##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526122_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15719 Sequences flagged as poor quality 0 Sequence length 56 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.3913734970418 39.0 38.0 40.0 36.0 40.0 2 38.14651059227686 39.0 38.0 40.0 35.0 40.0 3 37.934601437750494 39.0 38.0 40.0 35.0 40.0 4 37.96768242254596 39.0 38.0 40.0 35.0 40.0 5 36.756981996310195 39.0 36.0 39.0 33.0 40.0 6 37.05547426681087 39.0 38.0 39.0 33.0 40.0 7 37.28036134614161 39.0 38.0 39.0 34.0 40.0 8 37.30727145492716 39.0 38.0 39.0 34.0 40.0 9 37.443221578980854 39.0 38.0 39.0 35.0 40.0 10 36.080412239964375 39.0 35.0 39.0 31.0 40.0 11 34.572873592467715 38.0 33.0 39.0 26.0 40.0 12 33.954513645906225 38.0 32.0 39.0 22.0 40.0 13 33.65964756027737 38.0 32.0 39.0 21.0 40.0 14 34.15503530758954 38.0 33.0 39.0 23.0 40.0 15 33.1383675806349 38.0 32.0 39.0 18.0 40.0 16 32.526814682867865 36.0 30.0 39.0 19.0 40.0 17 31.685603409886124 36.0 28.0 38.0 15.0 40.0 18 30.85310770405242 35.0 26.0 38.0 13.0 40.0 19 32.05827342706279 36.0 29.0 38.0 18.0 40.0 20 32.60493670080794 36.0 30.0 38.0 20.0 40.0 21 32.69247407595903 37.0 30.0 39.0 19.0 40.0 22 31.97996055728736 36.0 29.0 39.0 16.0 40.0 23 32.60824479928748 37.0 30.0 39.0 18.0 40.0 24 31.930529931929513 36.0 29.0 39.0 16.0 40.0 25 32.39684458298874 36.0 30.0 39.0 18.0 40.0 26 32.85527069151982 37.0 31.0 39.0 18.0 40.0 27 33.15554424581716 38.0 32.0 39.0 20.0 40.0 28 33.14193014822826 37.0 32.0 39.0 21.0 40.0 29 32.58559704815828 37.0 31.0 39.0 18.0 40.0 30 32.42006488962402 36.0 31.0 39.0 17.0 40.0 31 33.18410840384249 38.0 33.0 39.0 19.0 40.0 32 33.01685857879 37.0 32.0 39.0 19.0 40.0 33 32.921178191996944 37.0 32.0 39.0 19.0 40.0 34 32.76016286023284 37.0 32.0 39.0 18.0 40.0 35 32.55512437177938 37.0 31.0 39.0 17.0 40.0 36 31.9167885997837 36.0 30.0 38.0 11.0 40.0 37 31.706915198167824 36.0 30.0 38.0 10.0 40.0 38 31.01310515936128 35.0 29.0 38.0 4.0 40.0 39 30.06800687066607 35.0 27.0 38.0 4.0 39.0 40 28.10490489216871 33.0 21.0 38.0 4.0 39.0 41 26.207010624085502 31.0 16.0 37.0 4.0 39.0 42 26.906927921623513 32.0 19.0 37.0 4.0 39.0 43 26.844901075132007 32.0 19.0 37.0 4.0 39.0 44 25.900375341942873 31.0 15.0 37.0 4.0 39.0 45 26.761435205801895 32.0 19.0 37.0 4.0 39.0 46 24.746612379922386 29.0 13.0 36.0 4.0 39.0 47 23.74355875055665 28.0 9.0 35.0 4.0 39.0 48 24.274126852853236 29.0 10.0 35.0 4.0 39.0 49 23.231185189897577 28.0 4.0 35.0 4.0 39.0 50 23.851835358483363 29.0 4.0 35.0 4.0 39.0 51 23.905146637826835 30.0 4.0 36.0 4.0 39.0 52 23.736369998091483 30.0 4.0 36.0 4.0 39.0 53 23.39391818817991 29.0 4.0 35.0 4.0 38.0 54 23.059673007188753 29.0 4.0 35.0 4.0 38.0 55 22.77231376041733 28.0 4.0 35.0 4.0 38.0 56 22.262866594567086 28.0 4.0 36.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.5627932743021589 1 2 1.3613233263722577 1 3 1.6943534472287993 1 4 1.5265485730978696 1 5 3.088593860898463 1 6 3.0538526960695975 1 7 2.4177389527026563 1 8 2.503911749802704 1 9 2.578302321025035 1 10 4.016037015718972 1 11 -0.22275550980491943 1 12 -5.985284569803451 1 13 -16.528033850064574 1 14 -5.054931205029405 1 15 2.7607157232045054 1 16 -0.44773200604128505 1 17 -5.844472106380135 1 18 -2.536660255548643 1 19 0.2481226794613498 1 20 -0.7915268216435187 1 21 7.5108269819089095 1 22 7.807943671365194 1 23 7.437042368489482 1 24 7.48250159493557 1 25 6.478746816055047 1 26 6.5692986561736255 1 27 7.4774173763163105 1 28 7.300189083797626 1 29 5.941241414341306 1 30 5.806382626131452 1 31 6.74889496582778 1 32 6.317769552132965 1 33 5.736822759111291 1 34 5.875632880780639 1 35 6.137489341987482 1 36 5.638525126314118 1 37 5.032230111549076 1 38 6.108098102281097 1 39 5.813097243015445 1 40 -10.200339729538543 1 41 0.487937248539577 1 42 0.5655502411519286 1 43 -10.621668154286429 1 44 -13.389610691440971 1 45 -1.6925688626750173 1 46 -4.801480930553101 1 47 -9.407662309615041 1 48 -4.609407717141391 1 49 -13.297345500976547 1 50 -4.776408457932476 1 51 3.125878479386497 1 52 2.9051301822868716 1 53 2.680211524866362 1 54 3.4983006806030232 1 55 2.662847002955978 1 56 6.90083856259864 2 1 0.4051845786499868 2 2 0.8142218770968981 2 3 1.063918664620104 2 4 1.1080703122283069 2 5 1.7516373391593305 2 6 2.3871860294029332 2 7 1.5880288077751246 2 8 1.7122450831360396 2 9 1.7830124659525666 2 10 2.9417616534001354 