##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526125_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3809
Sequences flagged as poor quality	0
Sequence length	40
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.101076398004725	34.0	30.0	37.0	4.0	38.0
2	25.37542662116041	29.0	17.0	35.0	4.0	38.0
3	24.31163034917301	28.0	16.0	33.0	4.0	36.0
4	25.441323181937516	30.0	17.0	35.0	4.0	38.0
5	21.870044631136782	23.0	12.0	32.0	4.0	36.0
6	26.1430821737989	30.0	21.0	35.0	4.0	38.0
7	27.087161984772905	33.0	23.0	36.0	4.0	38.0
8	26.0425308479916	31.0	19.0	35.0	4.0	38.0
9	26.71409818850092	32.0	22.0	36.0	4.0	38.0
10	21.362824888422157	23.0	10.0	32.0	4.0	36.0
11	23.908374901548964	28.0	13.0	34.0	4.0	37.0
12	24.057495405618273	29.0	13.0	34.0	4.0	37.0
13	24.53058545550013	30.0	15.0	35.0	4.0	37.0
14	24.613021790496195	30.0	13.0	35.0	4.0	37.0
15	22.521921764242585	27.0	4.0	33.0	4.0	36.0
16	19.88369650826989	22.0	4.0	31.0	4.0	35.0
17	20.82882646363875	25.0	4.0	33.0	4.0	36.0
18	20.342084536623787	24.0	4.0	32.0	4.0	36.0
19	17.745602520346548	17.0	4.0	30.0	4.0	34.0
20	19.187188238382777	22.0	4.0	31.0	4.0	35.0
21	16.38356524022053	14.0	4.0	28.0	4.0	34.0
22	16.771068521921766	15.0	4.0	30.0	4.0	34.0
23	16.879758466789184	15.0	4.0	30.0	4.0	34.0
24	16.23654502494093	13.0	4.0	29.0	4.0	34.0
25	15.700971383565241	8.0	4.0	30.0	4.0	34.0
26	13.29246521396692	4.0	4.0	25.0	4.0	33.0
27	13.26227356261486	4.0	4.0	25.0	4.0	33.0
28	13.056445261223418	4.0	4.0	25.0	4.0	33.0
29	12.311105276975583	4.0	4.0	23.0	4.0	32.0
30	11.362824888422159	4.0	4.0	19.0	4.0	31.0
31	11.526122341822001	4.0	4.0	21.0	4.0	32.0
32	11.096875820425309	4.0	4.0	19.0	4.0	32.0
33	10.974796534523497	4.0	4.0	19.0	4.0	31.0
34	10.597007088474665	4.0	4.0	17.0	4.0	31.0
35	10.40483066421633	4.0	4.0	16.0	4.0	31.0
36	10.770543449724338	4.0	4.0	15.0	4.0	34.0
37	10.741664478865845	4.0	4.0	15.0	4.0	34.0
38	10.465476503019165	4.0	4.0	10.0	4.0	33.0
39	10.214754528747703	4.0	4.0	4.0	4.0	32.0
40	10.084536623785771	4.0	4.0	4.0	4.0	32.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-21.483533008813914
1	2	-19.749075019861532
1	3	-19.50532105223116
1	4	-19.506067683876676
1	5	-16.78509393228494
1	6	-20.146436553459033
1	7	-20.831052125153246
1	8	-20.648807995999007
1	9	-20.438458015143404
1	10	-14.985263238634033
1	11	-16.979925224588143
1	12	-17.23909763769315
1	13	-18.618061744747134
1	14	-17.279235839067297
1	15	-15.09271168906
1	16	-14.787335853908889
1	17	-14.850024413788457
1	18	-14.114792323781089
1	19	-13.8564447412762
1	20	-13.382917831232433
1	21	-8.573467044815358
1	22	-9.915678859218184
1	23	-10.569503000120987
1	24	-8.60587340250262
1	25	-8.532903270262823
1	26	-6.442179293864686
1	27	-6.008829747031995
1	28	-5.452234469650199
1	29	-5.457950507669609
1	30	-5.404935825722343
1	31	-5.89082512369029
1	32	-5.057162188622861
1	33	-5.009670822311272
1	34	-3.82622982510623
1	35	-4.866056477854232
1	36	-4.320471351370227
1	37	-5.009692554636378
1	38	-3.712294072968233
1	39	-5.084726041748515
1	40	-2.8620491867682896
4	1	-11.710805736086641
4	2	-9.021802292588806
4	3	-15.778048324958434
4	4	-6.460613138422129
4	5	-3.557821205012214
4	6	-8.100982008004486
4	7	-11.558324852425972
4	8	-17.966989814180824
4	9	-17.66573074241613
4	10	-5.4398086931794865
4	11	-5.252652497315417
4	12	-4.511824910420422
4	13	-6.527152653838044
4	14	-7.142872202703661
4	15	-5.00180259815091
4	16	0.1672096006365642
4	17	-6.7136607774248205
4	18	-2.6147923237810886
4	19	1.5526461678147108
4	20	0.8443548960402936
4	21	-6.255285226633539
4	22	0.902502958963634
4	23	1.7486788180608315
4	24	-10.878600675229894
4	25	-10.714721452081005
4	26	-8.351270202955595
4	27	-8.55428429248654
4	28	-7.997689015104745
4	29	-8.003405053124155
4	30	-7.1322085529950705
