##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526123_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2588 Sequences flagged as poor quality 0 Sequence length 40 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 17.94744976816074 27.0 4.0 30.0 4.0 33.0 2 10.92194744976816 4.0 4.0 17.0 4.0 26.0 3 9.284389489953632 4.0 4.0 13.0 4.0 21.0 4 10.90340030911901 4.0 4.0 17.0 4.0 26.0 5 11.149149922720246 4.0 4.0 15.0 4.0 29.0 6 11.056800618238022 4.0 4.0 19.0 4.0 26.0 7 12.464064914992273 4.0 4.0 24.0 4.0 30.0 8 11.83114374034003 4.0 4.0 22.0 4.0 28.0 9 11.426970633693973 4.0 4.0 22.0 4.0 28.0 10 10.852009273570324 4.0 4.0 19.0 4.0 27.0 11 12.38369397217929 4.0 4.0 24.0 4.0 33.0 12 12.244590417310665 4.0 4.0 24.0 4.0 33.0 13 11.09466769706337 4.0 4.0 18.0 4.0 31.0 14 11.631375579598146 4.0 4.0 20.0 4.0 33.0 15 11.254250386398764 4.0 4.0 19.0 4.0 32.0 16 9.597758887171562 4.0 4.0 11.0 4.0 29.0 17 9.204404945904173 4.0 4.0 10.0 4.0 26.0 18 8.821483771251932 4.0 4.0 9.0 4.0 26.0 19 8.732612055641422 4.0 4.0 9.0 4.0 26.0 20 8.900695517774343 4.0 4.0 7.0 4.0 27.0 21 7.9080370942812985 4.0 4.0 4.0 4.0 23.0 22 7.94435857805255 4.0 4.0 4.0 4.0 23.0 23 7.916924265842349 4.0 4.0 4.0 4.0 23.0 24 7.7136785162287484 4.0 4.0 4.0 4.0 22.0 25 7.921947449768161 4.0 4.0 4.0 4.0 23.0 26 8.125579598145286 4.0 4.0 4.0 4.0 25.0 27 8.226816074188562 4.0 4.0 4.0 4.0 26.0 28 8.430834621329211 4.0 4.0 4.0 4.0 27.0 29 7.795981452859351 4.0 4.0 4.0 4.0 23.0 30 8.010046367851622 4.0 4.0 4.0 4.0 24.0 31 8.676970633693973 4.0 4.0 4.0 4.0 29.0 32 8.607805255023184 4.0 4.0 4.0 4.0 29.0 33 8.370170015455951 4.0 4.0 4.0 4.0 28.0 34 8.585780525502319 4.0 4.0 4.0 4.0 30.0 35 8.293276661514684 4.0 4.0 4.0 4.0 29.0 36 8.370942812982998 4.0 4.0 4.0 4.0 30.0 37 8.185085007727976 4.0 4.0 4.0 4.0 30.0 38 8.104714064914992 4.0 4.0 4.0 4.0 29.0 39 7.969860896445131 4.0 4.0 4.0 4.0 29.0 40 7.900309119010819 4.0 4.0 4.0 4.0 29.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 14.669337979094074 1 2 8.624695121951218 1 3 8.060990127758421 1 4 15.72616144018583 1 5 0.6341753774680594 1 6 4.202395470383273 1 7 2.544076655052267 1 8 -6.186933797909408 1 9 3.86029326364692 1 10 -7.759262485481997 1 11 -8.1784262485482 1 12 -8.158681765389082 1 13 -7.059204413472704 1 14 -6.249956445993028 1 15 -6.928237514518001 1 16 -4.60081300813008 1 17 -3.985728803716608 1 18 -3.6204123112659703 1 19 -2.8880516840882695 1 20 -3.1778600464576074 1 21 -2.93927119628339 1 22 -2.9399825783972124 1 23 -2.6863966318234622 1 24 -2.775667828106852 1 25 -2.8378193960511036 1 26 -3.3428426248548195 1 27 -3.1515824622531943 1 28 -3.580139372822299 1 29 -3.1243466898954715 1 30 -3.1380662020905907 1 31 -3.5910278745644595 1 32 -3.5133565621370515 1 33 -3.1726771196283385 1 34 -3.325217770034844 1 35 -2.88558362369338 1 36 -2.73130081300813 1 37 -2.182520325203252 1 38 -2.3912601626016263 1 39 -2.07132694541231 1 40 -2.4717479674796756 2 1 -15.830662020905926 2 2 -9.375304878048782 2 3 -5.939009872241579 2 4 -7.77383855981417 2 5 -8.36582462253194 2 6 -8.297604529616727 2 7 -9.455923344947733 2 8 -9.186933797909408 2 9 -9.13970673635308 2 10 -7.759262485481997 2 11 -8.1784262485482 2 12 -8.158681765389082 2 13 -7.059204413472704 2 14 -6.249956445993028 2 15 -6.928237514518001 2 16 -4.60081300813008 2 17 -3.985728803716608 2 18 -3.6204123112659703 2 19 -2.8880516840882695 2 20 -3.1778600464576074 2 21 -2.93927119628339 2 22 -2.9399825783972124 2 23 -2.6863966318234622 2 24 -2.775667828106852 2 25 -2.8378193960511036 2 26 -3.3428426248548195 2 27 -3.1515824622531943 2 28 -3.580139372822299 2 29 -3.1243466898954715 2 30 -3.1380662020905907 2 31 -3.5910278745644595 2 32 -3.5133565621370515 2 33 -3.1726771196283385 2 34 -3.325217770034844 2 35 -2.88558362369338 2 36 -2.73130081300813 2 37 -2.182520325203252 2 38 -2.3912601626016263 2 39 -2.07132694541231 2 40 -2.4717479674796756 3 1 13.169337979094074 3 2 11.624695121951218 3 3 2.060990127758421 3 4 21.22616144018583 3 5 17.63417537746806 3 6 17.702395470383273 3 7 13.544076655052267 3 8 18.813066202090592 3 9 18.86029326364692 3 10 -7.759262485481997 3 11 -8.1784262485482 3 12 -8.158681765389082 3 13 -7.059204413472704 3 14 -6.249956445993028 3 15 -6.928237514518001 3 16 -4.60081300813008 3 17 -3.985728803716608 3 18 -3.6204123112659703 3 19 -2.8880516840882695 3 20 -3.1778600464576074 3 21 -2.93927119628339 3 22 -2.9399825783972124 3 23 -2.6863966318234622 3 24 -2.775667828106852 3 25 -2.8378193960511036 3 26 -3.3428426248548195 3 27 -3.1515824622531943 3 28 -3.580139372822299 3 29 -3.1243466898954715 3 30 -3.1380662020905907 3 31 -3.5910278745644595 3 32 -3.5133565621370515 3 33 -3.1726771196283385 3 34 -3.325217770034844 3 35 -2.88558362369338 3 36 -2.73130081300813 3 37 -2.182520325203252 3 38 -2.3912601626016263 3 39 -2.07132694541231 3 40 -2.4717479674796756 4 1 -15.830662020905926 4 2 -9.375304878048782 4 3 -5.939009872241579 4 4 -7.77383855981417 4 5 -8.36582462253194 4 6 -8.297604529616727 4 7 -9.455923344947733 4 8 -9.186933797909408 4 9 -9.13970673635308 4 10 -7.759262485481997 4 11 -8.1784262485482 4 12 -8.158681765389082 4 13 -7.059204413472704 4 14 -6.249956445993028 4 15 -6.928237514518001 4 16 -4.60081300813008 4 17 -3.985728803716608 4 18 -3.6204123112659703 4 19 -2.8880516840882695 4 20 -3.1778600464576074 4 21 -2.93927119628339 4 22 -2.9399825783972124 4 23 -2.6863966318234622 4 24 -2.775667828106852 4 25 -2.8378193960511036 4 26 -3.3428426248548195 4 27 -3.1515824622531943 4 28 -3.580139372822299 4 29 -3.1243466898954715 4 30 -3.1380662020905907 4 31 -3.5910278745644595 4 32 -3.5133565621370515 4 33 -3.1726771196283385 4 34 -3.325217770034844 4 35 -2.88558362369338 4 36 -2.73130081300813 4 37 -2.182520325203252 4 38 -2.3912601626016263 4 39 -2.07132694541231 4 40 -2.4717479674796756 5 1 13.169337979094074 5 2 19.62469512195122 5 3 10.060990127758421 5 4 -1.7738385598141697 5 5 15.63417537746806 5 6 15.702395470383273 5 7 7.544076655052267 5 8 14.813066202090592 5 9 16.86029326364692 5 10 19.240737514518003 5 11 13.8215737514518 5 12 -3.1586817653890815 5 13 20.940795586527294 5 14 -0.24995644599302835 5 15 20.071762485481997 5 16 7.39918699186992 5 17 11.014271196283392 5 18 17.37958768873403 5 19 9.11194831591173 5 20 13.822139953542393 5 21 3.06072880371661 5 22 16.060017421602787 5 23 21.313603368176537 5 24 26.22433217189315 5 25 21.162180603948897 5 26 22.657157375145182 5 27 22.848417537746805 5 28 25.4198606271777 5 29 15.875653310104529 5 30 25.86193379790941 5 31 27.40897212543554 5 32 25.48664343786295 5 33 27.82732288037166 5 34 27.674782229965157 5 35 26.11441637630662 5 36 -2.73130081300813 5 37 -2.182520325203252 5 38 -2.3912601626016263 5 39 -2.07132694541231 5 40 -2.4717479674796756 6 1 13.169337979094074 6 2 5.624695121951218 6 3 -0.939009872241579 6 4 13.22616144018583 6 5 20.63417537746806 6 6 4.702395470383273 6 7 8.544076655052267 6 8 13.813066202090592 6 9 14.86029326364692 6 10 9.240737514518003 6 11 7.821573751451799 6 12 17.841318234610917 6 13 21.940795586527294 6 14 -4.249956445993028 6 15 19.071762485481997 6 16 24.399186991869918 6 17 25.014271196283392 6 18 -3.6204123112659703 6 19 -2.8880516840882695 6 20 -3.1778600464576074 6 21 -2.93927119628339 6 22 -2.9399825783972124 6 23 -2.6863966318234622 