##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526141_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1197 Sequences flagged as poor quality 0 Sequence length 40 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.263993316624894 29.0 4.0 32.0 4.0 33.0 2 10.102756892230577 4.0 4.0 14.0 4.0 26.0 3 11.435254803675857 4.0 4.0 19.0 4.0 29.0 4 11.167919799498748 4.0 4.0 17.0 4.0 29.0 5 9.312447786131997 4.0 4.0 11.0 4.0 24.0 6 11.604010025062657 4.0 4.0 18.0 4.0 30.0 7 12.475355054302423 4.0 4.0 22.0 4.0 30.0 8 11.283208020050125 4.0 4.0 18.0 4.0 28.0 9 11.967418546365915 4.0 4.0 20.0 4.0 30.0 10 9.909774436090226 4.0 4.0 14.0 4.0 26.0 11 13.000835421888054 4.0 4.0 26.0 4.0 32.0 12 13.53717627401838 4.0 4.0 27.0 4.0 33.0 13 13.385129490392648 4.0 4.0 27.0 4.0 33.0 14 13.543024227234753 4.0 4.0 28.0 4.0 33.0 15 13.142021720969089 4.0 4.0 28.0 4.0 33.0 16 11.238930659983291 4.0 4.0 20.0 4.0 31.0 17 11.092731829573935 4.0 4.0 20.0 4.0 31.0 18 10.928153717627401 4.0 4.0 19.0 4.0 31.0 19 10.216374269005849 4.0 4.0 15.0 4.0 30.0 20 10.485380116959064 4.0 4.0 17.0 4.0 31.0 21 9.260651629072681 4.0 4.0 7.0 4.0 29.0 22 9.160401002506266 4.0 4.0 4.0 4.0 30.0 23 8.885547201336674 4.0 4.0 4.0 4.0 29.0 24 8.730994152046783 4.0 4.0 4.0 4.0 29.0 25 8.155388471177945 4.0 4.0 4.0 4.0 27.0 26 7.363408521303258 4.0 4.0 4.0 4.0 22.0 27 7.405179615705931 4.0 4.0 4.0 4.0 23.0 28 7.343358395989975 4.0 4.0 4.0 4.0 24.0 29 6.909774436090226 4.0 4.0 4.0 4.0 20.0 30 6.530492898913952 4.0 4.0 4.0 4.0 15.0 31 6.608187134502924 4.0 4.0 4.0 4.0 17.0 32 6.32999164578112 4.0 4.0 4.0 4.0 12.0 33 6.504594820384294 4.0 4.0 4.0 4.0 13.0 34 6.326649958228906 4.0 4.0 4.0 4.0 4.0 35 6.170426065162907 4.0 4.0 4.0 4.0 4.0 36 6.442773600668337 4.0 4.0 4.0 4.0 4.0 37 6.314118629908104 4.0 4.0 4.0 4.0 4.0 38 6.364243943191312 4.0 4.0 4.0 4.0 4.0 39 6.358395989974937 4.0 4.0 4.0 4.0 4.0 40 6.3274853801169595 4.0 4.0 4.0 4.0 4.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 14.659953703703703 1 2 10.140046296296298 1 3 -0.8421296296296301 1 4 12.572685185185186 1 5 13.087499999999999 1 6 13.193287037037036 1 7 11.660185185185185 1 8 6.422453703703702 1 9 10.718287037037037 1 10 12.117361111111112 1 11 5.721527777777778 1 12 -1.6319444444444464 1 13 -5.047685185185186 1 14 4.80648148148148 1 15 -0.5645833333333332 1 16 6.094444444444443 1 17 2.132638888888888 1 18 2.976157407407408 1 19 -3.408564814814815 1 20 6.273148148148147 1 21 12.935648148148148 1 22 15.756944444444445 1 23 10.760416666666668 1 24 12.62662037037037 1 25 14.952546296296298 1 26 9.218518518518518 1 27 18.809953703703705 1 28 18.455092592592592 1 29 15.366435185185185 1 30 14.966898148148148 1 31 19.04513888888889 1 32 16.794212962962963 1 33 21.01273148148148 1 34 19.44421296296296 1 35 16.95601851851852 1 36 11.30162037037037 1 37 7.217592592592593 1 38 9.333564814814816 1 39 10.203703703703704 1 40 10.53425925925926 6 1 -13.590046296296297 6 2 -4.609953703703702 6 3 -5.59212962962963 6 4 -5.177314814814814 6 5 -4.162500000000001 6 6 -5.806712962962964 6 7 -7.089814814814815 6 8 -5.827546296296298 6 9 -6.531712962962963 6 10 -4.882638888888888 6 11 -7.528472222222222 6 12 -8.131944444444446 6 13 -8.797685185185186 6 14 -8.44351851851852 6 15 -7.814583333333333 6 16 -6.655555555555557 6 17 -6.117361111111112 6 18 -5.773842592592592 6 19 -5.408564814814815 6 20 -5.476851851851853 6 21 -4.0643518518518515 6 22 -3.7430555555555554 6 23 -3.739583333333333 6 24 -4.12337962962963 6 25 -3.7974537037037033 6 26 -2.2814814814814817 6 27 -2.9400462962962965 6 28 -2.0449074074074067 6 29 -1.8835648148148145 6 30 -1.2831018518518515 6 31 -1.4548611111111107 6 32 -1.2057870370370374 6 33 -1.4872685185185182 6 34 -1.555787037037038 6 35 -1.2939814814814818 6 36 -1.9483796296296294 6 37 -1.7824074074074074 6 38 -1.6664351851851844 6 39 -2.046296296296296 6 40 -1.9657407407407401 7 1 -13.590046296296297 7 2 -4.609953703703702 7 3 -5.59212962962963 7 4 -5.177314814814814 7 5 -4.162500000000001 7 6 -5.806712962962964 7 7 -7.089814814814815 7 8 -5.827546296296298 7 9 -6.531712962962963 7 10 -4.882638888888888 7 11 -7.528472222222222 7 12 -8.131944444444446 7 13 -8.797685185185186 7 14 -8.44351851851852 7 15 -7.814583333333333 7 16 -6.655555555555557 7 17 -6.117361111111112 7 18 -5.773842592592592 7 19 -5.408564814814815 7 20 -5.476851851851853 7 21 -4.0643518518518515 7 22 -3.7430555555555554 7 23 -3.739583333333333 7 24 -4.12337962962963 7 25 -3.7974537037037033 7 26 -2.2814814814814817 7 27 -2.9400462962962965 7 28 -2.0449074074074067 7 29 -1.8835648148148145 7 30 -1.2831018518518515 7 31 -1.4548611111111107 7 32 -1.2057870370370374 7 33 -1.4872685185185182 7 34 -1.555787037037038 7 35 -1.2939814814814818 7 36 -1.9483796296296294 7 37 -1.7824074074074074 7 38 -1.6664351851851844 7 39 -2.046296296296296 7 40 -1.9657407407407401 10 1 14.159953703703703 10 2 -2.1099537037037024 10 3 3.40787037037037 10 4 -0.17731481481481381 10 5 -1.4125000000000014 10 6 2.9432870370370363 10 7 2.6601851851851848 10 8 1.6724537037037024 10 9 2.7182870370370367 10 10 1.3673611111111121 10 11 5.721527777777778 10 12 4.618055555555554 10 13 2.952314814814814 10 14 -2.1935185185185198 10 15 3.185416666666667 10 16 0.09444444444444322 10 17 5.882638888888888 10 18 0.22615740740740797 10 19 0.5914351851851851 10 20 -1.476851851851853 10 21 2.6856481481481485 10 22 3.0069444444444446 10 23 -3.739583333333333 10 24 -4.12337962962963 10 25 -3.7974537037037033 10 26 -2.2814814814814817 10 27 -2.9400462962962965 10 28 -2.0449074074074067 10 29 -1.8835648148148145 10 30 -1.2831018518518515 10 31 -1.4548611111111107 10 32 -1.2057870370370374 10 33 -1.4872685185185182 10 34 -1.555787037037038 10 35 -1.2939814814814818 10 36 -1.9483796296296294 10 37 -1.7824074074074074 10 38 -1.6664351851851844 10 39 -2.046296296296296 10 40 -1.9657407407407401 12 1 9.409953703703703 12 2 -0.6099537037037024 12 3 0.4078703703703699 12 4 -4.177314814814814 12 5 -1.1625000000000014 12 6 -0.8067129629629637 12 7 2.9101851851851848 12 8 0.1724537037037024 12 9 -4.531712962962963 12 10 2.117361111111112 12 11 21.47152777777778 12 12 17.868055555555554 12 13 17.202314814814812 12 14 20.55648148148148 12 15 18.18541666666667 12 16 17.34444444444444 12 17 9.882638888888888 12 18 14.226157407407408 12 19 14.591435185185185 12 20 22.523148148148145 12 21 -4.0643518518518515 12 22 -3.7430555555555554 12 23 -3.739583333333333 12 24 -4.12337962962963 12 25 -3.7974537037037033 12 26 -2.2814814814814817 12 27 -2.9400462962962965 12 28 -2.0449074074074067 12 29 -1.8835648148148145 12 30 -1.2831018518518515 12 31 -1.4548611111111107 12 32 -1.2057870370370374 12 33 -1.4872685185185182 12 34 -1.555787037037038 12 35 -1.2939814814814818 12 36 -1.9483796296296294 12 37 -1.7824074074074074 12 38 -1.6664351851851844 12 39 -2.046296296296296 12 40 -1.9657407407407401 16 1 -13.590046296296297 16 2 -4.609953703703702 16 3 -5.59212962962963 