FastQCFastQC Report
Fri 17 Jun 2016
SRR1526129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4504
Sequences flagged as poor quality0
Sequence length40
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC1162.575488454706927No Hit
CGAGTAGGGATGAGATGGACTTAAATGAATTGACGGGTAC972.153641207815275No Hit
CGAGTAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC781.7317939609236235No Hit
CGAGTAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC691.5319715808170513No Hit
CGAGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC611.3543516873889876No Hit
CGAGTAGGGATGAGATGGACTCAAATGAATTGACGGGTAC561.2433392539964476No Hit
CGAGTAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC531.1767317939609236No Hit
CGAGTAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC350.7770870337477798No Hit
CGAGTAGGGATGAGATGGACTTACAGGAATTGACGGGTAC190.4218472468916519No Hit
CGAGTAGGGATGAGATGGACTTACATGAATTGACGGGTAC130.2886323268206039No Hit
CGAGTAGGGATGAGATGGACTTAACGGAATTGACGGGTAC120.2664298401420959No Hit
CGAGTAGGGATGAGATGGCCTCCAAGGAATTGACGGGTAC110.24422735346358795No Hit
CGAGTAGGGATGAGATGGACTTAAAGGAATTGACCGGTAC90.19982238010657194No Hit
CGAGTAGGGATGAGATGGACTCAAAGGAATTGACCGGTAC90.19982238010657194No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGATGAGATGGACT80.17761989342806395No Hit
CGAGTAGGGATGAGATGGACTTAAATGAATTGACCGGTAC80.17761989342806395No Hit
CGAGTAGGGATGAGCTGGACTTAAATGAATTGACGGGTAC70.15541740674955595No Hit
CGAGTAGGGATGAGATGGACTCACATGAATTGACGGGTAC70.15541740674955595No Hit
CGAGTAGGGATGAGATGGACTCCAAGGAATTGACGGGTAC60.13321492007104796No Hit
CGAGTAGGGATGAGATGGACTCACAGGAATTGACGGGTAC60.13321492007104796No Hit
CGAGTAGGGATGAGATGGACTCCAATGAATTGACGGGTAC60.13321492007104796No Hit
CGAGTAGGGGAGTGCAGTGCTCGAGTAGGGATGAGATGGA60.13321492007104796No Hit
CGAGTAGGGATGAGATGGACTTAACTGAATTGACGGGTAC60.13321492007104796No Hit
CGAGTAGGGATGAGATGGACTCAAATGAATTGACGGGTCC60.13321492007104796No Hit
CGAGTAGGGATGAGCTGGACTCAAATGAATTGACGGGTAC60.13321492007104796No Hit
CGAGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTCC50.11101243339253995No Hit
CGAGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT50.11101243339253995No Hit
CGAGTAGGGATGAGATGGCCTTAAAGGAATTGCCGGGTAC50.11101243339253995No Hit
CGAGTAGGGGTCTTTATGAATTGAGGGTGCAGCTTGCTGC50.11101243339253995No Hit
CGAGTAGGGATGAGATGGACTTAAAGGCATTGACGGGTAC50.11101243339253995No Hit
CGAGTAGGGATGAGATGGACTCAACGGAATTGACGGGTAC50.11101243339253995No Hit
CGAGTAGGGCTGAGATGGACTCAAAGGAATTGACGGGTAC50.11101243339253995No Hit
CGAGTAGGGATGAGATGGACTTACAGGAATTGACCGGTAC50.11101243339253995No Hit
CGAGTAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGA50.11101243339253995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCAAA357.0839815E-734.53409219
ACTTACA200.002315131734.53409219
CCTCAAA357.0839815E-734.53409219
GACTCAA357.0839815E-734.53409218
GACTTAC200.002315131734.53409218
ACTTAAA301.0574689E-534.5340919
GACTTAA301.0574689E-534.5340918
GATGGAC850.034.1460714
ATGGACT850.034.1460715
AAAGGAA650.034.1460723
TGGACTT502.3101165E-1034.1460716
CTTAAAG251.6799787E-434.1460720
AAGGAAT650.034.1460724
GGAATTG650.034.1460726
CAAATGA301.1457623E-534.1460722
TAAAGGA251.6799787E-434.1460722
TTAAAGG251.6799787E-434.1460721
CTCAAAT301.1457623E-534.1460720
AGGAATT700.034.14606525
TCAAAGG405.230322E-834.14606521
Produced by FastQC (version 0.11.5)