FastQCFastQC Report
Fri 17 Jun 2016
SRR1526134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67393
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC2210.3279272327986586No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2000.2967667265146232No Hit
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2000.2967667265146232No Hit
CAAAGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGGGCACTGACTGTTCTTCT1840.27302538839345336No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC1200.17806003590877392No Hit
CAAAGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT950.140964195094446No Hit
CAAAGTGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTG900.13354502693158044No Hit
CAAAGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT840.12464202513614174No Hit
CAAAGTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC770.11425518970812994No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC740.10980368881041058No Hit
CAAAGTGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTAC700.1038683542801181No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGAA150.00817768550.33508340
GCCCGTC150.00817768550.33508339
ATGCTTG252.0038662E-550.2597336
TATGCTG252.0038662E-550.2597336
GAGCGTC150.00829963950.14712534
ATATGCT355.0709787E-850.1471234
CTCGTGC150.00834879750.07233445
TCGTGCG150.00834879750.07233446
GTCTCGT150.00837345850.03502343
ATAACAA150.00842294149.96056729
CGGAGCG252.0760286E-549.96056732
TGGTATC150.00842294149.96056716
GCATATG355.2225005E-849.96056432
ACAAAAC150.00847264249.8863323
GAAAGCT150.00847264249.8863323
TCGTGAC150.00847264249.8863322
TGGGGTA650.049.8492976
TGGGATC2100.049.8492976
TGGGACT1150.049.8492976
GGATAGC150.00849757349.8492938
Produced by FastQC (version 0.11.5)