##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526143_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4377
Sequences flagged as poor quality	0
Sequence length	40
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.955677404615034	30.0	21.0	33.0	4.0	35.0
2	18.51405071967101	19.0	4.0	30.0	4.0	35.0
3	17.044094128398445	15.0	4.0	30.0	4.0	34.0
4	16.631482750742517	16.0	4.0	28.0	4.0	33.0
5	15.223212245830478	11.0	4.0	27.0	4.0	33.0
6	14.660954992003655	9.0	4.0	26.0	4.0	33.0
7	14.330134795522047	4.0	4.0	26.0	4.0	33.0
8	14.10235320996116	4.0	4.0	26.0	4.0	33.0
9	13.28581220013708	4.0	4.0	25.0	4.0	32.0
10	12.594928032899245	4.0	4.0	24.0	4.0	32.0
11	12.064884624171807	4.0	4.0	23.0	4.0	33.0
12	11.247658213388165	4.0	4.0	20.0	4.0	31.0
13	10.506054375142792	4.0	4.0	16.0	4.0	30.0
14	10.845556317112177	4.0	4.0	17.0	4.0	31.0
15	10.695910440941285	4.0	4.0	16.0	4.0	32.0
16	8.601553575508339	4.0	4.0	4.0	4.0	26.0
17	8.227096184601326	4.0	4.0	4.0	4.0	24.0
18	8.117888965044552	4.0	4.0	4.0	4.0	24.0
19	7.796892848983322	4.0	4.0	4.0	4.0	23.0
20	7.8380169065570025	4.0	4.0	4.0	4.0	23.0
21	7.60703678318483	4.0	4.0	4.0	4.0	22.0
22	7.621658670322138	4.0	4.0	4.0	4.0	22.0
23	7.578021475896733	4.0	4.0	4.0	4.0	23.0
24	7.405300434087274	4.0	4.0	4.0	4.0	21.0
25	7.564541923692026	4.0	4.0	4.0	4.0	23.0
26	7.600868174548777	4.0	4.0	4.0	4.0	23.0
27	7.696595841900845	4.0	4.0	4.0	4.0	24.0
28	7.7710760795065115	4.0	4.0	4.0	4.0	24.0
29	7.221384509938314	4.0	4.0	4.0	4.0	21.0
30	7.549691569568197	4.0	4.0	4.0	4.0	23.0
31	8.05071967100754	4.0	4.0	4.0	4.0	27.0
32	7.925519762394334	4.0	4.0	4.0	4.0	26.0
33	7.960703678318483	4.0	4.0	4.0	4.0	27.0
34	7.968928489833219	4.0	4.0	4.0	4.0	27.0
35	7.835503769705277	4.0	4.0	4.0	4.0	27.0
36	7.632168151702079	4.0	4.0	4.0	4.0	26.0
37	7.559058716015536	4.0	4.0	4.0	4.0	25.0
38	7.511080648846241	4.0	4.0	4.0	4.0	26.0
39	7.361663239661869	4.0	4.0	4.0	4.0	24.0
40	7.41832305231894	4.0	4.0	4.0	4.0	26.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-19.739693986568987
1	2	-14.27115766178266
1	3	-13.363137210012209
1	4	-12.736473595848597
1	5	-10.89170863858364
1	6	-10.765136599511596
1	7	-10.514293345543347
1	8	-10.091750610500611
1	9	-9.407055097680097
1	10	-8.471768162393158
1	11	-8.107303113553112
1	12	-6.8767895299145305
1	13	-5.97269536019536
1	14	-6.241414835164836
1	15	-6.256097374847375
1	16	-3.895329670329671
1	17	-3.0716842185592172
1	18	-2.8206272893772892
1	19	-2.814392551892552
1	20	-2.98365766178266
1	21	-2.73667963980464
1	22	-2.812851037851037
1	23	-2.7084668803418817
1	24	-2.416254578754577
1	25	-2.622886141636142
1	26	-2.8063721001221005
1	27	-2.793177655677656
1	28	-2.8285332722832717
1	29	-2.5774763431013437
1	30	-2.5677999084249077
1	31	-3.17612561050061
1	32	-2.990937881562881
1	33	-3.0039987789987777
1	34	-2.877155830280831
1	35	-2.6063377594627593
1	36	-2.624217796092794
1	37	-2.3644459706959715
1	38	-2.7588560744810744
1	39	-2.5614850427350433
1	40	-2.4737637362637352
2	1	-12.311122557997559
2	2	-14.27115766178266
2	3	-13.363137210012209
2	4	-12.736473595848597
2	5	-10.89170863858364
2	6	-10.765136599511596
2	7	-10.514293345543347
2	8	-10.091750610500611
2	9	-9.407055097680097
2	10	-8.471768162393158
2	11	-8.107303113553112
2	12	-6.8767895299145305
2	13	-5.97269536019536
2	14	-6.241414835164836
2	15	-6.256097374847375
2	16	-3.895329670329671
2	17	-3.0716842185592172
2	18	-2.8206272893772892
2	19	-2.814392551892552
2	20	-2.98365766178266
2	21	-2.73667963980464
2	22	-2.812851037851037
2	23	-2.7084668803418817
2	24	-2.416254578754577
2	25	-2.622886141636142
2	26	-2.8063721001221005
2	27	-2.793177655677656
2	28	-2.8285332722832717
2	29	-2.5774763431013437
2	30	-2.5677999084249077
2	31	-3.17612561050061
2	32	-2.990937881562881
2	33	-3.0039987789987777
2	34	-2.877155830280831
2	35	-2.6063377594627593
2	36	-2.624217796092794
2	37	-2.3644459706959715
2	38	-2.7588560744810744
2	39	-2.5614850427350433
2	40	-2.4737637362637352
3	1	-2.0730273199023195
3	2	-8.437824328449326
3	3	-9.029803876678875
3	4	-4.403140262515263
3	5	-3.5583753052503067
3	6	-1.431803266178262
3	7	-5.680960012210013
3	8	1.7415827228327228
3	9	2.2596115689865695
3	10	-4.805101495726491
3	11	-2.4406364468864457
3	12	-2.8767895299145305
3	13	-2.3060286935286927
3	14	-1.5747481684981697
