727736572472
4	47	13.428476588306605
4	48	12.981343948356148
4	49	13.447697803140759
4	50	13.831812350474554
4	51	13.968702815973629
4	52	13.606857016368757
4	53	13.56198672649483
4	54	13.435538780194886
4	55	13.114394178928663
4	56	12.778702005484334
5	1	2.09265998368911
5	2	1.6364384183308047
5	3	3.389810065658061
5	4	1.8285085330040047
5	5	1.9634230269524267
5	6	1.8769075530885644
5	7	2.0223153830948846
5	8	2.6716358050447937
5	9	2.751379825942159
5	10	2.76734021319659
5	11	4.008904980072209
5	12	5.718374425553449
5	13	5.461429223714873
5	14	5.3787715618125915
5	15	6.257606792982525
5	16	6.80395094410877
5	17	8.055350736402403
5	18	8.415509228004872
5	19	7.06877169080763
5	20	5.793891581356981
5	21	6.351391695147658
5	22	7.461745384617814
5	23	6.946935894702083
5	24	6.976935597743374
5	25	6.255692983851841
5	26	5.269340544779929
5	27	4.927722101352266
5	28	4.965225492349255
5	29	5.487090492292889
5	30	5.8427404824553655
5	31	5.345108588149248
5	32	5.368199644344411
5	33	4.876272494079732
5	34	4.803712442065937
5	35	5.993773090474416
5	36	6.456368728445938
5	37	7.02647766625272
5	38	7.283656294205354
5	39	7.800741775773577
5	40	8.695717238286992
5	41	11.072049853900463
5	42	10.220818039072778
5	43	10.670222322553474
5	44	11.747716179090947
5	45	11.365864076073542
5	46	13.145780804565838
5	47	12.715168130595163
5	48	13.376037149019503
5	49	14.500973093356354
5	50	13.970079348816181
5	51	12.020941621943777
5	52	11.818299802438403
5	53	10.612981751370452
5	54	10.833548730443646
5	55	11.90979218887891
5	56	10.990352088403075
6	1	2.187898078927205
6	2	1.479692386584773
6	3	3.209254510102504
6	4	1.6816831361786058
6	5	1.7372325507619522
6	6	2.08127263245364
6	7	2.482632843412347
6	8	2.4394929479019325
6	9	2.9398718894342224
6	10	3.2018640227204003
6	11	3.68747640864364
6	12	5.420755377934398
6	13	6.239207001492652
6	14	5.210120768161794
6	15	6.184194094569829
6	16	6.8158557060135365
6	17	7.710112641164308
6	18	8.407572720068366
6	19	7.533057405093345
6	20	5.99428840675381
6	21	6.3454393141952785
6	22	6.662142210014636
6	23	6.188999386765577
6	24	7.278522899330671
6	25	5.993788221947074
6	26	5.916165941605328
6	27	5.151928450558614
6	28	5.48903501615878
6	29	5.897804778007174
6	30	5.826867466582353
6	31	5.089156207196869
6	32	4.804707580852348
6	33	4.296907414714649
6	34	4.008077521431019
6	35	4.850915947617278
6	36	5.744067141144349
6	37	6.149493539268594
6	38	6.753894389443449
6	39	6.719392569424375
6	40	3.9913521589219165
6	41	9.925224457075064
6	42	9.153357721612462
6	43	9.567047719378873
6	44	11.045335226709998
6	45	9.189276774486245
6	46	11.594193502978541
6	47	12.137787178214207
6	48	11.48714826013061
6	49	12.268830236213493
6	50	11.908571412308245
6	51	11.013005114007271
6	52	9.746871231009834
6	53	10.267743656132357
6	54	11.262120159015073
6	55	11.43161758570431
6	56	11.067733040784027
7	1	1.9656758567049835
7	2	1.574930481822868
7	3	3.2886195894675865
7	4	1.8086672631627323
7	5	1.9594547729841736
7	6	2.3987329499139562
7	7	2.006442367221865
7	8	2.9791754875844703
7	9	2.6859036354659693
7	10	2.281229102085483
7	11	4.004936726103956
7	12	5.023929981108999
7	13	5.763016525302177
7	14	5.543454101495129
7	15	5.961971872347608
7	16	6.30791919807703
7	17	7.408525339577004
7	18	7.772652085147726
7	19	6.596549468585408
7	20	5.518097930563329
7	21	6.0438520126079744
7	22	7.471666019538446