4	31	-7.7999160327812
4	32	-7.238980370441043
4	33	-6.736943549584
4	34	-6.462593461469867
4	35	-6.320601932399686
4	36	-6.138653169552045
4	37	-6.100601645545469
4	38	-6.166839527513687
4	39	-5.630180587203061
4	40	-5.316594641313744
6	1	3.455860930580023
6	2	0.6448643740778621
6	3	2.2219516750415664
6	4	0.039386861577870746
6	5	4.608845461654454
6	6	7.73235132532885
6	7	5.775008480907363
6	8	5.866343519152508
6	9	-0.8323974090827981
6	10	11.560191306820514
6	11	3.414014169351251
6	12	10.488175089579578
6	13	12.13951401282862
6	14	6.6904611306296715
6	15	6.99819740184909
6	16	10.333876267303232
6	17	10.78633922257518
6	18	9.218541009552244
6	19	11.385979501148043
6	20	10.677688229373626
6	21	12.24471477336646
6	22	13.902502958963634
6	23	18.24867881806083
6	24	11.788065991436774
6	25	19.95194521458566
6	26	18.315396463711075
6	27	10.112382374180127
6	28	4.335644318228587
6	29	5.329928280209177
6	30	4.8677914470049295
6	31	-1.1332493661145335
6	32	8.427686296225625
6	33	3.9297231170826663
6	34	9.204073205196801
6	35	8.012731400933646
6	36	1.528013497114621
6	37	1.8993983544545312
6	38	1.4998271391529787
6	39	4.0364860794636055
6	40	4.350072025352922
7	1	4.789194263913359
7	2	7.603197707411194
7	3	9.596951675041566
7	4	8.78938686157787
7	5	4.567178794987786
7	6	8.149017991995514
7	7	2.3166751475740277
7	8	2.283010185819176
7	9	5.58426925758387
7	10	9.310191306820514
7	11	6.122347502684583
7	12	8.988175089579578
7	13	10.847847346161956
7	14	10.48212779729634
7	15	10.99819740184909
7	16	10.292209600636564
7	17	13.16133922257518
7	18	3.3852076762189114
7	19	6.427646167814711
7	20	7.844354896040294
7	21	9.36971477336646
7	22	9.777502958963634
7	23	11.248678818060831
7	24	13.496399324770106
7	25	13.535278547918995
7	26	6.523729797044405
7	27	6.195715707513459
7	28	6.502310984895255
7	29	12.121594946875845
7	30	4.6177914470049295
7	31	4.7000839672188
7	32	5.386019629558957
7	33	1.8880564504160002
7	34	1.4124065385301332
7	35	-2.4456019323996863
7	36	0.11134683044795501
7	37	-3.100601645545469
7	38	-3.1668395275136874
7	39	-4.130180587203061
7	40	-2.5665946413137437
9	1	6.880103354822452
9	2	10.523652252865741
9	3	9.8583153114052
9	4	11.130295952486964
9	5	6.578542431351423
9	6	7.035381628359147
9	7	9.350766056664934
9	8	9.80573745854645
9	9	9.197905621220237
9	10	12.014736761365967
9	11	7.838256593593673
9	12	6.306356907761394
9	13	4.745574618889229
9	14	3.4480368882054293
9	15	3.4527428563945435
9	16	5.1217550551820175
9	17	9.87724831348427
9	18	11.430662221673458
9	19	4.325373440541984
9	20	11.617082168767567
9	21	16.971987500639187
9	22	8.357048413509087
9	23	17.6123151816972
9	24	17.485035688406473
9	25	11.830733093373542
9	26	7.466911615226222
9	27	3.6275338893316427
9	28	2.4568564394407097
9	29	1.9056858559667536
9	30	4.4132459924594745
9	31	2.381902149036982
9	32	4.9428378113771405
9	33	0.08123826859781857
9	34	4.9010429021664965
9	35	4.133943522145769
9	36	7.406801375902502
9	37	7.899398354454531
9	38	4.378615017940858
9	39	5.188001230978758
9	40	4.228859904140801
10	1	3.0968865716056655
10	2	3.593582322795811
10	3	-2.08574063265074
10	4	4.424002246193254
10	5	-1.519359666550674
10	6	6.860556453533974
10	7	6.3647520706509475
10	8	7.3022409550499425
10	9	6.680423103737716
10	10	8.714037460666667
10	11	8.978116733453817
10	12	11.257405858810344
10	13	6.549770423085032
10	14	11.43405087421942
10	15	10.613582017233707
10	16	5.974901908328871
10	17	10.863262299498256
10	18	10.500592291603528
10	19	11.437261552430094
10	20	10.42127797296337
10	21	12.629330157981844
10	22	13.364041420502094
10	23	8.248678818060831
10	24	11.50601470938549
10	25	9.669893932534379
10	26	4.264114412429022
10	27	4.5995618613596125
10	28	5.156157138741408
