FastQCFastQC Report
Fri 17 Jun 2016
SRR1526131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2543
Sequences flagged as poor quality0
Sequence length40
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC481.8875344081793157No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG140.5505308690523004No Hit
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG90.3539127015336217No Hit
CAAAGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT70.2752654345261502No Hit
CAAAGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA70.2752654345261502No Hit
CAAAGTGGGTGTGTGTGTGTGTGTGTGTGTCTGTTTGTGT70.2752654345261502No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGC70.2752654345261502No Hit
CAAAGTGGGAACATTTCCTGGGCCTTTCAGGAATACCACG60.23594180102241447No Hit
CAAAGTGGGATTCGTAGGAGTAAACATAACATTCTTCCCT60.23594180102241447No Hit
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAACG50.19661816751867872No Hit
CAAAGTGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGGG50.19661816751867872No Hit
CAAAGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.19661816751867872No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.19661816751867872No Hit
CAAAGTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG50.19661816751867872No Hit
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.15729453401494298No Hit
CAAAGTGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAG40.15729453401494298No Hit
CAAAGTGGGCTACTCAGACTACCCAGATGCTTACACCACA40.15729453401494298No Hit
CAAAGTGGGCCTCCACCGTGCTGTATCACACAGAGGTGGC40.15729453401494298No Hit
CAAAGTGGGAGCCTTGCTTATTTCAAATATTTAATCAATT40.15729453401494298No Hit
CAAAGTGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCG40.15729453401494298No Hit
CAAAGTGGGACCCTAGATGACACATGAGCAAAAGCCCACT40.15729453401494298No Hit
CAAAGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC30.11797090051120723No Hit
CAAAGTGGGGGATTTGGTACTTTTAAATGATATTTTTGGA30.11797090051120723No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAAC30.11797090051120723No Hit
CAAAGTGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAG30.11797090051120723No Hit
CAAAGTGGGATATTCGTAGGAGTAAACATAACATTCTTCC30.11797090051120723No Hit
CAAAGTGGGGGAGCTTGCTCTTGGGAGGTTTCCATATCCT30.11797090051120723No Hit
CAAAGTGGGGGGCTGGTGAGTTGGCTCAGTGGGTAAGAGC30.11797090051120723No Hit
CAAAGTGGGATCGCAATTCCTACCGGTGTCAAAGTATTTA30.11797090051120723No Hit
CAAAGTGGGGTGTTGAGCTTTTGTTTTTCTTCAGTGCTGG30.11797090051120723No Hit
CAAAGTGGGAGACTCAGAGGTCAAAGTTCTCATGCTTGGA30.11797090051120723No Hit
CAAAGTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAG30.11797090051120723No Hit
CAAAGTGGGGAACCCCGCCTGTTTACCAAAAACATCACCT30.11797090051120723No Hit
CAAAGTGGGCGAGCTTCTTCACCTATGCCAAGTACATTGT30.11797090051120723No Hit
CAAAGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA30.11797090051120723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGGG900.034.4081654
AGTGGGA900.034.4081654
GTGGGGG501.2369128E-1034.4081655
GTGGGCT251.3971509E-434.4081655
GTGGGAC251.3971509E-434.4081655
AAGTGGG2350.034.4081653
AAAGTGG2400.034.4081652
AGTGGGT200.002152710534.408164
AGTGGGC355.606944E-734.408164
TGGGGGG355.606944E-734.408166
GTGGGAT403.4588084E-834.408165
GTGGGAG200.002152710534.408165
TGGGATT200.002152710534.408166
CAAAGTG2450.033.7059561
TCAGTGG250.006428296727.52653126
Produced by FastQC (version 0.11.5)