##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526129_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4504
Sequences flagged as poor quality	0
Sequence length	40
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	21.670293072824155	30.0	4.0	32.0	4.0	33.0
2	12.666296625222024	4.0	4.0	20.0	4.0	29.0
3	8.939387211367674	4.0	4.0	12.0	4.0	19.0
4	12.77797513321492	4.0	4.0	22.0	4.0	30.0
5	9.758436944937833	4.0	4.0	12.0	4.0	24.0
6	12.152753108348135	4.0	4.0	20.0	4.0	29.0
7	14.093028419182948	4.0	4.0	24.0	4.0	30.0
8	13.08525754884547	4.0	4.0	22.0	4.0	29.0
9	13.554840142095914	4.0	4.0	23.0	4.0	30.0
10	10.060612788632326	4.0	4.0	13.0	4.0	25.0
11	15.824822380106571	4.0	4.0	30.0	4.0	34.0
12	16.128774422735347	4.0	4.0	31.0	4.0	35.0
13	14.867451154529308	4.0	4.0	28.0	4.0	33.0
14	15.909191829484902	4.0	4.0	30.0	4.0	34.0
15	14.095248667850798	4.0	4.0	27.0	4.0	33.0
16	13.575266429840141	4.0	4.0	26.0	4.0	32.0
17	13.445603907637656	4.0	4.0	26.0	4.0	32.0
18	13.082815275310836	4.0	4.0	25.0	4.0	32.0
19	11.744893428063943	4.0	4.0	21.0	4.0	31.0
20	13.191607460035524	4.0	4.0	27.0	4.0	33.0
21	11.034857904085257	4.0	4.0	19.0	4.0	31.0
22	10.9449378330373	4.0	4.0	18.0	4.0	31.0
23	10.029751332149202	4.0	4.0	14.0	4.0	30.0
24	9.828818827708703	4.0	4.0	12.0	4.0	30.0
25	9.663188277087034	4.0	4.0	11.0	4.0	30.0
26	9.115896980461812	4.0	4.0	4.0	4.0	30.0
27	9.228685612788633	4.0	4.0	4.0	4.0	31.0
28	8.480905861456483	4.0	4.0	4.0	4.0	28.0
29	8.436056838365896	4.0	4.0	4.0	4.0	28.0
30	7.949822380106572	4.0	4.0	4.0	4.0	26.0
31	7.697380106571936	4.0	4.0	4.0	4.0	26.0
32	7.382104795737122	4.0	4.0	4.0	4.0	23.0
33	7.448934280639431	4.0	4.0	4.0	4.0	24.0
34	7.676953818827709	4.0	4.0	4.0	4.0	28.0
35	7.0444049733570155	4.0	4.0	4.0	4.0	23.0
36	7.279307282415631	4.0	4.0	4.0	4.0	25.0
37	7.1698490230905865	4.0	4.0	4.0	4.0	23.0
38	7.1696269982238015	4.0	4.0	4.0	4.0	24.0
39	7.128774422735346	4.0	4.0	4.0	4.0	22.0
40	7.149866785079929	4.0	4.0	4.0	4.0	26.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	9.28268362550541
1	2	11.786051105110513
1	3	1.5654797622619405
1	4	10.426706599231354
1	5	14.163347763347764
1	6	14.917968225393968
1	7	13.32786528652865
1	8	12.723779163630653
1	9	14.579191847756206
1	10	11.232450030717354
1	11	6.943927964224994
1	12	5.576843041446999
1	13	1.147672624405292
1	14	9.406952123783809
1	15	7.555508050805077
1	16	10.902029131484579
1	17	10.453440344034403
1	18	6.675113939965426
1	19	2.8336008600860083
1	20	6.991621662166219
1	21	14.369654465446544
1	22	15.055917377452031
1	23	11.025300030003
1	24	16.413871387138713
1	25	14.441224836769392
1	26	5.732565399397082
1	27	8.058530138728157
1	28	10.039082479676537
1	29	9.926214407155
1	30	7.896269984141268
1	31	14.029392582115353
1	32	12.273008015087223
1	33	13.459747760490334
1	34	14.203162816281628
1	35	13.773526638378124
1	36	10.65096652522395
1	37	12.072721200691497
1	38	15.061122898004086
1	39	16.543289328932893
1	40	16.049249567813924
5	1	12.53268362550541
5	2	-3.963948894889487
5	3	21.19047976226194
5	4	17.551706599231352
5	5	-5.211652236652236
5	6	-7.5820317746060315
5	7	-9.54713471347135
5	8	-8.401220836369347
5	9	-9.045808152243794
5	10	-5.517549969282646
5	11	-10.931072035775006
5	12	-11.673156958553001
5	13	-10.477327375594708
5	14	-11.218047876216191
5	15	-9.569491949194923
5	16	-9.222970868515421
5	17	-8.796559655965597
5	18	-8.574886060034574
5	19	-6.916399139913992
5	20	-8.633378337833781
5	21	-6.005345534553456
5	22	-6.069082622547969
5	23	-5.099699969996999
5	24	-4.8361286128612875
5	25	-4.683775163230608
5	26	-4.892434600602918
5	27	-4.566469861271843
5	28	-4.210917520323463
5	29	-4.073785592845001
5	30	-3.8537300158587318
5	31	-2.970607417884647
5	32	-2.726991984912777
5	33	-2.790252239509666
5	34	-3.046837183718372
5	35	-2.476473361621876
5	36	-2.84903347477605
5	37	-2.927278799308503
5	38	-2.9388771019959137
