##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526142_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14421 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.9529852298731 38.0 37.0 40.0 33.0 40.0 2 35.37306705498925 38.0 36.0 39.0 26.0 40.0 3 35.28860689272589 38.0 35.0 40.0 25.0 40.0 4 34.58498023715415 38.0 35.0 39.0 17.0 40.0 5 34.883017821232926 38.0 35.0 39.0 23.0 40.0 6 34.78260869565217 38.0 35.0 39.0 23.0 40.0 7 35.802302198183206 38.0 35.0 39.0 28.0 40.0 8 35.62797309479232 38.0 35.0 39.0 27.0 40.0 9 35.64232716177796 38.0 35.0 39.0 27.0 40.0 10 34.693363844393595 38.0 34.0 39.0 24.0 40.0 11 34.53283406143818 38.0 33.0 39.0 26.0 40.0 12 33.657652035226405 38.0 32.0 39.0 21.0 40.0 13 33.25490603980307 37.0 32.0 39.0 19.0 40.0 14 34.02302198183205 38.0 33.0 39.0 23.0 40.0 15 32.95076624367242 37.0 31.0 39.0 18.0 40.0 16 32.415435822758475 36.0 29.0 39.0 19.0 40.0 17 31.31239165106442 35.0 27.0 39.0 15.0 40.0 18 30.58276125095347 35.0 25.0 38.0 13.0 40.0 19 31.95340128978573 36.0 28.0 39.0 17.0 40.0 20 32.44067679079121 36.0 29.0 39.0 20.0 40.0 21 33.002843076069624 37.0 30.0 39.0 19.0 40.0 22 32.25539144303446 36.0 29.0 39.0 16.0 40.0 23 33.03536509257333 38.0 31.0 39.0 19.0 40.0 24 32.31162887455794 36.0 29.0 39.0 16.0 40.0 25 32.93266763747313 37.0 31.0 39.0 19.0 40.0 26 33.42736287358713 38.0 33.0 39.0 19.0 40.0 27 33.65071770334928 38.0 33.0 39.0 21.0 40.0 28 33.69752444351987 38.0 33.0 39.0 22.0 40.0 29 33.19839123500451 38.0 32.0 39.0 18.0 40.0 30 32.875320712849316 37.0 31.0 39.0 17.0 40.0 31 33.81409056237432 38.0 33.0 39.0 22.0 40.0 32 33.65148047985576 38.0 33.0 39.0 21.0 40.0 33 33.59226128562513 38.0 33.0 39.0 20.0 40.0 34 33.29325289508356 37.0 33.0 39.0 19.0 40.0 35 33.39907079952847 38.0 33.0 39.0 20.0 40.0 36 32.54559323209209 36.0 31.0 39.0 17.0 40.0 37 32.3227931488801 36.0 31.0 39.0 16.0 40.0 38 31.943554538520214 36.0 31.0 39.0 11.0 40.0 39 31.014215380348105 35.0 29.0 39.0 4.0 40.0 40 28.51605297829554 34.0 22.0 38.0 4.0 40.0 41 26.84682060883434 32.0 17.0 38.0 4.0 39.0 42 27.904999653283404 32.0 23.0 38.0 4.0 39.0 43 27.75431662159351 33.0 22.0 38.0 4.0 39.0 44 27.110325220165038 32.0 19.0 38.0 4.0 39.0 45 27.97198529921642 33.0 23.0 38.0 4.0 39.0 46 26.240274599542335 31.0 18.0 37.0 4.0 39.0 47 25.187157617363567 30.0 14.0 36.0 4.0 39.0 48 25.854309687261633 30.0 18.0 36.0 4.0 39.0 49 24.914569031273835 30.0 12.0 36.0 4.0 39.0 50 25.681020733652314 30.0 17.0 36.0 4.0 39.0 51 25.95222245336662 31.0 16.0 37.0 4.0 39.0 52 25.732889536093197 31.0 14.0 37.0 4.0 39.0 53 25.49226821995701 31.0 10.0 36.0 4.0 39.0 54 25.35288814922682 31.0 4.0 36.0 4.0 39.0 55 25.060398030649747 31.0 4.0 36.0 4.0 39.0 56 24.5513487275501 31.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.8950033763504948 1 2 4.942218454778676 1 3 5.157831340066529 1 4 5.495558856885594 1 5 5.497132239214245 1 6 5.135788160921251 1 7 4.3602921431330515 1 8 4.272565042593982 1 9 4.108952531007283 1 10 4.733184295980578 1 11 -1.540536080720365 1 12 -8.926923367578699 1 13 -20.485724631028464 1 14 -8.489907530108244 1 15 0.6253241446167586 1 16 -4.474826770022197 1 17 -6.518857765458478 1 18 -5.813528027555424 1 19 -1.8199651755822295 1 20 -2.6408148338041713 1 21 6.084715374151131 1 22 7.25686028732736 1 23 6.387526412339028 1 24 7.362165656206194 1 25 6.5436688649150625 1 26 5.56196526221002 1 27 6.413881478955073 1 28 4.866652917325432 1 29 6.8405091697185085 1 30 6.640162800987994 1 31 4.902741921382571 1 32 4.760891122694225 1 33 5.651921471883671 1 34 5.491599296362168 1 35 4.327599781607272 1 36 4.447683614467252 1 37 3.8659245720213384 1 38 4.90001025551539 1 39 6.023502215483671 1 40 -12.510324121086754 1 41 -4.617694326541379 1 42 -5.146552685170864 1 43 -10.3286356629382 1 44 -16.60706348929558 1 45 -6.184773814803428 1 46 -8.57547440761566 1 47 -10.226015848742213 1 48 -7.567617981342567 1 49 -14.524476946584752 1 50 -4.960728667770983 1 51 0.25060902474438507 1 52 1.1260711098084393 1 53 0.8469339571524515 1 54 0.6386048019100343 1 55 1.1697188659309568 1 56 3.0598324668397403 2 1 3.203444934792053 