FastQCFastQC Report
Fri 17 Jun 2016
SRR1526143_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526143_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4377
Sequences flagged as poor quality0
Sequence length40
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT701.5992689056431346No Hit
GTTGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG631.439342015078821No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC320.731094356865433No Hit
GTTGCCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC200.4569339730408956No Hit
GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.411240575736806No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA140.3198537811286269No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG130.29700708247658214No Hit
GTTGCCGGGGTTTGTGACGGGGGGCTGGGGACTCTCTCTT130.29700708247658214No Hit
GTTGCCGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG120.2741603838245374No Hit
GTTGCCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT120.2741603838245374No Hit
GTTGCCGGGACTAATCCTAGCCCTAGCCCTACACAAATAT120.2741603838245374No Hit
GTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.205620287868403No Hit
GTTGCCGGGGCTGTTGCCGGGTCACAGATCCTCTTCTGAG90.205620287868403No Hit
GTTGCCGGGGCTCCTGGGAGGGGCGTTCTCCTGGCTTGAG80.18277358921635825No Hit
GTTGCCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT80.18277358921635825No Hit
GTTGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA80.18277358921635825No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC60.1370801919122687No Hit
GTTGCCGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC60.1370801919122687No Hit
GTTGCCGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAGC60.1370801919122687No Hit
GTTGCCGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG60.1370801919122687No Hit
GTTGCCGTCTTCTGCTTGGATCGGAAGAGCTCGTATGCCG60.1370801919122687Illumina Single End Adapter 2 (95% over 23bp)
GTTGCCGGGGGACTGAGGTGTGGGTGTGTTCTATATTCCA60.1370801919122687No Hit
GTTGCCGGGGCTGTTGCCGGGTCACAGATCCTCTTCTGAA60.1370801919122687No Hit
GTTGCCGGGGTGTTCTTTATTAATATCCTAACACTCCTCG60.1370801919122687No Hit
GTTGCCGGGCTGTTGCCGGGTCACAGATCCTCTTCTGAGA50.1142334932602239No Hit
GTTGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1142334932602239No Hit
GTTGCCGGGGTACCACTATAATCTCTAATACTCAGAATTA50.1142334932602239No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGC50.1142334932602239No Hit
GTTGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1142334932602239No Hit
GTTGCCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG50.1142334932602239No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG405.6437784E-833.8160936
CGGGGCT200.002563307333.8160936
GCCGGGG1850.033.8160934
CCGGGGT405.6437784E-833.8160935
CCGGGGG800.033.8160935
GCCGGGC301.2180282E-533.816094
CGGGAAG251.771903E-433.816096
CCGGGGC358.3207306E-733.816095
CCGGGGA301.2180282E-533.816095
CCGGGAA301.2180282E-533.816095
TTGCCGG4100.032.991312
TGCCGGG3850.032.937753
GTTGCCG4400.032.663271
GCCGGGA1250.031.1108044
GCCGGGT501.0635631E-830.4344834
CCGGGAT402.3644534E-629.589085
CCGGGAG402.3644534E-629.589085
CGGGTCA305.1676127E-428.1800756
CCGGGTC305.1676127E-428.1800755
TTTTTGT451.7866777E-422.806234
Produced by FastQC (version 0.11.5)