##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526134_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	67393
Sequences flagged as poor quality	0
Sequence length	56
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.24008428175033	38.0	36.0	38.0	33.0	40.0
2	34.306307776772066	38.0	35.0	38.0	23.0	40.0
3	34.75050821301916	38.0	35.0	38.0	26.0	40.0
4	33.53044084697224	38.0	35.0	38.0	14.0	40.0
5	34.00068256347098	38.0	33.0	38.0	24.0	40.0
6	32.893445906844924	36.0	31.0	39.0	21.0	39.0
7	36.09204220022851	38.0	36.0	39.0	30.0	40.0
8	35.82811271200273	38.0	36.0	39.0	29.0	40.0
9	35.84608193729319	38.0	36.0	39.0	29.0	40.0
10	34.12220853797872	38.0	33.0	39.0	24.0	40.0
11	33.911281587108455	37.0	32.0	39.0	25.0	40.0
12	33.17648717225825	37.0	31.0	39.0	21.0	40.0
13	32.895389728903595	36.0	31.0	39.0	19.0	40.0
14	33.552846734824094	38.0	32.0	39.0	22.0	40.0
15	32.72577270636416	37.0	31.0	39.0	17.0	40.0
16	32.70244684166011	37.0	30.0	39.0	19.0	40.0
17	31.7093021530426	36.0	28.0	39.0	15.0	40.0
18	30.861958957161722	35.0	26.0	38.0	13.0	40.0
19	32.235662457525265	36.0	29.0	39.0	18.0	40.0
20	32.87598118498954	36.0	30.0	39.0	21.0	40.0
21	32.98983573961687	38.0	30.0	39.0	19.0	40.0
22	32.24581187957206	37.0	29.0	39.0	16.0	40.0
23	32.907008146246646	37.0	30.0	39.0	19.0	40.0
24	32.36761978246999	37.0	29.0	39.0	17.0	40.0
25	32.84703159081804	37.0	30.0	39.0	19.0	40.0
26	33.45853426913774	38.0	33.0	39.0	20.0	40.0
27	33.60765954921134	38.0	33.0	39.0	21.0	40.0
28	33.61350585372368	38.0	33.0	39.0	22.0	40.0
29	33.173400798302495	38.0	32.0	39.0	19.0	40.0
30	32.93938539610939	37.0	31.0	39.0	18.0	40.0
31	33.788909827430146	38.0	33.0	39.0	22.0	40.0
32	33.700280444556554	38.0	33.0	39.0	22.0	40.0
33	33.5144451204131	38.0	33.0	39.0	21.0	40.0
34	33.32021129790928	38.0	33.0	39.0	20.0	40.0
35	33.267564880625585	38.0	33.0	39.0	20.0	40.0
36	32.494992061490066	36.0	31.0	39.0	18.0	40.0
37	32.218034513970295	36.0	31.0	39.0	16.0	40.0
38	31.756042912468654	36.0	30.0	38.0	11.0	40.0
39	30.836451857017792	35.0	29.0	38.0	4.0	40.0
40	28.313088896472927	34.0	21.0	38.0	4.0	39.0
41	26.961494517234726	32.0	18.0	38.0	4.0	39.0
42	27.87960173905302	33.0	22.0	38.0	4.0	39.0
43	27.571513361921863	32.0	21.0	38.0	4.0	39.0
44	26.73148546584957	31.0	18.0	37.0	4.0	39.0
45	27.75003338625673	33.0	23.0	38.0	4.0	39.0
46	25.862344753906193	30.0	18.0	36.0	4.0	39.0
47	24.6732598341074	29.0	12.0	36.0	4.0	39.0
48	25.648865609187897	30.0	18.0	36.0	4.0	39.0
49	24.404062736485987	29.0	10.0	36.0	4.0	39.0
50	25.164201029780543	30.0	16.0	36.0	4.0	39.0
51	25.490481207247043	31.0	15.0	36.0	4.0	39.0
52	25.20653480331785	30.0	10.0	36.0	4.0	39.0
53	25.112326205985784	31.0	4.0	36.0	4.0	39.0
54	24.78560087842951	30.0	4.0	36.0	4.0	39.0
55	24.270948021307852	30.0	4.0	36.0	4.0	39.0
56	23.653213241731336	30.0	4.0	36.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	3.506580400309012
1	2	5.998519333669293
1	3	5.307265357496483
1	4	6.959343885234944
1	5	6.274994498002151
1	6	6.989259309107791
1	7	4.28803862958604
1	8	4.511120972241592
1	9	4.491675166251177
1	10	5.955738426872536
1	11	-2.702301604476439
1	12	-7.846266093375821
1	13	-19.10357855043518
1	14	-8.117795848044615
1	15	-0.6468466360231275
1	16	-0.8963484054185642
1	17	-5.447811786804458
1	18	-3.832852386603438
1	19	0.3025993238466995
1	20	-0.908606466428715
1	21	6.370949858854601
1	22	7.267856752178286
1	23	6.234530381252096
1	24	6.780724959206804
1	25	6.344561122625439
1	26	6.766692557265685
1	27	5.996770389915376
1	28	6.019898450954148
1	29	6.895706968037501
1	30	6.850571293063698
1	31	5.744382854193116
1	32	5.598797653314655
1	33	5.7046577261771425
1	34	5.676899096797829
1	35	6.071464787520739
1	36	5.025560820621042
1	37	5.666404372663205
1	38	6.095162792359968
1	39	6.918590762033215
1	40	-8.08588995598226
1	41	1.5764125099982813
1	42	0.4817192143198703
1	43	-8.859896711216098
