##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526133_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13900
Sequences flagged as poor quality	0
Sequence length	40
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.45021582733813	30.0	4.0	31.0	4.0	33.0
2	10.546978417266187	4.0	4.0	15.0	4.0	25.0
3	10.45273381294964	4.0	4.0	15.0	4.0	24.0
4	11.38928057553957	4.0	4.0	17.0	4.0	28.0
5	12.246187050359712	4.0	4.0	20.0	4.0	29.0
6	10.827266187050359	4.0	4.0	16.0	4.0	27.0
7	13.566187050359712	4.0	4.0	23.0	4.0	31.0
8	12.497338129496402	4.0	4.0	21.0	4.0	29.0
9	13.422230215827339	4.0	4.0	23.0	4.0	31.0
10	10.733165467625899	4.0	4.0	16.0	4.0	27.0
11	14.175611510791366	4.0	4.0	27.0	4.0	32.0
12	15.097122302158274	4.0	4.0	29.0	4.0	33.0
13	14.924604316546763	4.0	4.0	29.0	4.0	33.0
14	15.16453237410072	4.0	4.0	30.0	4.0	33.0
15	14.398776978417267	4.0	4.0	29.0	4.0	33.0
16	13.06798561151079	4.0	4.0	26.0	4.0	32.0
17	13.022302158273382	4.0	4.0	26.0	4.0	32.0
18	12.807482014388489	4.0	4.0	26.0	4.0	32.0
19	11.47841726618705	4.0	4.0	20.0	4.0	31.0
20	11.544820143884893	4.0	4.0	22.0	4.0	32.0
21	10.167553956834533	4.0	4.0	15.0	4.0	30.0
22	10.318273381294963	4.0	4.0	15.0	4.0	30.0
23	10.123525179856115	4.0	4.0	14.0	4.0	31.0
24	9.459856115107913	4.0	4.0	7.0	4.0	30.0
25	9.098201438848921	4.0	4.0	4.0	4.0	30.0
26	8.132661870503597	4.0	4.0	4.0	4.0	27.0
27	8.073093525179855	4.0	4.0	4.0	4.0	27.0
28	7.391942446043165	4.0	4.0	4.0	4.0	23.0
29	7.091007194244605	4.0	4.0	4.0	4.0	21.0
30	6.635971223021583	4.0	4.0	4.0	4.0	17.0
31	6.590143884892086	4.0	4.0	4.0	4.0	16.0
32	6.390359712230216	4.0	4.0	4.0	4.0	15.0
33	6.416762589928058	4.0	4.0	4.0	4.0	13.0
34	6.282230215827338	4.0	4.0	4.0	4.0	7.0
35	6.157697841726619	4.0	4.0	4.0	4.0	4.0
36	6.152086330935251	4.0	4.0	4.0	4.0	4.0
37	6.044100719424461	4.0	4.0	4.0	4.0	4.0
38	5.9741726618705036	4.0	4.0	4.0	4.0	4.0
39	5.9890647482014385	4.0	4.0	4.0	4.0	4.0
40	5.930071942446043	4.0	4.0	4.0	4.0	4.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	11.690911021480371
1	2	15.987956071359362
1	3	5.545511474886824
1	4	18.05621173289385
1	5	13.955687936540672
1	6	14.048666058190282
1	7	16.72554270449251
1	8	11.96264844851904
1	9	13.778467017168246
1	10	10.766848295568394
1	11	6.591860151146694
1	12	7.407306810074926
1	13	1.7465606415292125
1	14	8.626656913430738
1	15	4.496020442100287
1	16	6.713430775900742
1	17	7.987891050575827
1	18	5.9006114605610325
1	19	3.100933088302151
1	20	8.881721272258925
1	21	10.39039304431797
1	22	10.237978979892262
1	23	9.127411178986092
1	24	10.945995644220984
1	25	8.11720758457934
1	26	4.737317284023117
1	27	7.912005356209159
1	28	6.859575123814118
1	29	6.59204414149611
1	30	4.987856867968541
1	31	8.128137946042212
1	32	8.261253527842182
1	33	8.939507594614145
1	34	6.410425694903495
1	35	7.096472721644398
1	36	4.897750942136745
1	37	5.377216590638075
1	38	5.206203019661399
1	39	6.262486702893845
1	40	5.7893192586747375
5	1	12.290911021480373
5	2	-5.11204392864064
5	3	-5.354488525113176
5	4	-5.643788267106148
5	5	-6.969312063459329
5	6	-5.42633394180972
5	7	-7.774457295507489
5	8	-6.937351551480958
5	9	-7.671532982831753
5	10	-5.5331517044316065
5	11	-8.558139848853305
5	12	-9.442693189925075
5	13	-9.353439358470787
5	14	-9.348343086569264
5	15	-8.678979557899714
5	16	-7.436569224099257
5	17	-7.312108949424173
5	18	-7.0993885394389675
5	19	-6.124066911697849
5	20	-5.993278727741075
5	21	-4.609606955682031
5	22	-4.837021020107738
5	23	-4.6475888210139065
5	24	-4.254004355779015
5	25	-3.8077924154206606
5	26	-3.1126827159768826
5	27	-2.9129946437908405
5	28	-2.1404248761858824
5	29	-2.3329558585038903
5	30	-2.162143132031459
5	31	-1.9718620539577865
5	32	-1.6137464721578167