2 2 4.912997675557897 2 3 5.430558612793803 2 4 6.3137406750674145 2 5 6.088041330123339 2 6 5.794879070012158 2 7 4.671980454821359 2 8 4.356980627009563 2 9 4.641420063474818 2 10 4.999418062214339 2 11 2.6250483348640543 2 12 -1.0892610299163614 2 13 -0.15130904661288014 2 14 3.467884677683962 2 15 4.628570897863511 2 16 3.5154329702375406 2 17 6.435687689086976 2 18 5.225433011405613 2 19 4.865099759482703 2 20 4.2552890622997275 2 21 6.185364724800479 2 22 7.328288858755929 2 23 5.153760178572789 2 24 6.193334487375026 2 25 5.394318215564411 2 26 3.7113159115606713 2 27 5.167128232201822 2 28 5.064704865377379 2 29 5.5905091697185085 2 30 4.698604359429552 2 31 4.480663999304653 2 32 4.325826187629289 2 33 4.418155238117432 2 34 4.511079815842692 2 35 3.2237036777111676 2 36 2.6067745235581583 2 37 2.9828076889044546 2 38 2.2766336321387683 2 39 4.140385332366787 2 40 2.681234320471688 2 41 6.226461517614467 2 42 5.811239522621346 2 43 5.0512344669319305 2 44 -3.8992712815033705 2 45 6.318472938443328 2 46 4.421278839137585 2 47 1.4330750603486955 2 48 3.6564079926834054 2 49 -3.3264249985328043 2 50 3.8444661374238223 2 51 7.114245388380748 2 52 6.749447733185065 2 53 7.119661229879725 2 54 7.30418921749445 2 55 7.380757826969916 2 56 8.553338960346235 3 1 3.428600214295159 3 2 5.610202644501996 3 3 5.4740368736633656 3 4 6.618088501154368 3 5 6.392389156210292 3 6 6.0883573308817205 3 7 4.8583158585480675 3 8 4.82592472638845 3 9 5.188003914406494 3 10 5.055318683332352 3 11 4.971321626789518 3 12 4.634341454555688 3 13 5.632852444070348 3 14 4.994282193211916 3 15 -0.2363359344346243 3 16 6.5666752062623885 3 17 7.587861602130452 3 18 7.911768415132322 3 19 6.782801622836743 3 20 5.975785956709661 3 21 6.829774662688678 3 22 8.213382026457797 3 23 7.079226017082107 3 24 7.125011506008569 3 25 6.614815109974344 3 26 6.208210321498559 3 27 5.878308356425425 3 28 6.218431573451916 3 29 5.4492048218924225 3 30 7.130281378063096 3 31 4.157682632845024 3 32 5.940733019927421 3 33 5.408838467931101 3 34 5.082508387271261 3 35 5.139852746034148 3 36 7.530687567036416 3 37 7.063553030519362 3 38 7.903962824685351 3 39 6.590695891372999 3 40 9.303905127925105 3 41 11.524598163577199 3 42 10.837637038149296 3 43 11.509309001093424 3 44 10.811908842720229 3 45 10.584000888753888 3 46 11.677490019261807 3 47 12.487422886435649 3 48 12.477836564111978 3 49 12.6099104051939 3 50 12.456267379659849 3 51 11.6794627796851 3 52 11.143857671073263 3 53 10.48922644727103 3 54 11.041766857246003 3 55 11.813987640634515 3 56 10.217935233638158 4 1 2.6222761036232214 4 2 4.260400272960496 4 3 3.885104067339256 4 4 5.131922493249235 4 5 4.3153140573960656 4 6 5.317606342739431 4 7 4.133019415860325 4 8 4.363474133503075 4 9 3.3362252582800096 4 10 5.960457023253305 4 11 4.8685548283705415 4 12 5.527622086966758 4 13 5.832457187153356 4 14 5.601001560800846 4 15 4.261687780980395 4 16 6.525173229977803 4 17 8.299324052723343 4 18 9.368290154262759 4 19 7.225489369872314 4 20 6.495548802559465 4 21 5.13016991960567 4 22 6.211405741872813 4 23 6.023890048702661 4 24 6.862165656206194 4 25 4.8618506830968755 4 26 4.425601625846387 4 27 5.277517842591433 4 28 2.503016553689072 4 29 3.795054624263962 4 30 2.7765264373516345 4 31 1.2663782850189378 4 32 2.669982031785132 4 33 2.924648744610945 4 34 2.446144750907628 4 35 2.554872508879999 4 36 1.902229069012705 4 37 2.093197299294065 4 38 2.7181920736972103 4 39 2.1598658518473073 4 40 -2.0103241210867537 4 41 5.655032946185894 4 42 4.853447314829136 4 43 2.4895461552436196 4 44 6.0293001470680565 4 45 3.542498912469302 4 46 7.651798319657068 4 47 7.046711423985059 4 48 6.205109291384705 4 49 6.611886689778885 4 50 6.175634968592654 4 51 3.6142453883807484 4 52 4.444252927990259 4 53 4.210570320788815 4 54 5.184059347364581 4 55 5.487900684112773 4 56 5.87801428502156 5 1 3.2981654316864635 5 2 5.653680905371566 5 3 5.560993395402498 5 4 6.705045022893501 5 5 6.001084808384206 5 6 6.044879070012158 5 7 4.8583158585480675 5 8 4.434620378562357 5 9 4.796699566580408 5 10 5.750970857245399 