5	39	-2.831710671067106
5	40	-2.9507504321860765
6	1	2.4076836255054097
6	2	-4.338948894889487
6	3	-1.8095202377380595
6	4	-6.698293400768646
6	5	-3.0866522366522364
6	6	-2.4570317746060315
6	7	-2.5471347134713493
6	8	-5.5262208363693475
6	9	-2.5458081522437936
6	10	-2.017549969282646
6	11	-2.6810720357750064
6	12	-4.923156958553001
6	13	-0.9773273755947081
6	14	-2.9680478762161915
6	15	-2.944491949194923
6	16	-3.5979708685154215
6	17	-2.7965596559655967
6	18	-2.574886060034574
6	19	1.4586008600860083
6	20	-1.383378337833781
6	21	-1.2553455345534559
6	22	0.0559173774520314
6	23	-1.7246999699969994
6	24	0.16387138713871252
6	25	-1.0587751632306084
6	26	0.9825653993970818
6	27	-0.19146986127184284
6	28	-0.5859175203234628
6	29	0.6762144071549994
6	30	1.2712699841412682
6	31	-0.0956074178846471
6	32	0.8980080150872229
6	33	-0.790252239509666
6	34	0.4531628162816279
6	35	1.148526638378124
6	36	-0.34903347477604996
6	37	0.5727212006914968
6	38	-1.0638771019959137
6	39	-1.206710671067106
6	40	-0.7007504321860765
7	1	-2.103680010858227
7	2	-4.691221622162215
7	3	-3.7186111468289686
7	4	-6.81192976440501
7	5	-3.484379509379509
7	6	-3.854759047333304
7	7	-4.819861986198623
7	8	-3.673948109096621
7	9	-4.954899061334702
7	10	-3.6993681511008276
7	11	-1.4765265812295514
7	12	-0.7640660494620928
7	13	-2.1136910119583447
7	14	-1.6725933307616465
7	15	-1.4785828582858311
7	16	-1.6775163230608765
7	17	-2.4329232923292334
7	18	-3.574886060034574
7	19	-3.0073082308230825
7	20	-1.178832883288326
7	21	-2.45989098909891
7	22	-1.1599917134570603
7	23	-3.4633363336333627
7	24	-2.3815831583158333
7	25	-2.2292297086851542
7	26	-3.437889146057464
7	27	-2.6573789521809337
7	28	-2.4836447930507353
7	29	-2.1646946837540915
7	30	-1.4900936522223684
7	31	-2.970607417884647
7	32	-2.726991984912777
7	33	-2.790252239509666
7	34	-3.046837183718372
7	35	-2.476473361621876
7	36	-2.84903347477605
7	37	-2.927278799308503
7	38	-2.9388771019959137
7	39	-2.831710671067106
7	40	-2.9507504321860765
8	1	-16.46731637449459
8	2	-7.963948894889487
8	3	-4.8095202377380595
8	4	-8.448293400768646
8	5	-5.211652236652236
8	6	-7.5820317746060315
8	7	-9.54713471347135
8	8	-8.401220836369347
8	9	-9.045808152243794
8	10	-5.517549969282646
8	11	-10.931072035775006
8	12	-11.673156958553001
8	13	-10.477327375594708
8	14	-11.218047876216191
8	15	-9.569491949194923
8	16	-9.222970868515421
8	17	-8.796559655965597
8	18	-8.574886060034574
8	19	-6.916399139913992
8	20	-8.633378337833781
8	21	-6.005345534553456
8	22	-6.069082622547969
8	23	-5.099699969996999
8	24	-4.8361286128612875
8	25	-4.683775163230608
8	26	-4.892434600602918
8	27	-4.566469861271843
8	28	-4.210917520323463
8	29	-4.073785592845001
8	30	-3.8537300158587318
8	31	-2.970607417884647
8	32	-2.726991984912777
8	33	-2.790252239509666
8	34	-3.046837183718372
8	35	-2.476473361621876
8	36	-2.84903347477605
8	37	-2.927278799308503
8	38	-2.9388771019959137
8	39	-2.831710671067106
8	40	-2.9507504321860765
9	1	-0.6895385967168117
9	2	-4.741726672667265
9	3	-3.587298015515837
9	4	-4.892737845213091
9	5	-3.5449855699855695
9	6	-5.470920663494921
9	7	-4.65824582458246
9	8	-3.4012208363693475
9	9	-4.045808152243794
9	10	-0.7397721915048674
9	11	-4.5977387024416725
9	12	-8.117601402997446
9	13	-4.921771820039153
9	14	-6.662492320660636
9	15	-2.7917141714171443
9	16	-3.667415312959866
9	17	-3.685448544854486
9	18	-3.574886060034574
9	19	-2.471954695469547
9	20	-3.633378337833781
9	21	-0.4497899789979005
9	22	0.04202848856314212
9	23	1.78918891889189
9	24	1.386093609360934
9	25	2.09400261454717
9	26	-1.4479901561584736
9	27	-1.1220254168273982
9	28	-1.099806409212352
9	29	-1.629341148400556
9	30	-0.4092855714142871
9	31	-0.30394075121798014
9	32	-0.06032531824611009
9	33	0.20974776049033395
9	34	-0.713503850385039
9	35	0.30130441615590176
9	36	0.5954109696683947