5	33	-1.5604924053858547
5	34	-1.3645743050965056
5	35	-1.3035272783556024
5	36	-1.3272490578632548
5	37	-1.2977834093619256
5	38	-1.2437969803386002
5	39	-1.2875132971061554
5	40	-1.2606807413252632
6	1	-5.551194241677523
6	2	-1.7962544549564292
6	3	-1.9860674724815972
6	4	-3.433261951316674
6	5	-3.9166804845119607
6	6	-2.2684392049676143
6	7	-3.7744572955074887
6	8	-3.4636673409546423
6	9	-3.829427719673858
6	10	-2.4805201254842384
6	11	-5.084455638326989
6	12	-5.916377400451391
6	13	-5.0376498847865765
6	14	-4.874658876042949
6	15	-4.731611136847082
6	16	-4.015516592520309
6	17	-2.8910563178452264
6	18	-3.1520201183863357
6	19	-2.0188037538031125
6	20	-1.9932787277410746
6	21	-1.188554324103083
6	22	-0.8896525990551059
6	23	-2.0686414525928543
6	24	-1.5697938294632259
6	25	-1.070950310157503
6	26	-0.7442616633453039
6	27	-0.43931043326452457
6	28	0.3858909132878017
6	29	0.03546519412768845
6	30	-0.47793260571566965
6	31	0.3965589986737923
6	32	0.5441482646842886
6	33	1.228981278824672
6	34	1.2143730633245466
6	35	-0.6719483309871812
6	36	-0.3798806368106229
6	37	0.22853238011175847
6	38	-0.5069548750754427
6	39	0.0809077555254234
6	40	-1.2606807413252632
7	1	-1.081182001775442
7	2	-2.1585555565476167
7	3	-3.1451861995317802
7	4	-3.9228580345480077
7	5	-4.341405086715143
7	6	-2.496101383670185
7	7	-3.7512014815540002
7	8	-2.5420027142716553
7	9	-3.5785097270177992
7	10	-2.881988913733932
7	11	-0.13953519769051326
7	12	-0.9078094689948433
7	13	-1.0743695910289262
7	14	-0.766947737732055
7	15	-1.0045609532485518
7	16	0.19133775264492847
7	17	-0.6376903447730111
7	18	-0.09938853943896753
7	19	-0.6356948186745921
7	20	0.029977086212413795
7	21	0.5066721140854114
7	22	-0.06957915964262185
7	23	0.37566699293958195
7	24	-0.25400435577901526
7	25	-0.3194203223974048
7	26	-2.2754734136513015
7	27	-0.9827620856513057
7	28	-1.8846109226975107
7	29	-1.705048881759704
7	30	-1.5342361552872728
7	31	-1.9718620539577865
7	32	-1.6137464721578167
7	33	-1.5604924053858547
7	34	-1.3645743050965056
7	35	-1.3035272783556024
7	36	-1.3272490578632548
7	37	-1.2977834093619256
7	38	-1.2437969803386002
7	39	-1.2875132971061554
7	40	-1.2606807413252632
8	1	10.290911021480373
8	2	-5.11204392864064
8	3	-5.354488525113176
8	4	-5.643788267106148
8	5	-6.969312063459329
8	6	-5.42633394180972
8	7	-7.774457295507489
8	8	-6.937351551480958
8	9	-7.671532982831753
8	10	-5.5331517044316065
8	11	-8.558139848853305
8	12	-9.442693189925075
8	13	-9.353439358470787
8	14	-9.348343086569264
8	15	-8.678979557899714
8	16	-7.436569224099257
8	17	-7.312108949424173
8	18	-7.0993885394389675
8	19	-6.124066911697849
8	20	-5.993278727741075
8	21	-4.609606955682031
8	22	-4.837021020107738
8	23	-4.6475888210139065
8	24	-4.254004355779015
8	25	-3.8077924154206606
8	26	-3.1126827159768826
8	27	-2.9129946437908405
8	28	-2.1404248761858824
8	29	-2.3329558585038903
8	30	-2.162143132031459
8	31	-1.9718620539577865
8	32	-1.6137464721578167
8	33	-1.5604924053858547
8	34	-1.3645743050965056
8	35	-1.3035272783556024
8	36	-1.3272490578632548
8	37	-1.2977834093619256
8	38	-1.2437969803386002
8	39	-1.2875132971061554
8	40	-1.2606807413252632
9	1	-3.2090889785196275
9	2	-3.61204392864064
9	3	-3.854488525113176
9	4	-2.6437882671061477
9	5	-0.9693120634593289
9	6	-2.1763339418097196
9	7	-1.0244572955074887
9	8	-0.18735155148095828
9	9	-0.6715329828317529
9	10	-2.5331517044316065
9	11	4.191860151146695
9	12	-0.442693189925075
9	13	-0.3534393584707871
9	14	0.9016569134307364
9	15	4.571020442100286
9	16	3.313430775900743
9	17	4.187891050575827
9	18	4.1506114605610325
9	19	3.375933088302151
9	20	3.2567212722589254
9	21	6.140393044317969
9	22	0.9129789798922623
9	23	6.3524111789860935
