FastQCFastQC Report
Fri 17 Jun 2016
SRR1526125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3809
Sequences flagged as poor quality0
Sequence length40
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC130.3412969283276451No Hit
TGCAATGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC100.2625360987135731No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG70.18377526909950118No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG60.15752165922814387No Hit
TGCAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.15752165922814387No Hit
TGCAATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGG60.15752165922814387No Hit
TGCAATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT50.13126804935678654No Hit
TGCAATGGGATTGGAGGAGTGGCTAAAGAAACATATAAAT50.13126804935678654No Hit
TGCAATGGGGCCGAATAGCCTCTCCACCCCAGCGGCCGGA50.13126804935678654No Hit
TGCAATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT50.13126804935678654No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC50.13126804935678654No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC40.10501443948542925No Hit
TGCAATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT40.10501443948542925No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAG40.10501443948542925No Hit
TGCAATGGGGGGACCAATGTGCTGGGGAGGAAGGTCCTCT40.10501443948542925No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTG251.6740573E-434.0000045
ATGGGGC200.002455202634.0000045
ATGGGAT453.3342076E-934.0000045
ATGGGAG502.1645974E-1034.0000045
GGGGGGG251.6740573E-434.0000047
AATGGGT453.3342076E-934.0000044
AATGGGC200.002455202634.0000044
TGGGTGG200.002455202634.0000046
TGGGAGT251.6740573E-434.0000046
ATGGGGG850.034.05
TGGGGGG650.034.06
TGCAATG3850.033.1168821
GCAATGG3800.033.1052632
CAATGGG3600.033.0555573
AATGGGG1550.032.9032254
AATGGGA1400.032.7857174
ATGGGGA451.4269972E-730.2222235
TGGGAAG250.007323320527.26
ATGGGAA350.001203340724.2857155
GGGGGGC501.23001755E-523.8000017
Produced by FastQC (version 0.11.5)