10	29	9.611979562260462
10	30	4.6370222162356995
10	31	6.9693147364495704
10	32	2.0687119372512655
10	33	4.49382568118523
10	34	4.768175769299363
10	35	0.06401345221569876
10	36	-0.6771147080135833
10	37	-1.0236785686223921
10	38	-2.0899164505906107
10	39	0.3698194127969394
10	40	0.14494382022471797
12	1	7.789194263913359
12	2	12.978197707411194
12	3	11.221951675041566
12	4	-1.9606131384221293
12	5	13.942178794987786
12	6	12.399017991995514
12	7	11.441675147574028
12	8	12.533010185819176
12	9	11.83426925758387
12	10	15.560191306820514
12	11	14.747347502684583
12	12	14.488175089579578
12	13	14.472847346161956
12	14	14.35712779729634
12	15	14.99819740184909
12	16	16.667209600636564
12	17	17.78633922257518
12	18	14.885207676218911
12	19	18.05264616781471
12	20	19.344354896040294
12	21	2.2447147733664607
12	22	13.902502958963634
12	23	17.24867881806083
12	24	16.121399324770106
12	25	18.285278547918995
12	26	3.648729797044405
12	27	5.445715707513459
12	28	15.002310984895255
12	29	16.996594946875845
12	30	14.86779144700493
12	31	14.2000839672188
12	32	4.761019629558957
12	33	16.263056450416002
12	34	7.537406538530133
12	35	19.679398067600314
12	36	-6.138653169552045
12	37	-6.100601645545469
12	38	-6.166839527513687
12	39	-5.630180587203061
12	40	-5.316594641313744
13	1	-9.044139069419973
13	2	-17.355135625922138
13	3	-16.278048324958434
13	4	-19.79394647175546
13	5	-15.891154538345546
13	6	-15.600982008004486
13	7	-15.891658185759306
13	8	-15.13365648084749
13	9	-16.832397409082795
13	10	-14.773142026512819
13	11	-9.08598583064875
13	12	-9.345158243753756
13	13	-8.527152653838044
13	14	-8.309538869370327
13	15	-8.668469264817576
13	16	-5.999457066030102
13	17	-6.546994110758154
13	18	-5.281458990447755
13	19	-3.6140204988519553
13	20	-5.655645103959706
13	21	-1.7552852266335393
13	22	-7.597497041036366
13	23	-6.5846545152725025
13	24	-6.04526734189656
13	25	-10.714721452081005
13	26	-8.351270202955595
13	27	-8.55428429248654
13	28	-7.997689015104745
13	29	-8.003405053124155
13	30	-7.1322085529950705
13	31	-7.7999160327812
13	32	-7.238980370441043
13	33	-6.736943549584
13	34	-6.462593461469867
13	35	-6.320601932399686
13	36	-6.138653169552045
13	37	-6.100601645545469
13	38	-6.166839527513687
13	39	-5.630180587203061
13	40	-5.316594641313744
14	1	2.789194263913359
14	2	0.9781977074111943
14	3	-3.7780483249584336
14	4	8.03938686157787
14	5	-10.057821205012214
14	6	-1.600982008004486
14	7	6.441675147574028
14	8	3.533010185819176
14	9	4.83426925758387
14	10	11.560191306820514
14	11	-2.2526524973154167
14	12	6.488175089579578
14	13	2.472847346161956
14	14	4.357127797296339
14	15	8.99819740184909
14	16	10.667209600636564
14	17	13.78633922257518
14	18	14.885207676218911
14	19	10.05264616781471
14	20	15.344354896040294
14	21	14.24471477336646
14	22	13.902502958963634
14	23	12.248678818060831
14	24	15.121399324770106
14	25	11.285278547918995
14	26	9.648729797044405
14	27	17.445715707513457
14	28	-7.997689015104745
14	29	-8.003405053124155
14	30	-7.1322085529950705
14	31	-7.7999160327812
14	32	-7.238980370441043
14	33	-6.736943549584
14	34	-6.462593461469867
14	35	-6.320601932399686
14	36	-6.138653169552045
14	37	-6.100601645545469
14	38	-6.166839527513687
14	39	-5.630180587203061
14	40	-5.316594641313744
15	1	5.989194263913362
15	2	9.978197707411194
15	3	5.021951675041567
15	4	5.23938686157787
15	5	11.942178794987786
15	6	10.399017991995514
15	7	9.441675147574028
15	8	9.933010185819175
15	9	9.234269257583868
15	10	13.960191306820512
15	11	7.747347502684583
15	12	10.68817508957958
15	13	11.872847346161954
15	14	12.757127797296338
15	15	15.198197401849093
15	16	14.467209600636561
15	17	11.986339222575182
15	18	12.08520767621891
15	19	9.05264616781471
15	20	15.944354896040295