3	15	-0.25609737484737494
3	16	-1.7286630036630042
3	17	-0.5716842185592172
3	18	2.346039377289377
3	19	2.852274114774114
3	20	5.183009004884006
3	21	2.596653693528694
3	22	3.5204822954822967
3	23	2.2915331196581183
3	24	6.083745421245423
3	25	4.543780525030524
3	26	4.5269612332112334
3	27	5.206822344322344
3	28	6.338133394383394
3	29	2.5891903235653224
3	30	0.09886675824175928
3	31	1.657207722832724
3	32	6.009062118437119
3	33	5.162667887667888
3	34	3.789510836385835
3	35	4.393662240537241
3	36	8.375782203907207
3	37	7.6355540293040285
3	38	7.907810592185592
3	39	5.771848290598291
3	40	8.359569597069598
4	1	-8.139693986568988
4	2	-9.27115766178266
4	3	-7.563137210012208
4	4	-1.1364735958485976
4	5	-7.49170863858364
4	6	-8.565136599511597
4	7	-8.314293345543348
4	8	-4.2917506105006105
4	9	-3.2070550976800973
4	10	-8.471768162393158
4	11	-8.107303113553112
4	12	-6.8767895299145305
4	13	-5.97269536019536
4	14	-6.241414835164836
4	15	-6.256097374847375
4	16	-3.895329670329671
4	17	-3.0716842185592172
4	18	-2.8206272893772892
4	19	-2.814392551892552
4	20	-2.98365766178266
4	21	-2.73667963980464
4	22	-2.812851037851037
4	23	-2.7084668803418817
4	24	-2.416254578754577
4	25	-2.622886141636142
4	26	-2.8063721001221005
4	27	-2.793177655677656
4	28	-2.8285332722832717
4	29	-2.5774763431013437
4	30	-2.5677999084249077
4	31	-3.17612561050061
4	32	-2.990937881562881
4	33	-3.0039987789987777
4	34	-2.877155830280831
4	35	-2.6063377594627593
4	36	-2.624217796092794
4	37	-2.3644459706959715
4	38	-2.7588560744810744
4	39	-2.5614850427350433
4	40	-2.4737637362637352
5	1	10.660306013431011
5	2	5.92884233821734
5	3	7.03686278998779
5	4	16.463526404151406
5	5	7.108291361416359
5	6	16.634863400488403
5	7	4.085706654456654
5	8	5.7082493894993895
5	9	10.392944902319904
5	10	15.928231837606841
5	11	-2.507303113553112
5	12	-1.2767895299145309
5	13	0.027304639804640374
5	14	-2.441414835164836
5	15	-0.25609737484737494
5	16	1.3046703296703281
5	17	-3.0716842185592172
5	18	-2.8206272893772892
5	19	-2.814392551892552
5	20	-2.98365766178266
5	21	-2.73667963980464
5	22	-2.812851037851037
5	23	-2.7084668803418817
5	24	-2.416254578754577
5	25	-2.622886141636142
5	26	-2.8063721001221005
5	27	-2.793177655677656
5	28	-2.8285332722832717
5	29	-2.5774763431013437
5	30	-2.5677999084249077
5	31	-3.17612561050061
5	32	-2.990937881562881
5	33	-3.0039987789987777
5	34	-2.877155830280831
5	35	-2.6063377594627593
5	36	-2.624217796092794
5	37	-2.3644459706959715
5	38	-2.7588560744810744
5	39	-2.5614850427350433
5	40	-2.4737637362637352
6	1	6.885306013431013
6	2	1.4788423382173406
6	3	7.2618627899877914
6	4	5.388526404151403
6	5	8.48329136141636
6	6	14.234863400488404
6	7	7.360706654456653
6	8	14.408249389499389
6	9	12.217944902319903
6	10	7.2782318376068424
6	11	16.51769688644689
6	12	13.62321047008547
6	13	10.77730463980464
6	14	10.633585164835164
6	15	9.868902625152625
6	16	11.729670329670329
6	17	8.553315781440784
6	18	8.054372710622712
6	19	7.935607448107448
6	20	9.51634233821734
6	21	6.88832036019536
6	22	9.062148962148964
6	23	5.166533119658118
6	24	8.333745421245423
6	25	6.002113858363858
6	26	2.3186278998778995
6	27	6.456822344322344
6	28	8.796466727716728
6	29	7.172523656898656
6	30	6.682200091575092
6	31	5.19887438949939
6	32	7.009062118437119
6	33	9.371001221001222
6	34	9.74784416971917
6	35	5.643662240537241
6	36	11.125782203907207
6	37	10.135554029304028
6	38	11.616143925518926
6	39	8.313514957264957
6	40	3.9012362637362648
7	1	2.0603060134310134
7	2	9.72884233821734
7	3	-1.9631372100122082
7	4	9.263526404151403
7	5	11.308291361416359
7	6	7.234863400488404
7	7	0.48570665445665284
7	8	8.108249389499388
7	9	2.5929449023199034
7	10	9.328231837606843
7	11	5.092696886446888
7	12	10.323210470085469
7	13	11.22730463980464
7	14	9.358585164835166
7	15	7.543902625152626
7	16	2.504670329670329
7	17	9.128315781440783
7	18	10.979372710622712
7	19	14.785607448107449
7	20	12.616342338217342
7	21	11.663320360195359
7	22	13.987148962148964
7	23	9.09153311965812
7	24	8.383745421245424
7	25	13.177113858363859
7	26	13.393627899877899
7	27	18.406822344322343
7	28	8.171466727716728
7	29	13.022523656898658
7	30	9.432200091575092
7	31	17.423874389499392
7	32	17.80906211843712
7	33	15.996001221001222
7	34	17.72284416971917
