##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526130_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21885
Sequences flagged as poor quality	0
Sequence length	56
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.44011880283299	38.0	36.0	39.0	32.0	40.0
2	35.27635366689513	38.0	34.0	39.0	27.0	40.0
3	34.812382910669406	38.0	33.0	39.0	27.0	40.0
4	34.897464016449625	38.0	34.0	39.0	25.0	40.0
5	33.49124971441627	37.0	31.0	39.0	20.0	40.0
6	33.20150788211104	38.0	32.0	39.0	15.0	40.0
7	35.322732465158786	38.0	35.0	39.0	28.0	40.0
8	35.33095727667352	38.0	35.0	39.0	28.0	40.0
9	35.26534155814485	38.0	35.0	39.0	27.0	40.0
10	34.195567740461506	38.0	33.0	39.0	23.0	40.0
11	34.20530043408728	38.0	33.0	39.0	23.0	40.0
12	33.20945853324195	38.0	31.0	39.0	19.0	40.0
13	32.473429289467674	37.0	29.0	39.0	17.0	40.0
14	33.28092300662554	38.0	31.0	39.0	20.0	40.0
15	31.889741832305234	36.0	28.0	39.0	15.0	40.0
16	32.77139593328764	37.0	30.0	39.0	19.0	40.0
17	31.699611606122915	36.0	28.0	39.0	15.0	40.0
18	31.276582133881654	36.0	27.0	39.0	15.0	40.0
19	32.46045236463331	36.0	29.0	39.0	18.0	40.0
20	33.09362577107608	37.0	30.0	39.0	21.0	40.0
21	33.56860863605209	38.0	32.0	39.0	20.0	40.0
22	32.860954992003656	38.0	30.0	39.0	17.0	40.0
23	33.576467900388394	38.0	32.0	39.0	20.0	40.0
24	32.80383824537354	37.0	30.0	39.0	17.0	40.0
25	33.394836646104636	38.0	31.0	39.0	20.0	40.0
26	33.61498743431574	38.0	33.0	39.0	20.0	40.0
27	33.949920036554715	38.0	33.0	39.0	22.0	40.0
28	33.963536668951335	38.0	33.0	39.0	23.0	40.0
29	33.389353438428145	38.0	32.0	39.0	19.0	40.0
30	33.26607265250171	38.0	32.0	39.0	19.0	40.0
31	33.99812657071053	38.0	33.0	39.0	23.0	40.0
32	33.91816312542838	38.0	33.0	39.0	22.0	40.0
33	33.67173863376742	38.0	33.0	39.0	22.0	40.0
34	33.466849440255885	38.0	33.0	39.0	20.0	40.0
35	33.599268905643136	38.0	33.0	39.0	21.0	40.0
36	32.73552661640393	36.0	32.0	39.0	19.0	40.0
37	32.534932602238975	36.0	31.0	39.0	17.0	40.0
38	31.983321909984006	36.0	31.0	39.0	11.0	40.0
39	30.859812657071053	35.0	29.0	38.0	4.0	40.0
40	28.51464473383596	34.0	21.0	38.0	4.0	40.0
41	26.354580763079735	31.0	16.0	38.0	4.0	40.0
42	27.44290610006854	32.0	21.0	38.0	4.0	39.0
43	27.674800091386796	33.0	22.0	38.0	4.0	40.0
44	26.945853324194655	32.0	19.0	38.0	4.0	40.0
45	27.764679003883938	33.0	22.0	38.0	4.0	40.0
46	25.80657984921179	30.0	18.0	36.0	4.0	39.0
47	24.917249257482293	29.0	13.0	36.0	4.0	39.0
48	25.446881425633997	30.0	17.0	36.0	4.0	39.0
49	24.459355723098014	29.0	11.0	36.0	4.0	39.0
50	25.223532099611607	30.0	17.0	36.0	4.0	39.0
51	25.506785469499658	31.0	15.0	36.0	4.0	39.0
52	25.31574137537126	30.0	11.0	36.0	4.0	39.0
53	25.265752798720584	31.0	10.0	36.0	4.0	39.0
54	24.816815170207907	30.0	4.0	36.0	4.0	39.0
55	24.666666666666668	30.0	4.0	36.0	4.0	39.0
56	23.907013936486177	30.0	4.0	36.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	3.1436711170325893
1	2	4.063221198057185
1	3	4.2831898708974165
1	4	4.574033266372581
1	5	5.529278424603248
1	6	7.198574666954347
1	7	4.425892126763394
1	8	4.178573423076578
1	9	4.399139173356488
1	10	4.981182975169993
1	11	1.7865086445991167
1	12	-4.612247860307587
1	13	-15.447863958897534
1	14	-4.303692758271733
1	15	1.8695885909455896
1	16	-2.699822486111781
1	17	-4.300660395158612
1	18	-3.968953033042226
1	19	0.483618409309333
1	20	-0.948533129982053
1	21	5.193707578720808
1	22	5.588028875059003
1	23	5.217822606998748
1	24	5.055756386818974
1	25	5.930743243172358
1	26	5.684788199482568
1	27	4.1204728120864615
1	28	3.9883783452567343
1	29	5.164636319092924
1	30	4.339778850261634
1	31	2.508287409483465
1	32	3.7021599430240997
1	33	2.3257443049566398
1	34	4.045771639854436
1	35	3.471136357154222
1	36	1.5173021801864373
1	37	1.0777467117006552
1	38	2.2689006701651167
1	39	0.5981923756982361
1	40	-12.439117269633876
1	41	-1.5857030074655398
1	42	-3.784237992529775