1	44	-14.882053880178217
1	45	-0.5164029733453859
1	46	-4.325745339244264
1	47	-10.464184537409082
1	48	-3.9470823179945107
1	49	-14.292831279710668
1	50	-2.557435111212662
1	51	4.451428632107003
1	52	4.245377332617238
1	53	4.6349288000567945
1	54	4.703182592941747
1	55	5.10767533176886
1	56	8.153537038720643
2	1	3.5072573910850124
2	2	6.156258184477451
2	3	5.5703608978197465
2	4	7.097365379692086
2	5	6.399053057704272
2	6	7.2925511767560955
2	7	4.586845433343342
2	8	4.536000383259612
2	9	4.513846614165196
2	10	5.984510534852568
2	11	2.282212231522543
2	12	-1.506416723823481
2	13	0.535162246721459
2	14	4.400102095595905
2	15	5.042160726251566
2	16	4.529817288073865
2	17	6.82941593596782
2	18	5.762053257807157
2	19	5.835560312253232
2	20	5.694253819599886
2	21	6.348016796317573
2	22	7.198634445332214
2	23	6.512604342494374
2	24	7.522199106621546
2	25	5.839864499116938
2	26	5.933570783549854
2	27	5.648797131051033
2	28	5.350693068877472
2	29	5.8966378303545
2	30	6.479664971662327
2	31	4.332010847761705
2	32	5.3408641676584026
2	33	4.873651548636307
2	34	3.9075837037200607
2	35	4.694719420676364
2	36	4.649238573011662
2	37	3.9633494517865024
2	38	4.461414802176332
2	39	4.25488593001155
2	40	2.139886467813966
2	41	6.826983720965529
2	42	5.827323005468216
2	43	5.3910976189861515
2	44	-4.62632061454396
2	45	5.481481430479612
2	46	4.352176299073413
2	47	0.2938758840176767
2	48	3.2844485273977213
2	49	-1.5635429662639382
2	50	4.5931953364474865
2	51	6.471399859999025
2	52	7.362750608406355
2	53	7.21959495898038
2	54	7.34640845398939
2	55	7.842802690658598
2	56	7.739980298431231
3	1	3.413316609644234
3	2	5.830556108775376
3	3	5.570437210396058
3	4	6.834697492024198
3	5	6.039315572966785
3	6	6.805295377000295
3	7	3.973063382061291
3	8	4.130551665310897
3	9	4.144875307693894
3	10	5.6661344664765
3	11	4.166598678408988
3	12	4.862096707189949
3	13	5.522570671629886
3	14	4.874690007683817
3	15	-0.12633744224660504
3	16	5.844988228244802
3	17	7.57048736453925
3	18	8.222752281006183
3	19	6.003447980140901
3	20	5.598023660869728
3	21	6.578251839052619
3	22	7.949168633366401
3	23	7.017946222836255
3	24	7.265254662177103
3	25	6.814681348933785
3	26	5.915561015540085
3	27	5.496858791612688
3	28	5.3584769516613555
3	29	5.938228184444853
3	30	6.102604532101889
3	31	4.184269700020561
3	32	4.6144447537389865
3	33	5.3511393386240975
3	34	3.8102088563452092
3	35	4.254014292471233
3	36	5.139775813548905
3	37	5.507458120895169
3	38	6.2912377569992906
3	39	6.967034892160516
3	40	7.815100143027642
3	41	10.9087144901963
3	42	9.919661222806432
3	43	10.314785042673577
3	44	10.943352767629424
3	45	9.298483872482056
3	46	11.560128069525184
3	47	12.132703722845516
3	48	10.870986988936181
3	49	12.504375226654254
3	50	10.749407180159334
3	51	10.302825378924545
3	52	10.666169411825159
3	53	10.187085801471223
3	54	10.478200273281207
3	55	11.09936557222148
3	56	9.57583194678288
4	1	3.6781975620251828
4	2	5.920503198722464
4	3	5.446429273888121
4	4	6.97557050789721
4	5	6.047913456564672
4	6	6.864488498693415
4	7	4.192970789468696
4	8	4.2624961097553395
4	9	4.167692768011349
4	10	5.767986318328354
4	11	5.742326191636508
4	12	6.51851205110529
4	13	6.474620936180145
4	14	5.863777309271114
4	15	6.498001182092018
4	16	7.710728968985542
4	17	8.477564084115965
4	18	8.873876619630519
4	19	7.6767284034213255
4	20	7.027587152933222
4	21	7.362974061274841
4	22	8.118150114847882
4	23	6.883686963576995
4	24	7.735162069584508
4	25	6.987300396552836
4	26	5.588841438820509
4	27	6.100364082617979
4	28	5.580037798222204
4	29	5.779828713545385
4	30	5.946189188186548
4	31	4.837708853459713
4	32	5.175622002416233
4	33	5.2668139417986986
4	34	5.044335840472197
4	35	5.446143922100859
4	36	4.914908088681177
4	37	5.17346341190046
4	38	5.588195428956961
4	39	5.52126769639332
4	40	1.426211254138753