7	23	6.649316847083032
7	24	7.1991578199655955
7	25	5.866804094962948
7	26	6.074896100335486
7	27	5.151928450558614
7	28	4.7430032701270335
7	29	5.278757158959554
7	30	5.636391276106163
7	31	4.358997477038134
7	32	3.5824853586301195
7	33	3.5191296369368708
7	34	4.357283870637367
7	35	4.8985349952363215
7	36	5.537717934795147
7	37	5.625684015459068
7	38	5.976116611665677
7	39	7.909868759900565
7	40	6.610399777969537
7	41	10.036335568186178
7	42	10.915262483517228
7	43	9.995619147950297
7	44	10.775493956868726
7	45	10.808324393533866
7	46	12.641812550597585
7	47	13.042549082976112
7	48	12.693497466479819
7	49	13.681528648911904
7	50	12.63873014246698
7	51	13.330465431467587
7	52	12.588141072279676
7	53	12.426473814862511
7	54	12.135136032030946
7	55	12.2252683793551
7	56	11.797891770942758
8	1	2.2367572059113314
8	2	1.6260217516641404
8	3	3.4644628434358395
8	4	1.8163557552262262
8	5	2.043284138063541
8	6	1.7432269975330072
8	7	2.3348153830948846
8	8	2.9667746939336794
8	9	2.944088159275495
8	10	2.7725485465299258
8	11	3.771057757849988
8	12	4.607263314442335
8	13	5.65760977927043
8	14	4.963841006257034
8	15	5.828787348538082
8	16	6.2900620552198845
8	17	7.739378514180181
8	18	8.403356450227093
8	19	6.92814669080763
8	20	5.665419359134759
8	21	6.497225028480994
8	22	7.402717606840035
8	23	6.438255339146526
8	24	6.55332448663226
8	25	6.328609650518505
8	26	5.996771100335486
8	27	5.287097101352266
8	28	5.661406047904812
8	29	6.546118270070668
8	30	6.117046038010923
8	31	5.25483081037147
8	32	5.022713533233297
8	33	4.605439160746396
8	34	4.461698553177051
8	35	4.327106423807752
8	36	4.808799284001495
8	37	4.99522766625272
8	38	4.863517405316468
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115	28	5.667122511319448
115	29	6.853282904217007
115	30	5.910491159962145
115	31	5.00940398110318
115	32	5.049771460062562
115	33	5.823426965624446
115	34	5.576408919030712
115	35	5.595399106734579
115	36	6.984485259611247
115	37	6.935014251618572
115	38	6.867328380926221
115	39	8.015172670082524
115	40	10.25499908110542
115	41	10.22177885119044
115	42	10.459977930671695
115	43	6.82875890791933
115	44	-4.33019710004184
115	45	7.28490201645295
115	46	1.3971358181159772
115	47	-8.511457885665001
115	48	1.630779696444975
115	49	-3.790016066535248
115	50	2.061881516837861
115	51	4.813624548773046
115	52	4.749194111381495
115	53	4.208510206655003
115	54	5.044930844264783
115	55	5.221784058797613
115	56	8.445975394636136
116	1	2.144669439953887
116	2	1.6107291984726544
116	3	3.05322614130818
116	4	1.2369009679921845
116	5	2.026661797638006
116	6	2.169756252852153
116	7	2.72444304266935
116	8	2.674886396061339
116	9	2.8054578401265573
116	10	2.89588631248737
116	11	3.079235949339349
116	12	3.740242037846592
116	13	3.4850698856534095
116	14	4.916301112640014
116	15	-1.5405610557172373
116	16	4.016125885007121
116	17	5.859724258861036
116	18	5.393050599163267
116	19	5.414516371658692
116	20	4.606243827219863
116	21	5.166108007204397
116	22	6.590882500457056
116	23	5.8222314029763105
116	24	6.333909593015239
116	25	5.528742629241908
116	26	5.521704610973785
116	27	4.994211463054391
116	28	4.605887430883534
116	29	5.879229972198328
116	30	5.228083272053478
116	31	6.0327563422863655
116	32	5.395386405573721
116	33	6.383364692661292
116	34	5.6355682340281135
116	35	5.412212806786474
116	36	6.844371092512134