1	43	-11.042344914586415
1	44	-13.468257134617318
1	45	-3.2571171504775513
1	46	-7.028613944321595
1	47	-11.77001552802187
1	48	-6.9278858836546995
1	49	-14.373247999922764
1	50	-5.880473063803581
1	51	-1.4486169024458242
1	52	-1.761982227356519
1	53	-1.1927181597635723
1	54	-1.4233342761813148
1	55	-1.9613560020802403
1	56	1.9459087101000136
2	1	3.4556140760343723
2	2	3.390315672210484
2	3	3.6762379992396674
2	4	4.133748061381496
2	5	5.843895180396473
2	6	7.397326895118336
2	7	4.240508882556618
2	8	4.204420125393867
2	9	3.6308682286149505
2	10	5.023963723833091
2	11	4.804333956542081
2	12	1.4483582002984754
2	13	1.7455406756835714
2	14	5.922688703403846
2	15	6.6663800347958535
2	16	3.9062381199488243
2	17	6.863332474716607
2	18	3.4927225462804117
2	19	6.036203079541064
2	20	4.976600559857516
2	21	6.194598844317955
2	22	6.954339035486811
2	23	6.333687134627983
2	24	5.940783124786893
2	25	5.649994580070754
2	26	5.965536862584173
2	27	5.8789398352593665
2	28	5.540963015488465
2	29	5.792978565082233
2	30	5.820171007124383
2	31	4.923617177754416
2	32	4.376848000065095
2	33	4.963890472514567
2	34	4.282848288695796
2	35	5.136911758223739
2	36	7.040475085712284
2	37	7.063486462146287
2	38	7.030041490129463
2	39	7.566106814200914
2	40	4.830044940704806
2	41	9.785063480948008
2	42	7.852125643833862
2	43	6.770489310012518
2	44	-3.328328435865089
2	45	7.471046842392322
2	46	6.692419923771098
2	47	3.937649356113603
2	48	6.00348666536491
2	49	-0.17182197496732954
2	50	6.524161517301589
2	51	11.913236929996248
2	52	10.113240589042768
2	53	10.489991287651755
2	54	11.228180875333837
2	55	9.8238489890071
2	56	12.22398357641017
3	1	3.833065056426534
3	2	5.128877763713753
3	3	4.507937345644898
3	4	5.5765585188978335
3	5	6.754025899350722
3	6	7.622817091196769
3	7	4.650639601510868
3	8	4.612916857420011
3	9	5.000149274366585
3	10	6.241283985271004
3	11	5.864791472881947
3	12	6.11502486696514
3	13	7.211226950193378
3	14	6.198832494253519
3	15	2.4049421262991224
3	16	6.184015897726603
3	17	7.663986069487848
3	18	8.033572219483027
3	19	6.688163863854786
3	20	5.7560123245634
3	21	6.474010609023839
3	22	7.542574329604456
3	23	6.2961054352815715
3	24	7.285554366616953
3	25	6.607510919940033
3	26	5.508020522714894
3	27	5.880573822187472
3	28	4.998479355357745
3	29	5.3893837938404054
3	30	5.67311218359497
3	31	4.296166197362254
3	32	3.530442771306923
3	33	4.1893806685929995
3	34	3.2452665893493844
3	35	5.190833326851191
3	36	6.360736523620783
3	37	6.620676004629949
3	38	7.092132993397435
3	39	6.757283284789146
3	40	8.851286770770162
3	41	10.353690931928401
3	42	7.907681199389419
3	43	7.9551298328883355
3	44	9.53931862295844
3	45	7.889347495987092
3	46	8.264315348607699
3	47	9.651701643695304
3	48	9.072114116345304
3	49	9.891903515228748
3	50	8.07154713821662
3	51	9.826635622806702
3	52	9.190037974663682
3	53	9.24162527457986
3	54	9.67262531977828
3	55	10.513391472667234
3	56	8.789343053534356
4	1	3.449731723093201
4	2	4.551099985935977
4	3	4.780159567867116
4	4	4.971002963342279
4	5	6.192914788239612
4	6	7.489483757863436
4	7	4.922861823733086
4	8	3.6240279685311236
4	9	4.811260385477695
4	10	6.202395096382112
4	11	6.325902583993056
4	12	7.015024866965145
4	13	6.961226950193378
4	14	6.159943605364628
4	15	5.39383101518801
4	16	6.839571453282154
4	17	8.108430513932298
4	18	9.428016663927473
4	19	7.804830530521453
4	20	7.039345657896732
4	21	7.4573439423571735
4	22	6.642574329604457
4	23	6.057216546392684
4	24	7.813332144394732
4	25	5.718622031051147
4	26	6.130242744937114
4	27	4.741684933298586
4	28	5.909590466468856
4	29	5.861606016062627
4	30	6.506445516928306
4	31	4.962832864028925
4	32	4.241553882418039
4	33	5.456047335259665
4	34	4.373044367127164
4	35	4.207499993517857
4	36	6.671847634731893
4	37	5.992898226852169
4	38	7.314355215619656
4	39	6.607283284789148
4	40	0.9123978818812759
4	41	11.720357598595069