5 11 6.319147713746041 5 12 6.373471889338298 5 13 6.763287226679044 5 14 5.994282193211916 5 15 7.502794500347989 5 16 6.001457814958037 5 17 9.196557254304366 5 18 9.607420589045368 5 19 7.9132364054454385 5 20 7.6714381306227075 5 21 7.351513793123459 5 22 8.039468982979532 5 23 7.340095582299497 5 24 7.690228897312913 5 25 7.701771631713477 5 26 6.512558147585519 5 27 5.965264878164557 5 28 5.957562008234525 5 29 6.36224830015329 5 30 7.173759638932658 5 31 5.375073937192852 5 32 4.7233417155796005 5 33 4.582751511409359 5 34 5.3868562133582145 5 35 4.618113615599366 5 36 6.574165827905986 5 37 4.802683465301971 5 38 6.773528042076656 5 39 5.460261108764303 5 40 9.956079040968582 5 41 11.524598163577199 5 42 9.18546312510582 5 43 10.118004653267331 5 44 9.07277840793762 5 45 9.36660958440606 5 46 11.0687943670879 5 47 11.704814190783477 5 48 10.521314824981541 5 49 10.870779970411292 5 50 9.630180423138107 5 51 10.070767127511186 5 52 9.143857671073263 5 53 11.01096557770581 5 54 10.824375552898175 5 55 10.90094416237364 5 56 10.652717842333814 6 1 3.5155567360342914 6 2 5.436289601023738 6 3 5.6914281780111935 6 4 6.44417545767611 6 5 5.34891089534073 6 6 6.4796616787078065 6 7 4.9452723802872 6 8 5.043316030736271 6 9 4.796699566580408 6 10 6.142275205071485 6 11 6.232191192006908 6 12 5.851732758903516 6 13 7.545895922331216 6 14 6.168195236690174 6 15 7.807142326434942 6 16 7.871023032349342 6 17 8.718296384739148 6 18 10.39002928469754 6 19 8.565410318488915 6 20 7.367090304535754 6 21 6.481948575732154 6 22 8.25686028732736 6 23 7.035747756212544 6 24 8.34240281035639 6 25 7.136554240409133 6 26 6.295166843237691 6 27 6.3565692259906434 6 28 6.131475051712783 6 29 6.318770039283727 6 30 6.391150943280486 6 31 5.6359435024102424 6 32 6.201602585144812 6 33 5.1479689027137105 6 34 4.343377952488652 6 35 5.357244050381976 6 36 5.443731045297291 6 37 6.7592052044324085 6 38 6.860484563815788 6 39 6.677652413112131 6 40 2.4778181714033636 6 41 11.220250337490246 6 42 9.359376168584078 6 43 9.63974378370212 6 44 9.768430581850666 6 45 9.279653062666927 6 46 10.981837845348768 6 47 12.617857669044344 6 48 12.477836564111978 6 49 11.696866926933033 6 50 11.71713694487724 6 51 11.157723649250311 6 52 11.882988105855873 6 53 10.880530795097116 6 54 11.041766857246003 6 55 11.37920503193886 6 56 10.0440221901599 7 1 2.887730649077767 7 2 5.025854818415041 7 3 4.5505586127938 7 4 5.873740675067417 7 5 5.568041330123336 7 6 4.244879070012161 7 7 4.014837597678508 7 8 3.9598377698667093 7 9 3.887134349189104 7 10 5.102275205071486 7 11 5.232191192006908 7 12 5.462167541512208 7 13 5.5615480962442625 7 14 5.286456106255393 7 15 6.167142326434941 7 16 7.079718684523257 7 17 7.455687689086979 7 18 9.108290154262761 7 19 5.763671188054133 7 20 6.821003348014013 7 21 5.781079010514766 7 22 6.456860287327363 7 23 5.896617321429936 7 24 6.62762020166074 7 25 5.540032501278695 7 26 6.26560162584639 7 27 4.055699660773254 7 28 5.637562008234525 7 29 4.76050916971851 7 30 5.8728900737152685 7 31 2.8863782850189352 7 32 3.3372547590578563 7 33 3.821012380974576 7 34 4.602508387271264 7 35 4.089417963425451 7 36 6.1567745235581555 7 37 5.771379117475885 7 38 5.750919346424482 7 39 6.388956760938218 7 40 5.216948606185973 7 41 8.975032946185895 7 42 8.28981095119277 7 43 8.184091609789075 7 44 9.949300147068058 7 45 8.217044367014754 7 46 8.402707410566155 7 47 10.143075060348696 7 48 7.557836564111977 7 49 10.12643214432434 7 50 9.590180423138108 7 51 8.914245388380746 7 52 9.455162018899351 7 53 7.899661229879726 7 54 8.67133207463731 7 55 9.027900684112772 7 56 7.736196103203376 8 1 2.367730649077771 8 2 5.005854818415045 8 3 4.780558612793804 8 4 5.0637406750674145 8 5 4.9880413301233375 8 6 5.844879070012155 8 7 4.264837597678508 8 8 4.649837769866707 8 9 3.427134349189103 8 10 3.9922752050714863 8 11 5.232191192006908 8 12 5.63216754151221 8 13 6.391548096244261 8 14 5.246456106255394 8 15 6.207142326434941 8 16 7.179718684523259 8 17 8.835687689086974 8 18 9.168290154262756 8 19 7.343671188054131 8 20 7.141003348014014 8 21 