SRR1526130_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526130_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21885
Sequences flagged as poor quality	0
Sequence length	56
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGAGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	522	2.385195339273475	No Hit
CGAGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	376	1.7180717386337676	No Hit
CGAGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	189	0.8636052090472925	No Hit
CGAGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	123	0.5620287868403016	No Hit
CGAGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	113	0.516335389536212	No Hit
CGAGTAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	87	0.3975325565455792	No Hit
CGAGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	67	0.30614576193740006	No Hit
CGAGTAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	58	0.2650217043637194	No Hit
CGAGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	50	0.2284669865204478	No Hit
CGAGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	48	0.21932830705962986	No Hit
CGAGTAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	43	0.1964816084075851	No Hit
CGAGTAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT	42	0.19191226867717615	No Hit
CGAGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	40	0.18277358921635825	No Hit
CGAGTAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	34	0.1553575508339045	No Hit
CGAGTAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	32	0.1462188713730866	No Hit
CGAGTAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	31	0.14164953164267763	No Hit
CGAGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	28	0.12794151245145077	No Hit
CGAGTAGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT	28	0.12794151245145077	No Hit
CGAGTAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	27	0.12337217272104181	No Hit
CGAGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	26	0.11880283299063284	No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	25	0.1142334932602239	No Hit
CGAGTAGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	24	0.10966415352981493	No Hit
CGAGTAGGGATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTGGTGGTGGTA	24	0.10966415352981493	No Hit
CGAGTAGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG	23	0.10509481379940598	No Hit
CGAGTAGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	22	0.10052547406899702	No Hit
CGAGTAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG	22	0.10052547406899702	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTGAA	25	1.95932E-5	50.258064	41
TGTCCAA	20	4.0362205E-4	50.142532	38
GTCCAAT	20	4.0362205E-4	50.142532	39
TCCAATC	20	4.0362205E-4	50.142532	40
TTTGTCC	20	4.0362205E-4	50.142532	36
TTGCTCA	20	4.0362205E-4	50.142532	37
ATCCCCC	15	0.008227841	50.14253	10
GGCGCAT	25	1.9863335E-5	50.14253	37
TTGGGCC	15	0.008227841	50.14253	34
GCGCATT	25	1.9863335E-5	50.14253	38
GACGAAA	15	0.008227841	50.14253	36
GTGACGA	15	0.008227841	50.14253	34
TGGGCGC	30	9.813521E-7	50.14253	35
GGGCGCA	30	9.813521E-7	50.14253	36
CGCATTG	25	1.9863335E-5	50.14253	39
GCCGATT	15	0.008227841	50.14253	38
GCATTGA	25	1.9863335E-5	50.14253	40
CCGATTG	15	0.008227841	50.14253	39
TGACGAA	15	0.008227841	50.14253	35
CGATTGA	15	0.008227841	50.14253	40

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content