6 24 -2.775667828106852 6 25 -2.8378193960511036 6 26 -3.3428426248548195 6 27 -3.1515824622531943 6 28 -3.580139372822299 6 29 -3.1243466898954715 6 30 -3.1380662020905907 6 31 -3.5910278745644595 6 32 -3.5133565621370515 6 33 -3.1726771196283385 6 34 -3.325217770034844 6 35 -2.88558362369338 6 36 -2.73130081300813 6 37 -2.182520325203252 6 38 -2.3912601626016263 6 39 -2.07132694541231 6 40 -2.4717479674796756 7 1 -0.030662020905925402 7 2 0.4246951219512187 7 3 -1.7390098722415797 7 4 4.22616144018583 7 5 -1.765824622531941 7 6 -7.097604529616727 7 7 -8.455923344947733 7 8 -5.786933797909407 7 9 -3.3397067363530795 7 10 -3.7592624854819974 7 11 -6.578426248548201 7 12 -4.558681765389082 7 13 2.5407955865272953 7 14 -0.849956445993028 7 15 2.071762485481999 7 16 1.7991869918699201 7 17 2.4142711962833925 7 18 1.579587688734029 7 19 1.5119483159117308 7 20 1.8221399535423926 7 21 6.06072880371661 7 22 4.060017421602788 7 23 5.313603368176538 7 24 3.6243321718931485 7 25 6.162180603948896 7 26 5.6571573751451805 7 27 6.448417537746805 7 28 2.419860627177701 7 29 7.275653310104529 7 30 1.0619337979094086 7 31 2.00897212543554 7 32 2.086643437862948 7 33 2.427322880371661 7 34 2.2747822299651554 7 35 1.5144163763066203 7 36 2.0686991869918705 7 37 2.6174796747967486 7 38 2.4087398373983744 7 39 2.92867305458769 7 40 2.328252032520325 8 1 6.169337979094074 8 2 -8.708638211382116 8 3 -4.772343205574912 8 4 -3.6071718931475036 8 5 -4.1991579558652745 8 6 -6.13093786295006 8 7 -7.6225900116144 8 8 -7.186933797909408 8 9 -6.973040069686413 8 10 -7.425929152148664 8 11 -4.178426248548201 8 12 -3.8253484320557476 8 13 -2.7258710801393704 8 14 -1.7499564459930284 8 15 -1.594904181184667 8 16 -4.60081300813008 8 17 -3.985728803716608 8 18 -3.6204123112659703 8 19 -2.8880516840882695 8 20 -3.1778600464576074 8 21 -2.93927119628339 8 22 -2.9399825783972124 8 23 -2.6863966318234622 8 24 -2.775667828106852 8 25 -2.8378193960511036 8 26 -3.3428426248548195 8 27 -3.1515824622531943 8 28 -3.580139372822299 8 29 -3.1243466898954715 8 30 -3.1380662020905907 8 31 -3.5910278745644595 8 32 -3.5133565621370515 8 33 -3.1726771196283385 8 34 -3.325217770034844 8 35 -2.88558362369338 8 36 -2.73130081300813 8 37 -2.182520325203252 8 38 -2.3912601626016263 8 39 -2.07132694541231 8 40 -2.4717479674796756 9 1 7.669337979094074 9 2 3.124695121951218 9 3 3.310990127758421 9 4 -1.7738385598141697 9 5 -2.6158246225319406 9 6 6.202395470383273 9 7 14.794076655052267 9 8 3.3130662020905923 9 9 5.61029326364692 9 10 4.990737514518003 9 11 17.3215737514518 9 12 22.591318234610917 9 13 11.690795586527296 9 14 4.500043554006972 9 15 8.821762485482 9 16 9.64918699186992 9 17 6.014271196283392 9 18 8.12958768873403 9 19 3.6119483159117305 9 20 6.0721399535423926 9 21 7.56072880371661 9 22 8.810017421602787 9 23 2.0636033681765378 9 24 8.724332171893149 9 25 7.162180603948896 9 26 9.65715737514518 9 27 6.598417537746806 9 28 12.669860627177702 9 29 13.375653310104529 9 30 14.861933797909408 9 31 13.65897212543554 9 32 14.236643437862949 9 33 14.57732288037166 9 34 13.424782229965157 9 35 13.86441637630662 9 36 14.51869918699187 9 37 9.567479674796747 9 38 12.358739837398375 9 39 3.42867305458769 9 40 10.278252032520324 10 1 9.169337979094074 10 2 2.124695121951218 10 3 5.060990127758421 10 4 1.2261614401858303 10 5 7.134175377468059 10 6 2.202395470383273 10 7 9.044076655052267 10 8 14.313066202090592 10 9 11.86029326364692 10 10 7.240737514518003 10 11 13.3215737514518 10 12 19.341318234610917 10 13 18.940795586527294 10 14 8.750043554006972 10 15 18.071762485481997 10 16 12.89918699186992 10 17 