4	42	10.685458977167198
4	43	9.99401872177722
4	44	11.389318622958442
4	45	9.900458607098205
4	46	12.786537570829918
4	47	13.390590532584191
4	48	12.038780783011966
4	49	12.008570181895415
4	50	12.343769360438845
4	51	11.621080067251146
4	52	11.162260196885903
4	53	12.11940305235764
4	54	11.92818087533384
4	55	11.602280361556122
4	56	10.200454164645468
5	1	2.8037857771472545
5	2	4.535784670620664
5	3	4.025204612912162
5	4	4.9556876480269665
5	5	6.712734608059428
5	6	7.658853127232806
5	7	4.366105066976331
5	8	4.300604545107696
5	9	4.359008133225444
5	10	5.651944645931664
5	11	5.608785466875943
5	12	6.232141984082258
5	13	6.7495152384816635
5	14	6.15003369545472
5	15	7.825362546719543
5	16	6.933265146975849
5	17	8.302124207625987
5	18	8.700989636900445
5	19	7.082308007998936
5	20	6.830336648887723
5	21	6.169956554969783
5	22	6.339871626901754
5	23	5.64370303287917
5	24	7.173692504755092
5	25	4.709613022042134
5	26	4.851864366558736
5	27	5.043486735100387
5	28	5.560040916919306
5	29	5.122867277323888
5	30	4.916355426838212
5	31	3.926796827992888
5	32	3.4523646932288514
5	33	4.184876164088497
5	34	4.322593916676716
5	35	3.1615540475719115
5	36	6.79166745455171
5	37	5.160465794419736
5	38	6.931472332736774
5	39	8.182958960464823
5	40	8.334019503502898
5	41	11.50684408508155
5	42	10.122395914104136
5	43	9.594018721777221
5	44	10.559588893228714
5	45	8.040999147638747
5	46	11.783834868127219
5	47	11.182482424476085
5	48	11.063105107336291
5	49	12.286047659372898
5	50	11.444670261339741
5	51	11.178737724908803
5	52	9.644242178867884
5	53	10.289673322627912
5	54	8.917370064523027
5	55	9.465343424619185
5	56	9.886940651131955
6	1	3.9163983897598627
6	2	5.0844333192693085
6	3	5.346826234533786
6	4	5.6376696300089435
6	5	7.126248121572942
6	6	7.72281709119677
6	7	4.322861823733092
6	8	4.490694635197784
6	9	4.311260385477695
6	10	5.702395096382112
6	11	5.55923591732639
6	12	6.18169153363181
6	13	6.894560283526708
6	14	7.193276938697959
6	15	8.46049768185468
6	16	6.939571453282156
6	17	7.475097180598965
6	18	8.628016663927468
6	19	7.771497197188122
6	20	6.339345657896736
6	21	6.824010609023841
6	22	7.009240996271124
6	23	6.590549879726016
6	24	7.046665477728066
6	25	5.685288697717809
6	26	6.163576078270445
6	27	4.208351599965248
6	28	5.542923799802189
6	29	6.294939349395957
6	30	5.906445516928304
6	31	4.529499530695588
6	32	4.908220549084703
6	33	4.956047335259665
6	34	4.106377700460499
6	35	6.4741666601845225
6	36	7.371847634731896
6	37	7.192898226852172
6	38	7.014355215619659
6	39	8.973949951455815
6	40	6.612397881881275
6	41	12.287024265261731
6	42	9.185458977167198
6	43	10.627352055110553
6	44	10.955985289625104
6	45	9.70045860709821
6	46	12.386537570829919
6	47	13.79059053258419
6	48	12.638780783011967
6	49	11.475236848562083
6	50	13.177102693772174
6	51	10.321080067251149
6	52	9.462260196885907
6	53	9.086069719024309
6	54	10.461514208667172
6	55	9.402280361556123
6	56	9.667120831312133
7	1	2.5438493701520244
7	2	3.949139201622252
7	3	4.264473293357312
7	4	4.310218649616786
7	5	5.69683635686706
7	6	7.2208563068830465
7	7	4.034626529615444
7	8	4.586773066570338
7	9	4.5132211697914215
7	10	5.2298460767742725
7	11	5.274922191836197
7	12	5.801299376769062
7	13	6.892599499212984
7	14	6.099159291639136
7	15	7.8428506230311505
7	16	6.259179296419411
7	17	7.892744239422491
7	18	8.875075487456883
7	19	7.242085432482238
7	20	6.535424089269284
7	21	5.518128256082665
7	22	5.983750800192688
7	23	4.539569487569157
7	24	5.411371360081009
7	25	4.94411222712958
7	26	4.230242744937115
7	27	4.3495280705534825
7	28	4.040963015488465
7	29	5.263566800376349
7	30	4.467229830653796
7	31	3.6883230601073578
7	32	3.2297891765356823
7	33	4.140361060749864
7	34	2.6063777004604987
7	35	2.9016176405766814
7	36	5.128710379829929
7	37	4.887015873910997
7	38	5.647688548952992
7	39	5.183753873024443