9	37	0.5171656451359414
9	38	0.505567342448531
9	39	0.6127337733773386
9	40	0.4936940122583682
11	1	0.421572514394299
11	2	-4.29728222822282
11	3	-2.587298015515837
11	4	-5.781626734101979
11	5	-2.989430014430014
11	6	-3.137587330161587
11	7	-0.8804680468046833
11	8	-1.5123319474804582
11	9	-1.601363707799349
11	10	0.48245003071735404
11	11	4.402261297558326
11	12	4.326843041446999
11	13	3.522672624405292
11	14	4.00417434600603
11	15	5.097174717471745
11	16	5.9992513537068
11	17	6.314551455145514
11	18	7.091780606632094
11	19	3.4169341934193422
11	20	6.811066106610662
11	21	4.66132113211321
11	22	5.70869515522981
11	23	8.011411141114111
11	24	2.941649164916491
11	25	5.205113725658281
11	26	6.663120954952637
11	27	1.6557523609503786
11	28	4.011304701898759
11	29	-3.1848967039561114
11	30	-3.075952238080954
11	31	-2.30394075121798
11	32	-1.2825475404683324
11	33	-2.123585572842999
11	34	-2.4912816281628167
11	35	-1.9209178060663206
11	36	0.4842998585572831
11	37	0.07272120069149679
11	38	0.06112289800408632
11	39	0.16828932893289394
11	40	0.049249567813923534
12	1	-5.610173517351733
12	2	-5.535377466318058
12	3	-3.6666630948809162
12	4	-6.448293400768646
12	5	-3.6402236652236653
12	6	-4.724888917463175
12	7	-6.404277570614206
12	8	-5.829792264940776
12	9	-5.760093866529508
12	10	-3.946121397854075
12	11	-6.64535775006072
12	12	-4.9588712442672875
12	13	-6.191613089880422
12	14	-4.075190733359049
12	15	-1.8552062349092093
12	16	-1.2229708685154215
12	17	-1.0822739416798832
12	18	-2.2891717743202875
12	19	0.22645800294315066
12	20	-0.9190926235480674
12	21	0.8517973225894018
12	22	1.788060234594889
12	23	-1.6711285414255705
12	24	-3.121842898575573
12	25	-0.969489448944894
12	26	-4.892434600602918
12	27	-4.566469861271843
12	28	-4.210917520323463
12	29	-4.073785592845001
12	30	-3.8537300158587318
12	31	-2.970607417884647
12	32	-2.726991984912777
12	33	-2.790252239509666
12	34	-3.046837183718372
12	35	-2.476473361621876
12	36	-2.84903347477605
12	37	-2.927278799308503
12	38	-2.9388771019959137
12	39	-2.831710671067106
12	40	-2.9507504321860765
13	1	15.232683625505413
13	2	21.33605110511051
13	3	17.19047976226194
13	4	19.85170659923135
13	5	22.588347763347763
13	6	22.217968225393967
13	7	17.65286528652865
13	8	20.098779163630653
13	9	20.15419184775621
13	10	21.582450030717354
13	11	17.36892796422499
13	12	12.326843041446999
13	13	14.92267262440529
13	14	16.18195212378381
13	15	16.230508050805078
13	16	15.87702913148458
13	17	18.503440344034402
13	18	20.52511393996543
13	19	13.783600860086008
13	20	19.366621662166217
13	21	18.994654465446544
13	22	22.73091737745203
13	23	23.600300030003005
13	24	23.363871387138715
13	25	24.016224836769396
13	26	17.00756539939708
13	27	14.933530138728157
13	28	8.289082479676537
13	29	12.326214407154998
13	30	8.546269984141267
13	31	11.329392582115354
13	32	11.073008015087224
13	33	10.909747760490333
13	34	8.753162816281629
13	35	6.423526638378124
13	36	9.15096652522395
13	37	6.772721200691496
13	38	8.461122898004087
13	39	6.468289328932895
13	40	5.749249567813923
14	1	12.53268362550541
14	2	13.036051105110513
14	3	6.1904797622619405
14	4	17.551706599231352
14	5	17.788347763347765
14	6	18.41796822539397
14	7	19.45286528652865
14	8	22.598779163630653
14	9	21.954191847756206
14	10	23.482450030717352
14	11	12.068927964224994
14	12	20.326843041447
14	13	12.522672624405292
14	14	11.781952123783809
14	15	9.430508050805077
14	16	17.77702913148458
14	17	20.203440344034405
14	18	20.425113939965428
14	19	-6.916399139913992
14	20	-8.633378337833781
14	21	-6.005345534553456
14	22	-6.069082622547969
14	23	-5.099699969996999
14	24	-4.8361286128612875
14	25	-4.683775163230608
14	26	-4.892434600602918
14	27	-4.566469861271843
14	28	-4.210917520323463
14	29	-4.073785592845001
14	30	-3.8537300158587318
14	31	-2.970607417884647