12.014271196283392 10 18 23.87958768873403 10 19 24.111948315911732 10 20 21.322139953542393 10 21 20.06072880371661 10 22 12.060017421602787 10 23 19.813603368176537 10 24 13.224332171893149 10 25 15.162180603948897 10 26 22.157157375145182 10 27 16.848417537746805 10 28 23.4198606271777 10 29 18.37565331010453 10 30 17.36193379790941 10 31 23.90897212543554 10 32 24.98664343786295 10 33 22.32732288037166 10 34 20.674782229965157 10 35 20.11441637630662 10 36 22.26869918699187 10 37 19.817479674796747 10 38 19.608739837398375 10 39 25.92867305458769 10 40 21.528252032520324 11 1 9.669337979094074 11 2 1.624695121951218 11 3 -2.939009872241579 11 4 0.2261614401858303 11 5 2.1341753774680594 11 6 -0.7976045296167271 11 7 2.544076655052267 11 8 5.313066202090592 11 9 5.36029326364692 11 10 6.740737514518003 11 11 5.321573751451799 11 12 1.3413182346109185 11 13 -1.0592044134727043 11 14 0.7500435540069716 11 15 0.07176248548199915 11 16 4.39918699186992 11 17 1.0142711962833921 11 18 2.3795876887340297 11 19 2.6119483159117305 11 20 4.8221399535423926 11 21 0.060728803716609825 11 22 1.0600174216027876 11 23 4.813603368176538 11 24 0.22433217189314814 11 25 9.662180603948897 11 26 7.6571573751451805 11 27 0.8484175377468057 11 28 7.419860627177701 11 29 2.8756533101045285 11 30 5.861933797909409 11 31 5.9089721254355405 11 32 4.4866434378629485 11 33 9.32732288037166 11 34 10.674782229965157 11 35 11.61441637630662 11 36 11.76869918699187 11 37 12.317479674796747 11 38 7.108739837398374 11 39 5.92867305458769 11 40 12.028252032520324 12 1 10.169337979094074 12 2 13.624695121951218 12 3 6.060990127758421 12 4 11.22616144018583 12 5 8.13417537746806 12 6 8.202395470383273 12 7 16.544076655052265 12 8 15.313066202090592 12 9 15.36029326364692 12 10 10.740737514518003 12 11 14.3215737514518 12 12 14.341318234610918 12 13 17.440795586527294 12 14 12.250043554006972 12 15 11.571762485482 12 16 1.3991869918699198 12 17 9.514271196283392 12 18 6.87958768873403 12 19 16.111948315911732 12 20 18.822139953542393 12 21 11.56072880371661 12 22 8.560017421602787 12 23 11.313603368176537 12 24 15.224332171893149 12 25 16.162180603948897 12 26 21.657157375145182 12 27 25.848417537746805 12 28 25.9198606271777 12 29 15.875653310104529 12 30 26.36193379790941 12 31 22.40897212543554 12 32 20.48664343786295 12 33 5.8273228803716615 12 34 11.674782229965157 12 35 7.11441637630662 12 36 11.76869918699187 12 37 6.817479674796748 12 38 6.608739837398374 12 39 12.42867305458769 12 40 12.028252032520324 13 1 3.502671312427406 13 2 -3.041971544715448 13 3 4.227656794425087 13 4 -1.6071718931475036 13 5 2.9675087108013933 13 6 -4.297604529616727 13 7 7.210743321718935 13 8 6.97973286875726 13 9 9.36029326364692 13 10 8.90740418118467 13 11 10.3215737514518 13 12 9.341318234610918 13 13 5.940795586527296 13 14 1.7500435540069716 13 15 5.738429152148667 13 16 1.5658536585365859 13 17 0.6809378629500582 13 18 4.87958768873403 13 19 4.445281649245064 13 20 2.3221399535423926 13 21 2.06072880371661 13 22 3.0600174216027876 13 23 2.8136033681765378 13 24 4.890998838559814 13 25 2.8288472706155625 13 26 3.9904907084785144 13 27 8.515084204413473 13 28 7.253193960511035 13 29 4.708986643437862 13 30 3.195267131242743 13 31 8.075638792102207 13 32 10.486643437862949 13 33 7.9939895470383275 13 34 9.174782229965157 13 35 7.281083042973286 13 36 10.102032520325203 13 37 6.150813008130082 13 38 7.27540650406504 13 39 8.262006387921023 13 40 12.194918699186992 14 1 9.169337979094074 14 2 9.624695121951218 14 3 0.06099012775842105 14 4 -1.7738385598141697 14 5 6.634175377468059 14 6 5.702395470383273 14 7 