6.221079010514764 8 22 6.606860287327361 8 23 5.146617321429936 8 24 6.30762020166074 8 25 4.730032501278693 8 26 4.525601625846388 8 27 3.945699660773254 8 28 2.857562008234524 8 29 1.8405091697185085 8 30 3.462890073715265 8 31 1.316378285018935 8 32 1.8472547590578614 8 33 0.11101238097457511 8 34 1.832508387271261 8 35 1.0094179634254523 8 36 1.8067745235581611 8 37 2.661379117475885 8 38 2.9409193464244865 8 39 2.8189567609382173 8 40 -1.7330513938140264 8 41 7.655032946185894 8 42 6.53981095119277 8 43 3.9940916097890735 8 44 6.229300147068056 8 45 4.747044367014755 8 46 6.09270741056616 8 47 7.9330750603486955 8 48 6.127836564111977 8 49 7.566432144324338 8 50 7.430180423138108 8 51 6.96424538838075 8 52 7.935162018899348 8 53 7.269661229879723 8 54 7.911332074637308 8 55 7.637900684112772 8 56 6.796196103203378 9 1 2.5486830300301477 9 2 5.162997675557897 9 3 4.954368136603328 9 4 5.408978770305509 9 5 5.707088949170959 9 6 5.663926689059778 9 7 4.243409026249935 9 8 3.9045996746286136 9 9 4.355705777760534 9 10 3.142275205071485 9 11 5.565524525340244 9 12 4.601215160559832 9 13 6.812976667672832 9 14 5.217884677683962 9 15 6.616666135958752 9 16 6.0035282083327814 9 17 7.888068641467925 9 18 8.106385392358 9 19 7.900814045196988 9 20 6.445765252775917 9 21 6.744888534324289 9 22 7.304479334946411 9 23 6.677569702382314 9 24 7.098096392136931 9 25 5.418127739373936 9 26 4.949411149655912 9 27 5.286175851249443 9 28 4.7670858177583355 9 29 5.316699645908983 9 30 5.341461502286698 9 31 4.909235427876084 9 32 4.897254759057859 9 33 4.108631428593629 9 34 4.939651244414115 9 35 5.057037011044503 9 36 5.832964999748633 9 37 5.601855307952075 9 38 5.419490774995914 9 39 7.402290094271553 9 40 3.8359962252335933 9 41 9.845509136662084 9 42 9.346953808335627 9 43 10.658377324074788 9 44 9.029300147068057 9 45 8.639901509871901 9 46 11.909374077232822 9 47 13.421170298443936 9 48 10.573074659350073 9 49 13.137860715752907 9 50 10.487323280280961 9 51 12.542816816952179 9 52 9.916114399851729 9 53 11.810137420355915 9 54 11.863713027018257 9 55 10.749805446017533 9 56 9.64857705558433 10 1 2.9859124672595883 10 2 4.578582091142316 10 3 4.4760131582483496 10 4 5.541013402340141 10 5 4.769859511941519 10 6 5.272151797284884 10 7 4.451201234042138 10 8 4.4998377698667085 10 9 4.108952531007283 10 10 5.233184295980578 10 11 5.732191192006908 10 12 6.209440268784938 10 13 7.150639005335169 10 14 5.601001560800846 10 15 6.943505962798575 10 16 7.706991411795983 10 17 8.753869507268796 10 18 10.141017426990032 10 19 7.95276209714504 10 20 7.950094257104919 10 21 7.44835173778749 10 22 7.93867846914554 10 23 6.9784355032481145 10 24 7.907620201660741 10 25 7.316396137642329 10 26 5.01651071675548 10 27 4.45933602440962 10 28 4.503016553689072 10 29 6.704145533354875 10 30 5.912890073715268 10 31 5.902741921382571 10 32 6.0790729408760384 10 33 6.424648744610945 10 34 5.809781114543988 10 35 5.418508872516359 10 36 5.084047250830885 10 37 5.729560935657705 10 38 5.945464800969937 10 39 7.159865851847311 10 40 9.216948606185973 10 41 11.5641238552768 10 42 10.626174587556406 10 43 11.035000700698166 10 44 11.756572874340783 10 45 10.087953457923849 10 46 10.424525592384338 10 47 11.864893242166875 10 48 10.341472927748345 10 49 12.339159417051611 10 50 10.311998604956287 10 51 10.841518115653475 10 52 11.35334383708117 10 53 10.165115775334272 10 54 10.138604801910034 10 55 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0.7878775907711386 116 30 4.807626915820528 116 31 2.9769046008084104 116 32 3.4235705485315435 116 33 2.2715386967640505 116 34 3.029876808323891 116 35 2.64099691079387 116 36 6.2515113656634185 116 37 4.93769490694957 116 38 5.885656188529744 116 39 5.016325181990847 116 40 7.585369658817555 116 41 6.128717156712209 116 42 4.437179372245403 116 43 -0.26643470600039976 116 44 3.766142252331214 116 45 4.339149630172649 116 46 4.242707410566158 116 47 -4.553767044914462 116 48 2.319941827269872 116 49 1.092747933798023 116 50 4.524917265243371 116 51 6.272140125222855 116 52 6.377267282057243 116 53 