14	32	-2.726991984912777
14	33	-2.790252239509666
14	34	-3.046837183718372
14	35	-2.476473361621876
14	36	-2.84903347477605
14	37	-2.927278799308503
14	38	-2.9388771019959137
14	39	-2.831710671067106
14	40	-2.9507504321860765
15	1	4.088239181060967
15	2	-3.6306155615561533
15	3	-2.365075793293615
15	4	-5.559404511879757
15	5	-2.2116522366522364
15	6	-3.026476219050476
15	7	-1.8804680468046833
15	8	-0.7345541697026814
15	9	-2.7124748189104597
15	10	-1.4064388581715352
15	11	4.624483519780551
15	12	2.4379541525581097
15	13	3.3004504021830705
15	14	4.559729901561587
15	15	3.3193969396939664
15	16	2.1103624648179125
15	17	2.314551455145514
15	18	-2.4637749489234633
15	19	0.9724897489748976
15	20	0.8110661066106637
15	21	4.327987798779878
15	22	-3.8468604003257463
15	23	-1.4330333033303324
15	24	-0.2805730573057321
15	25	-0.7948862743417191
15	26	1.3297876216193032
15	27	-2.455358750160732
15	28	-1.6553619647679074
15	29	-1.5182300372894453
15	30	-1.6315077936365094
15	31	-0.7483851956624248
15	32	-0.2825475404683324
15	33	0.6541922049347786
15	34	0.06427392739273863
15	35	0.6346377494892348
15	36	0.26207763633506076
15	37	-0.48283435486405857
15	38	0.17223400911519704
15	39	0.27940044004400466
15	40	-0.5063059877416318
16	1	-6.06731637449459
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107	28	5.646225336819395
107	29	3.783357264297857
107	30	2.8605556984269818
107	31	-0.9706074178846471
107	32	-2.2984205563413482
107	33	0.06689061763319071
107	34	-1.1896943265755153
107	35	0.2378123526638385
107	36	0.2938236680810933
107	37	1.3584354864057833
107	38	1.0611228980040863
107	39	1.168289328932894
107	40	1.0492495678139235
108	1	-10.71731637449459
108	2	-4.213948894889487
108	3	-2.5595202377380595
108	4	-4.948293400768646
108	5	-3.7116522366522364
108	6	-2.8320317746060315
108	7	-4.547134713471349
108	8	-3.4012208363693475
108	9	-4.045808152243794
108	10	-1.017549969282646
108	11	-2.4310720357750064
108	12	-3.173156958553001
108	13	-2.727327375594708
108	14	-4.4680478762161915
108	15	-3.319491949194923
108	16	-4.9729708685154215
108	17	-5.296559655965597
108	18	-6.074886060034574
108	19	-0.4163991399139917
108	20	-1.883378337833781
108	21	-6.005345534553456
108	22	-6.069082622547969
108	23	-5.099699969996999
108	24	-4.8361286128612875
108	25	-4.683775163230608
108	26	-4.892434600602918
108	27	-4.566469861271843
108	28	-4.210917520323463
108	29	-4.073785592845001
108	30	-3.8537300158587318
108	31	-2.970607417884647
108	32	-2.726991984912777
108	33	-2.790252239509666
108	34	-3.046837183718372
108	35	-2.476473361621876
108	36	-2.84903347477605
108	37	-2.927278799308503
108	38	-2.9388771019959137
108	39	-2.831710671067106
108	40	-2.9507504321860765
109	1	-1.6101735173517326
109	2	-4.535377466318058
109	3	-2.6666630948809162
109	4	-5.59115054362579
109	5	-4.068795093795093
109	6	-4.5820317746060315
109	7	-4.1185632848999205
109	8	-4.115506550655061
109	9	-4.760093866529507
109	10	-3.2318356835683604
109	11	-3.5025006072035776
109	12	-3.8160141014101434
109	13	-1.3344702327375657
109	14	-3.2180478762161915
109	15	-5.569491949194923
109	16	-5.794399439943993
109	17	-5.225131084537026
109	18	-4.574886060034574
109	19	-6.916399139913992
109	20	-8.633378337833781
109	21	-6.005345534553456
109	22	-6.069082622547969
109	23	-5.099699969996999
109	24	-4.8361286128612875
109	25	-4.683775163230608
109	26	-4.892434600602918
109	27	-4.566469861271843
109	28	-4.210917520323463
109	29	-4.073785592845001
109	30	-3.8537300158587318
109	31	-2.970607417884647
109	32	-2.726991984912777
109	33	-2.790252239509666
109	34	-3.046837183718372
109	35	-2.476473361621876
109	36	-2.84903347477605
109	37	-2.927278799308503
109	38	-2.9388771019959137
109	39	-2.831710671067106
109	40	-2.9507504321860765
110	1	-4.46731637449459
110	2	-5.963948894889487
110	3	-4.5595202377380595