2 11 3.6649256496153697 2 12 -0.6284005118324352 2 13 0.5353719470368716 2 14 3.2168079254053765 2 15 2.621222969581318 2 16 2.838499878016684 2 17 4.615672821156096 2 18 2.626383222712228 2 19 4.2408763026497525 2 20 3.9222412942985123 2 21 5.3006820543726825 2 22 6.365914685857945 2 23 5.560230774286584 2 24 6.395545073196438 2 25 6.159906236344906 2 26 4.674371119941743 2 27 4.321620274867037 2 28 4.2367832866961805 2 29 4.457545762167392 2 30 5.884281176856092 2 31 4.279692067277054 2 32 3.993494189814129 2 33 3.0719676866475183 2 34 4.832154619911076 2 35 3.938213979668646 2 36 5.585988894430063 2 37 5.9778822854621225 2 38 6.412445928368051 2 39 6.666358112580664 2 40 5.575022589302037 2 41 10.179966234046827 2 42 8.065550241151929 2 43 8.217099961655602 2 44 -3.1939585175279284 2 45 8.149822441672807 2 46 6.926779939012118 2 47 2.3369029077762633 2 48 7.042766195902086 2 49 0.1718573975741755 2 50 7.609461107284915 2 51 10.549791522864759 2 52 10.182304095330348 2 53 11.028037611822885 2 54 10.409532564660996 2 55 11.454513669622646 2 56 13.406273345207335 3 1 0.6601845786499894 3 2 1.3108885437635607 3 3 1.6439186646201023 3 4 1.8030703122283072 3 5 2.4816373391593274 3 6 2.9738526960695992 3 7 2.4246954744417906 3 8 2.3439117498027073 3 9 2.411345799285904 3 10 3.753428320066803 3 11 3.321592316282036 3 12 4.819932821500899 3 13 5.7937052803702045 3 14 5.826807925405376 3 15 1.372889636247983 3 16 6.74183321135002 3 17 7.039006154489435 3 18 8.736383222712227 3 19 5.754209635983088 3 20 6.85890796096518 3 21 7.3873487210393485 3 22 7.919248019191279 3 23 6.793564107619918 3 24 8.195545073196435 3 25 7.50657290301157 3 26 6.171037786608405 3 27 4.858286941533699 3 28 5.4584499533628446 3 29 5.09254576216739 3 30 5.355947843522756 3 31 4.9680254006103866 3 32 3.3751608564807967 3 33 4.180301019980853 3 34 5.352154619911079 3 35 6.074880646335309 3 36 6.777655561096726 3 37 6.047882285462123 3 38 6.252445928368054 3 39 5.651358112580663 3 40 10.168355922635367 3 41 10.296632900713494 3 42 9.16555024115193 3 43 10.068766628322269 3 44 10.786041482472069 3 45 9.08482244167281 3 46 11.956779939012119 3 47 10.541902907776262 3 48 11.162766195902083 3 49 13.363524064240842 3 50 12.604461107284912 3 51 10.85979152286476 3 52 11.717304095330345 3 53 11.068037611822884 3 54 12.167865897994325 3 55 11.302847002955978 3 56 9.431273345207334 4 1 1.0301845786499868 4 2 1.6058885437635624 4 3 1.813918664620104 4 4 1.8164036455616426 4 5 3.084970672492666 4 6 3.0538526960695975 4 7 2.046362141108453 4 8 2.170578416469368 4 9 2.5330124659525666 4 10 3.7334283200667997 4 11 5.831592316282034 4 12 5.121599488167561 4 13 6.202038613703543 4 14 5.925141258738712 4 15 6.121222969581318 4 16 7.338499878016684 4 17 8.615672821156096 4 18 8.376383222712228 4 19 7.574209635983088 4 20 7.463907960965177 4 21 7.0506820543726825 4 22 6.32424801919128 4 23 7.476897440953255 4 24 7.645545073196438 4 25 7.034906236344906 4 26 5.841037786608407 4 27 5.071620274867037 4 28 6.820116620029509 4 29 5.790879095500728 4 30 6.092614510189421 4 31 5.779692067277054 4 32 6.243494189814129 4 33 5.905301019980854 4 34 5.373821286577741 4 35 4.85488064633531 4 36 6.085988894430063 4 37 5.436215618795458 4 38 6.079112595034715 4 39 7.999691445913999 4 40 0.3666892559687014 4 41 10.804966234046827 4 42 10.190550241151929 4 43 10.133766628322267 4 44 10.139374815805407 4 45 10.024822441672807 4 46 13.010113272345453 4 47 13.461902907776263 4 48 12.126099529235422 4 49 12.796857397574179 4 50 12.151127773951579 4 51 10.799791522864759 4 52 9.223970761997016 4 53 10.653037611822885 4 54 10.20119923132766 4 55 10.579513669622646 4 56 9.572940011874003 5 1 0.2032615017269066 5 2 0.5770423899174091 5 3 0.6023802030816441 5 4 0.6753780045360003 5 5 1.5785604160824107 5 6 1.823083465300364 5 7 1.043157012903329 5 8 1.9269886728796237 5 9 1.4528842608243622 5 10 2.1565052431437195 5 11 3.196976931666647 5 12 4.3107020522701305 5 13 4.186012972677901 5 14 3.65911561771307 5 15 6.182120405478749 5 16 5.806448595965406 5 17 7.545160000643278 5 18 7.914844761173768 5 19 3.574209635983088 5 20 5.53121565327287 5 21 6.15965641334704 5 22 6.776171096114361 5 23 5.89356410761992 5 24 6.010929688581051 5 25 4.74964982608849 5 26 5.014114709685327 5 27 3.661363864610621 5 28 3.932296107208998 5 29 4.5633149929366255 5 30 4.675947843522756 