7	40	4.947691999528335
7	41	10.285063480948008
7	42	8.558007996775036
7	43	7.71166578060075
7	44	9.318730387664324
7	45	8.353399783568793
7	46	10.545361100241688
7	47	11.496472885525364
7	48	10.062310194776671
7	49	12.357589789738554
7	50	10.318279164360408
7	51	9.442648694702125
7	52	9.907358236101594
7	53	9.46057952294587
7	54	9.404651463569131
7	55	8.470907812536513
7	56	9.253395341116054
8	1	3.6026728995637853
8	2	4.68443331926931
8	3	4.293885058063196
8	4	5.016101002557967
8	5	6.402718709808241
8	6	7.397326895118336
8	7	4.299332411968386
8	8	4.763243654805628
8	9	4.6602799933208345
8	10	5.523963723833091
8	11	4.863157485953842
8	12	5.330711141474946
8	13	6.127893616860042
8	14	5.363865173992078
8	15	6.519321211266448
8	16	5.406238119948824
8	17	6.157450121775433
8	18	7.845663722750999
8	19	5.977379550129296
8	20	5.564835853975168
8	21	5.371069432553252
8	22	6.24845668254563
8	23	5.392510664039744
8	24	6.734900771845712
8	25	5.62058281536487
8	26	5.730242744937115
8	27	4.2024692470240765
8	28	4.864492427253168
8	29	4.64591974155282
8	30	5.702523948300854
8	31	3.4236171777544158
8	32	4.46508329418274
8	33	3.9344787078086902
8	34	2.9593188769310856
8	35	2.6369117582237394
8	36	4.187533909241697
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116	26	4.999473514167882
116	27	4.600659292272944
116	28	3.9685648254432166
116	29	4.528272682729291
116	30	5.288496798979583
116	31	4.09103799223405
116	32	5.103092343956497
116	33	5.291944771157102
116	34	5.414070008152805
116	35	5.107499993517855
116	36	7.189796352680613
116	37	5.054436688390631
116	38	5.4169193181837585
116	39	7.314975592481453
116	40	9.82778249726589
116	41	8.943434521671985
116	42	5.544433336141555
116	43	-0.7136735859150853
116	44	3.0970109306507467
116	45	6.6927662994059
116	46	1.9480760323683803
116	47	-6.24787100587735
116	48	-2.2227576785264915
116	49	0.3213906947159302
116	50	3.3771026937721764
116	51	5.08518263135371
116	52	5.226362760988469
116	53	4.904018436973022
116	54	4.574334721487684
116	55	5.812536771812532
116	56	9.746608010799314
117	1	1.8925888659503443
117	2	3.232052366888361
117	3	3.7896833773909293
117	4	3.604336296675612
117	5	4.750057645382469
117	6	5.622817091196769
117	7	3.0276237284949943
117	8	3.1049803494835047
117	9	3.4684032426205533
117	10	4.4833474773344975
117	11	1.4878073458978207
117	12	-7.789737037796762
117	13	-8.44353495456853
117	14	-1.2257706803496582
117	15	-7.753788032431036
117	16	-7.141380927670223
117	17	-0.10585520035341744
117	18	-2.033888097977293
117	19	0.12864005433097958
117	20	0.17267899123006814
117	21	4.128772513785744
117	22	5.137812424842551
117	23	4.204835594011737
117	24	4.718094049156637
117	25	3.861479173908286
117	26	4.5159570306514
117	27	2.8131135047271556
117	28	3.3000666569450487
117	29	4.1949393493959555
117	30	4.67311218359497
117	31	2.6771185783146407
117	32	4.022506263370417
117	33	4.022714001926335
117	34	4.106377700460499
117	35	4.726547612565476
117	36	5.495657158541416
117	37	4.97385060780455
117	38	4.814355215619656
117	39	5.935854713360577
117	40	8.379064548547941
117	41	4.7822623604998284
117	42	3.756887548595767
117	43	-6.5964574686989685
117	44	-8.71068137704156
117	45	3.8337919404315386
117	46	-1.8944148101224592
117	47	-2.4236951817015253
117	48	-0.5469335027023163
117	49	-2.381906008580774
117	50	0.7342455509150341
117	51	5.473461019632101
117	52	5.995593530219239
117	53	5.543212576167164
117	54	7.06151420866717
117	55	5.973708932984692
117	56	9.733787497978799
118	1	2.2234159336195134
118	2	3.28969647716405
118	3	3.857352550323256
118	4	4.025388928254557
118	5	4.173616542625574
118	6	5.640360950845892
118	7	3.228124981627829
118	8	2.952098143969714
118	9	3.3831902100391034
118	10	3.8041494823470288
118	11	0.23467451381762316
118	12	-5.183220747069946
118	13	-2.8721063831399576
118	14	-2.8172493770915104
118	15	-11.46055494972427