15	21	6.44471477336646
15	22	5.3025029589636326
15	23	5.048678818060832
15	24	6.921399324770107
15	25	7.485278547918995
15	26	1.4487297970444057
15	27	-1.1542842924865404
15	28	0.0023109848952547196
15	29	2.9965949468758453
15	30	2.26779144700493
15	31	2.8000839672188
15	32	3.561019629558958
15	33	-0.3369435495839994
15	34	0.13740653853013285
15	35	0.6793980676003137
15	36	0.061346830447954304
15	37	2.699398354454532
15	38	0.8331604724863126
15	39	1.969819412796939
15	40	1.6834053586862563
16	1	4.039194263913359
16	2	2.1031977074111943
16	3	5.846951675041566
16	4	5.039386861577871
16	5	-5.682821205012214
16	6	8.274017991995514
16	7	7.191675147574028
16	8	10.158010185819176
16	9	8.33426925758387
16	10	9.185191306820514
16	11	10.247347502684583
16	12	7.613175089579578
16	13	7.972847346161956
16	14	10.85712779729634
16	15	6.87319740184909
16	16	8.667209600636564
16	17	10.03633922257518
16	18	8.760207676218911
16	19	7.302646167814711
16	20	11.719354896040294
16	21	6.744714773366461
16	22	1.152502958963634
16	23	4.4986788180608315
16	24	3.746399324770106
16	25	2.7852785479189954
16	26	-1.351270202955595
16	27	-3.0542842924865408
16	28	-2.4976890151047453
16	29	-1.5034050531241547
16	30	-7.1322085529950705
16	31	-7.7999160327812
16	32	-7.238980370441043
16	33	-6.736943549584
16	34	-6.462593461469867
16	35	-6.320601932399686
16	36	-6.138653169552045
16	37	-6.100601645545469
16	38	-6.166839527513687
16	39	-5.630180587203061
16	40	-5.316594641313744
17	1	6.173809648528746
17	2	7.208966938180428
17	3	6.529643982733873
17	4	7.193233015424024
17	5	4.172948025757016
17	6	6.783633376610901
17	7	6.8262905321894145
17	8	6.302240955049946
17	9	6.988115411430023
17	10	7.86788361451282
17	11	6.978116733453813
17	12	6.642021243425731
17	13	8.011308884623496
17	14	7.8955893357578795
17	15	5.53665894031063
17	16	3.3595172929442576
17	17	7.478646914882873
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103	35	-0.12060193239968697
103	36	0.061346830447954304
103	37	0.09939835445453049
103	38	0.03316047248631193
103	39	0.5698194127969387
103	40	0.8834053586862556
104	1	5.622527597246695
104	2	3.9781977074111943
104	3	4.388618341708234
104	4	3.5393868615778707
104	5	3.275512128321118
104	6	0.7323513253288461
104	7	2.77500848090736
104	8	2.533010185819176
104	9	-8.999064075749462
104	10	-5.27314202651282
104	11	-3.7526524973154167
104	12	-4.511824910420422
104	13	-1.693819320504712
104	14	-3.476205536036993
104	15	-0.0018025981509097733
104	16	-1.3327903993634358
104	17	-2.3803274440914883
104	18	0.05187434288557924
104	19	-8.780687165518623
104	20	-9.48897843729304
104	21	-5.088618559966873
104	22	-6.4308303743697
104	23	-7.2513211819391685
104	24	-8.378600675229894
104	25	-7.3813881187476715
104	26	-7.351270202955595
104	27	-6.054284292486541
104	28	-4.831022348438078
104	29	-4.670071719790822
104	30	-4.2988752196617375
104	31	-3.2999160327811996
104	32	-5.238980370441043
104	33	-3.7369435495839998
104	34	-4.462593461469867
104	35	-5.320601932399686
104	36	-3.971986502885378
104	37	-4.100601645545469
104	38	-3.6668395275136874
104	39	-1.6301805872030606
104	40	-0.8165946413137437
105	1	-2.960805736086641
105	2	-0.5218022925888057
105	3	-2.5280483249584336
105	4	-1.7106131384221293
105	5	-2.557821205012214
105	6	-3.100982008004486
105	7	0.1916751475740277
105	8	1.033010185819176
105	9	-1.6657307424161303
105	10	-0.18980869317948645
105	11	1.4973475026845833
105	12	-0.7618249104204224
105	13	3.722847346161956
105	14	2.6071277972963394
105	15	0.9981974018490902
105	16	1.9172096006365642
105	17	-0.7136607774248205
105	18	-0.11479232378108861
105	19	4.052646167814711
105	20	-0.6556451039597064
105	21	-10.75528522663354
105	22	-12.097497041036366
