FastQCFastQC Report
Fri 17 Jun 2016
SRR1526122_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526122_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15719
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG940.5980024174565812No Hit
TTCCGCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT650.4135123099433806No Hit
TTCCGCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC540.34353330364527007No Hit
TTCCGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT500.31808639226413893No Hit
TTCCGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC460.29263948088300784No Hit
TTCCGCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC440.27991602519244224No Hit
TTCCGCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA410.2608308416565939No Hit
TTCCGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC360.22902220243018004No Hit
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC310.19721356320376612No Hit
TTCCGCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT270.17176665182263504No Hit
TTCCGCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG260.16540492397735226No Hit
TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG260.16540492397735226No Hit
TTCCGCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC250.15904319613206946No Hit
TTCCGCGGGACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGG220.13995801259622112No Hit
TTCCGCGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG220.13995801259622112No Hit
TTCCGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC210.13359628475093838No Hit
TTCCGCGGGCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG210.13359628475093838No Hit
TTCCGCGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG200.12723455690565558No Hit
TTCCGCGGGCACACATCTAGATATCTTACTTTTACTCACTCCTCAGAAGCTCACCA190.1208728290603728No Hit
TTCCGCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG190.1208728290603728No Hit
TTCCGCGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG190.1208728290603728No Hit
TTCCGCGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG190.1208728290603728No Hit
TTCCGCGGGAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGA180.11451110121509002No Hit
TTCCGCGGGGTCAGCGTGTCCCACTATTCATGTCCCATCTCTGTGCATCCTGACTG180.11451110121509002No Hit
TTCCGCGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT180.11451110121509002No Hit
TTCCGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC180.11451110121509002No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC170.10814937336980723No Hit
TTCCGCGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC170.10814937336980723No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC170.10814937336980723No Hit
TTCCGCGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT160.10178764552452445No Hit
TTCCGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC160.10178764552452445No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTGA150.00781974750.7207840
CGGATTG150.00781974750.7207839
GCGGATT150.00781974750.7207838
GCTCGTC150.00781974750.7207838
CTCGTCT150.00781974750.7207839
TGGGCGG203.9025268E-450.3935535
GATTCCT150.00812669250.2315145
ATTCCTC150.00812669250.2315146
AAAACTC150.00823097150.07051550
CTCATTT150.00823097150.07051550
TCTTCCA150.00823097150.07051550
AAAAACT204.0291745E-450.07051549
CAAAAAC150.00823097150.07051548
TATCTGA150.00833624349.9105433
GCGGGGT1050.049.7515955
TTGCATT150.00844251349.75159519
GGATGGT150.00844251349.7515958
TTGTTCT150.00844251349.75159524
GGATGCA150.00844251349.7515958
CGGGGTT252.0536572E-549.7515956
Produced by FastQC (version 0.11.5)