16 4 -5.177314814814814 16 5 -4.162500000000001 16 6 -5.806712962962964 16 7 -7.089814814814815 16 8 -5.827546296296298 16 9 -6.531712962962963 16 10 -4.882638888888888 16 11 -7.528472222222222 16 12 -8.131944444444446 16 13 -8.797685185185186 16 14 -8.44351851851852 16 15 -7.814583333333333 16 16 -6.655555555555557 16 17 -6.117361111111112 16 18 -5.773842592592592 16 19 -5.408564814814815 16 20 -5.476851851851853 16 21 -4.0643518518518515 16 22 -3.7430555555555554 16 23 -3.739583333333333 16 24 -4.12337962962963 16 25 -3.7974537037037033 16 26 -2.2814814814814817 16 27 -2.9400462962962965 16 28 -2.0449074074074067 16 29 -1.8835648148148145 16 30 -1.2831018518518515 16 31 -1.4548611111111107 16 32 -1.2057870370370374 16 33 -1.4872685185185182 16 34 -1.555787037037038 16 35 -1.2939814814814818 16 36 -1.9483796296296294 16 37 -1.7824074074074074 16 38 -1.6664351851851844 16 39 -2.046296296296296 16 40 -1.9657407407407401 17 1 7.409953703703703 17 2 3.3900462962962976 17 3 3.2078703703703706 17 4 3.822685185185186 17 5 0.23749999999999893 17 6 5.393287037037036 17 7 4.7101851851851855 17 8 3.772453703703702 17 9 4.468287037037037 17 10 1.5173611111111125 17 11 0.4715277777777782 17 12 1.4680555555555532 17 13 1.0023148148148149 17 14 0.7564814814814795 17 15 0.18541666666666679 17 16 3.7444444444444436 17 17 1.4826388888888875 17 18 3.226157407407408 17 19 0.7914351851851844 17 20 2.7231481481481463 17 21 1.7356481481481492 17 22 3.656944444444445 17 23 3.8604166666666666 17 24 5.676620370370371 17 25 4.402546296296296 17 26 -0.48148148148148184 17 27 1.8599537037037042 17 28 2.3550925925925936 17 29 1.5164351851851858 17 30 2.116898148148149 17 31 3.7451388888888886 17 32 3.3942129629629623 17 33 3.3127314814814826 17 34 3.8442129629629624 17 35 4.1060185185185185 17 36 4.25162037037037 17 37 4.417592592592592 17 38 4.533564814814815 17 39 3.553703703703704 17 40 4.234259259259259 18 1 2.209953703703704 18 2 -3.6099537037037024 18 3 -3.7921296296296303 18 4 -3.377314814814814 18 5 -3.1625000000000014 18 6 -4.806712962962964 18 7 -4.489814814814816 18 8 -4.827546296296298 18 9 -3.9317129629629637 18 10 -2.882638888888888 18 11 -4.328472222222222 18 12 -2.931944444444447 18 13 -3.5976851851851865 18 14 -3.0435185185185194 18 15 -2.614583333333334 18 16 -3.055555555555557 18 17 -1.1173611111111121 18 18 -0.773842592592592 18 19 0.5914351851851851 18 20 0.12314814814814667 18 21 0.9356481481481485 18 22 -1.5430555555555552 18 23 0.6604166666666673 18 24 -2.7233796296296298 18 25 -1.1974537037037036 18 26 2.9185185185185176 18 27 2.459953703703704 18 28 0.5550925925925929 18 29 3.916435185185186 18 30 -1.2831018518518515 18 31 -1.4548611111111107 18 32 -1.2057870370370374 18 33 -1.4872685185185182 18 34 -1.555787037037038 18 35 -1.2939814814814818 18 36 -1.9483796296296294 18 37 -1.7824074074074074 18 38 -1.6664351851851844 18 39 -2.046296296296296 18 40 -1.9657407407407401 19 1 15.409953703703703 19 2 -4.609953703703702 19 3 -5.59212962962963 19 4 -5.177314814814814 19 5 -4.162500000000001 19 6 -5.806712962962964 19 7 -7.089814814814815 19 8 -5.827546296296298 19 9 -6.531712962962963 19 10 -4.882638888888888 19 11 -7.528472222222222 19 12 -8.131944444444446 19 13 -8.797685185185186 19 14 -8.44351851851852 19 15 -7.814583333333333 19 16 -6.655555555555557 19 17 -6.117361111111112 19 18 -5.773842592592592 19 19 -5.408564814814815 19 20 -5.476851851851853 19 21 -4.0643518518518515 19 22 -3.7430555555555554 