116	37	6.76583936838038
116	38	6.8974269797845515
116	39	9.216521917617552
116	40	9.43714749833428
116	41	8.589189333805901
116	42	7.092567448056233
116	43	1.191498918298155
116	44	3.130103885946742
116	45	7.601975187184657
116	46	1.6451897880173796
116	47	-5.072952436780724
116	48	1.0521600804615794
116	49	-2.644712485839868
116	50	2.8143464882960885
116	51	7.797537366624628
116	52	8.034611859175996
116	53	7.1856767868314435
116	54	7.613690574415276
116	55	6.751104245616503
116	56	9.886037667599293
117	1	1.2871044281335529
117	2	1.0392161961085833
117	3	2.6259211767691752
117	4	1.495175199670669
117	5	1.4078674713968766
117	6	1.6130186641996715
117	7	1.8278709386504417
117	8	2.268858027267015
117	9	2.1541576037199377
117	10	2.197895768752147
117	11	0.12001609118332368
117	12	-6.726070018890997
117	13	-10.677459665174013
117	14	-2.1073395492985227
117	15	-6.5896154292396965
117	16	-6.8349379447801155
117	17	0.7497951808468493
117	18	-2.3622685497729066
117	19	0.6243272463631868
117	20	0.55778047024587
117	21	5.531947250703215
117	22	6.003412051284478
117	23	5.05804700581319
117	24	5.5047133755211455
117	25	5.866804094962948
117	26	5.713784989224372
117	27	5.358277656907823
117	28	4.298558825682591
117	29	5.223201603404004
117	30	5.481629371344258
117	31	4.1367752548159125
117	32	5.812644088788854
117	33	5.848494716301953
117	34	4.56760133095483
117	35	5.632661979363309
117	36	6.5258131728903805
117	37	6.8320332218082775
117	38	6.9364340719831326
117	39	8.481297331329134
117	40	10.54293946050922
117	41	8.072049853900463
117	42	6.38748470573945
117	43	-2.107555455224304
117	44	0.27549395686872913
117	45	7.5464196316290995
117	46	-1.4931080843230475
117	47	-0.9931652027381723
117	48	-0.5961850732027223
117	49	-3.6518046844214282
117	50	0.8173015710384028
117	51	4.71538606638822
117	52	5.76274424688285
117	53	5.251870640259341
117	54	5.75021539711031
117	55	6.173681077767803
117	56	9.032018755069743
118	1	1.1384116176760344
118	2	0.16830116342884338
118	3	2.2484701963770135
118	4	1.0066131081674001
118	5	1.0304164910047149
118	6	1.2110578798859493
118	7	1.8164330301537106
118	8	1.7149364586395635
118	9	1.5920661004519658
118	10	1.9021441347652228
118	11	0.536682757849988
118	12	-6.378030803204723
118	13	-4.688897573670747
118	14	-3.728254581978259
118	15	-10.238308239697211
118	16	-4.629055591838938
118	17	-0.2404008975845251
118	18	-3.5975626674199646
118	19	-1.5047577209570768
118	20	0.005492888546523744
118	21	2.736195616716291
118	22	3.382497018604738
118	23	2.4649097509112288
118	24	3.3070009572204917
118	25	2.951771415224389
118	26	4.457249041511957
118	27	3.8615456307640343
118	28	2.991369283198928
118	29	3.3310447406589
118	30	4.310060743893278
118	31	3.1040955162538246
118	32	3.345323827350942
118	33	2.866468572511099
118	34	2.569235317882935
118	35	3.915341717925397
118	36	5.916336048707379
118	37	5.747065901546836
118	38	5.416826228845881
118	39	6.729663344401029
118	40	9.681828349398106
118	41	7.393945278737064
118	42	7.4348703266544796
118	43	0.14081055784759045
118	44	4.638239054907945
118	45	5.74903401071407
118	46	-1.169578672558341
118	47	1.5803642090265342
118	48	0.029631920261330436
118	49	1.1325090410687686
118	50	2.8287394795351375
118	51	5.873882798414364
118	52	4.445750782830562
118	53	3.0492562611743708
118	54	3.490411475541684
118	55	4.662243169271068
118	56	5.9617573171612435