9.544076655052267 14 8 15.813066202090592 14 9 13.86029326364692 14 10 15.240737514518003 14 11 11.8215737514518 14 12 17.841318234610917 14 13 5.940795586527296 14 14 16.75004355400697 14 15 22.071762485481997 14 16 12.39918699186992 14 17 10.014271196283392 14 18 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-9.13970673635308 112 10 -7.759262485481997 112 11 -8.1784262485482 112 12 -8.158681765389082 112 13 -7.059204413472704 112 14 -6.249956445993028 112 15 -6.928237514518001 112 16 -4.60081300813008 112 17 -3.985728803716608 112 18 -3.6204123112659703 112 19 -2.8880516840882695 112 20 -3.1778600464576074 112 21 -2.93927119628339 112 22 -2.9399825783972124 112 23 -2.6863966318234622 112 24 -2.775667828106852 112 25 -2.8378193960511036 112 26 -3.3428426248548195 112 27 -3.1515824622531943 112 28 -3.580139372822299 112 29 -3.1243466898954715 112 30 -3.1380662020905907 112 31 -3.5910278745644595 112 32 -3.5133565621370515 112 33 -3.1726771196283385 112 34 -3.325217770034844 112 35 -2.88558362369338 112 36 -2.73130081300813 112 37 -2.182520325203252 112 38 -2.3912601626016263 112 39 -2.07132694541231 112 40 -2.4717479674796756 114 1 -15.830662020905926 114 2 -9.375304878048782 114 3 -5.939009872241579 114 4 -7.77383855981417 114 5 -8.36582462253194 114 6 -8.297604529616727 114 7 -9.455923344947733 114 8 -9.186933797909408 114 9 -9.13970673635308 114 10 -7.759262485481997 114 11 -8.1784262485482 114 12 -8.158681765389082 114 13 -7.059204413472704 114 14 -6.249956445993028 114 15 -6.928237514518001 114 16 -4.60081300813008 114 17 -3.985728803716608 114 18 -3.6204123112659703 114 19 -2.8880516840882695 114 20 -3.1778600464576074 114 21 -2.93927119628339 114 22 -2.9399825783972124 114 23 -2.6863966318234622 114 24 -2.775667828106852 114 25 -2.8378193960511036 114 26 -3.3428426248548195 114 27 -3.1515824622531943 114 28 -3.580139372822299 114 29 -3.1243466898954715 114 30 -3.1380662020905907 114 31 -3.5910278745644595 114 32 -3.5133565621370515 114 33 -3.1726771196283385 114 34 -3.325217770034844 114 35 -2.88558362369338 114 36 -2.73130081300813 114 37 -2.182520325203252 114 38 -2.3912601626016263 114 39 -2.07132694541231 114 40 -2.4717479674796756 115 1 -15.830662020905926 115 2 -9.375304878048782 115 3 -5.939009872241579 115 4 -7.77383855981417 115 5 -8.36582462253194 115 6 -8.297604529616727 115 7 -9.455923344947733 115 8 -9.186933797909408 115 9 -9.13970673635308 115 10 -7.759262485481997 115 11 -8.1784262485482 115 12 -8.158681765389082 115 13 -7.059204413472704 115 14 -6.249956445993028 115 15 -6.928237514518001 115 16 -4.60081300813008 115 17 -3.985728803716608 115 18 -3.6204123112659703 115 19 -2.8880516840882695 115 20 -3.1778600464576074 115 21 -2.93927119628339 115 22 -2.9399825783972124 115 23 -2.6863966318234622 115 24 -2.775667828106852 115 25 -2.8378193960511036 115 26 -3.3428426248548195 115 27 -3.1515824622531943 115 28 -3.580139372822299 115 29 -3.1243466898954715 115 30 -3.1380662020905907 115 31 -3.5910278745644595 115 32 -3.5133565621370515 115 33 -3.1726771196283385 115 34 -3.325217770034844 115 35 -2.88558362369338 115 36 -2.73130081300813 115 37 -2.182520325203252 115 38 -2.3912601626016263 115 39 -2.07132694541231 115 40 -2.4717479674796756 116 1 -15.830662020905926 116 2 -9.375304878048782 116 3 -5.939009872241579 116 4 -7.77383855981417 116 5 -8.36582462253194 116 6 -8.297604529616727 116 7 -9.455923344947733 116 8 -9.186933797909408 116 9 -9.13970673635308 116 10 -7.759262485481997 116 11 -8.1784262485482 116 12 -8.158681765389082 116 13 -7.059204413472704 116 14 -6.249956445993028 116 15 -6.928237514518001 116 16 -4.60081300813008 116 17 -3.985728803716608 116 18 -3.6204123112659703 116 19 -2.8880516840882695 116 20 -3.1778600464576074 116 21 -2.93927119628339 116 22 -2.9399825783972124 116 23 -2.6863966318234622 116 24 -2.775667828106852 116 25 -2.8378193960511036 116 26 -3.3428426248548195 116 27 -3.1515824622531943 116 28 -3.580139372822299 116 29 -3.1243466898954715 116 30 -3.1380662020905907 116 31 -3.5910278745644595 116 32 -3.5133565621370515 116 33 -3.1726771196283385 116 34 -3.325217770034844 116 35 -2.88558362369338 116 36 -2.73130081300813 116 37 -2.182520325203252 116 38 -2.3912601626016263 116 39 -2.07132694541231 116 40 -2.4717479674796756 117 1 9.169337979094074 117 2 2.124695121951218 117 3 6.060990127758421 117 4 2.2261614401858303 117 5 9.63417537746806 117 6 2.202395470383273 117 7 -3.955923344947733 117 8 4.813066202090592 117 9 1.8602932636469198 117 10 1.2407375145180026 117 11 13.3215737514518 117 12 8.841318234610918 117 13 3.4407955865272957 117 14 3.2500435540069716 117 15 5.571762485481999 117 16 11.89918699186992 117 17 13.514271196283392 117 18 11.87958768873403 117 19 12.11194831591173 117 20 8.322139953542393 117 21 21.06072880371661 117 22 21.560017421602787 117 23 19.813603368176537 117 24 19.22433217189315 117 25 16.662180603948897 117 26 11.65715737514518 117 27 10.848417537746805 117 28 11.419860627177702 117 29 10.875653310104529 117 30 11.861933797909408 117 31 -3.5910278745644595 117 32 -3.5133565621370515 117 33 -3.1726771196283385 117 34 -3.325217770034844 117 35 -2.88558362369338 117 36 -2.73130081300813 117 37 -2.182520325203252 117 38 -2.3912601626016263 117 39 -2.07132694541231 117 40 -2.4717479674796756 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 1412.0 5 34.0 6 34.0 7 57.0 8 51.0 9 72.0 10 87.0 11 84.0 12 67.0 13 53.0 14 46.0 15 28.0 16 18.0 17 22.0 18 23.0 19 39.0 20 57.0 21 88.0 22 94.0 23 66.0 24 52.0 25 43.0 26 23.0 27 20.0 28 9.0 29 5.0 30 2.0 31 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 98.87814313346229 0.6189555125725338 0.3094777562862669 0.19342359767891684 8 98.14313346228239 0.7350096711798839 0.6963249516441006 0.425531914893617 9 97.60432766615146 0.5795981452859351 1.1978361669242659 0.6182380216383307 10 42.73570324574961 26.85471406491499 20.556414219474497 9.853168469860897 11 29.057187017001546 28.091190108191654 20.36321483771252 22.48840803709428 12 26.59451101662157 26.633165829145728 24.777734827986084 21.994588326246618 13 19.550909794812235 22.338366240805264 26.093689508323653 32.017034456058845 14 21.005802707930368 33.65570599613153 26.731141199226304 18.6073500967118 15 34.42812982998454 26.159196290571867 23.956723338485318 15.455950540958268 16 31.336939721792888 35.66460587326121 20.44049459041731 12.557959814528594 17 24.661770390413608 25.125628140703515 35.25318902203324 14.959412446849631 18 21.290571870170016 25.579598145285935 31.839258114374037 21.290571870170016 19 26.527455529775718 28.924980665119875 22.96983758700696 21.57772621809745 20 23.995363214837713 30.757341576506953 31.993817619783616 13.253477588871714 21 23.377125193199383 23.608964451313756 28.013910355486864 25.0 22 17.8902627511592 22.449768160741886 36.90108191653786 22.758887171561053 23 18.399690761499805 25.47352145342095 33.513722458446075 22.613065326633166 24 17.928902627511594 30.061823802163833 29.327666151468318 22.68160741885626 25 26.5661252900232 26.024748646558393 28.692962103634954 18.71616395978345 26 28.576967816983327 27.53005040713455 22.334238076773943 21.55874369910818 27 19.162141194724594 34.328937160589604 21.25678820791311 25.252133436772695 28 21.762659451101662 37.7657518361036 23.96598376497874 