5 31 1.180333092918083 5 32 1.38131470263464 5 33 4.318762558442394 5 34 3.255231542987996 5 35 3.5471883386430036 5 36 3.6853478687890338 5 37 3.3048053623852027 5 38 2.797061312983434 5 39 5.022127343349897 5 40 6.869894384173829 5 41 7.673555977636568 5 42 7.065550241151929 5 43 6.39338201293765 5 44 8.882964559395152 5 45 7.947899364749727 5 46 9.753703015935198 5 47 9.461902907776263 5 48 8.773535426671316 5 49 10.04044714116392 5 50 8.792153414977221 5 51 8.338253061326299 5 52 8.980381018407272 5 53 9.374191457976732 5 54 8.714019744148175 5 55 9.547462387571361 5 56 9.338965652899642 6 1 0.7801845786499868 6 2 1.2743668046331251 6 3 1.0856577950548854 6 4 1.5265485730978696 6 5 1.8277242956810724 6 6 2.4886353047652463 6 7 1.6351302570504842 6 8 1.5908682715418365 6 9 2.4043892775467697 6 10 3.0595152765885416 6 11 5.298983620629862 6 12 4.710367604109592 6 13 5.298053106457161 6 14 4.336373142796681 6 15 5.5433244188566775 6 16 6.552267993958715 6 17 7.025093111011174 6 18 8.028557135755705 6 19 6.074209635983088 6 20 5.860647091399958 6 21 4.423870460169784 6 22 7.3731610626695385 6 23 4.741390194576443 6 24 7.439023334066 6 25 6.217877250837656 6 26 5.7866899605214535 6 27 5.4774173763163105 6 28 5.561058649015017 6 29 4.4195022839065246 6 30 5.328121756566233 6 31 3.4880254006103897 6 32 4.18733476952427 6 33 1.4759531938939006 6 34 4.788676359041514 6 35 3.6592284724222637 6 36 4.073307735009774 6 37 4.988751850679513 6 38 4.368967667498488 6 39 4.030488547363273 6 40 4.538790705244065 6 41 9.487937248539577 6 42 9.174245893325843 6 43 9.030505758757048 6 44 8.219084960732943 6 45 7.003083311238026 6 46 7.850692982490379 6 47 11.37494638603713 6 48 11.303635761119477 6 49 10.224393629458234 6 50 7.788808933371872 6 51 8.038921957647368 6 52 8.339912790982524 6 53 7.114994133562014 6 54 8.150474593646504 6 55 7.09762961165163 6 56 8.944316823468206 7 1 -0.0531487546833489 7 2 0.6058885437635624 7 3 0.8972519979534397 7 4 0.3164036455616426 7 5 1.2099706724926662 7 6 2.0121860294029332 7 7 1.7546954744417889 7 8 1.5872450831360396 7 9 1.4913457992859023 7 10 3.1084283200667997 7 11 4.623258982948698 7 12 5.288266154834233 7 13 5.368705280370207 7 14 2.6751412587387122 7 15 6.704556302914646 7 16 7.04683321135002 7 17 7.407339487822767 7 18 8.084716556045564 7 19 7.074209635983088 7 20 7.922241294298512 7 21 7.842348721039347 7 22 8.82424801919128 7 23 7.351897440953255 7 24 9.020545073196438 7 25 7.618239569678234 7 26 5.924371119941743 7 27 6.613286941533701 7 28 5.403449953362845 7 29 5.332545762167392 7 30 7.342614510189421 7 31 6.279692067277054 7 32 5.660160856480793 7 33 5.571967686647518 7 34 4.915487953244412 7 35 6.313213979668646 7 36 5.877655561096727 7 37 7.936215618795458 7 38 8.454112595034715 7 39 8.958024779247328 7 40 10.241689255968705 7 41 12.221632900713491 7 42 13.190550241151929 7 43 12.258766628322267 7 44 12.514374815805407 7 45 12.899822441672807 7 46 13.551779939012118 7 47 14.420236241109599 7 48 14.20943286256875 7 49 14.463524064240843 7 50 14.81779444061825 7 51 13.966458189531423 7 52 13.182304095330348 7 53 14.403037611822885 7 54 11.867865897994328 7 55 14.871180336289314 7 56 14.114606678540671 8 1 0.9230417215071327 8 2 1.2785075913826134 8 3 1.563918664620104 8 4 1.6259274550854528 8 5 1.8111611486831407 8 6 2.625281267498167 8 7 0.9332669030132195 8 8 1.2658165117074631 8 9 2.586583894523997 8 10 3.757237843876325 8 11 5.22444945913918 8 12 5.1037423453104225 8 13 6.279419566084492 8 14 4.704903163500617 8 15 5.889080112438457 8 16 6.42183321135002 8 17 7.294244249727527 8 18 8.566859413188418 8 19 7.074209635983088 8 20 6.719860341917556 8 21 5.8006820543726825 8 22 6.723057543000806 8 23 6.679278393334204 8 24 7.443164120815489 8 25 7.3027633792020445 8 26 5.662466358036976 8 27 3.071620274867037 8 28 5.49273566764856 8 29 5.570641000262633 8 30 6.199757367332282 8 31 5.154692067277054 8 32 2.678017999337939 8 33 3.3993486390284744 8 34 5.832154619911076 8 35 5.5453568368115 8 36 6.425274608715778 8 37 4.918358475938312 8 38 7.460064975987102 8 39 6.719929541152094 8 40 3.075022589302037 8 41 9.85853766261825 8 42 9.089359764961454 8 43 9.223052342607982 8 44 9.686993863424451 8 45 8.858155775006143 8 46 12.248208510440687 8 47 10.985712431585789 8 48 10.70943286256875 8 49 10.773047873764654 8 50 11.484461107284915 8 51 