7	35	20.593662240537242
7	36	16.375782203907207
7	37	20.43555402930403
7	38	11.441143925518926
7	39	11.238514957264957
7	40	14.126236263736267
8	1	3.2603060134310127
8	2	0.9288423382173399
8	3	8.636862789987791
8	4	14.063526404151403
8	5	14.10829136141636
8	6	13.434863400488403
8	7	16.485706654456653
8	8	14.50824938949939
8	9	16.392944902319904
8	10	16.92823183760684
8	11	3.8926968864468883
8	12	7.92321047008547
8	13	6.427304639804639
8	14	8.358585164835166
8	15	7.543902625152626
8	16	11.70467032967033
8	17	7.928315781440783
8	18	10.779372710622713
8	19	5.785607448107448
8	20	9.616342338217342
8	21	9.863320360195361
8	22	9.787148962148965
8	23	10.29153311965812
8	24	0.1837454212454226
8	25	5.377113858363858
8	26	8.1936278998779
8	27	11.406822344322343
8	28	10.371466727716728
8	29	6.822523656898657
8	30	7.232200091575093
8	31	12.423874389499392
8	32	10.809062118437119
8	33	13.196001221001222
8	34	12.322844169719168
8	35	11.79366224053724
8	36	8.775782203907205
8	37	12.635554029304028
8	38	13.041143925518927
8	39	13.438514957264957
8	40	12.526236263736266
9	1	10.06030601343101
9	2	10.328842338217342
9	3	4.6368627899877914
9	4	14.463526404151402
9	5	11.10829136141636
9	6	10.234863400488404
9	7	4.085706654456654
9	8	-3.691750610500611
9	9	0.5929449023199034
9	10	-1.4717681623931576
9	11	0.4926968864468879
9	12	7.323210470085469
9	13	1.4273046398046407
9	14	8.758585164835164
9	15	4.543902625152626
9	16	2.7046703296703285
9	17	-1.2716842185592174
9	18	5.37937271062271
9	19	0.7856074481074478
9	20	-1.9836576617826598
9	21	-1.5366796398046398
9	22	-0.21285103785103754
9	23	-0.9084668803418818
9	24	-0.21625457875457688
9	25	-1.2228861416361418
9	26	-1.2063721001221008
9	27	1.0068223443223436
9	28	2.1714667277167283
9	29	1.2225236568986562
9	30	-0.7677999084249079
9	31	2.82387438949939
9	32	-0.19093788156288127
9	33	2.796001221001223
9	34	3.122844169719169
9	35	-0.006337759462759607
9	36	0.17578220390720567
9	37	3.835554029304028
9	38	3.441143925518925
9	39	2.2385149572649574
9	40	0.3262362637362646
10	1	1.8158615689865698
10	2	-5.493379884004881
10	3	-7.807581654456653
10	4	-2.2920291514041526
10	5	-6.002819749694751
10	6	-5.654025488400485
10	7	-1.9587377899877918
10	8	-0.6473061660561665
10	9	-4.295943986568986
10	10	-0.5828792735042683
10	11	2.5593635531135543
10	12	3.567654914529914
10	13	3.5828601953601957
10	14	6.091918498168496
10	15	0.2994581807081804
10	16	-1.2286630036630042
10	17	0.3727602258852274
10	18	1.8460393772893768
10	19	1.9633852258852267
10	20	1.7941201159951188
10	21	0.3744314713064707
10	22	0.40937118437118514
10	23	1.1804220085470076
10	24	2.250412087912089
10	25	1.4882249694749685
10	26	0.0825167887667888
10	27	0.5401556776556768
10	28	2.0603556166056176
10	29	-0.799698565323566
10	30	1.0988667582417593
10	31	3.6016521672771686
10	32	2.7868398962148975
10	33	3.440445665445667
10	34	3.9006219474969477
10	35	2.949217796092796
10	36	2.5980044261294273
10	37	2.3022206959706946
10	38	2.130032814407815
10	39	3.5496260683760674
10	40	3.8595695970695987
11	1	9.546020299145297
11	2	-3.8425862332112306
11	3	3.922577075702076
11	4	4.69209783272283
11	5	-7.034565781440784
11	6	-5.050850885225882
11	7	-1.942864774114776
11	8	-7.234607753357754
11	9	-6.264197954822953
11	10	-2.757482448107444
11	11	0.03555402930403062
11	12	0.26606761294261183
11	13	-0.40126678876678845
11	14	-0.6699862637362646
11	15	-2.1132402319902326
11	16	-2.752472527472528
11	17	-1.7859699328449317
11	18	-0.10634157509157482
11	19	-2.2429639804639807
11	20	-0.555086233211231
11	21	-0.16525106837606884
11	22	0.18714896214896282
11	23	0.1486759768009751
11	24	0.583745421245423
11	25	0.23425671550671456
11	26	1.3364850427350419
11	27	0.06396520146520057
11	28	0.6000381562881572
11	29	-1.1489049145299148
11	30	1.5750572344322347
11	31	1.1095886752136765
11	32	-1.5623664529914523
11	33	1.2817155067155088
11	34	0.5514155982905979
11	35	0.9650908119658119
11	36	-1.3385035103785086
11	37	-1.3644459706959715
11	38	-0.33028464590964557
11	39	1.4385149572649567
11	40	0.669093406593408
12	1	-4.139693986568986
12	2	1.1288423382173391
12	3	-9.963137210012208
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106	26	-2.8063721001221005
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106	29	-2.5774763431013437