16.505604947816003 29 30.422972448583625 31.199068684516877 21.924718665114472 16.45324020178502 30 21.77107501933488 27.648878576952825 32.67594740912607 17.904098994586235 31 25.0 33.19165378670788 24.072642967542503 17.735703245749615 32 29.717163889965132 30.414567996900427 24.912824486633088 14.955443626501355 33 26.661514683153015 28.825347758887172 24.072642967542503 20.44049459041731 34 17.8516228748068 31.955177743431225 34.77588871715611 15.417310664605873 35 15.108191653786706 28.632148377125194 41.190108191653785 15.069551777434311 36 20.43343653250774 32.31424148606811 28.560371517027868 18.691950464396285 37 21.676970633693973 27.086553323029367 34.19629057187017 17.04018547140649 38 28.593508500772796 27.009273570324577 27.550231839258117 16.846986089644513 39 23.64760432766615 29.095826893353944 23.02936630602782 24.227202472952087 40 19.82225656877898 28.207109737248842 33.69397217928903 18.276661514683155 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 4.0 12 8.0 13 8.0 14 14.5 15 21.0 16 21.5 17 22.0 18 22.0 19 25.0 20 28.0 21 27.0 22 26.0 23 26.0 24 27.0 25 28.0 26 39.0 27 50.0 28 50.0 29 55.0 30 60.0 31 64.0 32 68.0 33 68.0 34 85.5 35 103.0 36 108.5 37 114.0 38 114.0 39 146.0 40 178.0 41 219.0 42 260.0 43 260.0 44 239.5 45 219.0 46 214.0 47 209.0 48 209.0 49 268.0 50 327.0 51 303.5 52 280.0 53 280.0 54 236.0 55 192.0 56 182.0 57 172.0 58 172.0 59 146.5 60 121.0 61 88.5 62 56.0 63 56.0 64 39.5 65 23.0 66 18.5 67 14.0 68 14.0 69 10.5 70 7.0 71 4.0 72 1.0 73 1.0 74 1.0 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.11591962905718702 8 0.11591962905718702 9 0.0 10 0.0 11 0.0 12 0.03863987635239567 13 0.19319938176197837 14 0.11591962905718702 15 0.0 16 0.0 17 0.03863987635239567 18 0.0 19 0.07727975270479134 20 0.0 21 0.0 22 0.0 23 0.03863987635239567 24 0.0 25 0.07727975270479134 26 0.34775888717156106 27 0.38639876352395675 28 0.03863987635239567 29 0.4250386398763524 30 0.07727975270479134 31 0.0 32 0.2704791344667697 33 0.0 34 0.0 35 0.0 36 0.1545595054095827 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 2588.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.36012364760433 #Duplication Level Percentage of deduplicated Percentage of total 1 94.27293064876957 81.41421947449768 2 3.8478747203579418 6.646058732612056 3 0.7158836689038032 1.8547140649149922 4 0.5816554809843401 2.009273570324575 5 0.0894854586129754 0.38639876352395675 6 0.22371364653243847 1.1591962905718702 7 0.0 0.0 8 0.0447427293064877 0.3091190108191654 9 0.0447427293064877 0.34775888717156106 >10 0.1342281879194631 3.2071097372488406 >50 0.0447427293064877 2.6661514683153014 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 69 2.6661514683153014 No Hit TGCAATGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 44 1.7001545595054095 No Hit TGCAATGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 27 1.0432766615146831 No Hit TGCAATGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT 12 0.46367851622874806 No Hit TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 9 0.34775888717156106 No Hit TGCAATGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG 8 0.3091190108191654 No Hit TGCAATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.23183925811437403 No Hit TGCAATGGGGCTTGCAATGGGTCACAGATCCTCTTCTGAA 6 0.23183925811437403 No Hit TGCAATGGGGCTTGCAATGGGTCACAGATCCTCTTCTGAG 6 0.23183925811437403 No Hit TGCAATGGGCTTTATGTTACTTTATGCTGCTGGCTCTTGT 6 0.23183925811437403 No Hit TGCAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6 0.23183925811437403 No Hit TGCAATGGGTCACAGACCCTCTTCTGAAGGAGATTGGGCG 5 0.19319938176197837 No Hit