119	1	1.2123181033472292
119	2	0.4345153414077316
119	3	2.4143827152307082
119	4	0.8135512680467372
119	5	0.9655597790891832
119	6	1.369428920609927
119	7	1.9453923061718044
119	8	1.980396488805475
119	9	1.6456105951729327
119	10	1.853878674735057
119	11	0.3187340399012726
119	12	-9.309403352224333
119	13	-1.9317331694475186
119	14	-4.679989121948093
119	15	-5.920812010436276
119	16	-8.886219996062167
119	17	-0.7245637935121252
119	18	-6.426371113875472
119	19	-0.8478949758590382
119	20	-1.0789716665062663
119	21	2.8033147720707383
119	22	4.648711196583626
119	23	3.6029188006849857
119	24	3.070952691760464
119	25	3.5505647787236363
119	26	5.22874225418164
119	27	5.469388768018931
119	28	5.187447714571476
119	29	5.1847400649424635
119	30	4.988039627754514
119	31	3.794894912935572
119	32	5.090421866566633
119	33	3.889093006900243
119	34	4.2962338095873065
119	35	4.480952577653905
119	36	5.784360181437393
119	37	6.250836640611691
119	38	6.154382789931855
119	39	6.848818698850504
119	40	8.463879631449391
119	41	5.920340452191063
119	42	5.966544534799276
119	43	-1.086187933856781
119	44	-0.6604034790287088
119	45	5.8327444179538865
119	46	2.9556098643949014
119	47	1.192732233159262
119	48	-4.119689346706995
119	49	-0.0877021203188626
119	50	2.2638827676196023
119	51	6.7773518783540325
119	52	5.574710058848662
119	53	5.183494571883269
119	54	5.474574371469284
119	55	7.1629973170840415
119	56	6.9956939687449555
120	1	0.8013686923978156
120	2	0.060987967880357985
120	3	2.2452905461385413
120	4	0.6791091336046051
120	5	0.2975071110365164
120	6	0.8247303759113862
120	7	0.99528835606786
120	8	0.9693084777174619
120	9	0.5610645106268493
120	10	0.9689167897731679
120	11	-1.0443983232310927
120	12	-10.561655604476584
120	13	-7.7127449504592995
120	14	-8.092324534283506
120	15	-1.7780538676781354
120	16	-6.2133163231584945
120	17	0.42096635201802
120	18	-5.997403684908043
120	19	0.8187716908076297
120	20	-0.4857630732976723
120	21	4.24891421767018
120	22	6.2211297690021965
120	23	4.763001960768147
120	24	4.892851513659284
120	25	3.6926299207887787
120	26	4.3181393435787285
120	27	3.8027221013522663
120	28	3.9261864533102155
120	29	4.9844628646652644
120	30	3.695593335308221
120	31	4.611249729290389
120	32	4.351683127827894
120	33	2.7186148364220735
120	34	3.0668505802040755
120	35	4.056836153537478
120	36	4.724762121839333
120	37	4.546747936522994
120	38	5.526524162073226
120	39	6.826642676674478
120	40	5.965612133181889
120	41	0.5052330370836486
120	42	-1.5261789579242162
120	43	-5.045993893662743
120	44	-13.891172709797939
120	45	-3.452078866869396
120	46	-5.5877026789176405
120	47	-2.972894932467902
120	48	-1.6269658539835028
120	49	-13.877029909646652
120	50	-1.728494224757391
120	51	4.034455135457289
120	52	2.73271421685282
120	53	3.966585354974054
120	54	3.2209361178310303
120	55	3.016774170860895
120	56	5.388625361676347
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	180.0
5	43.0
6	170.0
7	97.0
8	295.0
9	409.0
10	211.0
11	215.0
12	222.0
13	134.0
14	173.0
15	175.0
16	210.0
17	243.0
18	286.0
19	367.0
20	457.0
21	628.0
22	685.0
23	922.0
24	1092.0
25	1191.0
26	1386.0
27	1389.0
28	1499.0
29	1669.0
30	1751.0
31	1939.0
32	2204.0
33	2564.0
34	2734.0
35	2871.0
36	3171.0
37	3577.0
38	3431.0
39	2859.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	98.94818102865966	0.6055196371707035	0.18334459133455563	0.26295474283508635