11.156934380007613 8 52 9.223970761997016 8 53 10.50422808801336 8 54 12.225008755137182 8 55 9.758085098194073 8 56 7.8407971547311455 9 1 0.8635179119833225 9 2 1.1058885437635624 9 3 1.5222519979534397 9 4 1.2747369788949712 9 5 2.668304005825995 9 6 2.845519362736262 9 7 2.171362141108453 9 8 1.8372450831360396 9 9 2.4080124659525666 9 10 3.400094986733464 9 11 5.706592316282034 9 12 4.829932821500897 9 13 4.702038613703543 9 14 5.5918079254053765 9 15 5.204556302914646 9 16 7.255166544683355 9 17 7.282339487822767 9 18 9.209716556045564 9 19 8.365876302649752 9 20 7.255574627631841 9 21 7.384015387706011 9 22 6.32424801919128 9 23 7.64356410761992 9 24 8.520545073196438 9 25 5.57657290301157 9 26 6.674371119941743 9 27 5.946620274867037 9 28 4.153449953362845 9 29 6.624212428834056 9 30 6.425947843522756 9 31 5.446358733943725 9 32 4.910160856480793 9 33 5.946967686647518 9 34 4.040487953244412 9 35 6.35488064633531 9 36 6.377655561096727 9 37 6.519548952128787 9 38 6.829112595034715 9 39 6.583024779247328 9 40 1.825022589302037 9 41 7.513299567380159 9 42 8.523883574485264 9 43 6.800433294988935 9 44 9.014374815805407 9 45 6.941489108339475 9 46 7.801779939012118 9 47 10.545236241109599 9 48 10.126099529235422 9 49 9.546857397574176 9 50 10.067794440618247 9 51 9.174791522864759 9 52 9.473970761997016 9 53 8.278037611822885 9 54 5.95119923132766 9 55 7.37118033628931 9 56 4.864606678540667 10 1 1.0433424733868293 10 2 1.7045727542898774 10 3 1.7744449804095765 10 4 1.8514913648598892 10 5 2.0542689181067004 10 6 3.2117474329117 10 7 1.7678533691786313 10 8 1.8723328024342862 10 9 2.17555632560169 10 10 3.18079674111943 10 11 4.713171263650452 10 12 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0.14973697889497117 115 5 0.6016373391593319 115 6 0.38718602940293323 115 7 0.10469547444179028 115 8 0.2372450831360382 115 9 0.758012465952568 115 10 1.900094986733464 115 11 0.4149256496153697 115 12 -6.153400511832437 115 13 -6.472961386296458 115 14 -5.5331920745946235 115 15 -6.253777030418686 115 16 0.08849987801668746 115 17 0.1990061544894317 115 18 2.909716556045563 115 19 1.5408763026497532 115 20 2.6722412942985123 115 21 3.200682054372681 115 22 4.865914685857945 115 23 5.126897440953254 115 24 4.995545073196439 115 25 5.026572903011573 115 26 3.4910377866084055 115 27 2.3382869415337026 115 28 3.3117832866961763 115 29 3.7992124288340605 115 30 1.8759478435227592 115 31 1.7546920672770554 115 32 3.668494189814126 115 33 1.3803010199808554 115 34 2.165487953244412 115 35 4.35488064633531 115 36 5.177655561096724 115 37 5.59454895212879 115 38 6.079112595034715 115 39 6.624691445913999 115 40 8.40835592263537 115 41 6.563299567380156 115 42 6.832216907818594 115 43 4.708766628322266 115 44 -3.5606251841945955 115 45 3.5914891083394735 115 46 0.8767799390121169 115 47 -7.871430425557071 115 48 1.7760995292354202 115 49 -2.636475935759158 115 50 3.0177944406182498 115 51 3.699791522864757 115 52 6.023970761997013 115 53 6.0947042784895515 115 54 4.367865897994328 115 55 4.262847002955979 115 56 8.631273345207337 116 1 0.830184578649984 116 2 1.1808885437635652 116 3 1.8639186646201011 116 4 1.783070312228304 116 5 2.2016373391593334 116 6 3.2538526960696004 116 7 2.6546954744417874 116 8 2.1539117498027025 116 9 2.5913457992859037 116 10 3.783428320066797 116 11 4.181592316282035 116 12 4.679932821500898 116 13 4.94370528037021 116 14 4.2168079254053765 116 15 0.7128896362479793 116 16 5.321833211350018 116 17 6.949006154489432 116 18 7.126383222712228 116 19 6.374209635983085 116 20 7.138907960965174 116 21 7.317348721039348 116 22 8.149248019191283 116 23 6.14356410761992 116 24 7.645545073196438 116 25 7.4265729030115715 116 26 6.641037786608404 116 27 5.938286941533704 116 28 5.178449953362843 116 29 6.382545762167389 116 30 7.025947843522758 116 31 5.288025400610387 116 32 4.685160856480792 116 33 5.580301019980851 116 34 5.032154619911079 116 35 5.954880646335312 116 36 7.177655561096724 116 37 8.927882285462125 116 38 8.862445928368054 116 39 7.191358112580662 116 40 9.40835592263537 116 41 10.946632900713492 116 42 7.565550241151929 116 43 5.158766628322265 116 44 5.856041482472072 116 45 10.72482244167281 116 46 2.4267799390121176 116 47 -5.438097092223735 116 48 4.4427661959020845 116 49 -1.736475935759156 116 50 4.584461107284916 116 51 8.449791522864757 116 52 