110	4	-5.448293400768646
110	5	-4.711652236652236
110	6	-5.8320317746060315
110	7	-5.547134713471349
110	8	-6.1512208363693475
110	9	-6.295808152243794
110	10	-2.017549969282646
110	11	-5.931072035775006
110	12	-5.923156958553001
110	13	-7.227327375594708
110	14	-5.7180478762161915
110	15	-6.819491949194923
110	16	-5.9729708685154215
110	17	-6.046559655965597
110	18	-4.074886060034574
110	19	-6.666399139913992
110	20	-6.383378337833781
110	21	-4.005345534553456
110	22	-0.3190826225479686
110	23	-4.099699969996999
110	24	-3.0861286128612875
110	25	-3.1837751632306084
110	26	-1.3924346006029182
110	27	-1.0664698612718428
110	28	-1.9609175203234628
110	29	-2.0737855928450006
110	30	-1.6037300158587318
110	31	2.529392582115353
110	32	2.273008015087223
110	33	4.959747760490334
110	34	2.453162816281628
110	35	4.523526638378124
110	36	4.90096652522395
110	37	4.822721200691497
110	38	4.811122898004086
110	39	4.918289328932894
110	40	4.7992495678139235
111	1	2.1993502921720776
111	2	-7.963948894889487
111	3	-4.8095202377380595
111	4	-8.448293400768646
111	5	-5.211652236652236
111	6	-7.5820317746060315
111	7	-9.54713471347135
111	8	-8.401220836369347
111	9	-9.045808152243794
111	10	-5.517549969282646
111	11	-10.931072035775006
111	12	-11.673156958553001
111	13	-10.477327375594708
111	14	-11.218047876216191
111	15	-9.569491949194923
111	16	-9.222970868515421
111	17	-8.796559655965597
111	18	-8.574886060034574
111	19	-6.916399139913992
111	20	-8.633378337833781
111	21	-6.005345534553456
111	22	-6.069082622547969
111	23	-5.099699969996999
111	24	-4.8361286128612875
111	25	-4.683775163230608
111	26	-4.892434600602918
111	27	-4.566469861271843
111	28	-4.210917520323463
111	29	-4.073785592845001
111	30	-3.8537300158587318
111	31	-2.970607417884647
111	32	-2.726991984912777
111	33	-2.790252239509666
111	34	-3.046837183718372
111	35	-2.476473361621876
111	36	-2.84903347477605
111	37	-2.927278799308503
111	38	-2.9388771019959137
111	39	-2.831710671067106
111	40	-2.9507504321860765
112	1	9.28268362550541
112	2	0.7860511051105128
112	3	-0.3095202377380595
112	4	-0.6982934007686463
112	5	1.5383477633477636
112	6	-0.8320317746060315
112	7	6.452865286528651
112	8	0.8487791636306525
112	9	4.704191847756206
112	10	1.732450030717354
112	11	9.068927964224994
112	12	8.826843041446999
112	13	10.522672624405292
112	14	9.531952123783809
112	15	11.180508050805077
112	16	8.777029131484579
112	17	11.453440344034403
112	18	11.425113939965426
112	19	9.333600860086008
112	20	5.366621662166219
112	21	-2.005345534553456
112	22	-1.5690826225479686
112	23	0.40030003000300063
112	24	1.9138713871387125
112	25	0.3162248367693916
112	26	0.35756539939708176
112	27	1.6835301387281572
112	28	0.5390824796765372
112	29	1.6762144071549994
112	30	3.3962699841412682
112	31	4.279392582115353
112	32	3.023008015087223
112	33	4.959747760490334
112	34	4.203162816281628
112	35	4.773526638378124
112	36	4.40096652522395
112	37	4.822721200691497
112	38	4.561122898004086
112	39	4.918289328932894
112	40	4.7992495678139235
113	1	-16.46731637449459
113	2	-7.963948894889487
113	3	-4.8095202377380595
113	4	-8.448293400768646
113	5	-5.211652236652236
113	6	-7.5820317746060315
113	7	-9.54713471347135
113	8	-8.401220836369347
113	9	-9.045808152243794
113	10	-5.517549969282646
113	11	-10.931072035775006
113	12	-11.673156958553001
113	13	-10.477327375594708
113	14	-11.218047876216191
113	15	-9.569491949194923
113	16	-9.222970868515421
113	17	-8.796559655965597
113	18	-8.574886060034574
113	19	-6.916399139913992
113	20	-8.633378337833781
113	21	-6.005345534553456
113	22	-6.069082622547969
113	23	-5.099699969996999
113	24	-4.8361286128612875
113	25	-4.683775163230608
113	26	-4.892434600602918
113	27	-4.566469861271843
113	28	-4.210917520323463
113	29	-4.073785592845001
113	30	-3.8537300158587318
113	31	-2.970607417884647
