FastQCFastQC Report
Fri 17 Jun 2016
SRR1526142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14421
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT1511.0470841134456697No Hit
CACAAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC1010.7003675195894876No Hit
CACAAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC390.2704389432078219No Hit
CACAAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA360.249635947576451No Hit
CACAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT340.23576728382220372No Hit
CACAAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT320.22189862006795646No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC290.20109562443658552No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC270.18722696068233824No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC270.18722696068233824No Hit
CACAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC270.18722696068233824No Hit
CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG200.1386866375424728No Hit
CACAAGGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCC190.13175230566534915No Hit
CACAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATCATTATACTATTATAT180.1248179737882255No Hit
CACAAGGGGGTGTACTCTGTATTATCGTTCTGATGCCAAATGAAGATACAACTGAA180.1248179737882255No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC160.11094931003397823No Hit
CACAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.10401497815685459No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGACT150.007525293651.18996441
CGGATTG401.9226718E-951.18996439
GCGGATT401.9226718E-951.18996438
AACCAAC150.007525293651.18996441
GCGCATT150.007525293651.18996438
ACAACAA150.007525293651.18996439
GGCGCAT150.00763307551.0071437
GGCGGAT401.9863364E-951.0071437
AAATTAC150.007742001750.82562334
TTGGGCG650.050.82562334
TCAAACC150.007742001750.82562335
ATGCTTG150.007742001750.82562336
TGGGCGC150.007742001750.82562335
GGGCGCA150.007742001750.82562336
TGACTGC150.00796331950.4664343
GTGGTAT204.070981E-449.9370615
GGTTGTT150.00842003649.763079
TATCAAC301.0092663E-649.7630719
TCCCCGC150.00842003649.7630710
TCACAGA800.049.76306510
Produced by FastQC (version 0.11.5)