4	41	9.82769597167778
4	42	8.999687677832888
4	43	9.730128958017488
4	44	10.029662291438942
4	45	8.660256359254536
4	46	11.119982566879678
4	47	11.648576738718528
4	48	11.699690692639884
4	49	12.202126549405577
4	50	12.02553151878367
4	51	10.662283050882216
4	52	10.499502745158495
4	53	9.82002230940773
4	54	10.585012442593378
4	55	10.860608958464866
4	56	9.02986633831727
5	1	3.2545215495641244
5	2	5.371610848428247
5	3	4.589212243773893
5	4	6.547481203639684
5	5	5.758107295263187
5	6	6.066894175398158
5	7	3.9000493633697033
5	8	3.687210543815567
5	9	4.031140327720593
5	10	4.888097083298931
5	11	5.086909092294171
5	12	5.648228215868187
5	13	6.050858388585823
5	14	5.842230061088351
5	15	6.437080448274088
5	16	7.4512966394597555
5	17	8.343434627556608
5	18	9.193797007307918
5	19	7.673007392690966
5	20	6.885323393847031
5	21	7.2038359512021515
5	22	8.087603212805657
5	23	6.720049026574575
5	24	7.7132686808686906
5	25	6.6656493062101525
5	26	5.540727904725664
5	27	5.3774495793296495
5	28	5.993156524425043
5	29	6.011742870693183
5	30	6.129557135573183
5	31	4.144216295481176
5	32	4.801444086182244
5	33	4.639607295900465
5	34	4.442051312954025
5	35	4.912308684994599
5	36	6.049989431706447
5	37	5.915502179640168
5	38	6.077118931899154
5	39	7.521440766659847
5	40	9.534726311251944
5	41	10.652289256551438
5	42	10.665488993166917
5	43	10.072461945210293
5	44	11.080285344398444
5	45	10.07034635579313
5	46	11.637981874598616
5	47	12.10600145315259
5	48	11.26000568052497
5	49	12.130042783396576
5	50	11.181900504591905
5	51	10.473636460366464
5	52	10.315702122105534
5	53	10.198267376905132
5	54	8.968103477553573
5	55	9.426635265145375
5	56	9.536529543578606
6	1	3.5478945317221573
6	2	5.861075589294856
6	3	5.4231969506558
6	4	6.890216972543676
6	5	5.966263624914838
6	6	6.743444727649649
6	7	4.299199745697656
6	8	4.236233483492718
6	9	4.25153115184974
6	10	6.316134466476498
6	11	5.6265012758115915
6	12	6.54797333056657
6	13	6.626304437863652
6	14	6.187514683008487
6	15	6.680156064246901
6	16	7.488170046426617
6	17	8.75668866324055
6	18	8.906031501785403
6	19	8.247603824296743
6	20	7.040718466064533
6	21	7.545297293598075
6	22	8.595759542457312
6	23	7.329309859199892
6	24	7.595936480358919
6	25	7.08578524503768
6	26	6.618807768786837
6	27	6.379488661742563
6	28	6.185425003609414
6	29	6.2286502623669335
6	30	6.84400063599799
6	31	5.041412557163415
6	32	5.239931766725995
6	33	6.068834143818901
6	34	5.579689375825733
6	35	5.711968837925781
6	36	6.561204384977476
6	37	6.457133445570495
6	38	7.1188351595966886
6	39	6.993658268783893
6	40	3.9332819612094596
6	41	10.6962144901963
6	42	10.24985602800124
6	43	10.5368629647515
6	44	10.567541079317731
6	45	10.153354002352184
6	46	12.194056640953754
6	47	12.295041385183179
6	48	11.618714261663456
6	49	13.096907694186719
6	50	11.639504582756732
6	51	12.020195508794671
6	52	11.911623957279701
6	53	12.193254632640052
6	54	11.857908065489003
6	55	11.541248689104592
6	56	11.216903375354306
7	1	3.4796160017414977
7	2	5.864750637650751
7	3	5.498438730152898
7	4	7.057721808133621
7	5	6.131640800930313
7	6	6.7496342827753715
7	7	4.450456999082569
7	8	4.541455920630042
7	9	4.534123028058637
7	10	6.086347232433944
7	11	5.978338799989537
7	12	6.277180293816087
7	13	6.647000762815296
7	14	6.520396694613908
7	15	6.963328211248836
7	16	7.1614775899469265
7	17	8.765586148733778
7	18	9.27005471261712
7	19	7.979131870718408
7	20	6.6327880985597005
7	21	7.358836945435598
7	22	8.099434590813207
7	23	7.329116435602213
7	24	7.588392960049443
7	25	7.052903233432264
7	26	6.705074693351634
7	27	5.80327976425707
7	28	6.014631966858925
7	29	6.188418154049714
7	30	6.453478392284257
7	31	4.7222636209932
7	32	5.15946755009157
7	33	5.1332442018459865
7	34	4.531333476406001
7	35	4.4814079094925106
7	36	6.386736299871092
7	37	6.222123774390614
7	38	6.869125294993207