113	32	-2.726991984912777
113	33	-2.790252239509666
113	34	-3.046837183718372
113	35	-2.476473361621876
113	36	-2.84903347477605
113	37	-2.927278799308503
113	38	-2.9388771019959137
113	39	-2.831710671067106
113	40	-2.9507504321860765
114	1	8.53268362550541
114	2	6.036051105110513
114	3	-1.8095202377380595
114	4	3.5517065992313537
114	5	0.7883477633477636
114	6	4.4179682253939685
114	7	10.45286528652865
114	8	4.5987791636306525
114	9	13.954191847756206
114	10	-0.517549969282646
114	11	15.068927964224994
114	12	20.326843041447
114	13	13.522672624405292
114	14	20.78195212378381
114	15	8.430508050805077
114	16	19.77702913148458
114	17	20.203440344034405
114	18	13.425113939965426
114	19	12.083600860086008
114	20	19.366621662166217
114	21	15.994654465446544
114	22	20.930917377452033
114	23	-5.099699969996999
114	24	-4.8361286128612875
114	25	-4.683775163230608
114	26	-4.892434600602918
114	27	-4.566469861271843
114	28	-4.210917520323463
114	29	-4.073785592845001
114	30	-3.8537300158587318
114	31	-2.970607417884647
114	32	-2.726991984912777
114	33	-2.790252239509666
114	34	-3.046837183718372
114	35	-2.476473361621876
114	36	-2.84903347477605
114	37	-2.927278799308503
114	38	-2.9388771019959137
114	39	-2.831710671067106
114	40	-2.9507504321860765
117	1	-3.9673163744945903
117	2	-1.4639488948894872
117	3	2.6904797622619405
117	4	-4.448293400768646
117	5	-0.21165223665223643
117	6	-4.0820317746060315
117	7	-1.0471347134713493
117	8	-7.4012208363693475
117	9	1.4541918477562064
117	10	-2.017549969282646
117	11	-3.9310720357750064
117	12	0.8268430414469989
117	13	-1.977327375594708
117	14	-5.2180478762161915
117	15	2.930508050805077
117	16	3.2770291314845785
117	17	4.203440344034403
117	18	6.425113939965426
117	19	5.583600860086008
117	20	1.366621662166219
117	21	5.494654465446544
117	22	8.430917377452031
117	23	-5.099699969996999
117	24	-4.8361286128612875
117	25	-4.683775163230608
117	26	-4.892434600602918
117	27	-4.566469861271843
117	28	-4.210917520323463
117	29	-4.073785592845001
117	30	-3.8537300158587318
117	31	-2.970607417884647
117	32	-2.726991984912777
117	33	-2.790252239509666
117	34	-3.046837183718372
117	35	-2.476473361621876
117	36	-2.84903347477605
117	37	-2.927278799308503
117	38	-2.9388771019959137
117	39	-2.831710671067106
117	40	-2.9507504321860765
118	1	-7.800649707827924
118	2	-6.630615561556154
118	3	-1.8095202377380595
118	4	-8.114960067435312
118	5	-1.8783189033189034
118	6	-2.5820317746060315
118	7	-0.8804680468046833
118	8	-5.067887503036014
118	9	-1.3791414855771276
118	10	-3.184216635949313
118	11	-2.2644053691083403
118	12	-1.3398236252196671
118	13	-1.810660708928042
118	14	-3.8847145428828576
118	15	-5.569491949194923
118	16	-0.5563042018487554
118	17	-2.463226322632263
118	18	0.09178060663209209
118	19	1.7502675267526744
118	20	1.699954995499553
118	21	3.994654465446544
118	22	4.5975840441186975
118	23	4.900300030003001
118	24	3.4972047204720464
118	25	3.9828915034360577
118	26	5.774232066063748
118	27	5.766863472061491
118	28	2.122415813009871
118	29	4.926214407154999
118	30	5.479603317474602
118	31	-2.970607417884647
118	32	-2.726991984912777
118	33	-2.790252239509666
118	34	-3.046837183718372
118	35	-2.476473361621876
118	36	-2.84903347477605
118	37	-2.927278799308503
118	38	-2.9388771019959137
118	39	-2.831710671067106
118	40	-2.9507504321860765
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	2267.0
5	11.0
6	31.0
7	18.0
8	19.0
9	76.0
10	105.0
11	104.0
12	156.0
13	171.0
14	142.0
15	164.0
16	121.0
17	139.0
18	101.0
19	86.0
20	102.0
21	115.0
22	94.0
23	85.0
24	80.0
25	79.0
26	60.0
27	58.0
28	35.0
29	31.0
30	25.0
31	14.0
32	10.0
33	3.0
34	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.13333333333333	0.5555555555555556	0.17777777777777778	0.13333333333333333
8	98.37921847246892	0.5550621669626998	0.5328596802841918	0.5328596802841918