8	97.61410788381743	1.2086268455080575	1.049406542506996	0.12785872816751906
9	93.98340248962656	2.1784232365145226	1.9468300685129787	1.8913442053459424
10	49.13635047766091	24.445141368329633	17.716877352118114	8.701630801891344
11	31.6052301457107	21.57917591431053	18.38270771012255	28.43288622985622
12	28.295377786355303	24.283508636495224	21.69256006947795	25.728553507671524
13	23.559779986490398	17.014860561613432	22.968734922319793	36.456624529576374
14	21.726334073144844	28.73685226285825	27.004728360513365	22.532085303483548
15	33.45529440271384	17.671432032953717	25.67482432759874	23.198449236733705
16	28.932184157794357	28.393809903189204	22.857970594625915	19.816035344390524
17	26.462214072000773	19.81710259627449	31.049126532188016	22.671556799536724
18	25.480073337836533	20.79513654347197	19.42246453729615	34.30232558139535
19	28.24145587951342	22.05541610349488	21.06825642015833	28.634871596833367
20	26.98589099342868	24.425009663703133	29.894665635871664	18.69443370699652
21	26.009022701502982	19.75344382523944	25.753299076017466	28.484234397240115
22	23.123130367654156	21.419955611309465	33.77882852455853	21.678085496477852
23	25.164045160667758	21.921740808646145	31.624529576377498	21.289684454308595
24	25.366689182669113	22.31737913731545	21.685322782977902	30.630608897037536
25	23.950968800521196	22.561108027893734	29.013343628598314	24.474579542986753
26	32.25276979893311	24.084578435395496	20.8984044992638	22.76424726640759
27	22.947653080934906	30.97112227158586	20.99430171914236	25.086922928336875
28	26.942655183228393	31.425056090323515	19.302308750090468	22.329979976357627
29	30.719222775387887	27.427618541253807	21.248006186862582	20.60515249649572
30	29.95534097767049	22.55159927579964	26.136391068195536	21.35666867833434
31	31.961200656307305	26.18473120355178	21.226232989093717	20.627835151047197
32	32.555445616236696	25.310712648116418	22.27477857953037	19.859063156116513
33	31.6286597738912	25.311624311527687	23.821142139337134	19.238573775243985
34	22.340939856894217	23.020208857087603	36.52098240185651	18.11786888416167
35	21.736713633177853	26.819247407980278	33.35427894724122	18.089760011600646
36	27.059192738509076	27.095403630745462	26.05252993433758	19.792873696407877
37	29.46834219429676	26.184146930884484	26.08506524891252	18.262445625906235
38	30.863899030440773	25.474503735583546	24.67661210377427	18.984985130201405
39	28.75401676774022	25.443958539708618	20.759138901640533	25.04288579091063
40	24.307819570823806	19.53432922972118	23.624154542358035	32.533696657096975
41	19.383850447244633	18.01896219747008	21.69684857052329	40.900338784762
42	19.577999854816465	28.32288818447988	24.076269751010233	28.022842209693422
43	19.294936892499635	24.894820832728858	27.012911649499493	28.797330625272018
44	20.94262394342319	25.689650996633485	27.801593644796434	25.56613141514689
45	25.797949511558176	24.231066834316664	27.265693007060644	22.705290647064512
46	20.79684551744158	27.77105810634283	24.81977841211476	26.61231796410083
47	19.561580057712362	24.4259075147312	23.19163898251655	32.82087344503989
48	26.214623780546702	23.184101226697575	25.74374577417174	24.857529218583984
49	20.40915026357789	25.373603520820236	24.556270251970787	29.66097596363109
50	20.338491295938105	26.56189555125725	26.25	26.84961315280464