19 23 -3.739583333333333 19 24 -4.12337962962963 19 25 -3.7974537037037033 19 26 -2.2814814814814817 19 27 -2.9400462962962965 19 28 -2.0449074074074067 19 29 -1.8835648148148145 19 30 -1.2831018518518515 19 31 -1.4548611111111107 19 32 -1.2057870370370374 19 33 -1.4872685185185182 19 34 -1.555787037037038 19 35 -1.2939814814814818 19 36 -1.9483796296296294 19 37 -1.7824074074074074 19 38 -1.6664351851851844 19 39 -2.046296296296296 19 40 -1.9657407407407401 22 1 -13.590046296296297 22 2 -4.609953703703702 22 3 -5.59212962962963 22 4 -5.177314814814814 22 5 -4.162500000000001 22 6 -5.806712962962964 22 7 -7.089814814814815 22 8 -5.827546296296298 22 9 -6.531712962962963 22 10 -4.882638888888888 22 11 -7.528472222222222 22 12 -8.131944444444446 22 13 -8.797685185185186 22 14 -8.44351851851852 22 15 -7.814583333333333 22 16 -6.655555555555557 22 17 -6.117361111111112 22 18 -5.773842592592592 22 19 -5.408564814814815 22 20 -5.476851851851853 22 21 -4.0643518518518515 22 22 -3.7430555555555554 22 23 -3.739583333333333 22 24 -4.12337962962963 22 25 -3.7974537037037033 22 26 -2.2814814814814817 22 27 -2.9400462962962965 22 28 -2.0449074074074067 22 29 -1.8835648148148145 22 30 -1.2831018518518515 22 31 -1.4548611111111107 22 32 -1.2057870370370374 22 33 -1.4872685185185182 22 34 -1.555787037037038 22 35 -1.2939814814814818 22 36 -1.9483796296296294 22 37 -1.7824074074074074 22 38 -1.6664351851851844 22 39 -2.046296296296296 22 40 -1.9657407407407401 24 1 -0.5900462962962969 24 2 -1.6099537037037024 24 3 3.40787037037037 24 4 -0.17731481481481381 24 5 -1.6625000000000014 24 6 -0.8067129629629637 24 7 -0.08981481481481524 24 8 -0.3275462962962976 24 9 -1.0317129629629633 24 10 0.11736111111111214 24 11 6.471527777777778 24 12 1.8680555555555536 24 13 5.202314814814814 24 14 5.55648148148148 24 15 6.185416666666667 24 16 7.344444444444443 24 17 6.882638888888888 24 18 8.226157407407408 24 19 5.091435185185185 24 20 8.023148148148147 24 21 1.9356481481481485 24 22 5.256944444444445 24 23 5.760416666666667 24 24 7.87662037037037 24 25 9.202546296296298 24 26 -2.2814814814814817 24 27 -2.9400462962962965 24 28 -2.0449074074074067 24 29 -1.8835648148148145 24 30 -1.2831018518518515 24 31 -1.4548611111111107 24 32 -1.2057870370370374 24 33 -1.4872685185185182 24 34 -1.555787037037038 24 35 -1.2939814814814818 24 36 -1.9483796296296294 24 37 -1.7824074074074074 24 38 -1.6664351851851844 24 39 -2.046296296296296 24 40 -1.9657407407407401 25 1 19.009953703703705 25 2 23.390046296296298 25 3 24.60787037037037 25 4 26.622685185185183 25 5 16.6375 25 6 24.993287037037035 25 7 23.310185185185183 25 8 26.5724537037037 25 9 24.068287037037038 25 10 24.51736111111111 25 11 17.07152777777778 25 12 19.068055555555553 25 13 19.802314814814814 25 14 12.55648148148148 25 15 21.38541666666667 25 16 17.544444444444444 25 17 8.48263888888889 25 18 20.02615740740741 25 19 18.391435185185188 25 20 19.723148148148148 25 21 16.935648148148147 25 22 24.65694444444444 25 23 26.260416666666668 25 24 22.47662037037037 25 25 13.402546296296297 25 26 13.31851851851852 25 27 14.459953703703702 25 28 10.355092592592591 25 29 10.116435185185185 25 30 4.116898148148149 25 31 5.34513888888889 25 32 7.594212962962963 25 33 7.712731481481481 25 34 4.644212962962961 25 35 3.306018518518518 25 36 7.051620370370371 25 37 7.017592592592593 25 38 7.533564814814815 25 39 7.3537037037037045 25 40 9.03425925925926 26 1 -0.5900462962962969 26 2 -0.6099537037037024 26 3 -4.09212962962963 26 4 3.822685185185186 26 5 0.3374999999999986 26 6 3.6932870370370363 26 7 1.9101851851851848 26 8 1.6724537037037024 26 9 2.9682870370370367 26 10 4.617361111111112 26 11 2.971527777777778 26 12 7.868055555555554 26 13 7.202314814814814 26 14 6.55648148148148 26 15 8.185416666666667 26 16 2.344444444444443 26 17 4.382638888888888 26 18 3.726157407407408 26 19 5.091435185185185 26 20 5.023148148148147 26 21 10.935648148148148 26 22 -3.7430555555555554 26 23 -3.739583333333333 26 24 -4.12337962962963 26 25 -3.7974537037037033 26 26 -2.2814814814814817 26 27 -2.9400462962962965 26 28 -2.0449074074074067 26 29 -1.8835648148148145 26 30 -1.2831018518518515 26 31 -1.4548611111111107 26 32 -1.2057870370370374 26 33 -1.4872685185185182 26 34 -1.555787037037038 26 35 -1.2939814814814818 26 36 -1.9483796296296294 26 37 -1.7824074074074074 26 38 -1.6664351851851844 26 39 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-2.2814814814814817 102 27 -2.9400462962962965 102 28 -2.0449074074074067 102 29 -1.8835648148148145 102 30 -1.2831018518518515 102 31 -1.4548611111111107 102 32 -1.2057870370370374 102 33 -1.4872685185185182 102 34 -1.555787037037038 102 35 -1.2939814814814818 102 36 -1.9483796296296294 102 37 -1.7824074074074074 102 38 -1.6664351851851844 102 39 -2.046296296296296 102 40 -1.9657407407407401 104 1 13.909953703703703 104 2 4.890046296296298 104 3 8.90787037037037 104 4 6.822685185185186 104 5 5.837499999999999 104 6 9.193287037037036 104 7 6.910185185185185 104 8 7.172453703703702 104 9 5.968287037037037 104 10 3.617361111111112 104 11 7.471527777777778 104 12 1.3680555555555536 104 13 3.202314814814814 104 14 4.55648148148148 104 15 7.185416666666667 104 16 4.344444444444443 104 17 7.382638888888888 104 18 7.226157407407408 104 19 -5.408564814814815 104 20 -5.476851851851853 104 21 -4.0643518518518515 104 22 -3.7430555555555554 104 23 -3.739583333333333 104 24 -4.12337962962963 104 25 -3.7974537037037033 104 26 -2.2814814814814817 104 27 -2.9400462962962965 104 28 -2.0449074074074067 104 29 -1.8835648148148145 104 30 -1.2831018518518515 104 31 -1.4548611111111107 104 32 -1.2057870370370374 104 33 -1.4872685185185182 104 34 -1.555787037037038 104 35 -1.2939814814814818 104 36 -1.9483796296296294 104 37 -1.7824074074074074 104 38 -1.6664351851851844 104 39 -2.046296296296296 104 40 -1.9657407407407401 105 1 -13.590046296296297 105 2 -4.609953703703702 105 3 -5.59212962962963 105 4 -5.177314814814814 105 5 -4.162500000000001 105 6 -5.806712962962964 105 7 -7.089814814814815 105 8 -5.827546296296298 105 9 -6.531712962962963 105 10 -4.882638888888888 105 11 -7.528472222222222 105 12 -8.131944444444446 105 13 -8.797685185185186 105 14 -8.44351851851852 105 15 -7.814583333333333 105 16 -6.655555555555557 105 17 -6.117361111111112 105 18 -5.773842592592592 105 19 -5.408564814814815 105 20 -5.476851851851853 105 21 -4.0643518518518515 105 22 -3.7430555555555554 105 23 -3.739583333333333 105 24 -4.12337962962963 105 25 -3.7974537037037033 105 26 -2.2814814814814817 105 27 -2.9400462962962965 105 28 -2.0449074074074067 105 29 -1.8835648148148145 105 30 -1.2831018518518515 105 31 -1.4548611111111107 105 32 -1.2057870370370374 105 33 -1.4872685185185182 105 34 -1.555787037037038 105 35 -1.2939814814814818 105 36 -1.9483796296296294 105 37 -1.7824074074074074 105 38 -1.6664351851851844 105 39 -2.046296296296296 105 40 -1.9657407407407401 106 1 7.909953703703703 