9	24	6.495995644220985
9	25	7.442207584579339
9	26	3.3873172840231174
9	27	-2.9129946437908405
9	28	-2.1404248761858824
9	29	-2.3329558585038903
9	30	-2.162143132031459
9	31	-1.9718620539577865
9	32	-1.6137464721578167
9	33	-1.5604924053858547
9	34	-1.3645743050965056
9	35	-1.3035272783556024
9	36	-1.3272490578632548
9	37	-1.2977834093619256
9	38	-1.2437969803386002
9	39	-1.2875132971061554
9	40	-1.2606807413252632
10	1	-3.2090889785196275
10	2	-3.11204392864064
10	3	-1.854488525113176
10	4	-2.6437882671061477
10	5	-1.9693120634593289
10	6	-3.4263339418097196
10	7	-0.7744572955074887
10	8	-1.4373515514809583
10	9	-0.6715329828317529
10	10	-1.5331517044316065
10	11	-5.558139848853305
10	12	0.057306810074924996
10	13	-0.8534393584707871
10	14	3.1516569134307364
10	15	1.821020442100286
10	16	4.563430775900743
10	17	6.187891050575827
10	18	6.4006114605610325
10	19	8.375933088302151
10	20	8.506721272258925
10	21	8.39039304431797
10	22	9.662978979892262
10	23	9.852411178986094
10	24	9.245995644220985
10	25	10.69220758457934
10	26	10.387317284023117
10	27	-2.9129946437908405
10	28	-2.1404248761858824
10	29	-2.3329558585038903
10	30	-2.162143132031459
10	31	-1.9718620539577865
10	32	-1.6137464721578167
10	33	-1.5604924053858547
10	34	-1.3645743050965056
10	35	-1.3035272783556024
10	36	-1.3272490578632548
10	37	-1.2977834093619256
10	38	-1.2437969803386002
10	39	-1.2875132971061554
10	40	-1.2606807413252632
12	1	2.6242443548137047
12	2	-4.11204392864064
12	3	-4.021155191779843
12	4	-4.977121600439481
12	5	-4.969312063459329
12	6	-4.42633394180972
12	7	-4.774457295507489
12	8	-4.937351551480958
12	9	-2.671532982831753
12	10	-2.5331517044316065
12	11	-5.224806515519972
12	12	-3.109359856591741
12	13	-2.353439358470787
12	14	-2.0150097532359297
12	15	-1.34564622456638
12	16	1.2300974425674092
12	17	2.3545577172424927
12	18	2.5672781272276985
12	19	3.5425997549688173
12	20	2.6733879389255915
12	21	4.390393044317969
12	22	-4.837021020107738
12	23	-4.6475888210139065
12	24	-4.254004355779015
12	25	-3.8077924154206606
12	26	-3.1126827159768826
12	27	-2.9129946437908405
12	28	-2.1404248761858824
12	29	-2.3329558585038903
12	30	-2.162143132031459
12	31	-1.9718620539577865
12	32	-1.6137464721578167
12	33	-1.5604924053858547
12	34	-1.3645743050965056
12	35	-1.3035272783556024
12	36	-1.3272490578632548
12	37	-1.2977834093619256
12	38	-1.2437969803386002
12	39	-1.2875132971061554
12	40	-1.2606807413252632
13	1	12.235355465924815
13	2	14.221289404692692
13	3	18.423289252664603
13	4	10.41176728844941
13	5	16.08624349209623
13	6	18.24033272485695
13	7	14.558876037825843
13	8	17.507092892963485
13	9	17.439578128279358
13	10	15.189070517790615
13	11	16.052971262257806
13	12	14.723973476741593
13	13	17.92433841930699
13	14	17.70721246898629
13	15	15.321020442100286
13	16	16.007875220345184
13	17	18.299002161686936
13	18	20.17838923833881
13	19	15.26482197719104
13	20	18.228943494481147
13	21	17.168170822095746
13	22	13.71853453544782
13	23	17.296855623430538
13	24	17.912662310887654
13	25	15.69220758457934
13	26	9.553983950689785
13	27	9.031449800653604
13	28	9.026241790480784
13	29	13.555933030384999
13	30	12.115634645746319
13	31	8.583693501597768
13	32	4.941809083397739
13	33	5.661729816836367
13	34	4.079870139347939
13	35	5.640917166088842
13	36	7.450528719914524
13	37	6.75777214619363
13	38	6.3673141307725105
13	39	8.212486702893845
13	40	7.794874814230292
15	1	13.290911021480373
15	2	-5.11204392864064
15	3	-5.354488525113176
15	4	-5.643788267106148
15	5	-6.969312063459329
15	6	-5.42633394180972
15	7	-7.774457295507489
15	8	-6.937351551480958
15	9	-7.671532982831753
15	10	-5.5331517044316065
15	11	-8.558139848853305
