FastQCFastQC Report
Fri 17 Jun 2016
SRR1526138_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526138_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37886
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG3250.857836667898432No Hit
AGGACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT2510.6625138573615584No Hit
AGGACTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC2350.6202818983265586No Hit
AGGACTGGGCTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATC1020.2692287388481233No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC980.2586707490893734No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG790.2085202977353112No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC760.20060180541624875No Hit
AGGACTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC750.19796230797656128No Hit
AGGACTGGGCTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACT720.1900438156574988No Hit
AGGACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT600.158369846381249No Hit
AGGACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA580.15309085150187404No Hit
AGGACTGGGGCTAGGACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAA520.13725386686374916No Hit
AGGACTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC440.11613788734624927No Hit
AGGACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG420.1108588924668743No Hit
AGGACTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT400.10557989758749933No Hit
AGGACTGGGATTTGGGCTGTTCCCACACTTTAGCCGCTGTTTATAGTGTTGCTATG390.10294040014781186No Hit
AGGACTGGGCTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATC380.10030090270812438No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAATCC251.8879207E-550.6936641
TTTCCAT150.00796611350.6253438
TTGTCCA251.9030947E-550.62533637
TGTCCAA251.9030947E-550.62533638
CCACGAT150.00818084950.2864846
CATCTGG150.00818084950.2864842
CTGGTTC150.00818084950.2864845
ATTCAGG150.00822430850.2192515
GAACGTC150.00835571450.01864250
GCAGTGA150.00835571450.0186429
AAGGTCA150.008488676549.8196317
ATCAACG355.2561518E-849.8196320
AACGCAG204.2342182E-449.75364323
ACGCAGA150.00853334649.75364324
TGGGTAC750.049.6221966
GGATGCC252.1431133E-549.6221928
GGGCAAC150.00862320649.6221927
AGGACTA355.4238626E-849.6221921
CTGGGTA1000.049.6221925
TCAACGC355.4238626E-849.62219221
Produced by FastQC (version 0.11.5)