105	23	-11.751321181939169
105	24	-10.878600675229894
105	25	-10.714721452081005
105	26	-8.351270202955595
105	27	-8.55428429248654
105	28	-7.997689015104745
105	29	-8.003405053124155
105	30	-7.1322085529950705
105	31	-7.7999160327812
105	32	-7.238980370441043
105	33	-6.736943549584
105	34	-6.462593461469867
105	35	-6.320601932399686
105	36	-6.138653169552045
105	37	-6.100601645545469
105	38	-6.166839527513687
105	39	-5.630180587203061
105	40	-5.316594641313744
106	1	3.789194263913359
106	2	6.311531040744526
106	3	4.555285008374899
106	4	-0.6272798050887971
106	5	-1.3911545383455461
106	6	3.732351325328846
106	7	4.441675147574028
106	8	4.199676852485844
106	9	4.83426925758387
106	10	-1.7731420265128186
106	11	5.080680836017915
106	12	7.154841756246245
106	13	9.13951401282862
106	14	7.6904611306296715
106	15	10.99819740184909
106	16	7.333876267303232
106	17	8.119672555908512
106	18	3.5518743428855792
106	19	9.05264616781471
106	20	3.3443548960402936
106	21	5.578048106699793
106	22	-4.764163707703032
106	23	-3.0846545152725025
106	24	5.121399324770106
106	25	13.951945214585663
106	26	3.982063130377737
106	27	6.779049040846791
106	28	9.335644318228587
106	29	-5.336738386457488
106	30	-4.4655418863284035
106	31	-3.1332493661145335
106	32	-1.5723137037743768
106	33	-0.0702768829173337
106	34	0.20407320519679928
106	35	0.3460647342669798
106	36	4.194680163781289
106	37	4.232731687787865
106	38	4.166493805819647
106	39	4.703152746130273
106	40	5.01673869201959
107	1	-2.460805736086641
107	2	2.7281977074111943
107	3	2.2219516750415664
107	4	-4.460613138422129
107	5	0.192178794987786
107	6	-5.600982008004486
107	7	-6.808324852425972
107	8	-12.966989814180824
107	9	0.08426925758386972
107	10	1.5601913068205135
107	11	3.2473475026845833
107	12	4.238175089579578
107	13	-0.7771526538380442
107	14	1.1071277972963394
107	15	2.2481974018490902
107	16	4.167209600636564
107	17	5.0363392225751795
107	18	3.1352076762189114
107	19	2.5526461678147108
107	20	8.844354896040294
107	21	-0.005285226633539253
107	22	9.652502958963634
107	23	-2.7513211819391685
107	24	-0.12860067522989382
107	25	3.2852785479189954
107	26	-8.351270202955595
107	27	-8.55428429248654
107	28	-7.997689015104745
107	29	-8.003405053124155
107	30	-7.1322085529950705
107	31	-7.7999160327812
107	32	-7.238980370441043
107	33	-6.736943549584
107	34	-6.462593461469867
107	35	-6.320601932399686
107	36	-6.138653169552045
107	37	-6.100601645545469
107	38	-6.166839527513687
107	39	-5.630180587203061
107	40	-5.316594641313744
108	1	6.289194263913359
108	2	11.478197707411194
108	3	8.221951675041566
108	4	5.539386861577871
108	5	11.942178794987786
108	6	5.899017991995514
108	7	8.941675147574028
108	8	10.033010185819176
108	9	9.33426925758387
108	10	5.0601913068205135
108	11	11.747347502684583
108	12	8.488175089579578
108	13	8.972847346161956
108	14	10.85712779729634
108	15	-6.00180259815091
108	16	-2.832790399363436
108	17	-2.7136607774248205
108	18	-15.114792323781089
108	19	-13.94735383218529
108	20	-14.655645103959706
108	21	-10.75528522663354
108	22	-12.097497041036366
108	23	-11.751321181939169
108	24	-10.878600675229894
108	25	-10.714721452081005
108	26	-8.351270202955595
108	27	-8.55428429248654
108	28	-7.997689015104745
108	29	-8.003405053124155
108	30	-7.1322085529950705
108	31	-7.7999160327812
108	32	-7.238980370441043
108	33	-6.736943549584
108	34	-6.462593461469867
108	35	-6.320601932399686
108	36	-6.138653169552045
108	37	-6.100601645545469
108	38	-6.166839527513687
108	39	-5.630180587203061
108	40	-5.316594641313744
109	1	-26.21080573608664
109	2	-21.021802292588806
109	3	-19.778048324958434
109	4	-20.96061313842213
109	5	-18.057821205012214
109	6	-21.600982008004486
109	7	-22.558324852425972
109	8	-21.466989814180824