15	12	-9.442693189925075
15	13	-9.353439358470787
15	14	-9.348343086569264
15	15	-8.678979557899714
15	16	-7.436569224099257
15	17	-7.312108949424173
15	18	-7.0993885394389675
15	19	-6.124066911697849
15	20	-5.993278727741075
15	21	-4.609606955682031
15	22	-4.837021020107738
15	23	-4.6475888210139065
15	24	-4.254004355779015
15	25	-3.8077924154206606
15	26	-3.1126827159768826
15	27	-2.9129946437908405
15	28	-2.1404248761858824
15	29	-2.3329558585038903
15	30	-2.162143132031459
15	31	-1.9718620539577865
15	32	-1.6137464721578167
15	33	-1.5604924053858547
15	34	-1.3645743050965056
15	35	-1.3035272783556024
15	36	-1.3272490578632548
15	37	-1.2977834093619256
15	38	-1.2437969803386002
15	39	-1.2875132971061554
15	40	-1.2606807413252632
16	1	3.213987944557296
16	2	-1.3428131594098707
16	3	-2.50833467895933
16	4	-3.2591728824907626
16	5	-4.20008129422856
16	6	-3.118641634117412
16	7	-3.4667649878151803
16	8	-3.0911977053271125
16	9	-3.5176868289855996
16	10	-2.3793055505854523
16	11	-3.3273706180840747
16	12	-2.211923959155845
16	13	-4.6611316661630955
16	14	-3.9637277019538786
16	15	-2.6020564809766373
16	16	-2.59041537794541
16	17	-1.8505704878857117
16	18	-2.637850077900506
16	19	-2.277913065544003
16	20	-2.0702018046641513
16	21	-0.5326838787589541
16	22	-2.8370210201077377
16	23	-4.6475888210139065
16	24	-4.254004355779015
16	25	-3.8077924154206606
16	26	-3.1126827159768826
16	27	-2.9129946437908405
16	28	-2.1404248761858824
16	29	-2.3329558585038903
16	30	-2.162143132031459
16	31	-1.9718620539577865
16	32	-1.6137464721578167
16	33	-1.5604924053858547
16	34	-1.3645743050965056
16	35	-1.3035272783556024
16	36	-1.3272490578632548
16	37	-1.2977834093619256
16	38	-1.2437969803386002
16	39	-1.2875132971061554
16	40	-1.2606807413252632
17	1	11.478411021480373
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107	25	-3.8077924154206606
107	26	-3.1126827159768826
107	27	-2.9129946437908405
107	28	-2.1404248761858824
107	29	-2.3329558585038903
107	30	-2.162143132031459
107	31	-1.9718620539577865
107	32	-1.6137464721578167
107	33	-1.5604924053858547
107	34	-1.3645743050965056
107	35	-1.3035272783556024
107	36	-1.3272490578632548
107	37	-1.2977834093619256
107	38	-1.2437969803386002
107	39	-1.2875132971061554
107	40	-1.2606807413252632
108	1	-4.375755645186294
108	2	-1.3898217064184175
108	3	-2.521155191779843
108	4	-3.0882327115505923
108	5	-2.802645396792663
108	6	-2.815222830698609
108	7	-1.7744572955074887
108	8	-0.7706848848142922
108	9	-1.4493107606095315
108	10	-0.6998183710982726
108	11	0.10852681781336138
108	12	0.3906401434082589
108	13	0.4798939748625468
108	14	0.42943469120851496
108	15	1.0987982198780646
108	16	0.6189863314562984
108	17	0.24344660613138203
108	18	-3.32161076166119
108	19	-1.0685113561422934
108	20	-2.6043898388521853
108	21	-2.3873847334598084
108	22	-3.2814654645521824
108	23	-2.258699932125017
108	24	-1.8095599113345706
108	25	0.46998536235711796
108	26	-1.668238271532438
108	27	-2.9129946437908405
108	28	-2.1404248761858824
108	29	-2.3329558585038903
108	30	-2.162143132031459
108	31	-1.9718620539577865
108	32	-1.6137464721578167
108	33	-1.5604924053858547
108	34	-1.3645743050965056
108	35	-1.3035272783556024
108	36	-1.3272490578632548
108	37	-1.2977834093619256
108	38	-1.2437969803386002
108	39	-1.2875132971061554
108	40	-1.2606807413252632
109	1	-15.709088978519627
109	2	-5.11204392864064
109	3	-5.354488525113176
109	4	-5.643788267106148
109	5	-6.969312063459329
109	6	-5.42633394180972
109	7	-7.774457295507489
109	8	-6.937351551480958
109	9	-7.671532982831753
109	10	-5.5331517044316065
109	11	-8.558139848853305
109	12	-9.442693189925075
109	13	-9.353439358470787