118	16	-4.4621829326827545
118	17	0.38036033849369844
118	18	-3.7982991255462153
118	19	-0.4127133291276692
118	20	0.8218017982476127
118	21	3.697694819550154
118	22	4.989942750657086
118	23	3.8414270727084734
118	24	3.30456021457017
118	25	5.069499224033599
118	26	5.440769060726588
118	27	4.697825284175778
118	28	3.828888712082893
118	29	4.791430577466134
118	30	5.409954288858128
118	31	3.945289004379802
118	32	4.95734335610225
118	33	4.338503475610548
118	34	5.527430332039444
118	35	4.212763151412595
118	36	6.410444125959966
118	37	6.329740332115328
118	38	5.989793812110889
118	39	9.138862232157564
118	40	10.238713671354962
118	41	8.757199703858223
118	42	4.957388801728598
118	43	1.6992818796719575
118	44	1.131423886116334
118	45	8.237300712361364
118	46	-1.9345150607490282
118	47	-0.8146726253105463
118	48	-0.7349034275143467
118	49	-3.0510789409116015
118	50	2.5613132200879676
118	51	4.90704497953185
118	52	5.3113830039034475
118	53	4.9141398944629024
118	54	4.517654559544365
118	55	6.26192948436314
118	56	10.189927848855994
119	1	2.5224589958204717
119	2	3.138978773814763
119	3	3.3286444163519633
119	4	3.604336296675612
119	5	4.408066303391124
119	6	6.001604969984651
119	7	3.304680005551269
119	8	2.693724938228087
119	9	3.18701796123527
119	10	3.5720920660790796
119	11	1.7562056142960927
119	12	-1.824369072428798
119	13	0.5824390714054957
119	14	-5.1521776067565845
119	15	-4.675865954508957
119	16	-3.4119436982329923
119	17	-0.5885391830374012
119	18	-5.68107424516344
119	19	-0.7285028028118781
119	20	-1.948533129982053
119	21	2.3603742453874723
119	22	3.2243925114226357
119	23	2.793580182756319
119	24	1.1012109322735206
119	25	2.658015970445085
119	26	2.8666063813007483
119	27	3.681078872692524
119	28	1.8671662240446167
119	29	2.4373635918201977
119	30	3.536748547231337
119	31	1.402226803422863
119	32	1.7779175187816705
119	33	3.2954412746536086
119	34	2.560923155005952
119	35	3.334772720790582
119	36	2.4415446044288593
119	37	2.6383527723067175
119	38	2.4204158216802654
119	39	4.325465102970966
119	40	5.39421606369946
119	41	5.702175780413249
119	42	4.761216552924772
119	43	-4.996890369131869
119	44	-0.8015904679506498
119	45	2.545913152552753
119	46	-2.0134624291700796
119	47	-2.70940946741581
119	48	-8.488491944260758
119	49	-1.0247631514379165
119	50	-0.5319882153187336
119	51	4.081686127857207
119	52	3.677411712037422
119	53	4.110312143266729
119	54	2.909999057152021
119	55	2.5992500585258185
119	56	5.08227234646365
120	1	3.178303151664629
120	2	4.136814271650259
120	3	4.884921472629024
120	4	4.937669630008948
120	5	5.750057645382469
120	6	6.86091232929201
120	7	4.075242776114038
120	8	4.2002184447215996
120	9	4.2779270521443635
120	10	5.578585572572592
120	11	3.05923591732639
120	12	0.21026296220323815
120	13	0.9850364740029001
120	14	-1.7495802041591801
120	15	3.7224024437594387
120	16	-9.236619022908318
120	17	0.037001942503724905
120	18	-1.3672214313106252
120	19	-1.4904075647166408
120	20	0.029821848372925785
120	21	2.8906772756905035
120	22	5.185431472461602
120	23	3.3953117844879266
120	24	3.432379763442352
120	25	3.3376696500987606
120	26	2.8492903639847356
120	27	4.09882779044144
120	28	3.3953047521831436
120	29	4.623510777967386
120	30	4.101683612166401
120	31	2.486642387838444
120	32	1.4510776919418475
120	33	2.6893806685929995
120	34	2.677806271889068
120	35	2.297976183994045
120	36	2.400419063303321
120	37	2.211945845899791
120	38	3.195307596572043
120	39	3.9834737609796207
120	40	5.712397881881277
120	41	-5.122499544262077
120	42	-4.766921975213759
120	43	-6.215505087746589
120	44	-15.329728996089178
120	45	-3.451922345282746
120	46	-9.418224333931985
120	47	-8.804647562653905
120	48	-5.308838264607079
120	49	-16.762858389533154
120	50	-4.360992544323061
120	51	-0.288443742272662
120	52	-2.528215993590287
120	53	-1.837739804785219