6.251240177248146 116 54 4.858700495689938 116 55 5.09316384200751 116 56 9.222511892677062 117 1 1.7831152644623813 117 2 3.9212394337996557 117 3 3.7382509204861094 117 4 4.929125290452028 117 5 4.703425945507952 117 6 4.506417531550618 117 7 3.1994529822938915 117 8 3.3844531544820953 117 9 3.465595887650643 117 10 3.8345828973791782 117 11 0.4629604227761419 117 12 -7.248601689257018 117 13 -8.527682672986508 117 14 -2.199697739898454 117 15 -5.731319212026598 117 16 -9.212589007784437 117 17 -0.9873892339899477 117 18 -3.593248307275701 117 19 0.4282865726695171 117 20 -1.0743812673706046 117 21 3.913386702822457 117 22 6.026091056558126 117 23 4.527386552199168 117 24 3.830697124737661 117 25 4.333878655124849 117 26 3.8102170104617734 117 27 3.711084276157866 117 28 4.265254315926832 117 29 4.071278400487742 117 30 3.528274689099881 117 31 4.074070592711244 117 32 4.128023989827092 117 33 3.9071662271284247 117 34 3.7748160795789545 117 35 3.0094179634254523 117 36 5.4336976004812385 117 37 4.872917579014345 117 38 6.3755347310398705 117 39 6.992033684015137 117 40 8.678487067724433 117 41 7.578109869262818 117 42 5.182118643500466 117 43 0.4825531482506129 117 44 -0.35531523754732675 117 45 7.035505905476295 117 46 -6.295754127895382 117 47 -3.8169249396513045 117 48 -2.906778820503405 117 49 -5.356644778752585 117 50 -2.831358038400353 117 51 0.9603992345345951 117 52 1.0736235573608894 117 53 1.4658150760335715 117 54 0.9113320746373077 117 55 -0.16594546973337998 117 56 2.465426872434147 118 1 1.5010639824111038 118 2 2.889188151748378 118 3 2.2638919461271385 118 4 4.0637406750674145 118 5 3.9213746634566746 118 6 2.961545736678822 118 7 3.3148375976785047 118 8 3.083171103200044 118 9 3.177134349189103 118 10 2.142275205071485 118 11 -0.10114214132642729 118 12 -5.2678324584877885 118 13 -5.758451903755738 118 14 -10.603543893744607 118 15 -13.77619100689839 118 16 -13.520281315476744 118 17 -1.7309789775796922 118 18 -5.465043179070573 118 19 -2.2896621452791983 118 20 -3.29232998531932 118 21 1.9710790105147638 118 22 3.3401936206606955 118 23 2.0466173214299346 118 24 3.240953534994077 118 25 2.09669916794536 118 26 2.1756016258463866 118 27 1.512366327439917 118 28 0.3742286749011896 118 29 2.9238425030518442 118 30 2.4962234070486033 118 31 -1.8169550483143944 118 32 -1.2694119076088093 118 33 0.6443457143079137 118 34 0.5825083872712611 118 35 0.7594179634254523 118 36 3.6067745235581583 118 37 3.161379117475885 118 38 4.324252679757819 118 39 5.235623427604882 118 40 6.883615272852637 118 41 6.905032946185894 118 42 3.9064776178594407 118 43 0.6107582764557407 118 44 3.1126334804013887 118 45 5.663711033681423 118 46 -2.507292589433842 118 47 3.0997417270153633 118 48 1.2278365641119784 118 49 1.6497654776576702 118 50 2.796847089804775 118 51 7.614245388380748 118 52 6.785162018899349 118 53 5.536327896546393 118 54 6.8279987413039755 118 55 6.904567350779441 118 56 9.446196103203377 119 1 2.090807572154688 119 2 4.075085587645809 119 3 4.353635535870723 119 4 5.082971444298181 119 5 5.011118253200259 119 6 4.737186762319851 119 7 3.430222213063118 119 8 2.9229146929436283 119 9 3.31174973380449 119 10 4.757659820456098 119 11 -3.3062703464546317 119 12 -11.402447843103172 119 13 -6.989221134524968 119 14 -13.892005432206147 119 15 -7.115934596641981 119 16 -9.13566593086136 119 17 0.5510723044715924 119 18 -1.9778636918910877 119 19 -0.5717134273304829 119 20 -1.2282274212167579 119 21 4.99030977974553 119 22 6.026091056558126 119 23 5.142771167583781 119 24 3.9845432785838213 119 25 5.103109424355615 119 26 5.348678548923306 119 27 5.172622737696333 119 28 4.419100469772985 119 29 5.225124554333895 119 30 5.759043919869114 119 31 4.150993669634325 119 32 4.820331682134778 119 33 4.8302431502053444 119 34 4.7748160795789545 119 35 4.6248025788100655 119 36 5.895236062019698 119 37 6.026763732860498 119 38 6.29861165411679 119 39 7.761264453245911 119 40 9.293871683109053 119 41 5.578109869262818 119 42 1.3359647973466195 119 43 0.7133223790198429 119 44 1.4908386086065164 119 45 4.727813597783985 