FastQCFastQC Report
Fri 17 Jun 2016
SRR1526123_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526123_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2588
Sequences flagged as poor quality0
Sequence length40
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT692.6661514683153014No Hit
TGCAATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT441.7001545595054095No Hit
TGCAATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG271.0432766615146831No Hit
TGCAATGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT120.46367851622874806No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC90.34775888717156106No Hit
TGCAATGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG80.3091190108191654No Hit
TGCAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.23183925811437403No Hit
TGCAATGGGGCTTGCAATGGGTCACAGATCCTCTTCTGAA60.23183925811437403No Hit
TGCAATGGGGCTTGCAATGGGTCACAGATCCTCTTCTGAG60.23183925811437403No Hit
TGCAATGGGCTTTATGTTACTTTATGCTGCTGGCTCTTGT60.23183925811437403No Hit
TGCAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.23183925811437403No Hit
TGCAATGGGTCACAGACCCTCTTCTGAAGGAGATTGGGCG50.19319938176197837No Hit
TGCAATGGGTCACAAATCCTCTTCTGAGATGAGTTTTTGT50.19319938176197837No Hit
TGCAATGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC40.1545595054095827No Hit
TGCAATGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT40.1545595054095827No Hit
TGCAATGGGAGGTCAGTTATGCCTCCTTACCATTGAGCTG40.1545595054095827No Hit
TGCAATGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGA40.1545595054095827No Hit
TGCAATGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT40.1545595054095827No Hit
TGCAATGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG40.1545595054095827No Hit
TGCAATGGGATTTACAGTTGGTGGTCTAACCGGAATTGTT40.1545595054095827No Hit
TGCAATGGGATGTGTGCCTACTGTGTGATGAAAGGGATTA40.1545595054095827No Hit
TGCAATGGGTACCTGGTTAATCCTGCCAGTAGCATATGCT40.1545595054095827No Hit
TGCAATGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCT40.1545595054095827No Hit
TGCAATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA40.1545595054095827No Hit
TGCAATGGGGGTCCTTATTGAGTGGAATTCTATTTGATGC40.1545595054095827No Hit
TGCAATGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT40.1545595054095827No Hit
TGCAATGGGTCACAGATCCCCTTCTGAGATGAGTTTTTGT30.11591962905718702No Hit
TGCAATGGGGGCAGACTTGGCTGAGAGCATAATGAAGAAC30.11591962905718702No Hit
TGCAATGGGTACCTGGTTGATCCTGCCAGAAGCATATGCT30.11591962905718702No Hit
TGCAATGGGGGAGAGGGAGCCTGAGAAACGGCTACCACAT30.11591962905718702No Hit
TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGAT30.11591962905718702No Hit
TGCAATGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT30.11591962905718702No Hit
TGCAATGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC30.11591962905718702No Hit
TGCAATGGGATGCCATCTACCTTCTTCAACCTCACCATAG30.11591962905718702No Hit
TGCAATGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT30.11591962905718702No Hit
TGCAATGGGACTAATCCTAGCCCTAGCCCTACACAAATAT30.11591962905718702No Hit
TGCAATGGGACCCACTCCCGACCCGGGGAGGTAGTGACGA30.11591962905718702No Hit
TGCAATGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT30.11591962905718702No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG30.11591962905718702No Hit
TGCAATGGGATTTCCTTCTCTATTTTTCATCTGAGATACA30.11591962905718702No Hit
TGCAATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT30.11591962905718702No Hit
TGCAATGGGGGCTGAAGTGAGAAAAAAAGATGGCTTTTTA30.11591962905718702No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT200.002301313734.0000045
TTCTGAA200.002301313734.00000422
TGGGGTG251.5175357E-434.0000046
AATGGGC200.002301313734.0000044
ATGGGGT309.859781E-634.05
AATGGGA600.034.04
CAATGGG2600.031.3846153
TGCAATG2800.030.9642851
GCAATGG2750.030.9090922
AATGGGG1100.030.909094
ATGGGTC506.868504E-930.6000025
ATGGGGG506.868504E-930.6000025
TGGGTCA451.1195516E-730.2222216
AATGGGT703.6379788E-1229.1428574
GGGTCAC304.4316376E-428.3333327
GGAGATT250.00686959827.229
ATGGGGA250.00686959827.25
CTGAGAT250.00686959827.224
TTCTGAG250.00686959827.222
GATGAGT250.00686959827.228
Produced by FastQC (version 0.11.5)