7	39	7.513387475747141
7	40	7.910844823878705
7	41	10.851533639132473
7	42	10.695117149858106
7	43	10.898371668813397
7	44	11.134272220516959
7	45	10.31254162324193
7	46	11.43873749201758
7	47	13.225408890018766
7	48	11.554497627234056
7	49	12.734238448538754
7	50	12.272773441557508
7	51	11.283638448833358
7	52	11.804467284165582
7	53	11.241417108462105
7	54	11.232569574193061
7	55	11.966006909607493
7	56	10.710520396630905
8	1	3.3686737525013726
8	2	5.6417805985712945
8	3	5.097222924681773
8	4	6.791330145085418
8	5	5.914315572966785
8	6	6.217285172918661
8	7	4.147808280020477
8	8	4.011929216331303
8	9	4.319620205653074
8	10	5.099807935864256
8	11	5.871955821266134
8	12	6.179698748006274
8	13	6.6131318961196754
8	14	5.659128783194021
8	15	5.904274802651351
8	16	7.051620881306029
8	17	7.900844507396389
8	18	8.468925750393932
8	19	6.727937776059267
8	20	5.239605293522793
8	21	5.890751839052619
8	22	6.978505368060279
8	23	5.423558467734214
8	24	6.363468947891388
8	25	5.5366201244439885
8	26	4.941071219621719
8	27	4.13588940385759
8	28	5.070211645538908
8	29	5.138483286485673
8	30	5.213573919856987
8	31	3.1740656183879032
8	32	3.0595978149634746
8	33	2.6534352569914468
8	34	3.305106815528884
8	35	3.3930449047161275
8	36	4.10406152783462
8	37	4.081437712731908
8	38	4.457054083529901
8	39	5.3803001982829635
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115	22	6.058310424411179
115	23	5.527274581045209
115	24	6.421038244266658
115	25	5.821877510980695
115	26	4.7519149601029795
115	27	5.377589068798194
115	28	4.973349019891639
115	29	5.6366556897753455
115	30	6.415371056622146
115	31	4.593651363133564
115	32	5.227584412587596
115	33	4.064492217088915
115	34	4.802213120737537
115	35	5.208305337247353
115	36	5.729861101395382
115	37	5.47680780106575
115	38	6.273380614142145
115	39	6.443047685337486
115	40	8.77032402362466
115	41	7.790244340942568
115	42	8.13208126545036
115	43	6.607962014955024
115	44	-2.700437211048616
115	45	5.765701356490581
115	46	2.261220820058231
115	47	-7.43019606393487
115	48	1.2385243023690187
115	49	-2.2490895921090726
115	50	3.6821097387947255
115	51	4.548960773380831
115	52	3.9721395610788903
115	53	4.004516505095957
115	54	5.1017343884198
115	55	4.1017642929037805
115	56	6.413918300727442
116	1	3.071771859213939
116	2	5.109816005505152
116	3	4.301181616592274
116	4	6.118475461043126
116	5	5.6354859688359795
116	6	5.9299726575510725
116	7	4.065683863989001
116	8	4.095160099045835
116	9	3.947478577917643
116	10	4.964327237560838
116	11	3.739425700784203
116	12	3.4220149515961467
116	13	4.6123942516643055
116	14	4.084995515429078
116	15	-2.489504395775004
116	16	4.771193047521912
116	17	6.853512321509985
116	18	6.413479475498438
116	19	5.78571992506345
116	20	5.322098531781947
116	21	6.69195665832973
116	22	7.314379476739898
116	23	6.766440198739868
116	24	7.571730565791558
116	25	6.801557424665287
116	26	5.640604044799979
116	27	5.54860147663851
116	28	5.171514817410067
116	29	4.8083873927064715
116	30	6.349915203358339
116	31	5.101653521018836
116	32	5.08012891897134
116	33	4.83575637821102
116	34	5.067093537928688
116	35	5.120731159941116
116	36	6.244336915097957
116	37	6.968086348089667
116	38	7.3697661563108205
116	39	8.454556406790463
116	40	10.489798938208367
116	41	9.078142201039672
116	42	8.24087464793552
116	43	3.124595713930031
116	44	0.6250438175433608
116	45	7.563759259745392
116	46	2.103695195170623
116	47	-6.633217103316277
116	48	2.095384579297626
116	49	-2.121055065944713
116	50	3.354291001157609
116	51	6.4378296818505305
116	52	6.804723628692628
116	53	5.926113340197556
116	54	5.702705436792396
116	55	5.874107396662097
116	56	8.563891327161535
117	1	2.440102323929949
117	2	4.419841823061091
117	3	3.650794353253197
117	4	5.441840349167052
117	5	5.075029858681077
117	6	5.171366805571726
117	7	3.160563382061291