120	54	-1.7003905532375896
120	55	-1.3120053527295923
120	56	1.9718827360740399
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	602.0
5	46.0
6	66.0
7	54.0
8	34.0
9	39.0
10	38.0
11	40.0
12	44.0
13	51.0
14	53.0
15	68.0
16	102.0
17	126.0
18	160.0
19	197.0
20	261.0
21	394.0
22	415.0
23	561.0
24	575.0
25	653.0
26	685.0
27	755.0
28	740.0
29	735.0
30	871.0
31	970.0
32	1102.0
33	1207.0
34	1373.0
35	1488.0
36	1586.0
37	1824.0
38	1911.0
39	2057.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.42883253369888	0.2787297235549463	0.26045236463331045	0.03198537811286269
8	99.6344528215673	0.2513136851724926	0.07767877541695226	0.03655471784327165
9	98.1310546518004	0.6397367940047524	0.7311277645768598	0.49808078961798574
10	39.75782499428833	29.330591729495087	24.002741603838246	6.90884167237834
11	24.742974640164498	22.175005711674665	22.26639250628284	30.815627141878
12	26.228010052547408	30.011423349326023	19.42426319396847	24.336303404158098
13	19.922321224583047	13.328763993602925	21.425633995887594	45.323280785926435
14	19.05414667580535	37.692483436143476	22.84669865204478	20.406671236006396
15	39.42678669851185	18.027742054014332	19.3000183722212	23.245452875252617
16	25.482307762640577	35.745634086129655	21.21696991862485	17.555088232604916
17	24.258489100132536	18.362963301494446	39.34920707463096	18.02934052374206
18	21.03724011880283	17.623943340187342	23.171121772903817	38.16769476810601
19	20.45620771621869	19.72024136039495	22.316694093984275	37.50685682940208
20	20.384351407000686	21.67467398764585	37.40562800274537	20.535346602608097
21	21.987662782727895	17.957505140507195	24.180945853324197	35.873886223440714
22	19.739547635366687	18.437285812200138	38.98560657984921	22.83755997258396
23	20.34726981951108	20.287868403015764	37.386337674206075	21.978524103267077
24	16.897418323052317	21.69979437971213	24.94402558830249	36.45876170893306
25	22.80517343814268	23.35816461770486	35.555961793336685	18.280700150815775
26	39.865575419505284	22.632709981253715	18.023867221434777	19.477847377806228
27	21.555982436882545	37.449688986461766	18.29948774240761	22.69484083424808
28	29.09298606351382	31.16746630111949	20.849897189856065	18.889650445510622
29	32.019772071948374	29.502494393336082	20.30298869513479	18.174744839580757
30	28.308873954190094	21.291089471037353	30.96968865724866	19.43034791752389
31	31.963804213701387	29.67871669484941	20.360129792971073	17.99734929847813
32	31.118321831726153	30.3596727754673	20.871989397193914	17.65001599561263
33	30.705887735029048	28.528294981472165	20.53158881925065	20.234228464248137
34	21.587214360947016	23.890644319274625	36.72207720840775	17.80006411137061
35	20.194947830862166	22.77137104155226	39.54786747208494	17.48581365550064
36	28.192583786749577	22.522975629829457	30.04435096703397	19.240089616386996
37	27.07265739385066	21.50896778916545	31.91343338213763	19.504941434846266
38	30.422638399194103	21.429552635193918	29.493108658821377	18.654700306790602
39	28.69175135036162	22.17339558729287	21.614940950288382	27.519912112057128
40	22.894955270681546	18.126902148851794	29.52135017983953	29.456792400627137
41	20.784803105934554	16.038084673691994	27.43575522277685	35.7413569975966
42	21.626575028636886	29.754868270332185	22.611683848797252	26.006872852233677
43	18.006784633721463	26.661776840561107	30.058677913266706	25.272760612450718
44	19.812706573632024	29.035071612192436	28.135328681601173	23.016893132574367
45	26.25337667689208	21.702303008104025	31.042534682477907	21.001785632525984
46	17.69615261154675	31.26060439308479	22.59824826890448	28.444994726463978
47	16.815134539443473	27.105335659840208	22.499770410506017	33.579759390210306
48	27.42363270532285	24.437534296689225	22.873605267971463	25.265227730016466
49	18.390067347780274	28.528886241810604	21.881156365968753	31.19989004444037