51	19.65526424092769	27.377926259471757	26.244946377127366	26.721863122473188
52	19.858327490752608	31.448395909387617	27.275584459541136	21.417692140318643
53	18.719450225039928	30.315055897013988	28.51715626966075	22.44833760828534
54	18.556227327690447	23.419588875453446	31.588875453446192	26.435308343409915
55	18.750605092458127	23.264594830090036	31.063026430438573	26.921773647013264
56	19.813215262151896	25.324816723524712	30.1420241465244	24.71994386779899
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	3.0
7	6.0
8	11.0
9	16.0
10	20.5
11	25.0
12	59.5
13	121.0
14	148.0
15	144.5
16	141.0
17	129.0
18	117.0
19	110.0
20	103.0
21	105.5
22	109.5
23	111.0
24	120.5
25	130.0
26	149.0
27	168.0
28	180.0
29	235.5
30	279.0
31	268.0
32	257.0
33	298.5
34	340.0
35	389.5
36	439.0
37	558.0
38	688.0
39	699.0
40	717.0
41	735.0
42	826.5
43	918.0
44	1046.5
45	1175.0
46	1450.5
47	2281.5
48	2837.0
49	3032.5
50	3228.0
51	2821.5
52	2415.0
53	2328.5
54	2309.0
55	2376.0
56	2452.0
57	2528.0
58	2619.5
59	2711.0
60	2845.5
61	2980.0
62	3073.5
63	2879.0
64	2591.0
65	2239.0
66	1887.0
67	1623.5
68	1360.0
69	1144.5
70	929.0
71	801.0
72	533.5
73	394.0
74	301.0
75	208.0
76	187.5
77	167.0
78	122.5
79	64.0
80	50.0
81	35.0
82	20.0
83	12.0
84	4.0
85	2.0
86	0.0
87	1.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.4390620476695938
16	0.07478529383383191
17	0.01929943066679533
18	0.0
19	0.04824857666698832
20	0.14474573000096497
21	0.0024124288333494163
22	0.0
23	0.0
24	0.0
25	0.021711859500144745
26	0.055485863167036575
27	0.08684743800057898
28	0.0024124288333494163
29	0.17851973366785678
30	0.06513557850043424
31	0.01929943066679533
32	0.03618643250024124
33	0.13509601466756732
34	0.20264402200135095
35	0.1809321625012062
36	0.06754800733378366
37	0.17369487600115796
38	0.2243558815014957
39	0.15198301650101323
40	0.8443500916722956
41	1.0204573965068031
42	0.30155360416867705
43	0.22676831033484512
44	0.3932258998359549
45	0.23159316800154395
46	0.27501688700183347
47	0.5138473415034256
48	0.09649715333397664
49	0.2364180256682428
50	0.2219434526681463
51	0.34980218083566533
52	0.21470616616809804
53	0.30396603300202646
54	0.24606774100164047
55	0.3280903213355206
56	0.2919038888352794
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	41452.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.24722570684165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.14763019679808	67.97259480845315
2	6.495602100866924	9.905432789732703
3	1.9110295513510092	4.371321046029141
4	0.8447763082958932	2.5764739940171766
5	0.4714294754160603	1.797259480845315
6	0.2815921027652977	1.2882369970085883
7	0.16452572296399418	0.8781240953391876
8	0.12972220464468773	0.7912766573386085
9	0.06011516800607479	0.4125253305027502
>10	0.46193760678352214	6.204766959374698
>50	0.022147693475922294	1.297886712341986
>100	0.009491868632538125	2.504101129016694
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTTGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	456	1.1000675480073339	No Hit
GTTGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	435	1.049406542506996	No Hit
GTTGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	147	0.35462703850236416	No Hit
GTTGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	94	0.22676831033484512	No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	91	0.2195310238347969	No Hit
GTTGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	80	0.19299430666795328	No Hit
GTTGCCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	76	0.18334459133455563	No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	73	0.17610730483450737	No Hit
GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	63	0.15198301650101323	No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	61	0.1471581588343144	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	50	0.12062144166747081	No Hit
GTTGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	49	0.1182090128341214	No Hit
GTTGCCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	49	0.1182090128341214	No Hit
GTTGCCGTCTTCTGCTTGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGATCG	46	0.11097172633407315	Illumina Single End Adapter 2 (97% over 34bp)
GTTGCCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG	46	0.11097172633407315	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0048248576666988325	0.0	0.0	0.0	0.0
11	0.0048248576666988325	0.0	0.0	0.0	0.0
12	0.007237286500048248	0.0	0.0	0.0	0.0
13	0.009649715333397665	0.0	0.0	0.0	0.0
14	0.07237286500048248	0.0	0.0	0.0	0.0
15	0.07237286500048248	0.0	0.0	0.0	0.0
16	0.08443500916722957	0.0	0.0	0.0	0.0
17	0.08684743800057898	0.0	0.0	0.0	0.0
18	0.09408472450062723	0.0	0.0	0.0	0.0
19	0.09408472450062723	0.0	0.0	0.0	0.0
20	0.09890958216732607	0.0	0.0	0.0	0.0
21	0.10132201100067548	0.0	0.0	0.0	0.0
22	0.1037344398340249	0.0	0.0	0.0	0.0
23	0.10614686866737431	0.0	0.0	0.0	0.0
24	0.10855929750072373	0.0	0.0	0.0	0.0
25	0.12062144166747081	0.0	0.0	0.0	0.0
26	0.13268358583421788	0.0	0.0	0.0	0.0
27	0.14715815883431438	0.0	0.0	0.0	0.0
28	0.15922030300106146	0.0	0.0	0.0	0.0
29	0.15922030300106146	0.0	0.0	0.0	0.0
30	0.15922030300106146	0.0	0.0	0.0	0.0
31	0.16645758950110973	0.0	0.0	0.0024124288333494163	0.0
32	0.16645758950110973	0.0	0.0	0.0024124288333494163	0.0
33	0.16645758950110973	0.0	0.0	0.0024124288333494163	0.0
34	0.16887001833445914	0.0	0.0	0.0024124288333494163	0.0
35	0.17369487600115796	0.0	0.0	0.0024124288333494163	0.0
36	0.17369487600115796	0.0	0.0	0.0024124288333494163	0.0
37	0.1785197336678568	0.0	0.0	0.0024124288333494163	0.0
38	0.18093216250120622	0.0	0.0	0.0024124288333494163	0.0
39	0.18575702016790505	0.0	0.0	0.0024124288333494163	0.0
40	0.18575702016790505	0.0	0.0	0.0024124288333494163	0.0
41	0.18575702016790505	0.0	0.0	0.0024124288333494163	0.0
42	0.18816944900125446	0.0	0.0	0.0024124288333494163	0.0
43	0.2074688796680498	0.0	0.0	0.0024124288333494163	0.0
44	0.21229373733474863	0.0	0.0	0.0024124288333494163	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTGAC	20	3.9606474E-4	50.442005	41
CCAATCC	35	4.7801223E-8	50.441998	41
CGCTCTT	15	0.008205087	50.257908	47
CCGCTCT	15	0.008205087	50.257908	46
ACCAACA	15	0.008205087	50.257908	42
CAACACA	30	9.875221E-7	50.257908	44
TTGACCA	20	4.032926E-4	50.257908	43
ATTGACC	20	4.032926E-4	50.257908	42
AACACAA	25	1.990696E-5	50.257908	45
CCCCGCT	15	0.00828442	50.135925	11
GCGCATT	20	4.0816911E-4	50.135925	38
CGCATTG	15	0.00828442	50.135925	39
CGACTGA	25	2.0196061E-5	50.135925	40
GGCGCAT	20	4.106249E-4	50.07515	37
GGGCGCA	25	2.0341911E-5	50.07515	36
GGCTCTC	20	4.1309238E-4	50.01453	9
GGTTGCC	15	0.008364323	50.014526	9
GTCAAAG	15	0.008364323	50.014526	9
CCCGATC	15	0.008404488	49.954052	49
GCCCGAT	15	0.008404488	49.954052	48
>>END_MODULE