FastQCFastQC Report
Fri 17 Jun 2016
SRR1526135_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526135_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC331.7223382045929019No Hit
AGGACTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT251.3048016701461378No Hit
AGGACTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG160.8350730688935281No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT80.41753653444676403No Hit
AGGACTGGGCTCACAGATCCTCTTCTGAGATGAGTTTTTG80.41753653444676403No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG70.36534446764091855No Hit
AGGACTGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT70.36534446764091855No Hit
AGGACTGGGCTTCTGGCTTTCATAGTCTCTGGTGAAAAAT60.31315240083507306No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC50.2609603340292276No Hit
AGGACTGGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAG40.20876826722338201No Hit
AGGACTGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT40.20876826722338201No Hit
AGGACTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT40.20876826722338201No Hit
AGGACTGGGCTCACAGATCCTCTTCTGAGATGAGGATCGG40.20876826722338201No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA40.20876826722338201No Hit
AGGACTGGGGCTAGGACTGGGTCACAGATCCTCTTCTGAG30.15657620041753653No Hit
AGGACTGGGCAAGCTGAAAGGTTACACATCCTGGGCCATT30.15657620041753653No Hit
AGGACTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG30.15657620041753653No Hit
AGGACTGGGGGGAAGAGGGTGGAGAACAAGCTCCAATCCC30.15657620041753653No Hit
AGGACTGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC30.15657620041753653No Hit
AGGACTGGGGCTGGTGAGATGGCTCAATAGATAAGAGCAC30.15657620041753653No Hit
AGGACTGGAATTGCAACTTGTCCTTTTTTCTTGGCATTGC30.15657620041753653No Hit
AGGACTGGGGAGATGAGATGTCATAAGGAGATAAATTATT30.15657620041753653No Hit
AGGACTGGGGTGTCTGTTCCTCACCCAATTTTTAGTTGCT30.15657620041753653No Hit
AGGACTGGGCTCACAGATCCTCTTCTGAAGGAGATTGGGC30.15657620041753653No Hit
AGGACTGGGATCTCAATTTTAGGATCTTAATTACCACACC20.10438413361169101No Hit
AGGACTGGGATCTTCCTTTGTGCTTCCCCTGAGCACACCA20.10438413361169101No Hit
AGGACTGGGATGCCACAACTAGATACATCAACATGATTTA20.10438413361169101No Hit
AGGACTGGGAGAAAGAAGGGTTCCAAAACAGCCTCTGAGG20.10438413361169101No Hit
AGGACTGGGGGAGGTAATATTAAATGATCTCCAGCTATAC20.10438413361169101No Hit
AGGACTGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG20.10438413361169101No Hit
AGGACTGGGGGGCTGGAGATATGGCTCAGTGGTTAAGAGC20.10438413361169101No Hit
AGGACTGGGAGCATATGCTTGTCTCAAAGATTAAGCCATG20.10438413361169101No Hit
AGGACTGGGGGGGTTGGGGATTTAGATCAGTGGTAGAGAG20.10438413361169101No Hit
AGGACTGGGTGAGTCGACTTTTTTTTTTTTTTTTTTTTTT20.10438413361169101No Hit
AGGACTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10438413361169101No Hit
AGGACTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAG20.10438413361169101No Hit
AGGACTGGGAAGATTGAGGTTGTGAAAATATTGATTACTT20.10438413361169101No Hit
AGGACTGGGTTGTTATATAGGGCAGGATAGAATGCATGTC20.10438413361169101No Hit
AGGACTGGGACTGCCCTAGTTAGGACAGACATGCTTGTCT20.10438413361169101No Hit
AGGACTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10438413361169101No Hit
AGGACTGGGGGAAGGGTGGGTGAAAATAAAAGTTGAATAT20.10438413361169101No Hit
AGGACTGGCATTCGAACGTCTGCCCTATCAACTTTCGATG20.10438413361169101No Hit
AGGACTGGGGTCAGTGTGGGTTTTGTGGAGGCAATAAGAC20.10438413361169101No Hit