117	8	3.157337379596612
117	9	3.1359467362653177
117	10	3.9161344664764997
117	11	-1.6101870358767236
117	12	-8.539689007095763
117	13	-9.200643614084402
117	14	-3.9378099923161862
117	15	-8.233480299389463
117	16	-7.012154628898056
117	17	0.6508445073963927
117	18	-2.4290334332795354
117	19	0.6463051229980437
117	20	-0.3930477677016988
117	21	4.390751839052619
117	22	4.386668633366401
117	23	4.0358033656934005
117	24	5.006326090748534
117	25	4.439681348933785
117	26	5.683418158397224
117	27	5.023644505898403
117	28	5.001334094518501
117	29	4.7864424701591375
117	30	5.84367596067332
117	31	4.237841128591985
117	32	4.337659039453271
117	33	5.092210767195528
117	34	4.98878028491664
117	35	5.298657149614087
117	36	5.55049009926319
117	37	6.4717438351808845
117	38	6.219809185570714
117	39	7.377749177874801
117	40	9.547243000170496
117	41	6.685500204482015
117	42	5.330375508520721
117	43	-6.649500671612138
117	44	-4.637004375227722
117	45	4.619912443910625
117	46	-1.877371930474819
117	47	-4.1708677057259145
117	48	-0.8075844396352458
117	49	-3.3884819162028883
117	50	0.11547860873076132
117	51	5.4188968074959725
117	52	4.719740840396586
117	53	5.258514372899793
117	54	5.04070027328121
117	55	5.045794143650049
117	56	8.227617661068592
118	1	1.9330995228094991
118	2	4.31865134687061
118	3	3.1098279666985746
118	4	5.408577043844922
118	5	4.7238393824905955
118	6	4.952179130501694
118	7	2.731641813433839
118	8	3.1121693123697227
118	9	2.916058781083251
118	10	3.994565839025519
118	11	-3.775103002263279
118	12	-12.557196009896884
118	13	-9.065139412403731
118	14	-8.065961252820387
118	15	-8.29720578958554
118	16	-7.863345105088531
118	17	0.20196495557566152
118	18	-8.043879371654885
118	19	-1.9055156052932745
118	20	-1.6717592522955371
118	21	3.9985949763075226
118	22	5.386668633366401
118	23	3.6282403404833126
118	24	5.332656622961419
118	25	4.7569082396900875
118	26	5.227185665400029
118	27	4.275745346234537
118	28	4.748883114126347
118	29	5.376778604612923
118	30	4.502289406051471
118	31	3.4727851061830215
118	32	4.193051196316013
118	33	3.397532896047071
118	34	3.48878028491664
118	35	3.625337821883001
118	36	4.245518110467668
118	37	4.500805459830747
118	38	5.351811986691164
118	39	5.345886432776762
118	40	8.109217790086468
118	41	6.0471948823531605
118	42	5.249143015523519
118	43	-0.29165753435723474
118	44	0.3188779777134556
118	45	2.957097317860203
118	46	-1.1294727708109527
118	47	0.3904068040780082
118	48	-1.881463991455977
118	49	-3.124126173905971
118	50	0.568559841223756
118	51	2.3180564713615155
118	52	2.3426400000604524
118	53	2.1517216558129597
118	54	2.4374089567545987
118	55	3.6095196338461264
118	56	4.679648473393524
119	1	1.8084058953585185
119	2	4.230109680203945
119	3	3.9186514961103427
119	4	5.390501063452767
119	5	4.682172715823931
119	6	4.09770609128601
119	7	2.879313382061291
119	8	2.9743016653108967
119	9	3.0131788791224636
119	10	3.7442594664764997
119	11	-2.284294178733866
119	12	-10.423617578524336
119	13	-6.55778647122726
119	14	-8.705667135173329
119	15	-8.170980299389463
119	16	-9.514386771755198
119	17	-0.2487090640321803
119	18	-7.201354861850962
119	19	-0.5969984484305293
119	20	-1.6095656248445565
119	21	4.500126839052619
119	22	5.011668633366401
119	23	3.8616962228362546
119	24	4.030879662177103
119	25	4.4977170632195005
119	26	4.3776145869686545
119	27	4.499090934469834
119	28	4.391959094518501
119	29	4.793138898730568
119	30	5.419568817816174
119	31	3.432037557163415
119	32	3.1501590394532712
119	33	2.862300052909813
119	34	2.62940528491664
119	35	2.8165142924712327
119	36	4.226829384977474
119	37	4.103440263752315
119	38	4.601505614142145
119	39	5.955874177874801
119	40	8.549475143027642
119	41	5.908714490196299
119	42	4.993321937092148
119	43	-3.243250671612138
119	44	-1.090129375227722
119	45	4.12437672962491
119	46	-2.571568359046246