109	14	-9.348343086569264
109	15	-8.678979557899714
109	16	-7.436569224099257
109	17	-7.312108949424173
109	18	-7.0993885394389675
109	19	-6.124066911697849
109	20	-5.993278727741075
109	21	-4.609606955682031
109	22	-4.837021020107738
109	23	-4.6475888210139065
109	24	-4.254004355779015
109	25	-3.8077924154206606
109	26	-3.1126827159768826
109	27	-2.9129946437908405
109	28	-2.1404248761858824
109	29	-2.3329558585038903
109	30	-2.162143132031459
109	31	-1.9718620539577865
109	32	-1.6137464721578167
109	33	-1.5604924053858547
109	34	-1.3645743050965056
109	35	-1.3035272783556024
109	36	-1.3272490578632548
109	37	-1.2977834093619256
109	38	-1.2437969803386002
109	39	-1.2875132971061554
109	40	-1.2606807413252632
111	1	-15.709088978519627
111	2	-5.11204392864064
111	3	-5.354488525113176
111	4	-5.643788267106148
111	5	-6.969312063459329
111	6	-5.42633394180972
111	7	-7.774457295507489
111	8	-6.937351551480958
111	9	-7.671532982831753
111	10	-5.5331517044316065
111	11	-8.558139848853305
111	12	-9.442693189925075
111	13	-9.353439358470787
111	14	-9.348343086569264
111	15	-8.678979557899714
111	16	-7.436569224099257
111	17	-7.312108949424173
111	18	-7.0993885394389675
111	19	-6.124066911697849
111	20	-5.993278727741075
111	21	-4.609606955682031
111	22	-4.837021020107738
111	23	-4.6475888210139065
111	24	-4.254004355779015
111	25	-3.8077924154206606
111	26	-3.1126827159768826
111	27	-2.9129946437908405
111	28	-2.1404248761858824
111	29	-2.3329558585038903
111	30	-2.162143132031459
111	31	-1.9718620539577865
111	32	-1.6137464721578167
111	33	-1.5604924053858547
111	34	-1.3645743050965056
111	35	-1.3035272783556024
111	36	-1.3272490578632548
111	37	-1.2977834093619256
111	38	-1.2437969803386002
111	39	-1.2875132971061554
111	40	-1.2606807413252632
112	1	1.508302325828197
112	2	-0.329435232988466
112	3	-1.1805754816349143
112	4	0.7909943415895047
112	5	0.9002531539319758
112	6	-1.2089426374618935
112	7	2.008151400144685
112	8	-1.8503950297418275
112	9	1.8502061476030303
112	10	1.9016309042640458
112	11	2.311425368538
112	12	2.9920894187705773
112	13	4.037864989355299
112	14	2.4777438699524748
112	15	2.1905856594915907
112	16	-0.5235257458383877
112	17	0.9487606157932174
112	18	-0.27330158291722917
112	19	1.441150479606499
112	20	0.7458517070415347
112	21	-1.0878678252472485
112	22	0.11950071902269777
112	23	-1.386719255796515
112	24	-1.5148739209964068
112	25	0.23568584544890392
112	26	-0.851813150759491
112	27	-0.43473377422562276
112	28	-0.31433791966414315
112	29	-0.3764341193734557
112	30	0.05524817231636714
112	31	0.9411814243030827
112	32	0.5166883104508786
112	33	1.39602933374458
112	34	2.1571648253382767
112	35	1.739950982513963
112	36	1.759707463875876
112	37	2.5283035471598136
112	38	2.190985628357052
112	39	2.625530181154714
112	40	2.3914931717182153
114	1	-15.709088978519627
114	2	-5.11204392864064
114	3	-5.354488525113176
114	4	-5.643788267106148
114	5	-6.969312063459329
114	6	-5.42633394180972
114	7	-7.774457295507489
114	8	-6.937351551480958
114	9	-7.671532982831753
114	10	-5.5331517044316065
114	11	-8.558139848853305
114	12	-9.442693189925075
114	13	-9.353439358470787
114	14	-9.348343086569264
114	15	-8.678979557899714
114	16	-7.436569224099257
114	17	-7.312108949424173
114	18	-7.0993885394389675
114	19	-6.124066911697849
114	20	-5.993278727741075
114	21	-4.609606955682031
114	22	-4.837021020107738
114	23	-4.6475888210139065
114	24	-4.254004355779015
114	25	-3.8077924154206606
114	26	-3.1126827159768826
114	27	-2.9129946437908405
114	28	-2.1404248761858824
114	29	-2.3329558585038903
114	30	-2.162143132031459
114	31	-1.9718620539577865
114	32	-1.6137464721578167
114	33	-1.5604924053858547
114	34	-1.3645743050965056