50	20.440496359723433	31.36132606804341	22.130134163652183	26.068043408580976
51	19.534009081319084	30.839792689079488	24.15722606980691	25.46897215979452
52	20.147442648472914	37.97792939237144	24.117404643069737	17.757223316085902
53	20.10725089375745	34.68237235310294	25.12604271702264	20.084334036116967
54	19.976169744741306	25.273818798405205	29.897804866871365	24.852206589982124
55	18.20098061678046	25.106538972643545	30.78403519222838	25.908445218347616
56	20.079714128642113	23.346160894264248	33.44328385559832	23.130841121495326
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	0.5
7	0.0
8	0.5
9	1.0
10	7.5
11	14.0
12	27.0
13	46.5
14	53.0
15	53.5
16	54.0
17	50.0
18	46.0
19	65.5
20	85.0
21	82.0
22	84.5
23	90.0
24	94.0
25	98.0
26	120.0
27	142.0
28	168.0
29	187.0
30	180.0
31	192.0
32	204.0
33	238.0
34	272.0
35	297.5
36	323.0
37	344.0
38	398.0
39	431.0
40	462.5
41	494.0
42	592.5
43	691.0
44	911.5
45	1132.0
46	1687.0
47	2276.5
48	2311.0
49	1942.0
50	1573.0
51	1421.0
52	1269.0
53	1329.5
54	1414.5
55	1439.0
56	1304.0
57	1169.0
58	1151.5
59	1134.0
60	1079.5
61	1025.0
62	956.0
63	752.0
64	617.0
65	578.0
66	539.0
67	470.5
68	402.0
69	344.0
70	286.0
71	242.0
72	180.0
73	162.0
74	112.0
75	62.0
76	49.5
77	37.0
78	43.0
79	65.5
80	82.0
81	49.5
82	17.0
83	11.5
84	6.0
85	3.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.004569339730408956
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.516335389536212
16	0.05026273703449851
17	0.018277358921635824
18	0.0
19	0.0411240575736806
20	0.1370801919122687
21	0.0
22	0.0
23	0.0
24	0.0
25	0.018277358921635824
26	0.06397075622572539
27	0.09595613433858807
28	0.0
29	0.1644962302947224
30	0.054832076764907464
31	0.018277358921635824
32	0.018277358921635824
33	0.11880283299063284
34	0.21932830705962986
35	0.15078821110349555
36	0.06397075622572539
37	0.1325108521818597
38	0.21018962759881196
39	0.17820424948594926
40	0.9092986063513822
41	1.13776559287183
42	0.2741603838245374
43	0.32442312085903585
44	0.4615033127713045
45	0.20105094813799404
46	0.3564084989718985
47	0.4889193511537583
48	0.07767877541695226
49	0.2650217043637194
50	0.21018962759881196
51	0.37468585789353437
52	0.21018962759881196
53	0.30614576193740006
54	0.2924377427461732
55	0.28329906328535526
56	0.26045236463331045
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	21885.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.0827050491204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.34188614075124	66.18688599497372
2	6.45778079319065	9.568197395476354
3	1.8071917596990068	4.016449623029472
4	0.8635045950780238	2.558830249029015
5	0.4070807376796398	1.5078821110349554
6	0.3639055079257386	1.6175462645647705
7	0.14802935915623266	0.7676490747087046
8	0.06167889964843027	0.3655471784327165
9	0.0678467896132733	0.45236463331048665
>10	0.4317522975390119	5.9447109892620515
>50	0.018503669894529083	0.9687000228466986
>100	0.024671559859372107	3.660041124057574
>500	0.006167889964843027	2.385195339273475
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGAGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT	522	2.385195339273475	No Hit
CGAGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	376	1.7180717386337676	No Hit
CGAGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	189	0.8636052090472925	No Hit
CGAGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC	123	0.5620287868403016	No Hit
CGAGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT	113	0.516335389536212	No Hit
CGAGTAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT	87	0.3975325565455792	No Hit
CGAGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA	67	0.30614576193740006	No Hit
CGAGTAGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC	58	0.2650217043637194	No Hit
CGAGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC	50	0.2284669865204478	No Hit
CGAGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG	48	0.21932830705962986	No Hit
CGAGTAGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT	43	0.1964816084075851	No Hit
CGAGTAGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT	42	0.19191226867717615	No Hit
CGAGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT	40	0.18277358921635825	No Hit
CGAGTAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA	34	0.1553575508339045	No Hit
CGAGTAGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC	32	0.1462188713730866	No Hit
CGAGTAGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC	31	0.14164953164267763	No Hit
CGAGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG	28	0.12794151245145077	No Hit
CGAGTAGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT	28	0.12794151245145077	No Hit
CGAGTAGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG	27	0.12337217272104181	No Hit
CGAGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC	26	0.11880283299063284	No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	25	0.1142334932602239	No Hit
CGAGTAGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC	24	0.10966415352981493	No Hit
CGAGTAGGGATTGACAGTCTTGCATTTTGTTTTGTCTCGTGATGGTGGTGGTGGTA	24	0.10966415352981493	No Hit
CGAGTAGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG	23	0.10509481379940598	No Hit
CGAGTAGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC	22	0.10052547406899702	No Hit
CGAGTAGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG	22	0.10052547406899702	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.009138679460817912	0.0	0.0	0.0	0.0
7	0.009138679460817912	0.0	0.0	0.0	0.0
8	0.009138679460817912	0.0	0.0	0.0	0.0
9	0.009138679460817912	0.0	0.0	0.0	0.0
10	0.013708019191226868	0.0	0.0	0.0	0.0
11	0.013708019191226868	0.0	0.0	0.0	0.0
12	0.013708019191226868	0.0	0.0	0.0	0.0
13	0.013708019191226868	0.0	0.0	0.0	0.0
14	0.027416038382453736	0.0	0.0	0.0	0.0
15	0.027416038382453736	0.0	0.0	0.0	0.0
16	0.027416038382453736	0.0	0.0	0.0	0.0
17	0.027416038382453736	0.0	0.0	0.0	0.0
18	0.027416038382453736	0.0	0.0	0.0	0.0
19	0.027416038382453736	0.0	0.0	0.0	0.0
20	0.03198537811286269	0.0	0.0	0.0	0.0
21	0.03655471784327165	0.0	0.0	0.0	0.0
22	0.03655471784327165	0.0	0.0	0.0	0.0
23	0.03655471784327165	0.0	0.0	0.0	0.0
24	0.0411240575736806	0.0	0.0	0.0	0.0
25	0.0411240575736806	0.0	0.0	0.0	0.0
26	0.045693397304089556	0.0	0.0	0.0	0.0
27	0.059401416495316425	0.0	0.0	0.0	0.0
28	0.059401416495316425	0.0	0.0	0.0	0.0
29	0.06397075622572539	0.0	0.0	0.0	0.0
30	0.06397075622572539	0.0	0.0	0.0	0.0
31	0.06854009595613433	0.0	0.0	0.0	0.0
32	0.06854009595613433	0.0	0.0	0.0	0.0
33	0.0731094356865433	0.0	0.0	0.0	0.0
34	0.0731094356865433	0.0	0.0	0.0	0.0
35	0.07767877541695226	0.0	0.0	0.0	0.0
36	0.07767877541695226	0.0	0.0	0.0	0.0
37	0.07767877541695226	0.0	0.0	0.0	0.0
38	0.07767877541695226	0.0	0.0	0.0	0.0
39	0.07767877541695226	0.0	0.0	0.0	0.0
40	0.07767877541695226	0.0	0.0	0.0	0.0
41	0.07767877541695226	0.0	0.0	0.0	0.0
42	0.0822481151473612	0.0	0.0	0.0	0.0
43	0.0822481151473612	0.0	0.0	0.0	0.0
44	0.10052547406899703	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTGAA	25	1.95932E-5	50.258064	41
TGTCCAA	20	4.0362205E-4	50.142532	38
GTCCAAT	20	4.0362205E-4	50.142532	39
TCCAATC	20	4.0362205E-4	50.142532	40
TTTGTCC	20	4.0362205E-4	50.142532	36
TTGCTCA	20	4.0362205E-4	50.142532	37
ATCCCCC	15	0.008227841	50.14253	10
GGCGCAT	25	1.9863335E-5	50.14253	37
TTGGGCC	15	0.008227841	50.14253	34
GCGCATT	25	1.9863335E-5	50.14253	38
GACGAAA	15	0.008227841	50.14253	36
GTGACGA	15	0.008227841	50.14253	34
TGGGCGC	30	9.813521E-7	50.14253	35
GGGCGCA	30	9.813521E-7	50.14253	36
CGCATTG	25	1.9863335E-5	50.14253	39
GCCGATT	15	0.008227841	50.14253	38
GCATTGA	25	1.9863335E-5	50.14253	40
CCGATTG	15	0.008227841	50.14253	39
TGACGAA	15	0.008227841	50.14253	35
CGATTGA	15	0.008227841	50.14253	40
>>END_MODULE