119	47	-1.8427427057259145
119	48	-3.832138011063819
119	49	-4.663035487631461
119	50	0.49717503730218837
119	51	5.6443432360673995
119	52	5.634919411825159
119	53	6.066550087185508
119	54	4.828646701852637
119	55	6.467669143650049
119	56	8.004403375354308
120	1	2.4730693568969784
120	2	4.805830834050099
120	3	4.58403611149496
120	4	5.827279909606617
120	5	4.764865023516236
120	6	4.9147733989783156
120	7	3.1201787666766734
120	8	3.3718978191570486
120	9	3.286496186814773
120	10	4.1161344664765025
120	11	-3.404967255656942
120	12	-12.838040655447411
120	13	-10.659709548150335
120	14	-10.538359442865637
120	15	-2.675787991697156
120	16	-8.145396387139815
120	17	0.38326208981397514
120	18	-8.142220246466348
120	19	-1.18666190996899
120	20	-1.2886521633060966
120	21	3.867674915975698
120	22	6.294360941058709
120	23	4.469869299759335
120	24	4.570062354484794
120	25	4.250120909373347
120	26	4.952374202353269
120	27	4.544523626777526
120	28	4.837872556056961
120	29	5.4727062064228775
120	30	5.741203433200791
120	31	4.79525871100957
120	32	4.043428270222499
120	33	4.993309668294426
120	34	4.596472592608951
120	35	4.858821984778928
120	36	5.030435154208245
120	37	4.959930648367699
120	38	5.3810729218344555
120	39	6.1642876394132635
120	40	7.384330912258413
120	41	-5.188400894419086
120	42	-2.7487453706001617
120	43	-3.7302699023813695
120	44	-15.279312067535415
120	45	-3.1732194242212444
120	46	-8.782866435969323
120	47	-6.403560013418222
120	48	-5.1463207033715115
120	49	-14.315679718400691
120	50	-4.305949962697813
120	51	-0.19868561008644647
120	52	-0.9569075112517638
120	53	0.040107779493201434
120	54	0.10196400954494678
120	55	0.8957941436500505
120	56	3.309211067661998
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1790.0
5	100.0
6	82.0
7	167.0
8	190.0
9	276.0
10	139.0
11	192.0
12	156.0
13	142.0
14	173.0
15	194.0
16	261.0
17	364.0
18	499.0
19	639.0
20	899.0
21	1108.0
22	1336.0
23	1558.0
24	1776.0
25	1954.0
26	2075.0
27	2166.0
28	2306.0
29	2572.0
30	2899.0
31	3203.0
32	3639.0
33	4024.0
34	4270.0
35	4688.0
36	5102.0
37	5630.0
38	5746.0
39	5077.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.7299422788717	0.14541569599216536	0.10980368881041058	0.014838336325731158
8	99.73736144703456	0.18399537043906636	0.05045034350748594	0.028192839018889203
9	99.4643360586411	0.2581870520677222	0.14689952962473848	0.1305773596664342
10	60.17538913537014	27.2327986586144	5.261674061104269	7.330138144911193
11	38.7844434881961	23.060258483818792	22.077960619055393	16.077337408929708
12	32.89362396688083	16.804415888890535	23.68198477586693	26.619975368361697
13	26.446366833350645	16.34739513005802	27.039900286379893	30.166337750211447
14	22.31389016663452	20.21129790927841	31.003219918982683	26.471592005104387
15	23.497585405115366	20.088833243903892	29.726345913074585	26.68723543790616
16	31.08352881788758	23.156753867624786	24.394987677049617	21.364729637438014
17	30.259584131083304	22.39488252667824	25.9079507843923	21.43758255784615
18	27.36782751917855	22.370275844672296	25.017435045182733	25.24446159096642
19	27.588510354041418	24.802196986565725	23.363764566169376	24.245528093223484
20	28.128853954621775	25.787135406606144	24.990713362357173	21.093297276414912
21	28.563797427032483	23.44902289555295	26.002700577211286	21.984479100203284
22	25.08272372501595	23.422313890166635	28.179484516196045	23.31547786862137
23	24.621251465285713	23.113676494591427	28.45696140548722	23.808110634635646
24	27.18288122514394	22.52923369145842	25.103876061019765	25.18400902237787
25	26.75926750363577	24.65052087970795	24.29881577775799	24.29139583889829
26	25.76340127369624	24.636669982037617	24.400635363626915	25.199293380639226
27	23.177539567063572	22.069958725539685	26.267185319357424	28.485316388039315
28	23.588069446505415	24.83009348568037	23.899688381065438	27.682148686748775