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106	32	-2.990937881562881
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106	34	-2.877155830280831
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107	2	2.2288423382173406
107	3	7.8868627899877914
107	4	-7.236473595848597
107	5	0.10829136141635942
107	6	-6.265136599511596
107	7	2.485706654456653
107	8	2.158249389499389
107	9	-2.9070550976800966
107	10	-1.2217681623931576
107	11	-8.107303113553112
107	12	-6.8767895299145305
107	13	-5.97269536019536
107	14	-6.241414835164836
107	15	-6.256097374847375
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107	20	-2.98365766178266
107	21	-2.73667963980464
107	22	-2.812851037851037
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107	27	-2.793177655677656
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107	29	-2.5774763431013437
107	30	-2.5677999084249077
107	31	-3.17612561050061
107	32	-2.990937881562881
107	33	-3.0039987789987777
107	34	-2.877155830280831
107	35	-2.6063377594627593
107	36	-2.624217796092794
107	37	-2.3644459706959715
107	38	-2.7588560744810744
107	39	-2.5614850427350433
107	40	-2.4737637362637352
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108	2	-2.2711576617826594
108	3	-7.8631372100122086
108	4	-3.2364735958485973
108	5	-0.3917086385836406
108	6	-7.765136599511596
108	7	-7.514293345543347
108	8	-5.591750610500611
108	9	3.5929449023199034
108	10	-5.4717681623931576
108	11	4.892696886446888
108	12	3.1232104700854695
108	13	3.5273046398046404
108	14	8.258585164835164
108	15	-6.256097374847375
108	16	-3.895329670329671
108	17	-3.0716842185592172
108	18	-2.8206272893772892
108	19	-2.814392551892552
108	20	-2.98365766178266
108	21	-2.73667963980464
108	22	-2.812851037851037
108	23	-2.7084668803418817
108	24	-2.416254578754577
108	25	-2.622886141636142
108	26	-2.8063721001221005
108	27	-2.793177655677656
108	28	-2.8285332722832717
108	29	-2.5774763431013437
108	30	-2.5677999084249077
108	31	-3.17612561050061
108	32	-2.990937881562881
108	33	-3.0039987789987777
108	34	-2.877155830280831
108	35	-2.6063377594627593
108	36	-2.624217796092794
108	37	-2.3644459706959715
108	38	-2.7588560744810744
108	39	-2.5614850427350433
108	40	-2.4737637362637352
110	1	4.010306013431013
110	2	-0.7711576617826594
110	3	9.136862789987791
110	4	6.013526404151403
110	5	5.608291361416359
110	6	-3.515136599511596
110	7	-8.014293345543347
110	8	-2.841750610500611
110	9	-7.407055097680097
110	10	-1.9717681623931576
110	11	-8.107303113553112
110	12	-6.8767895299145305
110	13	-5.97269536019536
110	14	-6.241414835164836
110	15	-6.256097374847375
110	16	-3.895329670329671
110	17	-3.0716842185592172
110	18	-2.8206272893772892
110	19	-2.814392551892552
110	20	-2.98365766178266
110	21	-2.73667963980464
110	22	-2.812851037851037
110	23	-2.7084668803418817
110	24	-2.416254578754577
110	25	-2.622886141636142
110	26	-2.8063721001221005
110	27	-2.793177655677656
110	28	-2.8285332722832717
110	29	-2.5774763431013437
110	30	-2.5677999084249077
110	31	-3.17612561050061
110	32	-2.990937881562881
110	33	-3.0039987789987777
110	34	-2.877155830280831
110	35	-2.6063377594627593
110	36	-2.624217796092794
110	37	-2.3644459706959715
110	38	-2.7588560744810744
110	39	-2.5614850427350433
110	40	-2.4737637362637352
111	1	9.660306013431011
111	2	14.328842338217342
111	3	12.636862789987791
111	4	8.463526404151402
111	5	8.508291361416358
111	6	6.8348634004884055
111	7	6.2857066544566536
111	8	5.308249389499387
111	9	8.392944902319904
111	10	4.128231837606844
111	11	4.692696886446889
111	12	9.323210470085469
111	13	3.827304639804641
111	14	0.1585851648351646
111	15	5.143902625152625
111	16	0.9046703296703296
111	17	1.7283157814407835
111	18	-1.4206272893772889
111	19	-1.6143925518925517
111	20	-1.7836576617826596
111	21	2.6633203601953603
111	22	2.587148962148963
111	23	1.6915331196581187
111	24	1.9837454212454233
111	25	2.777113858363858
111	26	3.393627899877899
111	27	2.606822344322344
111	28	2.5714667277167287
111	29	2.8225236568986567
111	30	1.4322000915750923
111	31	3.0238743894993894
111	32	2.4090621184371193
111	33	3.1960012210012216
111	34	3.3228441697191684
111	35	2.793662240537241
111	36	-2.624217796092794
111	37	-2.3644459706959715