9	98.60093271152564	0.7994670219853431	0.22207417277370642	0.37752609371530094
10	30.135465245391963	55.118809682433934	7.128580946035975	7.61714412613813
11	19.51598579040853	19.316163410301954	50.15541740674956	11.012433392539965
12	57.850322007550524	11.814345991561181	14.146124805685098	16.1892071952032
13	17.095914742451154	47.735346358792185	16.363232682060392	18.80550621669627
14	47.86856127886323	19.560390763765543	17.428952042628772	15.142095914742452
15	18.050621669627	48.13499111900533	16.896092362344582	16.91829484902309
16	16.25222024866785	15.697158081705151	52.02042628774423	16.03019538188277
17	50.799289520426285	17.939609236234457	15.097690941385435	16.163410301953817
18	51.30994671403197	16.785079928952044	13.587921847246893	18.317051509769094
19	14.51828724353256	30.84299732381802	13.693131132917038	40.94558429973238
20	15.852575488454706	18.650088809946713	13.698934280639433	51.79840142095915
21	16.82948490230906	16.91829484902309	48.31261101243339	17.939609236234457
22	16.074600355239788	17.073712255772648	33.348134991119004	33.50355239786856
23	18.761101243339255	47.269094138543515	16.096802841918294	17.873001776198937
24	14.9011769931157	47.945813901843216	16.877637130801688	20.275371974239395
25	16.7815070015559	48.87752833963103	16.559235385641255	17.781729273171816
26	33.48254252461952	18.912264995523724	31.55774395702775	16.047448522829004
27	47.72373972906951	16.699977792582725	18.676437930268712	16.899844548079056
28	15.304309195912927	47.334517992003555	19.768991559306976	17.59218125277654
29	14.021811707099932	44.558201647006456	21.366570220342755	20.053416425550857
30	12.733748886910062	16.80765805877115	52.93855743544078	17.520035618878005
31	12.703365277468242	16.358368620459103	55.24849565411187	15.689770447960777
32	43.647267880941804	15.437583296312749	25.277654375832963	15.637494446912484
33	12.366785079928952	43.3392539964476	25.688277087033747	18.605683836589698
34	9.7246891651865	16.74067495559503	27.131438721136767	46.4031971580817
35	36.14564831261101	15.563943161634104	27.575488454706925	20.714920071047956
36	39.9866785079929	16.651865008880996	27.46447602131439	15.896980461811722
37	37.90268829149078	17.418351477449455	28.082648300377695	16.59631193068207
38	11.567495559502666	17.273534635879216	55.48401420959147	15.674955595026644
39	11.700710479573711	44.13854351687389	26.909413854351687	17.25133214920071
40	10.124333925399645	19.649200710479573	26.26554174067496	43.96092362344582
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.0
15	4.0
16	9.5
17	15.0
18	15.0
19	17.5
20	20.0
21	27.5
22	35.0
23	35.0
24	43.5
25	52.0
26	64.0
27	76.0
28	76.0
29	67.5
30	59.0
31	69.0
32	79.0
33	79.0
34	94.5
35	110.0
36	121.5
37	133.0
38	133.0
39	147.5
40	162.0
41	189.5
42	217.0
43	217.0
44	274.5
45	332.0
46	471.5
47	611.0
48	611.0
49	613.5
50	616.0
51	526.5
52	437.0
53	437.0
54	366.0
55	295.0
56	275.0
57	255.0
58	255.0
59	240.5
60	226.0
61	219.0
62	212.0
63	212.0
64	213.0
65	214.0
66	180.5
67	147.0
68	147.0
69	119.5
70	92.0
71	74.0
72	56.0
73	56.0
74	40.5
75	25.0
76	19.0
77	13.0
78	13.0
79	10.5
80	8.0
81	5.0
82	2.0
83	2.0
84	1.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.08880994671403197
8	0.0
9	0.022202486678507993
10	0.022202486678507993
11	0.0
12	0.022202486678507993
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.4440497335701598
20	0.0
21	0.0
22	0.0
23	0.0
24	0.022202486678507993
25	0.11101243339253995
26	0.7992895204262878
27	0.022202486678507993
28	0.04440497335701599
29	0.24422735346358795
30	0.2664298401420959
31	0.3774422735346359
32	0.04440497335701599
33	0.0
34	0.0
35	0.0
36	0.0
37	0.06660746003552398
38	0.0
39	0.0
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	4504.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.9076376554174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.45694991558807	74.53374777975134