TGCAATGGGTCACAAATCCTCTTCTGAGATGAGTTTTTGT 5 0.19319938176197837 No Hit TGCAATGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGC 4 0.1545595054095827 No Hit TGCAATGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT 4 0.1545595054095827 No Hit TGCAATGGGAGGTCAGTTATGCCTCCTTACCATTGAGCTG 4 0.1545595054095827 No Hit TGCAATGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGA 4 0.1545595054095827 No Hit TGCAATGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT 4 0.1545595054095827 No Hit TGCAATGGGGAACGTCTGCCCTATCAACTTTCGATGGTAG 4 0.1545595054095827 No Hit TGCAATGGGATTTACAGTTGGTGGTCTAACCGGAATTGTT 4 0.1545595054095827 No Hit TGCAATGGGATGTGTGCCTACTGTGTGATGAAAGGGATTA 4 0.1545595054095827 No Hit TGCAATGGGTACCTGGTTAATCCTGCCAGTAGCATATGCT 4 0.1545595054095827 No Hit TGCAATGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCT 4 0.1545595054095827 No Hit TGCAATGGGCTCCTACTTGGATAACTGTGGTAATTCTAGA 4 0.1545595054095827 No Hit TGCAATGGGGGTCCTTATTGAGTGGAATTCTATTTGATGC 4 0.1545595054095827 No Hit TGCAATGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT 4 0.1545595054095827 No Hit TGCAATGGGTCACAGATCCCCTTCTGAGATGAGTTTTTGT 3 0.11591962905718702 No Hit TGCAATGGGGGCAGACTTGGCTGAGAGCATAATGAAGAAC 3 0.11591962905718702 No Hit TGCAATGGGTACCTGGTTGATCCTGCCAGAAGCATATGCT 3 0.11591962905718702 No Hit TGCAATGGGGGAGAGGGAGCCTGAGAAACGGCTACCACAT 3 0.11591962905718702 No Hit TGCAATGGGTACCTGGTTGATCCTGCCAGTAGCATATGAT 3 0.11591962905718702 No Hit TGCAATGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT 3 0.11591962905718702 No Hit TGCAATGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCAC 3 0.11591962905718702 No Hit TGCAATGGGATGCCATCTACCTTCTTCAACCTCACCATAG 3 0.11591962905718702 No Hit TGCAATGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT 3 0.11591962905718702 No Hit TGCAATGGGACTAATCCTAGCCCTAGCCCTACACAAATAT 3 0.11591962905718702 No Hit TGCAATGGGACCCACTCCCGACCCGGGGAGGTAGTGACGA 3 0.11591962905718702 No Hit TGCAATGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT 3 0.11591962905718702 No Hit TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 3 0.11591962905718702 No Hit TGCAATGGGATTTCCTTCTCTATTTTTCATCTGAGATACA 3 0.11591962905718702 No Hit TGCAATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.11591962905718702 No Hit TGCAATGGGGGCTGAAGTGAGAAAAAAAGATGGCTTTTTA 3 0.11591962905718702 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.07727975270479134 0.0 0.0 0.0 0.0 15 0.07727975270479134 0.0 0.0 0.0 0.0 16 0.07727975270479134 0.0 0.0 0.0 0.0 17 0.07727975270479134 0.0 0.0 0.0 0.0 18 0.07727975270479134 0.0 0.0 0.0 0.0 19 0.07727975270479134 0.0 0.0 0.0 0.0 20 0.07727975270479134 0.0 0.0 0.0 0.0 21 0.07727975270479134 0.0 0.0 0.0 0.0 22 0.07727975270479134 0.0 0.0 0.0 0.0 23 0.07727975270479134 0.0 0.0 0.0 0.0 24 0.07727975270479134 0.0 0.0 0.0 0.0 25 0.07727975270479134 0.0 0.0 0.0 0.0 26 0.07727975270479134 0.0 0.0 0.0 0.0 27 0.07727975270479134 0.0 0.0 0.0 0.0 28 0.07727975270479134 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGAT 20 0.0023013137 34.000004 5 TTCTGAA 20 0.0023013137 34.000004 22 TGGGGTG 25 1.5175357E-4 34.000004 6 AATGGGC 20 0.0023013137 34.000004 4 ATGGGGT 30 9.859781E-6 34.0 5 AATGGGA 60 0.0 34.0 4 CAATGGG 260 0.0 31.384615 3 TGCAATG 280 0.0 30.964285 1 GCAATGG 275 0.0 30.909092 2 AATGGGG 110 0.0 30.90909 4 ATGGGTC 50 6.868504E-9 30.600002 5 ATGGGGG 50 6.868504E-9 30.600002 5 TGGGTCA 45 1.1195516E-7 30.222221 6 AATGGGT 70 3.6379788E-12 29.142857 4 GGGTCAC 30 4.4316376E-4 28.333332 7 GGAGATT 25 0.006869598 27.2 29 ATGGGGA 25 0.006869598 27.2 5 CTGAGAT 25 0.006869598 27.2 24 TTCTGAG 25 0.006869598 27.2 22 GATGAGT 25 0.006869598 27.2 28 >>END_MODULE