111	38	-2.7588560744810744
111	39	-2.5614850427350433
111	40	-2.4737637362637352
112	1	6.760306013431013
112	2	11.22884233821734
112	3	-10.363137210012209
112	4	1.7635264041514027
112	5	-10.89170863858364
112	6	-10.765136599511596
112	7	-10.514293345543347
112	8	-10.091750610500611
112	9	-9.407055097680097
112	10	-8.471768162393158
112	11	-8.107303113553112
112	12	-6.8767895299145305
112	13	-5.97269536019536
112	14	-6.241414835164836
112	15	-6.256097374847375
112	16	-3.895329670329671
112	17	-3.0716842185592172
112	18	-2.8206272893772892
112	19	-2.814392551892552
112	20	-2.98365766178266
112	21	-2.73667963980464
112	22	-2.812851037851037
112	23	-2.7084668803418817
112	24	-2.416254578754577
112	25	-2.622886141636142
112	26	-2.8063721001221005
112	27	-2.793177655677656
112	28	-2.8285332722832717
112	29	-2.5774763431013437
112	30	-2.5677999084249077
112	31	-3.17612561050061
112	32	-2.990937881562881
112	33	-3.0039987789987777
112	34	-2.877155830280831
112	35	-2.6063377594627593
112	36	-2.624217796092794
112	37	-2.3644459706959715
112	38	-2.7588560744810744
112	39	-2.5614850427350433
112	40	-2.4737637362637352
113	1	2.860306013431014
113	2	-6.871157661782659
113	3	-2.3631372100122086
113	4	-11.136473595848598
113	5	-9.291708638583641
113	6	-4.965136599511595
113	7	-7.9142933455433475
113	8	-7.4917506105006115
113	9	-6.2070550976800964
113	10	-2.671768162393157
113	11	-8.107303113553112
113	12	-6.8767895299145305
113	13	-5.97269536019536
113	14	-6.241414835164836
113	15	-6.256097374847375
113	16	-3.895329670329671
113	17	-3.0716842185592172
113	18	-2.8206272893772892
113	19	-2.814392551892552
113	20	-2.98365766178266
113	21	-2.73667963980464
113	22	-2.812851037851037
113	23	-2.7084668803418817
113	24	-2.416254578754577
113	25	-2.622886141636142
113	26	-2.8063721001221005
113	27	-2.793177655677656
113	28	-2.8285332722832717
113	29	-2.5774763431013437
113	30	-2.5677999084249077
113	31	-3.17612561050061
113	32	-2.990937881562881
113	33	-3.0039987789987777
113	34	-2.877155830280831
113	35	-2.6063377594627593
113	36	-2.624217796092794
113	37	-2.3644459706959715
113	38	-2.7588560744810744
113	39	-2.5614850427350433
113	40	-2.4737637362637352
114	1	-0.9896939865689873
114	2	3.7288423382173406
114	3	5.8868627899877914
114	4	-11.236473595848597
114	5	-4.141708638583641
114	6	-4.265136599511596
114	7	-4.514293345543347
114	8	-5.591750610500611
114	9	-6.907055097680097
114	10	-1.2217681623931576
114	11	-3.3573031135531117
114	12	-4.8767895299145305
114	13	-4.72269536019536
114	14	-5.241414835164836
114	15	-5.256097374847375
114	16	-3.395329670329671
114	17	0.17831578144078275
114	18	-1.8206272893772892
114	19	0.4356074481074481
114	20	-1.7336576617826598
114	21	3.26332036019536
114	22	2.687148962148963
114	23	4.041533119658118
114	24	2.583745421245423
114	25	2.877113858363858
114	26	5.6936278998778995
114	27	5.706822344322344
114	28	5.671466727716728
114	29	5.922523656898656
114	30	4.682200091575092
114	31	3.82387438949939
114	32	5.259062118437119
114	33	5.496001221001222
114	34	5.622844169719169
114	35	1.8936622405372407
114	36	-0.37421779609279415
114	37	-0.8644459706959715
114	38	3.2411439255189256
114	39	4.688514957264957
114	40	4.026236263736265
115	1	-1.2396939865689873
115	2	-6.271157661782659
115	3	-4.8631372100122086
115	4	-4.736473595848597
115	5	-1.7250419719169745
115	6	-9.265136599511596
115	7	-8.514293345543347
115	8	-8.925083943833943
115	9	-8.407055097680097
115	10	-4.6384348290598245
115	11	-2.773969780219778
115	12	-2.3767895299145305
115	13	-1.4726953601953596
115	14	-1.9080815018315018
115	15	-6.256097374847375
115	16	-3.895329670329671
115	17	-3.0716842185592172
115	18	-2.8206272893772892
115	19	-2.814392551892552
115	20	-2.98365766178266
115	21	-2.73667963980464
115	22	-2.812851037851037
115	23	-2.7084668803418817
115	24	-2.416254578754577
115	25	-2.622886141636142
115	26	-2.8063721001221005
115	27	-2.793177655677656
115	28	-2.8285332722832717
115	29	-2.5774763431013437
115	30	-2.5677999084249077
115	31	-3.17612561050061
115	32	-2.990937881562881
115	33	-3.0039987789987777
115	34	-2.877155830280831
115	35	-2.6063377594627593
115	36	-2.624217796092794
115	37	-2.3644459706959715
115	38	-2.7588560744810744
115	39	-2.5614850427350433
115	40	-2.4737637362637352