109	9	-22.16573074241613
109	10	-16.439808693179486
109	11	-19.252652497315417
109	12	-19.511824910420422
109	13	-19.527152653838044
109	14	-19.64287220270366
109	15	-17.00180259815091
109	16	-15.332790399363436
109	17	-16.21366077742482
109	18	-15.114792323781089
109	19	-13.94735383218529
109	20	-14.655645103959706
109	21	-10.75528522663354
109	22	-12.097497041036366
109	23	-11.751321181939169
109	24	-10.878600675229894
109	25	-10.714721452081005
109	26	-8.351270202955595
109	27	-8.55428429248654
109	28	-7.997689015104745
109	29	-8.003405053124155
109	30	-7.1322085529950705
109	31	-7.7999160327812
109	32	-7.238980370441043
109	33	-6.736943549584
109	34	-6.462593461469867
109	35	-6.320601932399686
109	36	-6.138653169552045
109	37	-6.100601645545469
109	38	-6.166839527513687
109	39	-5.630180587203061
109	40	-5.316594641313744
112	1	5.789194263913359
112	2	6.644864374077862
112	3	8.555285008374902
112	4	9.872720194911206
112	5	10.775512128321122
112	6	5.065684658662182
112	7	5.608341814240692
112	8	6.69967685248584
112	9	8.167602590917205
112	10	7.726857973487181
112	11	0.5806808360179154
112	12	1.6548417562462454
112	13	0.13951401282862363
112	14	2.6904611306296715
112	15	-1.0018025981509098
112	16	6.000542933969896
112	17	7.453005889241847
112	18	7.551874342885579
112	19	4.552646167814711
112	20	5.177688229373626
112	21	5.578048106699793
112	22	7.569169625630302
112	23	6.915345484727499
112	24	4.121399324770106
112	25	9.118611881252328
112	26	1.815396463711071
112	27	3.612382374180127
112	28	3.668977651561921
112	29	2.4965949468758453
112	30	5.3677914470049295
112	31	4.7000839672188
112	32	6.594352962892289
112	33	7.429723117082668
112	34	5.870739871863465
112	35	8.012731400933646
112	36	8.194680163781287
112	37	1.5660650211211973
112	38	4.499827139152979
112	39	2.5364860794636055
112	40	1.1834053586862563
114	1	-8.210805736086641
114	2	-5.021802292588806
114	3	-12.778048324958434
114	4	-11.96061313842213
114	5	-3.057821205012214
114	6	-4.600982008004486
114	7	3.4416751475740277
114	8	4.533010185819176
114	9	-4.16573074241613
114	10	-9.439808693179486
114	11	10.747347502684583
114	12	9.488175089579578
114	13	10.472847346161956
114	14	5.357127797296339
114	15	-8.00180259815091
114	16	10.667209600636564
114	17	9.78633922257518
114	18	-4.114792323781089
114	19	12.05264616781471
114	20	-14.655645103959706
114	21	-10.75528522663354
114	22	-12.097497041036366
114	23	-11.751321181939169
114	24	-10.878600675229894
114	25	-10.714721452081005
114	26	-8.351270202955595
114	27	-8.55428429248654
114	28	-7.997689015104745
114	29	-8.003405053124155
114	30	-7.1322085529950705
114	31	-7.7999160327812
114	32	-7.238980370441043
114	33	-6.736943549584
114	34	-6.462593461469867
114	35	-6.320601932399686
114	36	-6.138653169552045
114	37	-6.100601645545469
114	38	-6.166839527513687
114	39	-5.630180587203061
114	40	-5.316594641313744
118	1	7.789194263913359
118	2	8.978197707411194
118	3	4.221951675041566
118	4	12.03938686157787
118	5	0.942178794987786
118	6	12.399017991995514
118	7	11.441675147574028
118	8	12.533010185819176
118	9	9.83426925758387
118	10	17.560191306820514
118	11	14.747347502684583
118	12	6.488175089579578
118	13	1.4728473461619558
118	14	12.35712779729634
118	15	-11.00180259815091
118	16	3.667209600636564
118	17	15.78633922257518
118	18	17.88520767621891
118	19	14.05264616781471
118	20	18.344354896040294
118	21	23.24471477336646
118	22	18.902502958963634
118	23	22.24867881806083
118	24	21.121399324770106
118	25	23.285278547918995
118	26	4.648729797044405
118	27	4.445715707513459
118	28	26.002310984895253
118	29	22.996594946875845
118	30	26.86779144700493
118	31	26.200083967218802
118	32	23.76101962955896
118	33	13.263056450416