106 2 3.8900462962962976 106 3 4.90787037037037 106 4 -0.6773148148148138 106 5 2.3374999999999986 106 6 5.193287037037036 106 7 5.910185185185185 106 8 8.172453703703702 106 9 7.468287037037037 106 10 1.6173611111111121 106 11 23.47152777777778 106 12 21.368055555555554 106 13 20.702314814814812 106 14 19.05648148148148 106 15 23.68541666666667 106 16 1.3444444444444432 106 17 -0.6173611111111121 106 18 -1.773842592592592 106 19 8.091435185185185 106 20 9.023148148148147 106 21 -4.0643518518518515 106 22 -3.7430555555555554 106 23 -3.739583333333333 106 24 -4.12337962962963 106 25 -3.7974537037037033 106 26 -2.2814814814814817 106 27 -2.9400462962962965 106 28 -2.0449074074074067 106 29 -1.8835648148148145 106 30 -1.2831018518518515 106 31 -1.4548611111111107 106 32 -1.2057870370370374 106 33 -1.4872685185185182 106 34 -1.555787037037038 106 35 -1.2939814814814818 106 36 -1.9483796296296294 106 37 -1.7824074074074074 106 38 -1.6664351851851844 106 39 -2.046296296296296 106 40 -1.9657407407407401 108 1 12.909953703703703 108 2 -4.609953703703702 108 3 -5.59212962962963 108 4 -5.177314814814814 108 5 -4.162500000000001 108 6 -5.806712962962964 108 7 -7.089814814814815 108 8 -5.827546296296298 108 9 -6.531712962962963 108 10 -4.882638888888888 108 11 -7.528472222222222 108 12 -8.131944444444446 108 13 -8.797685185185186 108 14 -8.44351851851852 108 15 -7.814583333333333 108 16 -6.655555555555557 108 17 -6.117361111111112 108 18 -5.773842592592592 108 19 -5.408564814814815 108 20 -5.476851851851853 108 21 -4.0643518518518515 108 22 -3.7430555555555554 108 23 -3.739583333333333 108 24 -4.12337962962963 108 25 -3.7974537037037033 108 26 -2.2814814814814817 108 27 -2.9400462962962965 108 28 -2.0449074074074067 108 29 -1.8835648148148145 108 30 -1.2831018518518515 108 31 -1.4548611111111107 108 32 -1.2057870370370374 108 33 -1.4872685185185182 108 34 -1.555787037037038 108 35 -1.2939814814814818 108 36 -1.9483796296296294 108 37 -1.7824074074074074 108 38 -1.6664351851851844 108 39 -2.046296296296296 108 40 -1.9657407407407401 112 1 4.743287037037035 112 2 0.3900462962962976 112 3 2.741203703703704 112 4 4.489351851851852 112 5 0.8374999999999986 112 6 2.5266203703703702 112 7 5.910185185185185 112 8 -1.1608796296296315 112 9 4.134953703703703 112 10 -1.215972222222221 112 11 5.471527777777778 112 12 9.201388888888886 112 13 8.535648148148146 112 14 8.889814814814812 112 15 7.185416666666667 112 16 4.344444444444443 112 17 7.882638888888888 112 18 2.892824074074074 112 19 9.258101851851853 112 20 1.856481481481481 112 21 -3.7310185185185185 112 22 0.25694444444444464 112 23 -2.739583333333333 112 24 4.209953703703704 112 25 -1.1307870370370363 112 26 -1.6148148148148147 112 27 5.393287037037037 112 28 -1.7115740740740737 112 29 5.7831018518518515 112 30 0.05023148148148149 112 31 2.5451388888888893 112 32 1.4608796296296296 112 33 0.8460648148148149 112 34 6.110879629629628 112 35 2.372685185185185 112 36 8.384953703703705 112 37 8.550925925925927 112 38 8.66689814814815 112 39 8.287037037037038 112 40 8.367592592592594 114 1 -13.590046296296297 114 2 -4.609953703703702 114 3 -5.59212962962963 114 4 -5.177314814814814 114 5 -4.162500000000001 114 6 -5.806712962962964 114 7 -7.089814814814815 114 8 -5.827546296296298 114 9 -6.531712962962963 114 10 -4.882638888888888 114 11 -7.528472222222222 114 12 -8.131944444444446 114 13 -8.797685185185186 114 14 -8.44351851851852 114 15 -7.814583333333333 114 16 -6.655555555555557 114 17 -6.117361111111112 114 18 -5.773842592592592 114 19 -5.408564814814815 114 20 -5.476851851851853 114 21 -4.0643518518518515 114 22 -3.7430555555555554 114 23 -3.739583333333333 114 24 -4.12337962962963 114 25 -3.7974537037037033 114 26 -2.2814814814814817 114 27 -2.9400462962962965 114 28 -2.0449074074074067 114 29 -1.8835648148148145 114 30 -1.2831018518518515 114 31 -1.4548611111111107 114 32 -1.2057870370370374 114 33 -1.4872685185185182 114 34 -1.555787037037038 114 35 -1.2939814814814818 114 36 -1.9483796296296294 114 37 -1.7824074074074074 114 38 -1.6664351851851844 114 39 -2.046296296296296 114 40 -1.9657407407407401 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 686.0 5 7.0 6 4.0 7 6.0 8 9.0 9 18.0 10 44.0 11 37.0 12 27.0 13 35.0 14 28.0 15 37.0 16 33.0 17 16.0 18 25.0 19 24.0 20 34.0 21 22.0 22 23.0 23 18.0 24 19.0 25 11.0 26 13.0 27 8.0 28 4.0 29 5.0 30 1.0 31 1.0 32 1.0 33 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 98.83040935672514 1.0025062656641603 0.0835421888053467 0.0835421888053467 8 97.24310776942356 1.0025062656641603 0.3341687552213868 1.4202172096908938 9 96.73366834170855 1.9262981574539362 0.5862646566164154 0.7537688442211055 10 43.19131161236424 34.83709273182957 10.526315789473683 11.445279866332497 11 32.748538011695906 26.4828738512949 23.475355054302423 17.293233082706767 12 37.510442773600666 16.95906432748538 22.807017543859647 22.7234753550543 13 25.98162071846282 18.546365914786968 25.146198830409354 30.32581453634085 14 20.30075187969925 24.143692564745194 31.57894736842105 23.976608187134502 15 25.48036758563074 21.4703425229741 29.57393483709273 23.475355054302423 16 29.908103592314117 20.96908939014202 26.065162907268167 23.05764411027569 17 24.31077694235589 26.81704260651629 25.98162071846282 22.890559732664993 18 28.404344193817877 24.895572263993316 23.642439431913115 23.05764411027569 19 19.563392107472712 21.998320738874895 18.387909319899247 40.050377833753146 20 25.730994152046783 25.229741019214703 22.305764411027567 26.733500417710943 21 23.05764411027569 23.893065998329156 27.151211361737676 25.898078529657475 22 20.2172096908939 20.885547201336674 29.490392648287383 29.406850459482037 23 29.32330827067669 20.96908939014202 26.399331662489555 23.308270676691727 24 24.06015037593985 18.71345029239766 24.394319131161236 32.83208020050125 25 22.761506276150627 23.682008368200837 23.263598326359833 30.2928870292887 26 20.42016806722689 22.941176470588236 28.067226890756302 28.57142857142857 27 19.46532999164578 18.880534670008352 28.57142857142857 33.08270676691729 28 19.883040935672515 22.305764411027567 26.649958228905597 31.161236424394318 29 19.430485762144052 18.592964824120603 32.32830820770519 29.64824120603015 30 17.099748533109807 16.177703269069575 33.94803017602682 32.774518021793796 31 21.998320738874895 18.639798488664987 34.34089000839631 25.02099076406381 32 26.337792642140467 14.297658862876252 32.85953177257525 26.505016722408026 33 19.46532999164578 16.374269005847953 38.93065998329156 25.229741019214703 34 17.293233082706767 18.629908103592314 42.10526315789473 21.97159565580618 35 16.207184628237258 19.46532999164578 35.08771929824561 29.239766081871345 36 13.199665831244777 23.893065998329156 36.6750208855472 26.232247284878863 37 17.627401837928154 18.796992481203006 36.340852130325814 27.234753550543022 38 15.204678362573098 22.7234753550543 32.999164578111944 29.07268170426065 39 