116	1	9.260306013431013
116	2	8.97884233821734
116	3	-0.6131372100122086
116	4	6.263526404151403
116	5	0.6082913614163594
116	6	-0.01513659951159596
116	7	2.235706654456653
116	8	-1.3417506105006112
116	9	2.0929449023199034
116	10	-3.7217681623931576
116	11	-2.6073031135531117
116	12	-0.12678952991453052
116	13	-0.22269536019535963
116	14	-0.24141483516483575
116	15	0.49390262515262506
116	16	-3.895329670329671
116	17	-3.0716842185592172
116	18	-2.8206272893772892
116	19	-2.814392551892552
116	20	-2.98365766178266
116	21	-2.73667963980464
116	22	-2.812851037851037
116	23	-2.7084668803418817
116	24	-2.416254578754577
116	25	-2.622886141636142
116	26	-2.8063721001221005
116	27	-2.793177655677656
116	28	-2.8285332722832717
116	29	-2.5774763431013437
116	30	-2.5677999084249077
116	31	-3.17612561050061
116	32	-2.990937881562881
116	33	-3.0039987789987777
116	34	-2.877155830280831
116	35	-2.6063377594627593
116	36	-2.624217796092794
116	37	-2.3644459706959715
116	38	-2.7588560744810744
116	39	-2.5614850427350433
116	40	-2.4737637362637352
117	1	10.593639346764348
117	2	15.062175671550676
117	3	15.970196123321127
117	4	17.930193070818067
117	5	12.441624694749692
117	6	6.901530067155072
117	7	11.485706654456653
117	8	17.24158272283272
117	9	17.25961156898657
117	10	7.5282318376068424
117	11	11.892696886446888
117	12	8.12321047008547
117	13	7.3606379731379725
117	14	10.758585164835164
117	15	9.410569291819293
117	16	5.104670329670329
117	17	-3.0716842185592172
117	18	-2.8206272893772892
117	19	-2.814392551892552
117	20	-2.98365766178266
117	21	-2.73667963980464
117	22	-2.812851037851037
117	23	-2.7084668803418817
117	24	-2.416254578754577
117	25	-2.622886141636142
117	26	-2.8063721001221005
117	27	-2.793177655677656
117	28	-2.8285332722832717
117	29	-2.5774763431013437
117	30	-2.5677999084249077
117	31	-3.17612561050061
117	32	-2.990937881562881
117	33	-3.0039987789987777
117	34	-2.877155830280831
117	35	-2.6063377594627593
117	36	-2.624217796092794
117	37	-2.3644459706959715
117	38	-2.7588560744810744
117	39	-2.5614850427350433
117	40	-2.4737637362637352
119	1	9.260306013431013
119	2	16.062175671550676
119	3	15.636862789987791
119	4	14.93019307081807
119	5	10.774958028083027
119	6	8.568196733821736
119	7	15.15237332112332
119	8	18.90824938949939
119	9	18.92627823565324
119	10	12.861565170940175
119	11	8.22603021978022
119	12	9.456543803418802
119	13	3.6939713064713064
119	14	-6.241414835164836
119	15	-6.256097374847375
119	16	-3.895329670329671
119	17	-3.0716842185592172
119	18	-2.8206272893772892
119	19	-2.814392551892552
119	20	-2.98365766178266
119	21	-2.73667963980464
119	22	-2.812851037851037
119	23	-2.7084668803418817
119	24	-2.416254578754577
119	25	-2.622886141636142
119	26	-2.8063721001221005
119	27	-2.793177655677656
119	28	-2.8285332722832717
119	29	-2.5774763431013437
119	30	-2.5677999084249077
119	31	-3.17612561050061
119	32	-2.990937881562881
119	33	-3.0039987789987777
119	34	-2.877155830280831
119	35	-2.6063377594627593
119	36	-2.624217796092794
119	37	-2.3644459706959715
119	38	-2.7588560744810744
119	39	-2.5614850427350433
119	40	-2.4737637362637352
120	1	-7.239693986568987
120	2	0.22884233821734057
120	3	-1.3631372100122086
120	4	1.7635264041514027
120	5	-2.8917086385836406
120	6	-5.765136599511596
120	7	-9.014293345543347
120	8	-5.091750610500611
120	9	-2.9070550976800966
120	10	-4.7217681623931576
120	11	-3.1073031135531117
120	12	-5.8767895299145305
120	13	-0.7226953601953596
120	14	-1.9914148351648358
120	15	0.24390262515262506
120	16	-3.895329670329671
120	17	-3.0716842185592172
120	18	-2.8206272893772892
120	19	-2.814392551892552
120	20	-2.98365766178266
120	21	-2.73667963980464
120	22	-2.812851037851037
120	23	-2.7084668803418817
120	24	-2.416254578754577
120	25	-2.622886141636142
120	26	-2.8063721001221005
120	27	-2.793177655677656
120	28	-2.8285332722832717
120	29	-2.5774763431013437
120	30	-2.5677999084249077
120	31	-3.17612561050061
120	32	-2.990937881562881
120	33	-3.0039987789987777
120	34	-2.877155830280831
120	35	-2.6063377594627593
120	36	-2.624217796092794
120	37	-2.3644459706959715
120	38	-2.7588560744810744
120	39	-2.5614850427350433
120	40	-2.4737637362637352
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1428.0
5	346.0
6	288.0