2	3.2357906584130554	5.106571936056838
3	0.8441193021947102	1.9982238010657194
4	0.5064715813168261	1.5985790408525755
5	0.25323579065841306	0.9991119005328597
6	0.19696117051209902	0.9325044404973356
7	0.056274620146314014	0.3108348134991119
8	0.056274620146314014	0.3552397868561279
9	0.056274620146314014	0.3996447602131439
>10	0.14068655036578503	1.9982238010657194
>50	0.16882386043894204	9.191829484902309
>100	0.028137310073157007	2.575488454706927
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGAGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC	116	2.575488454706927	No Hit
CGAGTAGGGATGAGATGGACTTAAATGAATTGACGGGTAC	97	2.153641207815275	No Hit
CGAGTAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC	78	1.7317939609236235	No Hit
CGAGTAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC	69	1.5319715808170513	No Hit
CGAGTAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC	61	1.3543516873889876	No Hit
CGAGTAGGGATGAGATGGACTCAAATGAATTGACGGGTAC	56	1.2433392539964476	No Hit
CGAGTAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC	53	1.1767317939609236	No Hit
CGAGTAGGGATGAGATGGCCTCAAATGAATTGACGGGTAC	35	0.7770870337477798	No Hit
CGAGTAGGGATGAGATGGACTTACAGGAATTGACGGGTAC	19	0.4218472468916519	No Hit
CGAGTAGGGATGAGATGGACTTACATGAATTGACGGGTAC	13	0.2886323268206039	No Hit
CGAGTAGGGATGAGATGGACTTAACGGAATTGACGGGTAC	12	0.2664298401420959	No Hit
CGAGTAGGGATGAGATGGCCTCCAAGGAATTGACGGGTAC	11	0.24422735346358795	No Hit
CGAGTAGGGATGAGATGGACTTAAAGGAATTGACCGGTAC	9	0.19982238010657194	No Hit
CGAGTAGGGATGAGATGGACTCAAAGGAATTGACCGGTAC	9	0.19982238010657194	No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGATGAGATGGACT	8	0.17761989342806395	No Hit
CGAGTAGGGATGAGATGGACTTAAATGAATTGACCGGTAC	8	0.17761989342806395	No Hit
CGAGTAGGGATGAGCTGGACTTAAATGAATTGACGGGTAC	7	0.15541740674955595	No Hit
CGAGTAGGGATGAGATGGACTCACATGAATTGACGGGTAC	7	0.15541740674955595	No Hit
CGAGTAGGGATGAGATGGACTCCAAGGAATTGACGGGTAC	6	0.13321492007104796	No Hit
CGAGTAGGGATGAGATGGACTCACAGGAATTGACGGGTAC	6	0.13321492007104796	No Hit
CGAGTAGGGATGAGATGGACTCCAATGAATTGACGGGTAC	6	0.13321492007104796	No Hit
CGAGTAGGGGAGTGCAGTGCTCGAGTAGGGATGAGATGGA	6	0.13321492007104796	No Hit
CGAGTAGGGATGAGATGGACTTAACTGAATTGACGGGTAC	6	0.13321492007104796	No Hit
CGAGTAGGGATGAGATGGACTCAAATGAATTGACGGGTCC	6	0.13321492007104796	No Hit
CGAGTAGGGATGAGCTGGACTCAAATGAATTGACGGGTAC	6	0.13321492007104796	No Hit
CGAGTAGGGATGAGATGGACTTAAAGGAATTGACGGGTCC	5	0.11101243339253995	No Hit
CGAGTAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.11101243339253995	No Hit
CGAGTAGGGATGAGATGGCCTTAAAGGAATTGCCGGGTAC	5	0.11101243339253995	No Hit
CGAGTAGGGGTCTTTATGAATTGAGGGTGCAGCTTGCTGC	5	0.11101243339253995	No Hit
CGAGTAGGGATGAGATGGACTTAAAGGCATTGACGGGTAC	5	0.11101243339253995	No Hit
CGAGTAGGGATGAGATGGACTCAACGGAATTGACGGGTAC	5	0.11101243339253995	No Hit
CGAGTAGGGCTGAGATGGACTCAAAGGAATTGACGGGTAC	5	0.11101243339253995	No Hit
CGAGTAGGGATGAGATGGACTTACAGGAATTGACCGGTAC	5	0.11101243339253995	No Hit
CGAGTAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGA	5	0.11101243339253995	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTCAAA	35	7.0839815E-7	34.534092	19
ACTTACA	20	0.0023151317	34.534092	19
CCTCAAA	35	7.0839815E-7	34.534092	19
GACTCAA	35	7.0839815E-7	34.534092	18
GACTTAC	20	0.0023151317	34.534092	18
ACTTAAA	30	1.0574689E-5	34.53409	19
GACTTAA	30	1.0574689E-5	34.53409	18
GATGGAC	85	0.0	34.14607	14
ATGGACT	85	0.0	34.14607	15
AAAGGAA	65	0.0	34.14607	23
TGGACTT	50	2.3101165E-10	34.14607	16
CTTAAAG	25	1.6799787E-4	34.14607	20
AAGGAAT	65	0.0	34.14607	24
GGAATTG	65	0.0	34.14607	26
CAAATGA	30	1.1457623E-5	34.14607	22
TAAAGGA	25	1.6799787E-4	34.14607	22
TTAAAGG	25	1.6799787E-4	34.14607	21
CTCAAAT	30	1.1457623E-5	34.14607	20
AGGAATT	70	0.0	34.146065	25
TCAAAGG	40	5.230322E-8	34.146065	21
>>END_MODULE