114	35	-1.3035272783556024
114	36	-1.3272490578632548
114	37	-1.2977834093619256
114	38	-1.2437969803386002
114	39	-1.2875132971061554
114	40	-1.2606807413252632
116	1	11.290911021480373
116	2	-5.11204392864064
116	3	-5.354488525113176
116	4	-5.643788267106148
116	5	-6.969312063459329
116	6	-5.42633394180972
116	7	-7.774457295507489
116	8	-6.937351551480958
116	9	-7.671532982831753
116	10	-5.5331517044316065
116	11	-8.558139848853305
116	12	-9.442693189925075
116	13	-9.353439358470787
116	14	-9.348343086569264
116	15	-8.678979557899714
116	16	-7.436569224099257
116	17	-7.312108949424173
116	18	-7.0993885394389675
116	19	-6.124066911697849
116	20	-5.993278727741075
116	21	-4.609606955682031
116	22	-4.837021020107738
116	23	-4.6475888210139065
116	24	-4.254004355779015
116	25	-3.8077924154206606
116	26	-3.1126827159768826
116	27	-2.9129946437908405
116	28	-2.1404248761858824
116	29	-2.3329558585038903
116	30	-2.162143132031459
116	31	-1.9718620539577865
116	32	-1.6137464721578167
116	33	-1.5604924053858547
116	34	-1.3645743050965056
116	35	-1.3035272783556024
116	36	-1.3272490578632548
116	37	-1.2977834093619256
116	38	-1.2437969803386002
116	39	-1.2875132971061554
116	40	-1.2606807413252632
118	1	11.290911021480373
118	2	5.88795607135936
118	3	11.645511474886824
118	4	0.35621173289385233
118	5	15.030687936540671
118	6	10.57366605819028
118	7	21.22554270449251
118	8	22.06264844851904
118	9	18.328467017168247
118	10	23.466848295568393
118	11	18.441860151146695
118	12	20.557306810074927
118	13	-9.353439358470787
118	14	-9.348343086569264
118	15	-8.678979557899714
118	16	-7.436569224099257
118	17	-7.312108949424173
118	18	-7.0993885394389675
118	19	-6.124066911697849
118	20	-5.993278727741075
118	21	-4.609606955682031
118	22	-4.837021020107738
118	23	-4.6475888210139065
118	24	-4.254004355779015
118	25	-3.8077924154206606
118	26	-3.1126827159768826
118	27	-2.9129946437908405
118	28	-2.1404248761858824
118	29	-2.3329558585038903
118	30	-2.162143132031459
118	31	-1.9718620539577865
118	32	-1.6137464721578167
118	33	-1.5604924053858547
118	34	-1.3645743050965056
118	35	-1.3035272783556024
118	36	-1.3272490578632548
118	37	-1.2977834093619256
118	38	-1.2437969803386002
118	39	-1.2875132971061554
118	40	-1.2606807413252632
120	1	13.290911021480373
120	2	-5.11204392864064
120	3	-5.354488525113176
120	4	-5.643788267106148
120	5	-6.969312063459329
120	6	-5.42633394180972
120	7	-7.774457295507489
120	8	-6.937351551480958
120	9	-7.671532982831753
120	10	-5.5331517044316065
120	11	-8.558139848853305
120	12	-9.442693189925075
120	13	-9.353439358470787
120	14	-9.348343086569264
120	15	-8.678979557899714
120	16	-7.436569224099257
120	17	-7.312108949424173
120	18	-7.0993885394389675
120	19	-6.124066911697849
120	20	-5.993278727741075
120	21	-4.609606955682031
120	22	-4.837021020107738
120	23	-4.6475888210139065
120	24	-4.254004355779015
120	25	-3.8077924154206606
120	26	-3.1126827159768826
120	27	-2.9129946437908405
120	28	-2.1404248761858824
120	29	-2.3329558585038903
120	30	-2.162143132031459
120	31	-1.9718620539577865
120	32	-1.6137464721578167
120	33	-1.5604924053858547
120	34	-1.3645743050965056
120	35	-1.3035272783556024
120	36	-1.3272490578632548
120	37	-1.2977834093619256
120	38	-1.2437969803386002
120	39	-1.2875132971061554
120	40	-1.2606807413252632
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	7209.0
5	25.0
6	74.0
7	68.0
8	79.0
9	240.0
10	400.0
11	441.0
12	516.0
13	522.0
14	447.0
15	497.0
16	478.0
17	393.0
18	342.0
19	328.0
20	321.0
21	278.0
22	244.0
23	239.0
24	172.0
25	162.0
26	125.0
27	97.0
28	75.0
29	66.0
30	29.0
31	19.0
32	6.0
33	7.0