6.15730409533035 116 53 9.028037611822885 116 54 8.267865897994326 116 55 8.112847002955977 116 56 9.981273345207335 117 1 -0.35314875468334606 117 2 0.29755521043023236 117 3 0.630585331286774 117 4 0.5497369788949769 117 5 1.6683040058259948 117 6 2.1205193627362675 117 7 1.5713621411084588 117 8 0.37057841646937106 117 9 0.3580124659525694 117 10 2.5667616534001354 117 11 -0.918407683717966 117 12 -13.28673384516577 117 13 -10.406294719629791 117 14 -2.799858741261289 117 15 -8.787110363752017 117 16 -8.511500121983314 117 17 -1.2676605121772333 117 18 -5.356950110621106 117 19 0.007542969316421733 117 20 -1.1944253723681584 117 21 4.067348721039348 117 22 4.932581352524615 117 23 4.260230774286587 117 24 5.328878406529768 117 25 4.226572903011569 117 26 5.4910377866084055 117 27 5.271620274867033 117 28 4.911783286696178 117 29 5.065879095500726 117 30 6.142614510189425 117 31 4.221358733943724 117 32 4.001827523147462 117 33 4.580301019980851 117 34 4.6988212865777435 117 35 2.9548806463353117 117 36 7.310988894430057 117 37 6.994548952128788 117 38 5.812445928368049 117 39 5.824691445913995 117 40 7.741689255968705 117 41 5.363299567380157 117 42 4.565550241151929 117 43 -1.4912333716777333 117 44 -2.0272918508612605 117 45 2.7248224416728064 117 46 -4.056553394321217 117 47 -3.738097092223736 117 48 -2.7572338040979147 117 49 -6.96980926909249 117 50 -1.782205559381751 117 51 2.6331248561980907 117 52 0.9573040953303469 117 53 1.0280376118228851 117 54 2.70119923132766 117 55 0.9295136696226436 117 56 3.6312733452073367 118 1 -1.2198154213500132 118 2 -0.6782023653273441 118 3 -0.16335406265262264 118 4 -0.698747869589873 118 5 0.3652737027956974 118 6 0.8720345142514176 118 7 0.5956045653508824 118 8 0.23118447707543055 118 9 -1.0541087461686445 118 10 1.8697919564304328 118 11 0.3543195890093074 118 12 -8.510976269408193 118 13 -2.369931083266156 118 14 -7.896828438230987 118 15 -3.587110363752018 118 16 -14.396348606831797 118 17 -2.528266572783295 118 18 -12.805434959105954 118 19 -5.471244909471459 118 20 -2.20654658448937 118 21 -0.16901491532428992 118 22 2.1083389282821905 118 23 0.9390186530744664 118 24 0.9409996186509844 118 25 -0.4461543697157033 118 26 1.5455832411538601 118 27 2.1928323960791545 118 28 0.5057226806355715 118 29 1.1507275803492085 118 30 2.221402388977303 118 31 0.5789344915194832 118 32 -0.19211187079193337 118 33 0.780301019980854 118 34 0.5594273471838029 118 35 -0.09966480821014301 118 36 2.8322010156421804 118 37 3.2733368309166693 118 38 3.7760822920044177 118 39 3.1095399307624803 118 40 6.590174104453549 118 41 7.187541991622581 118 42 7.838277513879202 118 43 0.5996757192313567 118 44 -1.2394130629824751 118 45 4.7975497144000805 118 46 -1.7777655155333356 118 47 1.6437210895944467 118 48 1.951857104992996 118 49 -0.12738502666824658 118 50 1.4844611072849148 118 51 2.8452460683193053 118 52 5.466395004421258 118 53 5.391673975459248 118 54 5.822411352539781 118 55 4.299210639319615 118 56 9.304000617934609 119 1 -1.1531487546833432 119 2 -0.8357781229031076 119 3 -0.10274800204656032 119 4 -0.18359635443835742 119 5 0.8683040058259976 119 6 1.3871860294029332 119 7 0.4380288077751189 119 8 0.6372450831360368 119 9 0.29134579928589943 119 10 -0.23323834659986886 119 11 -0.45174101705129743 119 12 -8.820067178499102 119 13 -5.206294719629792 119 14 -9.533192074594623 119 15 -8.387110363752019 119 16 -11.378166788649981 119 17 -9.938455105675814E-4 119 18 -7.556950110621106 119 19 0.20754296931642102 119 20 -2.6610920390348234 119 21 4.000682054372678 119 22 3.799248019191282 119 23 2.926897440953251 119 24 2.128878406529772 119 25 4.693239569678237 119 26 6.157704453275077 119 27 2.938286941533704 119 28 4.311783286696176 119 29 3.132545762167389 119 30 5.075947843522755 119 31 3.288025400610387 119 32 3.868494189814129 119 33 2.0469676866475197 119 34 3.5654879532444106 119 35 3.0215473130019745 119 36 4.0443222277633915 119 37 4.327882285462124 119 38 4.7457792617013865 119 39 5.4246914459139965 119 40 9.075022589302034 119 41 0.8299662340468252 119 42 -0.3677830921814049 119 43 -7.5579000383444 119 44 -4.827291850861261 119 45 3.524822441672807 119 46 -2.256553394321216 119 47 -3.3380970922237374 119 48 -4.757233804097915 119 49 -4.236475935759156 119 50 -0.7155388927150845 119 51 5.499791522864758 119 52 3.690637428663681 119 53 