FastQCFastQC Report
Fri 17 Jun 2016
SRR1526126_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526126_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61895
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG19643.173115760562242No Hit
TGCAATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT8281.3377494143307214No Hit
TGCAATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC5860.9467646821229501No Hit
TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG4700.7593505129655062No Hit
TGCAATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC2740.4426851926649972No Hit
TGCAATGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT2460.39744728976492444No Hit
TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2320.37482833831488815No Hit
TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG2310.3732126989255998No Hit
TGCAATGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC2030.3279747960255271No Hit
TGCAATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT1720.27788997495758944No Hit
TGCAATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1620.26173358106470634No Hit
TGCAATGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC1550.2504241053396882No Hit
TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG1480.23911462961466998No Hit
TGCAATGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA1380.22295823572178688No Hit
TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC1110.1793359722110025No Hit
TGCAATGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT1080.17448905404313758No Hit
TGCAATGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT1000.16156393892883109No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC990.15994829953954276No Hit
TGCAATGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC980.15833266015025446No Hit
TGCAATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG960.15510138137167784No Hit
TGCAATGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG950.15348574198238954No Hit
TGCAATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA940.1518701025931012No Hit
TGCAATGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG920.1486388238145246No Hit
TGCAATGGGATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT880.14217626625737137No Hit
TGCAATGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC800.12925115114306485No Hit
TGCAATGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG760.12278859358591163No Hit
TGCAATGGGAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGA740.11955731480733499No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC690.11147911786089344No Hit
TGCAATGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC690.11147911786089344No Hit
TGCAATGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC690.11147911786089344No Hit
TGCAATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC680.10986347847160513No Hit
TGCAATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC680.10986347847160513No Hit
TGCAATGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG680.10986347847160513No Hit
TGCAATGGGACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGG650.1050165603037402No Hit
TGCAATGGGGCTTGCAATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAA640.1034009209144519No Hit
TGCAATGGGGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCC630.10178528152516358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGAA150.00803082550.56111541
CGGAATA150.00803082550.56111541
TTGTATC150.00808309750.478340
TGTTTAA150.00808309750.478338
GTATTGA150.00808309750.478340
GCGCTTG203.96204E-450.478338
TCGTCTC354.8054062E-850.478340
AGACCTA150.00808309750.478338
ACCTAAT150.00808309750.478340
GACCTAA150.00808309750.478339
GCATTGA451.200533E-1050.47829440
CTTCATC150.008109326550.4369937
AGCGCTT203.9781033E-450.4369937
TAGACCT150.008109326550.4369937
GGCGCAT451.2187229E-1050.43698537
TATGCTC505.456968E-1250.3134735
ACACTGC150.00818839850.3134735
GCGGATC150.00818839850.3134735
CTCCACG150.00826804150.19055644
TCCACGA150.00826804150.19055645