34	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.5749279538905	0.24495677233429394	0.11527377521613834	0.06484149855907781
8	98.10017271157167	0.5613126079447324	0.5253310305123776	0.8131836499712146
9	97.911866359447	1.1376728110599077	0.4608294930875576	0.4896313364055299
10	34.34532374100719	35.23741007194245	19.798561151079134	10.618705035971223
11	26.58992805755396	34.10071942446043	23.625899280575542	15.683453237410072
12	27.906474820143885	16.16546762589928	22.834532374100718	33.093525179856115
13	22.361320958342326	16.447226419166846	25.699690625224836	35.49176199726599
14	17.85611510791367	22.568345323741006	38.49640287769785	21.07913669064748
15	32.53237410071942	21.9568345323741	25.39568345323741	20.115107913669064
16	36.77242967119937	20.979926613425427	22.649111446866684	19.598532268508524
17	22.359712230215827	21.43884892086331	35.482014388489205	20.719424460431654
18	22.0	22.654676258992808	34.28776978417266	21.057553956834532
19	26.726878612716764	16.510115606936417	18.995664739884393	37.767341040462426
20	21.179856115107913	34.776978417266186	21.884892086330936	22.15827338129496
21	20.136690647482013	21.633093525179856	35.84892086330935	22.38129496402878
22	18.381294964028775	19.446043165467625	24.77697841726619	37.39568345323741
23	19.707871636206647	21.90243200460498	25.615196431141175	32.7744999280472
24	20.34105626708879	19.80860555475608	34.52295294286948	25.327385235285654
25	30.775880700237735	23.53576831640372	21.90764354153159	23.780707441826955
26	17.872402056629735	23.354334129915273	23.636758635672386	35.13650517778261
27	17.152313116051516	20.353982300884958	24.67803439096338	37.81567019210016
28	17.492984097287184	26.394185795495428	24.408145642944522	31.704684464272866
29	28.811360196064296	19.945217328623947	24.868449506235134	26.37497296907662
30	18.45654525784349	18.680129823296067	36.48755860079336	26.375766318067072
31	18.95693572819736	29.899733102503067	27.937675827742915	23.20565534155666
32	30.179175361588833	17.55774627617471	26.17831186587033	26.084766496366125
33	18.612849845312613	18.23872221023095	27.0523059212893	36.09612202316713
34	15.194244604316548	25.258992805755398	31.294964028776977	28.25179856115108
35	14.268240034537344	19.894948913512735	38.96963591883724	26.86717513311268
36	15.426680097855808	28.968196862857965	28.385379191250536	27.219743848035687
37	15.75801224342816	19.848757652142602	39.14296002880807	25.250270075621174
38	24.847089299848886	20.846225804130388	28.5817082823631	25.724976613657624
39	18.132105338897684	20.132393150093538	26.183623542955818	35.551877968052956
40	15.223021582733812	23.18705035971223	35.89208633093525	25.6978417266187
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	4.5
12	8.0
13	8.0
14	13.5
15	19.0
16	32.0
17	45.0
18	45.0
19	65.0
20	85.0
21	91.0
22	97.0
23	97.0
24	105.5
25	114.0
26	155.0
27	196.0
28	196.0
29	225.5
30	255.0
31	305.5
32	356.0
33	356.0
34	399.5
35	443.0
36	505.0
37	567.0
38	567.0
39	638.5
40	710.0
41	780.5
42	851.0
43	851.0
44	860.5
45	870.0
46	879.0
47	888.0
48	888.0
49	1005.0
50	1122.0
51	1269.5
52	1417.0
53	1417.0
54	1383.0
55	1349.0
56	1277.5
57	1206.0
58	1206.0
59	1104.5
60	1003.0
61	905.5
62	808.0
63	808.0
64	694.5
65	581.0
66	504.0
67	427.0
68	427.0
69	327.5
70	228.0
71	179.5
72	131.0
73	131.0
74	102.5
75	74.0
76	54.0
77	34.0
78	34.0
79	21.0
80	8.0
81	6.0
82	4.0
83	4.0
84	3.5
85	3.0
86	1.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.14388489208633093
8	0.028776978417266185
9	0.08633093525179857
10	0.0
11	0.0
12	0.0
13	0.007194244604316546
14	0.0
15	0.0
16	0.007194244604316546
17	0.0
18	0.0
19	0.4316546762589928
20	0.0
21	0.0
22	0.0
23	0.014388489208633093
24	0.014388489208633093
25	0.1366906474820144
26	0.6546762589928057