118	34	24.537406538530135
118	35	21.679398067600314
118	36	24.861346830447957
118	37	24.899398354454533
118	38	24.833160472486313
118	39	25.36981941279694
118	40	5.683405358686256
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	595.0
5	12.0
6	18.0
7	23.0
8	19.0
9	56.0
10	74.0
11	78.0
12	115.0
13	127.0
14	132.0
15	144.0
16	160.0
17	178.0
18	174.0
19	157.0
20	169.0
21	167.0
22	183.0
23	153.0
24	174.0
25	145.0
26	161.0
27	148.0
28	128.0
29	109.0
30	96.0
31	51.0
32	37.0
33	13.0
34	8.0
35	4.0
36	0.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	98.8427143608627	0.8153603366649131	0.21041557075223566	0.1315097317201473
8	97.89805570152392	0.36784025223331585	0.814503415659485	0.9196006305832896
9	96.92590646347871	1.9442984760903836	0.4729374671571204	0.6568575932737782
10	41.48070359674455	36.01995274350223	10.527697558414282	11.971646101338933
11	32.37070097138356	27.14623260698346	21.18666316618535	19.296403255447625
12	36.91257547912838	17.79994749278026	23.864531373063798	21.42294565502757
13	26.12234182200053	18.35127330007876	28.248884221580468	27.277500656340248
14	23.103176686794434	25.25597269624573	29.430296665791545	22.210553951168286
15	24.310842740876872	25.019690207403517	28.931478078235756	21.737988973483855
16	29.01023890784983	24.835914938304015	22.709372538724075	23.44447361512208
17	28.22263061170911	27.093725387240745	21.370438435284854	23.313205565765294
18	26.358624310842742	25.439747965345234	24.940929377789445	23.26069834602258
19	22.80747958914933	19.831445878324992	16.9080853305241	40.45298920200158
20	26.516145970070887	28.87897085849304	21.659228143869782	22.94565502756629
21	26.09608821212917	23.83827776319244	23.181937516408507	26.88369650826989
22	23.12943029666579	22.998162247309004	24.2320819112628	29.6403255447624
23	24.5996324494618	25.96482016277238	26.12234182200053	23.313205565765294
24	23.575741664478866	24.730900498818585	23.103176686794434	28.590181149908112
25	24.90803993694167	25.853914871255913	22.779821334734628	26.458223857067786
26	18.522427440633248	25.171503957783642	28.232189973614773	28.073878627968334
27	18.114990811236545	21.632974533998425	29.220267786820685	31.031766867944345
28	16.38225255972696	22.91940141769493	30.48044106064584	30.21790496193227
29	17.54201680672269	23.42436974789916	30.435924369747898	28.59768907563025
30	18.36573830793484	19.705727798213346	34.971098265895954	26.95743562795586
31	18.344283837056505	23.705650459921156	36.60972404730617	21.340341655716163
32	20.66701680672269	19.012605042016805	34.92647058823529	25.39390756302521
33	19.148936170212767	19.910690832676647	36.74809561334384	24.192277383766744
34	15.14833289577317	20.189025991073773	44.26358624310843	20.39905487004463
35	13.872832369942195	22.700998423541776	41.749868628481345	21.67630057803468
36	14.428383705650461	22.75952693823916	40.68331143232589	22.128777923784494
37	13.256180957390848	22.461862177801155	39.058390320883746	25.223566543924253
38	14.600840336134455	21.79621848739496	40.15231092436975	23.45063025210084
39	16.206987128972944	21.644339374835827	39.138429209351195	23.01024428684003
40	14.386978209503805	23.076923076923077	40.43055920189026	22.105539511682856
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.0
12	4.0
13	4.0
14	6.0
15	8.0
16	19.5
17	31.0
18	31.0
19	34.0
20	37.0
21	46.0
22	55.0
23	55.0
24	67.5
25	80.0
26	83.5
27	87.0
28	87.0
29	87.0
30	87.0
31	120.0
32	153.0
33	153.0
34	163.0
35	173.0
36	198.5
37	224.0
38	224.0
39	236.5
40	249.0
41	238.0
42	227.0
43	227.0
44	231.0
45	235.0
46	220.0
47	205.0
48	205.0
49	201.5
50	198.0
51	219.0
52	240.0
53	240.0
54	233.0
55	226.0
56	236.0
57	246.0
58	246.0
59	258.0
60	270.0
61	278.5
62	287.0
63	287.0
64	227.5
65	168.0
66	156.0
67	144.0
68	144.0
