FastQCFastQC Report
Fri 17 Jun 2016
SRR1526127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123
Sequences flagged as poor quality0
Sequence length40
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT353.1166518254674975No Hit
CGAGTAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG211.8699910952804988No Hit
CGAGTAGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT80.7123775601068566No Hit
CGAGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT60.5342831700801425No Hit
CGAGTAGGGATTGACAGTCTTGCATTTTGTTTTGTCTCGT50.44523597506678536No Hit
CGAGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.44523597506678536No Hit
CGAGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.3561887800534283No Hit
CGAGTAGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG40.3561887800534283No Hit
CGAGTAGGGTCCCAGATCCTCTTCTGAGATGAGTTTTTGT30.26714158504007124No Hit
CGAGTAGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT30.26714158504007124No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC30.26714158504007124No Hit
CGAGTAGGGTAACAGATCCTCTTCTGAGATGAGTTTTTGT30.26714158504007124No Hit
CGAGTAGGGTCACAGATCCTGCTCGTATGCCGTCTTCTGC30.26714158504007124TruSeq Adapter, Index 20 (95% over 23bp)
CGAGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.26714158504007124No Hit
CGAGTAGGGAGGTGGTGGACAGTGCCTACGAGGTGATCAA30.26714158504007124No Hit
CGAGTAGGGGTGCTCGAGTAGGGGAAAAAAAAAAAAAAAA20.17809439002671415No Hit
CGAGTAGGGAGATATTTGCCACCTCAGACTGTTGATCATA20.17809439002671415No Hit
CGAGTAGGGACTTTTAGGCAAAAAAAAAAAAAAAAAAAAA20.17809439002671415No Hit
CGAGTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT20.17809439002671415No Hit
CGAGTAGGGTCCCCGATCCTCTTCTGAGATGAGTTTTTGT20.17809439002671415No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGATGCCAGACCTC20.17809439002671415No Hit
CGAGTAGGGACTGCAGCTCTTTTCAGTTTTCGCTATACCT20.17809439002671415No Hit
CGAGTAGGGAACTATAGTTTTTCTTAGGTCTTTGTTTACT20.17809439002671415No Hit
CGAGTAGGGACATTTATCCTTTAAAAAAATGAAGTCTTAA20.17809439002671415No Hit
CGAGTAGGGTCACAGATCCTCTTCTGAGATGCGTTTTTGT20.17809439002671415No Hit
CGAGTAGGGTCACAGATACTCTTCTGAGATGAGTTTTTGT20.17809439002671415No Hit
CGAGTAGGGGTTTTCTAATTTAATTTAGTTAGATTCTAAT20.17809439002671415No Hit
CGAGTAGGGATCTATTGGGTTTGCCTTTTTGACTCATTTT20.17809439002671415No Hit
CGAGTAGGGGAGTTTTAGAAAAAAATAAATGTTCCATTTT20.17809439002671415No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGTCACAGATCCTC20.17809439002671415No Hit
CGAGTAGGGGATTCCGGAGAGGGAGCCTGAGAAACGGCTA20.17809439002671415No Hit
CGAGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA20.17809439002671415No Hit
CGAGTAGGGGTTTCTCTAGTGCAAGCGTGACACGGGTTCC20.17809439002671415No Hit
CGAGTAGGGATATAAGATCCTATCCAGAAGGAATAAAGGT20.17809439002671415No Hit
CGAGTAGGGTCCCAGATCCTCTTCTGAAGGAGATTGGGCG20.17809439002671415No Hit
CGAGTAGGGTCACAGATCATCTTCTGAGATGAGTTTTTGT20.17809439002671415No Hit
CGAGTAGGGGAGTGCAGTGCTCGAGTAGGGTCACAGATCC20.17809439002671415No Hit
CGAGTAGGGACTAATCCTAGCCCTCGCCCTACACAAATAT20.17809439002671415No Hit
CGAGTAGGGTGCAGTGCTCGAGCTGTTGGCGAGCCTCTGG20.17809439002671415No Hit
CGAGTAGGGGAGGAGAAGCCCGGTTTACGCTGATGACCGA20.17809439002671415No Hit
CGAGTAGGGAAATGATCTCCAGCTATACTATGAGCCTTAG20.17809439002671415No Hit
CGAGTAGGGGGGGAGAAACCTGAATGAGACTTGTTTCAAT20.17809439002671415No Hit
CGAGTAGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAA20.17809439002671415No Hit
CGAGTAGGGAACCCTACCTGGCACTGCATTGTGGGCCGAA20.17809439002671415No Hit
CGAGTAGGGTCATAGATCCTCTTCTGAGATGAGTTTTTGT20.17809439002671415No Hit
CGAGTAGGGAGTGAAAATGCGAATGGCTCATTAAATCAGT20.17809439002671415No Hit
CGAGTAGGGTCTCTTTCCGCTGGAGGAGTCTGGAGACGAC20.17809439002671415No Hit
CGAGTAGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT20.17809439002671415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGT305.372249E-634.0000044
GGGTCAC200.001748993234.07
GTCACAG200.001748993234.09
AGGGTCA200.001748993234.06
GGTCACA200.001748993234.08
GTAGGGA352.7420538E-734.04
TAGGGTC200.001748993234.05
TCACAGA200.001748993234.010
CACAGAT200.001748993234.011
AGTAGGG1200.031.1666683
GAGTAGG1200.031.1666682
CGAGTAG1200.031.1666681
GTAGGGG509.500218E-827.24
Produced by FastQC (version 0.11.5)