20.80200501253133 19.21470342522974 34.67000835421888 25.31328320802005 40 14.452798663324979 20.71846282372598 36.25730994152047 28.57142857142857 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 3.0 20 6.0 21 9.5 22 13.0 23 13.0 24 12.0 25 11.0 26 12.0 27 13.0 28 13.0 29 16.0 30 19.0 31 19.5 32 20.0 33 20.0 34 26.0 35 32.0 36 44.5 37 57.0 38 57.0 39 51.0 40 45.0 41 57.0 42 69.0 43 69.0 44 76.0 45 83.0 46 79.0 47 75.0 48 75.0 49 79.5 50 84.0 51 76.5 52 69.0 53 69.0 54 65.0 55 61.0 56 67.0 57 73.0 58 73.0 59 78.5 60 84.0 61 89.5 62 95.0 63 95.0 64 90.0 65 85.0 66 80.5 67 76.0 68 76.0 69 64.0 70 52.0 71 46.0 72 40.0 73 40.0 74 30.5 75 21.0 76 14.5 77 8.0 78 8.0 79 6.0 80 4.0 81 2.5 82 1.0 83 1.0 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.2506265664160401 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5012531328320802 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.1670843776106934 26 0.5847953216374269 27 0.0 28 0.0 29 0.2506265664160401 30 0.3341687552213868 31 0.5012531328320802 32 0.0835421888053467 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1197.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.57727652464494 #Duplication Level Percentage of deduplicated Percentage of total 1 97.94520547945206 95.57226399331662 2 1.7123287671232876 3.341687552213868 3 0.2568493150684931 0.7518796992481203 4 0.08561643835616438 0.3341687552213868 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CACAAGGGGAGGCAGGAAAAATACTGGTGTATTACAAGGA 4 0.3341687552213868 No Hit CACAAGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 3 0.2506265664160401 No Hit CACAAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.2506265664160401 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 3 0.2506265664160401 No Hit CACAAGGGGAATCAACGCAGAGTCGACTTTTTTTTTTTTT 2 0.1670843776106934 No Hit CACAAGGGGAGCAGAGGTATAACACCTCACATAGAGAAAT 2 0.1670843776106934 No Hit CACAAGGGGAGAGCCCGGGCTTCTACAGGAACGTGGTGAA 2 0.1670843776106934 No Hit CACAAGGGGGCCGAATAGCCTCTCCACCCCAGCGGCCCGA 2 0.1670843776106934 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTCAGAGC 2 0.1670843776106934 No Hit CACAAGGGGTACAACGCAGGGTCGATTTTTTTTTTTTTTT 2 0.1670843776106934 No Hit CACAAGGGGAATCAGTCGCCTTTTTTTTTTTTTTTTTTTT 2 0.1670843776106934 No Hit CACAAGGGGATCCAGTGACAGATACCATTAGATTCAAGAG 2 0.1670843776106934 No Hit CACAAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGC 2 0.1670843776106934 No Hit CACAAGGGGAAGTATTGTTATGTTATACCCGGCAGTGAGT 2 0.1670843776106934 No Hit CACAAGGGGAAGCATGTATAACGCAGAGTCGACTTTTTTT 2 0.1670843776106934 No Hit CACAAGGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG 2 0.1670843776106934 No Hit CACAAGGGGGTAGCATCATCTTCTATAAAGAACCCAGGAG 2 0.1670843776106934 No Hit CACAAGGGGAGTCACAACCCTTTCACTGTTTGTCATTTCT 2 0.1670843776106934 No Hit CACAAGGGGATAGTTCATGTTAATAGTGGGGCTTTCCGGT 2 0.1670843776106934 No Hit CACAAGGGGAAGGCTACATTTGCTTGCTTTCTGCTGGCCC 2 0.1670843776106934 No Hit CACAAGGGGAAGGCTACATTTGCTTGCTTTCTGCTGGCCG 2 0.1670843776106934 No Hit CACAAGGGGTTTTGTAAAGATGTATAAGAGTAAGAAAACT 2 0.1670843776106934 No Hit CACAAGGGGGAAAAAAAACAAAAAAAAAAAAAAAAAAAAA 2 0.1670843776106934 No Hit CACAAGGGGGTTTTTGTTCTTGTGTGTGTGTGTTTGTGTT 2 0.1670843776106934 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGGGGA 45 6.657501E-10 34.000004 4 ACAAGGG 110 0.0 34.000004 2 CAAGGGG 110 0.0 34.000004 3 AGGGGGG 20 0.0017877228 34.0 5 CACAAGG 115 0.0 34.0 1 AAGGGGG 40 1.4437319E-8 34.0 4 AAGGGGC 20 0.0017877228 34.0 4 >>END_MODULE