119 46 -2.2188310509723017 119 47 -3.740001862728228 119 48 -5.522163435888022 119 49 -1.7412601633679685 119 50 0.5532573462150303 119 51 6.037322311457672 119 52 5.996700480437809 119 53 4.542738152956648 119 54 1.7574859207911544 119 55 5.372516068728157 119 56 5.542349949357224 120 1 1.4754229567700747 120 2 3.152008664568889 120 3 3.584404766639956 120 4 4.390663751990495 120 5 4.241887483969492 120 6 3.4294944546275445 120 7 2.2763760592169646 120 8 2.0767608467897887 120 9 1.388672810727563 120 10 2.680736743533025 120 11 -1.1524241926084748 120 12 -4.248601689257018 120 13 -5.3738365191403545 120 14 -1.199697739898454 120 15 -5.192857673565058 120 16 -4.981819777015204 120 17 1.4741492275485157 120 18 -2.9778636918910877 120 19 0.6590558034387506 120 20 0.6179264249370888 120 21 1.836463625899377 120 22 5.026091056558126 120 23 2.527386552199168 120 24 3.446081740122281 120 25 3.5646478858940824 120 26 3.65637085661562 120 27 3.4033919684655594 120 28 3.5729466236191385 120 29 4.8405091697185085 120 30 3.9128900737152676 120 31 3.381762900403551 120 32 1.0511009129040119 120 33 1.7533200732822714 120 34 2.3132776180404946 120 35 3.394033348040839 120 36 1.8952360620196984 120 37 2.795994502091272 120 38 -0.2398498843447463 120 39 -0.007966315984859307 120 40 1.755410144647513 120 41 -6.729582438429489 120 42 -4.6640352026533805 120 43 -9.055908390210927 120 44 -18.893776776008867 120 45 -8.118340248369861 120 46 -12.06498489712615 120 47 -8.816924939651305 120 48 -5.9837018974264815 120 49 -15.048952471060277 120 50 -7.44674265378497 120 51 -2.1165238423884816 120 52 -2.849453365716034 120 53 -2.9188003085818153 120 54 -2.165591002285769 120 55 -2.550560854348767 120 56 1.157734564741837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 393.0 5 14.0 6 18.0 7 16.0 8 32.0 9 58.0 10 37.0 11 41.0 12 34.0 13 35.0 14 39.0 15 57.0 16 48.0 17 79.0 18 90.0 19 126.0 20 168.0 21 268.0 22 277.0 23 325.0 24 360.0 25 394.0 26 468.0 27 411.0 28 454.0 29 506.0 30 574.0 31 672.0 32 780.0 33 827.0 34 894.0 35 990.0 36 1107.0 37 1211.0 38 1272.0 39 1343.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 99.45218778170724 0.42299424450454204 0.09708064627973094 0.027737327508494558 8 99.52153110047847 0.40912558075029465 0.04854032313986547 0.020802995631370915 9 98.11386172942237 0.9014631440260731 0.6448928645724984 0.3397822619790583 10 44.948339227515426 35.975313778517446 11.906247833021288 7.170099160945844 11 27.3975452465155 23.909576312322308 27.792802163511542 20.900076277650648 12 28.077109770473612 22.002635046113305 25.143887386450313 24.776367796962763 13 20.629637334442826 18.02926288052146 28.02856944733375 33.312530337701965 14 17.987656889258723 31.93953262603148 27.619443866583453 22.453366618126346 15 26.02882807603927 25.617993175962678 25.959195042127988 22.393983705870067 16 24.23842897786413 34.598570536395805 23.34327943931719 17.81972104642287 17 22.939789123196448 26.914539400665927 31.437291897891235 18.708379578246394 18 21.045697247070244 26.31578947368421 25.608487622217602 27.030025657027945 19 21.096080471730836 30.218522372528618 22.282344779743323 26.403052375997227 20 22.229166666666668 30.17361111111111 30.777777777777775 16.819444444444446 21 21.21212121212121 28.167256084876225 25.164690382081687 25.455932320920876 22 17.842035919839123 29.297552180847376 33.49282296650718 19.367588932806324 23 17.446779002843076 31.27383676582761 31.81471465224326 19.464669579086056 24 16.90131077051113 32.304598099729525 24.987863236008046 25.8062278937513 25 18.9792663476874 36.16254073919978 28.423826364329795 16.434366548783025 26 26.551006245662734 35.759888965995835 20.25676613462873 17.4323386537127 27 17.780553820528837 38.30938996460546 21.95155805399403 21.958498160871677 28 22.1497919556172 35.67267683772538 22.219140083217752 19.958391123439668 29 22.534037232564604 36.61433731592109 21.360100027785496 19.491525423728813 30 22.212966819380814 31.577120644176038 27.571845064556435 18.638067471886714 31 23.684028018586588 36.13981552118732 22.87259865455302 17.30355780567307 32 24.894251438873866 