29	27.378935164541158	26.0530931359434	22.8588840334136	23.709087666101848
30	27.336975887872665	22.590272003800926	26.330324266539968	23.742427841786434
31	29.09530735211184	23.62797186192158	24.175595856460184	23.101124929506398
32	30.957964733123553	23.855607670842485	23.298996615804786	21.887430980229176
33	31.49333769069653	23.150967780270058	23.452517119980985	21.90317740905242
34	25.82755851649169	23.61924872854891	29.09615441810665	21.457038336852747
35	24.23134649963592	27.80972760911239	27.692330554441025	20.266595336810663
36	25.652522493096182	28.814918193425783	23.988181845176232	21.54437746830181
37	27.333254072048508	27.86826774462014	22.760373320651528	22.038104862679823
38	26.931544342962543	27.821081129324966	23.871716300240976	21.375658227471515
39	28.671162472891474	26.03160928077003	22.16214610379965	23.13508214253884
40	23.256161985004713	20.028134867781088	21.562083776058426	35.15361937115578
41	18.473009871875657	18.930596813394544	19.674738200258048	42.921655114471754
42	22.607867359240352	25.8554227626546	24.697495125690217	26.83921475241483
43	21.36672022853062	21.31167053502351	24.901803249419746	32.41980598702613
44	22.13696099174539	22.899842059778884	25.22871532020145	29.734481628274278
45	25.899869109947645	24.889933365064255	24.876546882436934	24.333650642551166
46	24.027442926451766	27.030687263743786	23.769979462452003	25.171890347352445
47	20.56972408650261	22.22818791946309	24.873974645786724	32.32811334824758
48	23.358130280487625	24.56234130696245	28.901064635395784	23.178463777154143
49	24.364296448392327	22.738026157211088	25.472778943296287	27.424898451100297
50	22.648549808970223	24.27193125901259	27.648029494402905	25.431489437614285
51	22.294178948935535	25.737680512133394	28.326633913949678	23.64150662498139
52	22.47773929330618	24.960235465505196	27.105290541243626	25.456734699945
53	21.67903952869066	24.617284317955278	30.096552955353555	23.607123198000508
54	21.990837151187005	23.814482061045993	29.777771166775747	24.41690962099125
55	22.971605452705518	23.101077445086016	26.988213584141914	26.939103518066553
56	23.09042497805941	24.894759546015738	27.035268567688576	24.979546908236276
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	0.0
6	0.5
7	1.0
8	14.5
9	28.0
10	71.5
11	115.0
12	167.0
13	229.5
14	240.0
15	228.0
16	216.0
17	211.0
18	206.0
19	222.0
20	238.0
21	238.5
22	259.0
23	279.0
24	301.0
25	323.0
26	369.5
27	416.0
28	493.5
29	675.5
30	780.0
31	802.5
32	825.0
33	936.0
34	1047.0
35	1149.5
36	1252.0
37	1364.0
38	1632.0
39	1788.0
40	1897.5
41	2007.0
42	2203.5
43	2400.0
44	2638.5
45	2877.0
46	2951.0
47	3127.0
48	3229.0
49	3391.5
50	3554.0
51	3637.0
52	3720.0
53	3895.5
54	4359.5
55	4648.0
56	4702.5
57	4757.0
58	5100.0
59	5443.0
60	5221.0
61	4999.0
62	4320.0
63	3266.5
64	2892.0
65	2522.5
66	2153.0
67	1810.0
68	1467.0
69	1148.5
70	830.0
71	738.5
72	535.5
73	424.0
74	311.0
75	198.0
76	142.5
77	87.0
78	65.0
79	29.5
80	16.0
81	9.5
82	3.0
83	2.0
84	1.0
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.4466339234045079
16	0.057869511670351516
17	0.023741338121169853
18	0.0
19	0.04154734171204725
20	0.13651269419672668
21	0.0
22	0.0
23	0.0
24	0.007419168162865579
25	0.010386835428011812
26	0.04451500897719348
27	0.057869511670351516
28	0.004451500897719347
29	0.17064086774590834
30	0.060837178935497754
31	0.016322169958304274
32	0.031160506284035435
33	0.10980368881041058
34	0.21812354398824804
35	0.1483833632573116
36	0.057869511670351516
37	0.1558025314201772
38	0.24780021663971036
39	0.10535218791269123
40	0.8487528378318223
41	1.0965530544715325
42	0.30270206104491565
43	0.26857388749573397
44	0.4139895834878993
45	0.23889721484427165
46	0.29528289288205006
47	0.5089549359725788
48	0.06825634709836334
49	0.27302538839345336
50	0.1869630377042126
51	0.33089490006380484