69	114.5
70	85.0
71	64.5
72	44.0
73	44.0
74	35.0
75	26.0
76	18.0
77	10.0
78	10.0
79	8.0
80	6.0
81	4.0
82	2.0
83	2.0
84	1.5
85	1.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.18377526909950118
8	0.07876082961407194
9	0.07876082961407194
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.31504331845628775
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.07876082961407194
26	0.4988185875557889
27	0.0
28	0.0
29	0.026253609871357313
30	0.07876082961407194
31	0.10501443948542925
32	0.026253609871357313
33	0.05250721974271463
34	0.0
35	0.07876082961407194
36	0.10501443948542925
37	0.18377526909950118
38	0.026253609871357313
39	0.05250721974271463
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	3809.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.03806773431347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.9060773480663	92.09766342872146
2	2.1823204419889506	4.148070359674455
3	0.49723756906077343	1.4176949330532949
4	0.11049723756906078	0.420057757941717
5	0.13812154696132595	0.6563402467839329
6	0.08287292817679558	0.4725649776844316
7	0.027624309392265196	0.18377526909950118
8	0.0	0.0
9	0.0	0.0
>10	0.05524861878453039	0.6038330270412182
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	13	0.3412969283276451	No Hit
TGCAATGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC	10	0.2625360987135731	No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	7	0.18377526909950118	No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	6	0.15752165922814387	No Hit
TGCAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6	0.15752165922814387	No Hit
TGCAATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGG	6	0.15752165922814387	No Hit
TGCAATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT	5	0.13126804935678654	No Hit
TGCAATGGGATTGGAGGAGTGGCTAAAGAAACATATAAAT	5	0.13126804935678654	No Hit
TGCAATGGGGCCGAATAGCCTCTCCACCCCAGCGGCCGGA	5	0.13126804935678654	No Hit
TGCAATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.13126804935678654	No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC	5	0.13126804935678654	No Hit
TGCAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC	4	0.10501443948542925	No Hit
TGCAATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	4	0.10501443948542925	No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAG	4	0.10501443948542925	No Hit
TGCAATGGGGGGACCAATGTGCTGGGGAGGAAGGTCCTCT	4	0.10501443948542925	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.026253609871357313	0.0	0.0	0.0	0.0
16	0.026253609871357313	0.0	0.0	0.0	0.0
17	0.026253609871357313	0.0	0.0	0.0	0.0
18	0.026253609871357313	0.0	0.0	0.0	0.0
19	0.026253609871357313	0.0	0.0	0.0	0.0
20	0.026253609871357313	0.0	0.0	0.0	0.0
21	0.026253609871357313	0.0	0.0	0.0	0.0
22	0.026253609871357313	0.0	0.0	0.0	0.0
23	0.026253609871357313	0.0	0.0	0.0	0.0
24	0.026253609871357313	0.0	0.0	0.0	0.0
25	0.026253609871357313	0.0	0.0	0.0	0.0
26	0.026253609871357313	0.0	0.0	0.0	0.0
27	0.026253609871357313	0.0	0.0	0.0	0.0
28	0.026253609871357313	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGTG	25	1.6740573E-4	34.000004	5
ATGGGGC	20	0.0024552026	34.000004	5
ATGGGAT	45	3.3342076E-9	34.000004	5
ATGGGAG	50	2.1645974E-10	34.000004	5
GGGGGGG	25	1.6740573E-4	34.000004	7
AATGGGT	45	3.3342076E-9	34.000004	4
AATGGGC	20	0.0024552026	34.000004	4
TGGGTGG	20	0.0024552026	34.000004	6
TGGGAGT	25	1.6740573E-4	34.000004	6
ATGGGGG	85	0.0	34.0	5
TGGGGGG	65	0.0	34.0	6
TGCAATG	385	0.0	33.116882	1
GCAATGG	380	0.0	33.105263	2
CAATGGG	360	0.0	33.055557	3
AATGGGG	155	0.0	32.903225	4
AATGGGA	140	0.0	32.785717	4
ATGGGGA	45	1.4269972E-7	30.222223	5
TGGGAAG	25	0.0073233205	27.2	6
ATGGGAA	35	0.0012033407	24.285715	5
GGGGGGC	50	1.23001755E-5	23.800001	7
>>END_MODULE