7	238.0
8	188.0
9	246.0
10	202.0
11	191.0
12	118.0
13	105.0
14	89.0
15	57.0
16	52.0
17	25.0
18	34.0
19	41.0
20	69.0
21	110.0
22	122.0
23	121.0
24	106.0
25	69.0
26	59.0
27	39.0
28	16.0
29	12.0
30	5.0
31	0.0
32	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	98.46857142857142	0.64	0.43428571428571433	0.4571428571428572
8	96.34034766697164	1.898444647758463	1.3723696248856359	0.3888380603842635
9	93.14377436367806	2.637009860123825	2.614079339601009	1.6051364365971106
10	46.58129430596844	24.102446832837867	17.059227075234393	12.257031785959295
11	31.794285714285714	20.457142857142856	22.01142857142857	25.737142857142857
12	30.111948823395018	26.136623257939227	23.829106694082704	19.922321224583047
13	23.692026502170435	22.70961846013251	24.58304774960018	29.01530728809687
14	20.927575965273018	32.32807859264336	28.946767192140737	17.797578249942884
15	33.12771304546493	23.235092529129542	28.74114690427233	14.896047521133196
16	30.180488919351156	30.957276673520678	24.62874114690427	14.233493260223899
17	27.10237659963437	23.26325411334552	32.38117001828154	17.253199268738573
18	26.822024217500573	24.651587845556318	21.864290610006854	26.66209732693626
19	25.040018294077292	28.424422593185454	23.645094900525955	22.8904642122113
20	27.00479780671693	27.62165867032214	32.579392277815856	12.794151245145077
21	24.925748229380854	24.148960475211332	27.393191683801692	23.53209961160612
22	19.853781128626913	23.37217272104181	37.057345213616635	19.716700936714645
23	22.22983778843957	26.547863833676033	33.721727210418095	17.5005711674663
24	22.582857142857144	26.56	24.45714285714286	26.400000000000002
25	22.250171506974617	26.48067688085982	29.339126457809282	21.93002515435628
26	29.752633989922128	27.691250572606506	23.52267521759047	19.033440219880898
27	19.728984841525037	33.46348185576481	24.00091869545246	22.806614607257693
28	22.435458076307974	35.64084989718986	22.618231665524334	19.30546036097784
29	26.582568807339452	32.11009174311927	23.41743119266055	17.889908256880734
30	27.484578478409873	26.365090244459676	28.44413982179575	17.706191455334704
31	28.421293123143705	28.535526616403928	25.33698880511766	17.706191455334704
32	29.88321502175407	26.173574536294943	26.837645981222806	17.10556446072819
33	29.632168151702075	27.827278958190544	26.844870916152612	15.695681973954764
34	18.962759881197165	25.839616175462645	40.18734292894677	15.01028101439342
35	18.757139593328766	30.02056202878684	36.28055745944711	14.941740918437285
36	22.293430991073475	31.792172121766992	28.793774319066145	17.120622568093385
37	25.93100297007083	29.97486863148275	28.23851953392735	15.855608864519077
38	25.359835503769705	31.29997715330135	27.05049120402102	16.289696138907928
39	26.08	29.25714285714286	22.14857142857143	22.514285714285716
40	23.989033584647018	27.57596527301805	27.3474982864976	21.087502855837332
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	4.5
12	9.0
13	9.0
14	9.0
15	9.0
16	19.5
17	30.0
18	30.0
19	31.5
20	33.0
21	32.0
22	31.0
23	31.0
24	25.5
25	20.0
26	22.0
27	24.0
28	24.0
29	33.5
30	43.0
31	50.0
32	57.0
33	57.0
34	66.0
35	75.0
36	90.5
37	106.0
38	106.0
39	151.5
40	197.0
41	208.0
42	219.0
43	219.0
44	243.5
45	268.0
46	321.5
47	375.0
48	375.0
49	354.5
50	334.0
51	333.0
52	332.0
53	332.0
54	371.5
55	411.0
56	440.0
57	469.0
58	469.0
59	441.0
60	413.0
61	382.0
62	351.0
63	351.0
64	330.0
65	309.0
66	223.0
67	137.0
68	137.0
69	106.0
70	75.0
71	51.5
72	28.0
73	28.0
74	20.0
75	12.0
76	8.0
77	4.0
78	4.0
79	4.0
80	4.0
81	3.0
82	2.0
83	2.0
84	1.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.04569339730408956
8	0.1142334932602239
9	0.3655471784327165
10	0.09138679460817913
11	0.04569339730408956
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.02284669865204478
18	0.0
19	0.09138679460817913
20	0.0
21	0.0
22	0.0
23	0.0
24	0.04569339730408956
25	0.09138679460817913
26	0.2513136851724926
27	0.52547406899703
28	0.0
29	0.38839387708476125
30	0.0
31	0.0
32	0.2284669865204478
33	0.0
34	0.0
35	0.0
36	0.18277358921635825
37	0.0
38	0.0
39	0.04569339730408956
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	4377.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.62417180717387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.11555075593952	78.79826365090244
