FastQCFastQC Report
Fri 17 Jun 2016
SRR1526137_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526137_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4491
Sequences flagged as poor quality0
Sequence length40
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG270.6012024048096193No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC170.37853484747272326No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCG150.33400133600534404No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT140.31173458027165446No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC130.2894678245379648No Hit
AGGACTGGGGTTGTGTGGCTTTGGCCCTAAGCCATTGTCT120.26720106880427524No Hit
AGGACTGGGTGCATCCTATGTGGACTTTCTCTGTCATATG90.2004008016032064No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC80.1781340458695168No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGCGCG60.13360053440213762No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT60.13360053440213762No Hit
AGGACTGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT60.13360053440213762No Hit
AGGACTGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT50.111333778668448No Hit
AGGACTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT50.111333778668448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGAG301.1799473E-534.0000045
TGGGGGG301.1799473E-534.0000046
TGGGGCC301.1799473E-534.0000046
CTGGGTG251.7228849E-434.05
CTGGGGT358.033021E-734.05
CTGGGAT502.419256E-1034.05
GGCTAGG502.419256E-1034.09
GGGGCTA502.419256E-1034.07
GGGGCCG251.7228849E-434.07
TGGGATG251.7228849E-434.06
GGGCTAG502.419256E-1034.08
TAGGACT405.4315024E-833.99999612
GCTAGGA405.4315024E-833.99999610
GGCCGAA200.002502351533.9999969
CTAGGAC405.4315024E-833.99999611
TGGGGTC200.002502351533.9999966
TGGGGGA200.002502351533.9999966
GGGGGGG200.002502351533.9999967
CGAATAG200.002502351533.99999612
ACTGGGT405.4315024E-833.9999964
Produced by FastQC (version 0.11.5)