FastQCFastQC Report
Fri 17 Jun 2016
SRR1526133_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526133_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13900
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT1591.143884892086331No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT1350.9712230215827338No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT1000.7194244604316548No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT830.5971223021582734No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT450.3237410071942446No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC300.2158273381294964No Hit
CAAAGTGGGTACCTGGTTCATCCTGCCCGTAGCCTATGCT230.16546762589928057No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCCTCTGCT210.1510791366906475No Hit
CAAAGTGGGTACCTGGTTCATCCTGCCCGTAGCATATGCT210.1510791366906475No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCCGTAGCATCTGCT210.1510791366906475No Hit
CAAAGTGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC200.14388489208633093No Hit
CAAAGTGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT190.1366906474820144No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC190.1366906474820144No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG170.1223021582733813No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCCGTCGCCTATGCT160.11510791366906474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTAG503.43789E-1034.2290926
CCCGTAG900.034.2290926
CTGCCAG552.3646862E-1134.2290923
GCCCGTA1000.034.2290925
ATCCCGC301.3517938E-534.10507220
TGGTTGA1550.034.10507214
TTCATCC359.678133E-734.10507217
GCGATGG251.8919121E-434.10507218
CCTGCCA552.5465852E-1134.10507222
GTTCATC359.678133E-734.10507216
GGTTCAT359.678133E-734.10507215
CTGGTTG1650.034.10507213
CTGGTTC454.976755E-934.10507213
GATCCTG1250.034.10507219
TCCCGCC301.3517938E-534.10507221
CGATGGC251.8919121E-434.10507219
AGCCTAT601.8189894E-1233.9819531
CCTATGC750.033.9819533
GCCTATG650.033.9819532
AGTAGCA359.959385E-733.9819528
Produced by FastQC (version 0.11.5)