35.33042091394494 22.37015463560086 17.405173011580334 33 25.942904771827465 34.82670000694589 21.768423977217477 17.46197124400917 34 19.417812977629566 31.957760177851885 31.249131582603862 17.375295261914687 35 17.89312764922521 34.84816899451046 31.71426586060732 15.544437495657007 36 20.409295872355184 34.935830731876514 26.576482830385018 18.07839056538328 37 22.93157346300799 34.24800277874262 25.314345258770405 17.506078499478985 38 23.20795383438782 33.782938190919836 26.176736424946117 16.832371549746227 39 22.744008336227857 34.19242792636332 21.431052448766934 21.63251128864189 40 18.49933505984461 27.465528102470778 24.05683488486036 29.978301952824243 41 15.771741419246158 24.075243910998807 20.923703235768933 39.229311433986105 42 17.89341867260331 37.22693752608877 22.331988312230415 22.5476554890775 43 15.950792326939117 32.84681679177092 25.18765638031693 26.014734500973034 44 16.637660981552386 34.368256178210935 24.775495997215454 24.21858684302123 45 20.385176945004517 33.351873739831746 26.698185357713967 19.564763957449767 46 17.767732962447845 37.037552155771905 22.969401947148818 22.225312934631432 47 14.945330454767044 32.167978271467376 21.53353297583397 31.35315829793161 48 20.75209879969472 34.205231388329985 23.895094706168045 21.147575105807256 49 17.19944405837387 34.15566365531619 22.793606671299514 25.851285615010422 50 17.23299284274894 36.22402890695574 24.126189979848515 22.41678827044681 51 17.78086956521739 35.568695652173915 24.04869565217391 22.601739130434783 52 17.179576241750606 39.39562348037513 25.00173671413685 18.42306356373741 53 17.38434782608696 38.9704347826087 24.577391304347827 19.067826086956522 54 17.29553192967827 34.702244458342015 28.43443819053575 19.567785421443958 55 16.808066759388037 33.39360222531293 27.628650904033382 22.169680111265645 56 16.54145798553144 34.34195882025598 28.01196438508626 21.104618809126322 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 13.5 11 27.0 12 96.0 13 203.5 14 242.0 15 262.5 16 283.0 17 256.0 18 229.0 19 214.5 20 200.0 21 188.5 22 183.0 23 189.0 24 178.5 25 168.0 26 174.5 27 181.0 28 200.0 29 228.5 30 238.0 31 253.0 32 268.0 33 279.0 34 290.0 35 324.5 36 359.0 37 373.5 38 380.0 39 372.0 40 416.5 41 461.0 42 490.0 43 519.0 44 642.5 45 766.0 46 915.0 47 1034.0 48 1004.0 49 912.0 50 820.0 51 794.5 52 769.0 53 759.5 54 734.5 55 719.0 56 666.5 57 614.0 58 612.5 59 611.0 60 633.0 61 655.0 62 596.0 63 473.0 64 409.0 65 340.0 66 271.0 67 220.5 68 170.0 69 148.0 70 126.0 71 96.5 72 61.5 73 56.0 74 33.5 75 11.0 76 10.5 77 10.0 78 8.5 79 7.0 80 7.0 81 5.0 82 3.0 83 1.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.41605991262741837 16 0.0693433187712364 17 0.0346716593856182 18 0.0 19 0.04160599126274183 20 0.1456209694195964 21 0.0 22 0.0 23 0.0 24 0.013868663754247279 25 0.0 26 0.07627765064836003 27 0.08321198252548366 28 0.0069343318771236395 29 0.173358296928091 30 0.10401497815685459 31 0.013868663754247279 32 0.0 33 0.16642396505096732 34 0.18722696068233824 35 0.20802995631370919 36 0.04160599126274183 37 0.18029262880521463 38 0.2635046113306983 39 0.18029262880521463 40 0.9292004715345676 41 1.2065737466195132 42 0.32591359822481103 43 0.2288329519450801 44 0.38832258511892376 45 0.2635046113306983 46 0.2843076069620692 47 0.42992857638166565 48 0.055474655016989116 49 0.21496428819083283 50 0.20802995631370919 51 0.3189792663476874 52 0.18029262880521463 53 0.3189792663476874 54 0.20802995631370919 55 0.2843076069620692 56 0.31204493447056375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 14421.