52	0.18102770317392014
53	0.2611547193328684
54	0.2448325493745641
55	0.2923152256169038
56	0.24631638300713724
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	67393.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.42495511403261
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.23664692692462	68.31718427729882
2	7.537515092278503	11.67183535382013
3	1.9394775676038252	4.504918908491979
4	0.8260027980605225	2.5581291825560517
5	0.3909618812165814	1.5135103052245782
6	0.25297533490484675	1.1751962369979079
7	0.1916479809885203	1.0386835428011811
8	0.11498878859311217	0.7122401436350957
9	0.08624159144483413	0.600952621192112
>10	0.3947948408363518	5.451604766073627
>50	0.019164798098852028	1.0831985517783744
>100	0.009582399049426014	1.3725461101301324
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	221	0.3279272327986586	No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	200	0.2967667265146232	No Hit
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	200	0.2967667265146232	No Hit
CAAAGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGGGCACTGACTGTTCTTCT	184	0.27302538839345336	No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	120	0.17806003590877392	No Hit
CAAAGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	95	0.140964195094446	No Hit
CAAAGTGGGGACTTAAGCCAGCACTGCTGTGCGGGGCTCTGGCTGCGCCTGGTCTG	90	0.13354502693158044	No Hit
CAAAGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	84	0.12464202513614174	No Hit
CAAAGTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	77	0.11425518970812994	No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	74	0.10980368881041058	No Hit
CAAAGTGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTAC	70	0.1038683542801181	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.001483833632573116	0.0	0.0	0.0	0.0
13	0.002967667265146232	0.0	0.0	0.0	0.0
14	0.004451500897719347	0.0	0.0	0.0	0.0
15	0.005935334530292464	0.0	0.0	0.0	0.0
16	0.005935334530292464	0.0	0.0	0.0	0.0
17	0.00741916816286558	0.0	0.0	0.0	0.0
18	0.00741916816286558	0.0	0.0	0.0	0.0
19	0.00741916816286558	0.0	0.0	0.0	0.0
20	0.00741916816286558	0.0	0.0	0.0	0.0
21	0.01038683542801181	0.0	0.0	0.0	0.0
22	0.01038683542801181	0.0	0.0	0.0	0.0
23	0.011870669060584928	0.0	0.0	0.0	0.0
24	0.013354502693158044	0.0	0.0	0.0	0.0
25	0.013354502693158044	0.0	0.0	0.0	0.0
26	0.023741338121169856	0.0	0.0	0.0	0.0
27	0.028192839018889203	0.0	0.0	0.0	0.0
28	0.02967667265146232	0.0	0.0	0.0	0.0
29	0.031160506284035435	0.0	0.0	0.0	0.0
30	0.03412817354918166	0.0	0.0	0.0	0.0
31	0.03561200718175478	0.0	0.0	0.0	0.0
32	0.03857967444690101	0.0	0.0	0.0	0.0
33	0.03857967444690101	0.0	0.0	0.0	0.0
34	0.040063508079474126	0.0	0.0	0.0	0.0
35	0.04303117534462036	0.0	0.0	0.0	0.0
36	0.044515008977193474	0.0	0.0	0.0	0.0
37	0.044515008977193474	0.0	0.0	0.0	0.0
38	0.04599884260976659	0.0	0.0	0.0	0.0
39	0.04748267624233971	0.0	0.0	0.0	0.0
40	0.050450343507485944	0.0	0.0	0.0	0.0
41	0.05490184440520529	0.0	0.0	0.0	0.0
42	0.05786951167035152	0.0	0.0	0.0	0.0
43	0.05786951167035152	0.0	0.0	0.0	0.0
44	0.06232101256807087	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGGAA	15	0.008177685	50.335083	40
GCCCGTC	15	0.008177685	50.335083	39
ATGCTTG	25	2.0038662E-5	50.25973	36
TATGCTG	25	2.0038662E-5	50.25973	36
GAGCGTC	15	0.008299639	50.147125	34
ATATGCT	35	5.0709787E-8	50.14712	34
CTCGTGC	15	0.008348797	50.072334	45
TCGTGCG	15	0.008348797	50.072334	46
GTCTCGT	15	0.008373458	50.035023	43
ATAACAA	15	0.008422941	49.960567	29
CGGAGCG	25	2.0760286E-5	49.960567	32
TGGTATC	15	0.008422941	49.960567	16
GCATATG	35	5.2225005E-8	49.960564	32
ACAAAAC	15	0.008472642	49.88633	23
GAAAGCT	15	0.008472642	49.88633	23
TCGTGAC	15	0.008472642	49.88633	22
TGGGGTA	65	0.0	49.849297	6
TGGGATC	210	0.0	49.849297	6
TGGGACT	115	0.0	49.849297	6
GGATAGC	15	0.008497573	49.849293	8
>>END_MODULE