FastQCFastQC Report
Fri 17 Jun 2016
SRR1526145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16678
Sequences flagged as poor quality0
Sequence length40
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT2461.4749970020386136No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT1170.7015229643842188No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT1170.7015229643842188No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT1100.6595515049766159No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT360.21585321981052885No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCCGTCGCCTATGCT340.20386137426549947No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCCTCTGCT310.18587360594795538No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG290.17388176040292602No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC280.16788583763041132No Hit
GTTGCCGGGTACCTGGTTCATCCTGCCCGTAGCCTATGCT280.16788583763041132No Hit
GTTGCCGTCTTCTGCTTGGATCGGAAGAGCTCGTATGCCG260.15589399208538196Illumina Single End Adapter 2 (95% over 23bp)
GTTGCCGGGTACCTGGTTGATCCTGCCCGTAGCATCTGCT260.15589399208538196No Hit
GTTGCCGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC250.14989806931286725No Hit
GTTGCCGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT240.14390214654035255No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCCGTCGCATATGCT240.14390214654035255No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATCTGCT230.13790622376783787No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC220.1319103009953232No Hit
GTTGCCGGGTACCTGGTTCATCCTGCCCGTAGCATATGCT210.1259143782228085No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG200.1199184554502938No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTCGCCTATGCT190.1139225326777791No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA190.1139225326777791No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCC180.10792660990526443No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCCGCAGCCTATGCT180.10792660990526443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGT900.034.12084624
CCCGTCG407.056042E-834.12084626
CCCGTAG503.6743586E-1034.12084626
CTGCCCG1000.034.12084623
GCCCGTA503.6743586E-1034.12084625
CGGGGGC650.034.0180746
TGCCGGA650.034.0180743
CGGGATC650.034.0180746
CCGGGAC1050.034.0180745
TTCATCC455.2405085E-934.0180717
CGGGGTG200.002703877434.018076
CGGGGGG1150.034.018076
TGCCGGC200.002703877434.018073
CGGGGCT351.0037911E-634.018076
CGGGGCC200.002703877434.018076
CGGGGCA200.002703877434.018076
TGCCGCG301.3927765E-534.018073
CGGGGAC351.0037911E-634.018076
CATCCTG407.247945E-834.0180719
GGTCCCT200.002703877434.018078
Produced by FastQC (version 0.11.5)