AGGACTGGGGGCTGGAGAGATGGCTCACAGGTTAAGAGCA20.10438413361169101No Hit
AGGACTGGGCATTCGATCGGTTGAGAAAATTGAGAAAATC20.10438413361169101No Hit
AGGACTGGGATTATTAGAATTTTTTTTTCTTAGGGAAACA20.10438413361169101No Hit
AGGACTGGGCATATAATAAATAAATAAATCTTAAAAAAAA20.10438413361169101No Hit
AGGACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGG20.10438413361169101No Hit
AGGACTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.10438413361169101No Hit
AGGACTGGGGCTAGGAATGGGGCTAGGACTGGGGTGGAAT20.10438413361169101No Hit
AGGACTGGGGTCTGAGGGGCATTCGTATTGCGCCGCTAGA20.10438413361169101No Hit
AGGACTGGGGATTTCCTTGAATGTGGCTTAGGAAATGTGG20.10438413361169101No Hit
AGGACTGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGTT20.10438413361169101No Hit
AGGACTGGGACCAGGGCATGTGGGGAATAAGACACTGTTA20.10438413361169101No Hit
AGGACTGGGGGAGAGGGAGCCTGAGAAACGGCTACCACAT20.10438413361169101No Hit
AGGACTGGGCCCGTGGTCCACATGGAGATCCCAGGAGAGA20.10438413361169101No Hit
AGGACTGGGGTACCTGGTTGATCCTGCCAGTAGCATATGC20.10438413361169101No Hit
AGGACTGGATCAATGGGAGCAGTGTTTGCTATCATAGCAG20.10438413361169101No Hit
AGGACTGGGATATCTAGTGATTTCTAAGGATTTGTTTACT20.10438413361169101No Hit
AGGACTGGGTCACAAATCCTCTTCTGAGATGAGTTTTTGT20.10438413361169101No Hit
AGGACTGGGAAGGTTCGGATGAATGCCAGGACCAAGTTTA20.10438413361169101No Hit
AGGACTGGGGGGCTGGAGGAATGGCTCAGTGGTTAAGAGC20.10438413361169101No Hit
AGGACTGGGGGAAGGGTTATGCAGACATTGTCCAGTTGCT20.10438413361169101No Hit
AGGACTGGGGGGCAGACACTCCAAGTTTGCTGAGCAAAGC20.10438413361169101No Hit
AGGACTGGGACTTTTATGCAGTCTTTTGGTTCAAGGAAAC20.10438413361169101No Hit
AGGACTGGGAGTGTGCATGCAGATACACATGGCATATTGC20.10438413361169101No Hit
AGGACTGGGTGCTAGGACTGGGTCACAGATCCTCTTCTGA20.10438413361169101No Hit
AGGACTGGGTCTTTGCCCCATCTACTGCGAGGATGAAGAC20.10438413361169101No Hit
AGGACTGGGCTCAAAGATCCTCTTCTGAGATGAGTTTTTG20.10438413361169101No Hit
AGGACTGGGAGCCACGTTGCTCAGCTACCAAAGACTCCTT20.10438413361169101No Hit
AGGACTGGGGCTCTCTTTGCCCCATCTACTGCGAGGATGA20.10438413361169101No Hit
AGGACTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC20.10438413361169101No Hit
AGGACTGGGAACTGTGTAATTTCTGGTCGGTAATTCCAGG20.10438413361169101No Hit
AGGACTGGGGGGGACTGGAGAGATGGCTCAGCGGTTAAGA20.10438413361169101No Hit
AGGACTGGGATGGTGAAATGTAAAAGAGAAGGAGATTTCC20.10438413361169101No Hit
AGGACTGGGACCTCGGCTCTGCGCTCTTGGCCGCTTCCAA20.10438413361169101No Hit
AGGACTGAGGCTGACTAAGTTGTCCCGAGAACTGACTTTC20.10438413361169101No Hit
AGGACTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAG20.10438413361169101No Hit
AGGACTGGGGCTAGGACTGGGGGGCTGGTGAGATGGCTCA20.10438413361169101No Hit
AGGACTGGGAGGGTAATCTCAGGAGGGTTGTCTACCTTTA20.10438413361169101No Hit
AGGACTGGGAGCTGTGAAAGAAAGAATTGCCAAGTTCTTT20.10438413361169101No Hit
AGGACTGGCGCTAGGACTGGGGAGGAGAAGCCCGGTTTAC20.10438413361169101No Hit
AGGACTGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGA20.10438413361169101No Hit
AGGACTGGGAAGACTAAGGGAGATGCTTTGGTTTTCTTAG20.10438413361169101No Hit
AGGACTGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT20.10438413361169101No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGGA251.1919941E-434.7297335
CTGGGAA251.1919941E-434.7297335
TGGGGGG251.1919941E-434.7297336
CTGGGGG402.4470864E-834.729735
ACTGGGG800.034.729734
ACTGGGA800.034.729734
GACTGGG1900.033.815793
AGGACTG2000.032.1251
GGACTGG2000.032.1252
CTGGGAT303.4863353E-428.941445
Produced by FastQC (version 0.11.5)