4.56137094515622 119 54 5.434532564660994 119 55 2.9295136696226436 119 56 5.56460667854067 120 1 -0.9470881486227398 120 2 -0.49638418350916425 120 3 -0.16335406265262264 120 4 -0.24420241504441975 120 5 1.092546430068424 120 6 0.4174890597059644 120 7 0.5956045653508824 120 8 0.32209356798452404 120 9 0.7640730720131756 120 10 1.7788828655213464 120 11 -3.827498592808876 120 12 -14.96552172395365 120 13 -15.006294719629793 120 14 -7.896828438230987 120 15 -3.3143830910247445 120 16 -8.941803152286344 120 17 1.2899152453985216 120 18 -6.987253140924135 120 19 0.1651187268921781 120 20 -2.0247284026711867 120 21 4.285530539221167 120 22 6.290157110100374 120 23 3.848109562165373 120 24 3.486454164105531 120 25 4.553845630284297 120 26 5.454674150244774 120 27 4.011014214260975 120 28 5.687540862453751 120 29 5.150727580349212 120 30 5.76685693443185 120 31 4.124389036974023 120 32 5.353342674662613 120 33 5.234846474526307 120 34 3.6503364380928964 120 35 3.718517009971677 120 36 5.741291924733094 120 37 4.091518649098489 120 38 6.503355019277144 120 39 6.564085385307937 120 40 10.044719558999002 120 41 -6.812458008377419 120 42 -2.070813395211708 120 43 -0.9457788262231865 120 44 -13.87577669934611 120 45 -4.475177558327193 120 46 -12.1414018791697 120 47 -13.629006183132827 120 48 -9.411779258643369 120 49 -15.30920320848643 120 50 -7.606447983624177 120 51 -3.427481204407968 120 52 -3.1699686319423783 120 53 -2.6083260245407516 120 54 -1.5412250110965822 120 55 -1.7007893606803854 120 56 1.8494551633891518 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 38.0 5 9.0 6 94.0 7 47.0 8 101.0 9 189.0 10 70.0 11 84.0 12 87.0 13 38.0 14 56.0 15 51.0 16 68.0 17 76.0 18 89.0 19 127.0 20 189.0 21 246.0 22 291.0 23 367.0 24 435.0 25 458.0 26 552.0 27 563.0 28 605.0 29 661.0 30 666.0 31 791.0 32 853.0 33 898.0 34 1041.0 35 1166.0 36 1157.0 37 1287.0 38 1280.0 39 989.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.30020993701889 0.5216616833131879 0.1208728290603728 0.05725555060754501 8 99.370188943317 0.39442712640753225 0.12723455690565558 0.10814937336980723 9 98.3395890323812 0.7315987022075195 0.6616196959094091 0.26719256950187675 10 56.96927285450728 25.45963483682168 8.397480755773268 9.173611552897768 11 38.96558305235702 24.397226286659457 18.65894777021439 17.978242890769135 12 32.59749347922896 15.77708505630129 22.838602964565176 28.786818499904577 13 24.422673198040588 15.478083847573002 25.834976779693363 34.26426617469305 14 23.366626375723644 24.84254723582925 25.714103950632992 26.07672243781411 15 25.69511025886865 21.112176414189836 25.126238414829018 28.066474912112493 16 31.425843411839594 23.717377466581794 23.443666454487587 21.413112667091024 17 31.740071283095723 20.39205702647658 26.444755600814663 21.423116089613035 18 25.345123735606588 21.801641325784082 26.477511292066925 26.375723646542397 19 28.975175047740294 23.488224061107573 21.725015913430937 25.811584977721196 20 28.27748757803542 22.958338641865204 24.232386291247295 24.531787488852082 21 27.501749475157457 21.343596920923723 26.54112857051975 24.61352503339907 22 24.346332463897195 20.910999427444494 27.457217380240472 27.285450728417835 23 23.01036961638781 24.441758381576438 26.000381703670715 26.547490298365034 24 22.717730135504805 23.53839302754628 25.892232330300907 27.851644506648004 25 25.07475981421391 25.87643952408221 25.221098173951773 23.827702487752113 26 28.682367918523234 25.837046467218332 21.73138128580522 23.749204328453214 27 24.10873440285205 24.159663865546218 22.300738477209066 29.430863254392666 28 25.351485463451873 24.65805712831605 24.250906546217955 25.73955086201412 29 29.91970430792761 23.49604894213612 20.813153199082336 25.77109355085394 30 28.740608684579144 23.023048516490512 24.964981535718834 23.271361263211514 31 28.452335497009035 25.143184421534936 23.736795214458446 22.667684866997583 32 31.946035382461503 23.14496627211404 23.24678630520555 21.662212040218915 33 27.715833067856003 23.446957629818414 25.129021981522776 23.708187320802804 34 25.020712510356258 25.66439360142757 27.397871391243388 21.917022496972788 35 24.23547400611621 25.68170234454638 28.682466870540264 21.400356778797146 36 27.82763668767106 25.918146521545417 24.199605372032334 22.054611418751193 37 27.037532657872937 24.38666921557382 26.929204103740524 21.646594022812717 38 28.422059292317503 