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.21343873517787 #Duplication Level Percentage of deduplicated Percentage of total 1 89.92071524966262 73.92691214201513 2 7.101889338731445 11.677414881076208 3 1.4760458839406208 3.6405242354899108 4 0.5735492577597842 1.8861382705776297 5 0.2614709851551957 1.074821440954164 6 0.19399460188933873 0.9569377990430622 7 0.06747638326585695 0.38832258511892376 8 0.07591093117408906 0.499271895152902 9 0.05060728744939271 0.3744539213646765 >10 0.2614709851551957 3.827751196172249 >50 0.0 0.0 >100 0.016869095816464237 1.747451633035157 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CACAAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 151 1.0470841134456697 No Hit CACAAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 101 0.7003675195894876 No Hit CACAAGGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 39 0.2704389432078219 No Hit CACAAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 36 0.249635947576451 No Hit CACAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 34 0.23576728382220372 No Hit CACAAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 32 0.22189862006795646 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 29 0.20109562443658552 No Hit CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 27 0.18722696068233824 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 27 0.18722696068233824 No Hit CACAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 27 0.18722696068233824 No Hit CACAAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 20 0.1386866375424728 No Hit CACAAGGGGATTCGTAGGAGTAAACATAACATTCTTCCCTCAACATTTCCTGGGCC 19 0.13175230566534915 No Hit CACAAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATCATTATACTATTATAT 18 0.1248179737882255 No Hit CACAAGGGGGTGTACTCTGTATTATCGTTCTGATGCCAAATGAAGATACAACTGAA 18 0.1248179737882255 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 16 0.11094931003397823 No Hit CACAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.10401497815685459 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0069343318771236395 0.0 0.0 0.0 0.0 6 0.0069343318771236395 0.0 0.0 0.0 0.0 7 0.0069343318771236395 0.0 0.0 0.0 0.0 8 0.0069343318771236395 0.0 0.0 0.0 0.0 9 0.0069343318771236395 0.0 0.0 0.0 0.0 10 0.0069343318771236395 0.0 0.0 0.0 0.0 11 0.0069343318771236395 0.0 0.0 0.0 0.0 12 0.0069343318771236395 0.0 0.0 0.0 0.0 13 0.02080299563137092 0.0 0.0 0.0 0.0 14 0.0346716593856182 0.0 0.0 0.0 0.0 15 0.0346716593856182 0.0 0.0 0.0 0.0 16 0.0346716593856182 0.0 0.0 0.0 0.0 17 0.0346716593856182 0.0 0.0 0.0 0.0 18 0.0346716593856182 0.0 0.0 0.0 0.0 19 0.0346716593856182 0.0 0.0 0.0 0.0 20 0.0346716593856182 0.0 0.0 0.0 0.0 21 0.0346716593856182 0.0 0.0 0.0 0.0 22 0.0346716593856182 0.0 0.0 0.0 0.0 23 0.04160599126274184 0.0 0.0 0.0 0.0 24 0.04854032313986548 0.0 0.0 0.0 0.0 25 0.055474655016989116 0.0 0.0 0.0 0.0 26 0.055474655016989116 0.0 0.0 0.0 0.0 27 0.055474655016989116 0.0 0.0 0.0 0.0 28 0.055474655016989116 0.0 0.0 0.0 0.0 29 0.0693433187712364 0.0 0.0 0.0 0.0 30 0.0693433187712364 0.0 0.0 0.0 0.0 31 0.08321198252548367 0.0 0.0 0.0 0.0 32 0.09708064627973095 0.0 0.0 0.0 0.0 33 0.09708064627973095 0.0 0.0 0.0 0.0 34 0.09708064627973095 0.0 0.0 0.0069343318771236395 0.0 35 0.10401497815685459 0.0 0.0 0.0069343318771236395 0.0 36 0.10401497815685459 0.0 0.0 0.0069343318771236395 0.0 37 0.10401497815685459 0.0 0.0 0.0069343318771236395 0.0 38 0.10401497815685459 0.0 0.0 0.0069343318771236395 0.0 39 0.10401497815685459 0.0 0.0 0.0069343318771236395 0.0 40 0.10401497815685459 0.0 0.0 0.0069343318771236395 0.0 41 0.10401497815685459 0.0 0.0 0.0069343318771236395 0.0 42 0.11094931003397823 0.0 0.0 0.0069343318771236395 0.0 43 0.12481797378822551 0.0 0.0 0.0069343318771236395 0.0 44 0.14562096941959643 0.0 0.0 0.0069343318771236395 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGACT 15 0.0075252936 51.189964 41 CGGATTG 40 1.9226718E-9 51.189964 39 GCGGATT 40 1.9226718E-9 51.189964 38 AACCAAC 15 0.0075252936 51.189964 41 GCGCATT 15 0.0075252936 51.189964 38 ACAACAA 15 0.0075252936 51.189964 39 GGCGCAT 15 0.007633075 51.00714 37 GGCGGAT 40 1.9863364E-9 51.00714 37 AAATTAC 15 0.0077420017 50.825623 34 TTGGGCG 65 0.0 50.825623 34 TCAAACC 15 0.0077420017 50.825623 35 ATGCTTG 15 0.0077420017 50.825623 36 TGGGCGC 15 0.0077420017 50.825623 35 GGGCGCA 15 0.0077420017 50.825623 36 TGACTGC 15 0.007963319 50.46643 43 GTGGTAT 20 4.070981E-4 49.93706 15 GGTTGTT 15 0.008420036 49.76307 9 TATCAAC 30 1.0092663E-6 49.76307 19 TCCCCGC 15 0.008420036 49.76307 10 TCACAGA 80 0.0 49.763065 10 >>END_MODULE