27	0.007194244604316546
28	0.02158273381294964
29	0.19424460431654675
30	0.2517985611510791
31	0.2661870503597122
32	0.02158273381294964
33	0.007194244604316546
34	0.0
35	0.014388489208633093
36	0.014388489208633093
37	0.1079136690647482
38	0.02158273381294964
39	0.014388489208633093
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	13900.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.63309352517985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.01461038961038	85.10071942446044
2	2.6866883116883113	4.7625899280575545
3	0.5032467532467533	1.3381294964028778
4	0.21915584415584413	0.776978417266187
5	0.15422077922077923	0.6834532374100719
6	0.10551948051948051	0.5611510791366906
7	0.048701298701298704	0.302158273381295
8	0.056818181818181816	0.4028776978417266
9	0.024350649350649352	0.19424460431654675
>10	0.15422077922077923	2.446043165467626
>50	0.016233766233766232	1.316546762589928
>100	0.016233766233766232	2.115107913669065
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT	159	1.143884892086331	No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCCGTAGCATATGCT	135	0.9712230215827338	No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCCGTAGCCTATGCT	100	0.7194244604316548	No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCCTATGCT	83	0.5971223021582734	No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCCGTAGCCTCTGCT	45	0.3237410071942446	No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	30	0.2158273381294964	No Hit
CAAAGTGGGTACCTGGTTCATCCTGCCCGTAGCCTATGCT	23	0.16546762589928057	No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCCTCTGCT	21	0.1510791366906475	No Hit
CAAAGTGGGTACCTGGTTCATCCTGCCCGTAGCATATGCT	21	0.1510791366906475	No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCCGTAGCATCTGCT	21	0.1510791366906475	No Hit
CAAAGTGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC	20	0.14388489208633093	No Hit
CAAAGTGGGTACCTGGTTCATCCTGCCAGTAGCATATGCT	19	0.1366906474820144	No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC	19	0.1366906474820144	No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	17	0.1223021582733813	No Hit
CAAAGTGGGTACCTGGTTGATCCTGCCCGTCGCCTATGCT	16	0.11510791366906474	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.007194244604316547	0.0	0.0	0.0	0.0
12	0.014388489208633094	0.0	0.0	0.0	0.0
13	0.014388489208633094	0.0	0.0	0.0	0.0
14	0.014388489208633094	0.0	0.0	0.0	0.0
15	0.014388489208633094	0.0	0.0	0.0	0.0
16	0.014388489208633094	0.0	0.0	0.0	0.0
17	0.014388489208633094	0.0	0.0	0.0	0.0
18	0.014388489208633094	0.0	0.0	0.0	0.0
19	0.014388489208633094	0.0	0.0	0.0	0.0
20	0.014388489208633094	0.0	0.0	0.0	0.0
21	0.014388489208633094	0.0	0.0	0.0	0.0
22	0.02158273381294964	0.0	0.0	0.0	0.0
23	0.02158273381294964	0.0	0.0	0.0	0.0
24	0.02158273381294964	0.0	0.0	0.0	0.0
25	0.02158273381294964	0.0	0.0	0.0	0.0
26	0.02158273381294964	0.0	0.0	0.0	0.0
27	0.02158273381294964	0.0	0.0	0.0	0.0
28	0.02877697841726619	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAGTAG	50	3.43789E-10	34.22909	26
CCCGTAG	90	0.0	34.22909	26
CTGCCAG	55	2.3646862E-11	34.22909	23
GCCCGTA	100	0.0	34.22909	25
ATCCCGC	30	1.3517938E-5	34.105072	20
TGGTTGA	155	0.0	34.105072	14
TTCATCC	35	9.678133E-7	34.105072	17
GCGATGG	25	1.8919121E-4	34.105072	18
CCTGCCA	55	2.5465852E-11	34.105072	22
GTTCATC	35	9.678133E-7	34.105072	16
GGTTCAT	35	9.678133E-7	34.105072	15
CTGGTTG	165	0.0	34.105072	13
CTGGTTC	45	4.976755E-9	34.105072	13
GATCCTG	125	0.0	34.105072	19
TCCCGCC	30	1.3517938E-5	34.105072	21
CGATGGC	25	1.8919121E-4	34.105072	19
AGCCTAT	60	1.8189894E-12	33.98195	31
CCTATGC	75	0.0	33.98195	33
GCCTATG	65	0.0	33.98195	32
AGTAGCA	35	9.959385E-7	33.98195	28
>>END_MODULE