26.33726490277335 22.32068855594517 22.919987248963977 39 26.10661741290364 24.57805235335329 23.202343799757976 26.112986433985096 40 22.491016427104725 19.898613963039015 23.13911704312115 34.47125256673511 41 20.945641685429397 15.876487616596977 20.720488903184304 42.45738179478932 42 25.231259968102073 25.314194577352474 24.191387559808614 25.263157894736842 43 21.136552076025257 21.914662924931434 27.208367880604627 29.74041711843868 44 23.069060244042674 23.465150450392898 24.557592793713663 28.908196511850765 45 24.802396736359 23.546659867414586 26.54258031616522 25.108363080061192 46 22.897255902999362 25.95405232929164 25.673261008296105 25.47543075941289 47 19.77242216966055 23.473758230518442 25.66643227002493 31.087387329796073 48 24.729471674092935 23.042647994907703 26.429026098026736 25.79885423297263 49 22.057135569442675 23.536538706797604 25.46869021808443 28.937635505675296 50 25.267584097859324 24.050713557594293 27.242609582059124 23.439092762487256 51 23.233999106630083 24.7016782592049 27.99438453193798 24.06993810222704 52 22.592568988592188 25.033458670575488 28.455802689439807 23.918169651392517 53 24.57886676875957 23.991832567636546 26.29530372639102 25.133996937212867 54 23.89973210868733 23.6956244418931 27.554535017221582 24.850108432197985 55 22.303765156349716 25.341416719846844 27.28781110402042 25.067007019783027 56 23.29824561403509 23.987240829346092 28.29346092503987 24.421052631578945 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 0.5 9 0.0 10 0.0 11 0.0 12 1.0 13 8.5 14 15.0 15 20.5 16 26.0 17 27.5 18 29.0 19 35.5 20 42.0 21 30.5 22 26.0 23 33.0 24 28.5 25 24.0 26 32.5 27 41.0 28 53.0 29 71.5 30 78.0 31 83.0 32 88.0 33 105.0 34 122.0 35 148.5 36 175.0 37 199.0 38 225.5 39 228.0 40 244.5 41 261.0 42 303.0 43 345.0 44 362.0 45 379.0 46 521.0 47 799.5 48 936.0 49 937.5 50 939.0 51 866.0 52 793.0 53 872.0 54 967.0 55 983.0 56 1062.0 57 1141.0 58 1065.5 59 990.0 60 1046.5 61 1103.0 62 1112.0 63 1028.5 64 936.0 65 857.0 66 778.0 67 738.0 68 698.0 69 569.5 70 441.0 71 407.5 72 305.0 73 236.0 74 216.0 75 196.0 76 145.0 77 94.0 78 69.5 79 48.0 80 51.0 81 43.5 82 36.0 83 24.0 84 12.0 85 9.0 86 6.0 87 3.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.4707678605509256 16 0.05725555060754501 17 0.04453209491697945 18 0.0 19 0.05725555060754501 20 0.13359628475093838 21 0.0 22 0.0 23 0.0 24 0.0 25 0.012723455690565556 26 0.05725555060754501 27 0.06997900629811056 28 0.0 29 0.17176665182263504 30 0.08270246198867613 31 0.031808639226413894 32 0.031808639226413894 33 0.1526814682867867 34 0.1781283796679178 35 0.14631974044150392 36 0.050893822762262224 37 0.16540492397735226 38 0.21629874673961447 39 0.11451110121509002 40 0.8588332591131752 41 1.1069406450792034 42 0.27991602519244224 43 0.25446911381131115 44 0.4198740377886634 45 0.19721356320376612 46 0.31172466441885616 47 0.4834913162414912 48 0.05725555060754501 49 0.2353839302754628 50 0.14631974044150392 51 0.3053629365735734 52 0.1781283796679178 53 0.2990012087282906 54 0.2608308416565939 55 0.31172466441885616 56 0.27991602519244224 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 15719.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.942680832114 #Duplication Level Percentage of deduplicated Percentage of total 1 89.77355145458941 70.86964819645016 2 6.463051011362721 10.204211463833577 3 1.547264082520751 3.6643552388828806 4 0.6849867031992908 2.162987467396145 5 0.4109920219195745 1.6222406005471086 6 0.24981867999032958 1.183281379222597 7 0.2014666774115561 1.1133023729244862 8 0.11282133935047144 0.7125135186716712 9 0.05641066967523572 0.400788854252815 >10 0.4754613586912725 6.711622876773332 >50 0.024176001289386736 1.3550480310452317 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 94 0.5980024174565812 No Hit TTCCGCGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 65 0.4135123099433806 No Hit TTCCGCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 54 0.34353330364527007 No Hit TTCCGCGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 50 0.31808639226413893 No Hit TTCCGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 46 0.29263948088300784 No Hit TTCCGCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 44 0.27991602519244224 No Hit TTCCGCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 41 0.2608308416565939 No Hit TTCCGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 36 0.22902220243018004 No Hit TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 31 0.19721356320376612 No Hit TTCCGCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCAT 27 0.17176665182263504 No Hit TTCCGCGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG 26 0.16540492397735226 No Hit TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 26 0.16540492397735226 No Hit TTCCGCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCCACGGGCGCTGACCCC 25 0.15904319613206946 No Hit TTCCGCGGGACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGG 22 0.13995801259622112 No Hit TTCCGCGGGATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGG 22 0.13995801259622112 No Hit TTCCGCGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 21 0.13359628475093838 No Hit TTCCGCGGGCTCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCG 21 0.13359628475093838 No Hit TTCCGCGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG 20 0.12723455690565558 No Hit TTCCGCGGGCACACATCTAGATATCTTACTTTTACTCACTCCTCAGAAGCTCACCA 19 0.1208728290603728 No Hit TTCCGCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAG 19 0.1208728290603728 No Hit TTCCGCGGGACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGG 19 0.1208728290603728 No Hit TTCCGCGGGATCAGATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGG 19 0.1208728290603728 No Hit TTCCGCGGGAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGA 18 0.11451110121509002 No Hit TTCCGCGGGGTCAGCGTGTCCCACTATTCATGTCCCATCTCTGTGCATCCTGACTG 18 0.11451110121509002 No Hit TTCCGCGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 18 0.11451110121509002 No Hit TTCCGCGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC 18 0.11451110121509002 No Hit TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 17 0.10814937336980723 No Hit TTCCGCGGGGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGC 17 0.10814937336980723 No Hit TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 17 0.10814937336980723 No Hit TTCCGCGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT 16 0.10178764552452445 No Hit TTCCGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 16 0.10178764552452445 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.006361727845282779 0.0 0.0 0.0 0.0 11 0.012723455690565558 0.0 0.0 0.0 0.0 12 0.012723455690565558 0.0 0.0 0.0 0.0 13 0.012723455690565558 0.0 0.0 0.0 0.0 14 0.025446911381131115 0.0 0.0 0.0 0.0 15 0.031808639226413894 0.0 0.0 0.0 0.0 16 0.031808639226413894 0.0 0.0 0.0 0.0 17 0.031808639226413894 0.0 0.0 0.0 0.0 18 0.031808639226413894 0.0 0.0 0.0 0.0 19 0.03817036707169667 0.0 0.0 0.0 0.0 20 0.03817036707169667 0.0 0.0 0.0 0.0 21 0.03817036707169667 0.0 0.0 0.0 0.0 22 0.03817036707169667 0.0 0.0 0.0 0.0 23 0.05725555060754501 0.0 0.0 0.0 0.0 24 0.06361727845282779 0.0 0.0 0.0 0.0 25 0.06997900629811056 0.0 0.0 0.0 0.0 26 0.06997900629811056 0.0 0.0 0.0 0.0 27 0.06997900629811056 0.0 0.0 0.0 0.0 28 0.07634073414339335 0.0 0.0 0.0 0.0 29 0.07634073414339335 0.0 0.0 0.0 0.0 30 0.07634073414339335 0.0 0.0 0.0 0.0 31 0.08270246198867612 0.0 0.0 0.0 0.0 32 0.08270246198867612 0.0 0.0 0.0 0.0 33 0.0890641898339589 0.0 0.0 0.0 0.0 34 0.0890641898339589 0.0 0.0 0.0 0.0 35 0.0890641898339589 0.0 0.0 0.0 0.0 36 0.0890641898339589 0.0 0.0 0.0 0.0 37 0.0890641898339589 0.0 0.0 0.0 0.0 38 0.0890641898339589 0.0 0.0 0.0 0.0 39 0.0890641898339589 0.0 0.0 0.0 0.0 40 0.0890641898339589 0.0 0.0 0.0 0.0 41 0.09542591767924168 0.0 0.0 0.0 0.0 42 0.09542591767924168 0.0 0.0 0.0 0.0 43 0.10178764552452446 0.0 0.0 0.0 0.0 44 0.10814937336980723 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATTGA 15 0.007819747 50.72078 40 CGGATTG 15 0.007819747 50.72078 39 GCGGATT 15 0.007819747 50.72078 38 GCTCGTC 15 0.007819747 50.72078 38 CTCGTCT 15 0.007819747 50.72078 39 TGGGCGG 20 3.9025268E-4 50.39355 35 GATTCCT 15 0.008126692 50.23151 45 ATTCCTC 15 0.008126692 50.23151 46 AAAACTC 15 0.008230971 50.070515 50 CTCATTT 15 0.008230971 50.070515 50 TCTTCCA 15 0.008230971 50.070515 50 AAAAACT 20 4.0291745E-4 50.070515 49 CAAAAAC 15 0.008230971 50.070515 48 TATCTGA 15 0.008336243 49.91054 33 GCGGGGT 105 0.0 49.751595 5 TTGCATT 15 0.008442513 49.751595 19 GGATGGT 15 0.008442513 49.751595 8 TTGTTCT 15 0.008442513 49.751595 24 GGATGCA 15 0.008442513 49.751595 8 CGGGGTT 25 2.0536572E-5 49.751595 6 >>END_MODULE