2	4.508639308855292	7.630797349782957
3	0.9989200863930885	2.5359835503769705
4	0.5669546436285098	1.9191226867717615
5	0.16198704103671707	0.6854009595613434
6	0.21598272138228944	1.0966415352981496
7	0.0	0.0
8	0.08099352051835854	0.5483207676490748
9	0.05399568034557236	0.411240575736806
>10	0.24298056155507558	3.335618003198538
>50	0.05399568034557236	3.0386109207219554
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTTGCCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT	70	1.5992689056431346	No Hit
GTTGCCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG	63	1.439342015078821	No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	32	0.731094356865433	No Hit
GTTGCCGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC	20	0.4569339730408956	No Hit
GTTGCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18	0.411240575736806	No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA	14	0.3198537811286269	No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	13	0.29700708247658214	No Hit
GTTGCCGGGGTTTGTGACGGGGGGCTGGGGACTCTCTCTT	13	0.29700708247658214	No Hit
GTTGCCGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG	12	0.2741603838245374	No Hit
GTTGCCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT	12	0.2741603838245374	No Hit
GTTGCCGGGACTAATCCTAGCCCTAGCCCTACACAAATAT	12	0.2741603838245374	No Hit
GTTGCCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9	0.205620287868403	No Hit
GTTGCCGGGGCTGTTGCCGGGTCACAGATCCTCTTCTGAG	9	0.205620287868403	No Hit
GTTGCCGGGGCTCCTGGGAGGGGCGTTCTCCTGGCTTGAG	8	0.18277358921635825	No Hit
GTTGCCGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT	8	0.18277358921635825	No Hit
GTTGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA	8	0.18277358921635825	No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC	6	0.1370801919122687	No Hit
GTTGCCGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC	6	0.1370801919122687	No Hit
GTTGCCGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAGC	6	0.1370801919122687	No Hit
GTTGCCGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG	6	0.1370801919122687	No Hit
GTTGCCGTCTTCTGCTTGGATCGGAAGAGCTCGTATGCCG	6	0.1370801919122687	Illumina Single End Adapter 2 (95% over 23bp)
GTTGCCGGGGGACTGAGGTGTGGGTGTGTTCTATATTCCA	6	0.1370801919122687	No Hit
GTTGCCGGGGCTGTTGCCGGGTCACAGATCCTCTTCTGAA	6	0.1370801919122687	No Hit
GTTGCCGGGGTGTTCTTTATTAATATCCTAACACTCCTCG	6	0.1370801919122687	No Hit
GTTGCCGGGCTGTTGCCGGGTCACAGATCCTCTTCTGAGA	5	0.1142334932602239	No Hit
GTTGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.1142334932602239	No Hit
GTTGCCGGGGTACCACTATAATCTCTAATACTCAGAATTA	5	0.1142334932602239	No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGC	5	0.1142334932602239	No Hit
GTTGCCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5	0.1142334932602239	No Hit
GTTGCCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG	5	0.1142334932602239	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.045693397304089556	0.0	0.0	0.0	0.0
15	0.045693397304089556	0.0	0.0	0.0	0.0
16	0.045693397304089556	0.0	0.0	0.0	0.0
17	0.045693397304089556	0.0	0.0	0.0	0.0
18	0.045693397304089556	0.0	0.0	0.0	0.0
19	0.045693397304089556	0.0	0.0	0.0	0.0
20	0.045693397304089556	0.0	0.0	0.0	0.0
21	0.045693397304089556	0.0	0.0	0.0	0.0
22	0.045693397304089556	0.0	0.0	0.0	0.0
23	0.045693397304089556	0.0	0.0	0.0	0.0
24	0.045693397304089556	0.0	0.0	0.0	0.0
25	0.045693397304089556	0.0	0.0	0.0	0.0
26	0.045693397304089556	0.0	0.0	0.0	0.0
27	0.06854009595613433	0.0	0.0	0.0	0.0
28	0.06854009595613433	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGGGG	40	5.6437784E-8	33.816093	6
CGGGGCT	20	0.0025633073	33.816093	6
GCCGGGG	185	0.0	33.816093	4
CCGGGGT	40	5.6437784E-8	33.816093	5
CCGGGGG	80	0.0	33.816093	5
GCCGGGC	30	1.2180282E-5	33.81609	4
CGGGAAG	25	1.771903E-4	33.81609	6
CCGGGGC	35	8.3207306E-7	33.81609	5
CCGGGGA	30	1.2180282E-5	33.81609	5
CCGGGAA	30	1.2180282E-5	33.81609	5
TTGCCGG	410	0.0	32.99131	2
TGCCGGG	385	0.0	32.93775	3
GTTGCCG	440	0.0	32.66327	1
GCCGGGA	125	0.0	31.110804	4
GCCGGGT	50	1.0635631E-8	30.434483	4
CCGGGAT	40	2.3644534E-6	29.58908	5
CCGGGAG	40	2.3644534E-6	29.58908	5
CGGGTCA	30	5.1676127E-4	28.180075	6
CCGGGTC	30	5.1676127E-4	28.180075	5
TTTTTGT	45	1.7866777E-4	22.8062	34
>>END_MODULE