FastQCFastQC Report
Fri 17 Jun 2016
SRR1526141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1197
Sequences flagged as poor quality0
Sequence length40
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGGGGAGGCAGGAAAAATACTGGTGTATTACAAGGA40.3341687552213868No Hit
CACAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT30.2506265664160401No Hit
CACAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT30.2506265664160401No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC30.2506265664160401No Hit
CACAAGGGGAATCAACGCAGAGTCGACTTTTTTTTTTTTT20.1670843776106934No Hit
CACAAGGGGAGCAGAGGTATAACACCTCACATAGAGAAAT20.1670843776106934No Hit
CACAAGGGGAGAGCCCGGGCTTCTACAGGAACGTGGTGAA20.1670843776106934No Hit
CACAAGGGGGCCGAATAGCCTCTCCACCCCAGCGGCCCGA20.1670843776106934No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTCAGAGC20.1670843776106934No Hit
CACAAGGGGTACAACGCAGGGTCGATTTTTTTTTTTTTTT20.1670843776106934No Hit
CACAAGGGGAATCAGTCGCCTTTTTTTTTTTTTTTTTTTT20.1670843776106934No Hit
CACAAGGGGATCCAGTGACAGATACCATTAGATTCAAGAG20.1670843776106934No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGC20.1670843776106934No Hit
CACAAGGGGAAGTATTGTTATGTTATACCCGGCAGTGAGT20.1670843776106934No Hit
CACAAGGGGAAGCATGTATAACGCAGAGTCGACTTTTTTT20.1670843776106934No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG20.1670843776106934No Hit
CACAAGGGGGTAGCATCATCTTCTATAAAGAACCCAGGAG20.1670843776106934No Hit
CACAAGGGGAGTCACAACCCTTTCACTGTTTGTCATTTCT20.1670843776106934No Hit
CACAAGGGGATAGTTCATGTTAATAGTGGGGCTTTCCGGT20.1670843776106934No Hit
CACAAGGGGAAGGCTACATTTGCTTGCTTTCTGCTGGCCC20.1670843776106934No Hit
CACAAGGGGAAGGCTACATTTGCTTGCTTTCTGCTGGCCG20.1670843776106934No Hit
CACAAGGGGTTTTGTAAAGATGTATAAGAGTAAGAAAACT20.1670843776106934No Hit
CACAAGGGGGAAAAAAAACAAAAAAAAAAAAAAAAAAAAA20.1670843776106934No Hit
CACAAGGGGGTTTTTGTTCTTGTGTGTGTGTGTTTGTGTT20.1670843776106934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGA456.657501E-1034.0000044
ACAAGGG1100.034.0000042
CAAGGGG1100.034.0000043
AGGGGGG200.001787722834.05
CACAAGG1150.034.01
AAGGGGG401.4437319E-834.04
AAGGGGC200.001787722834.04
Produced by FastQC (version 0.11.5)