FastQCFastQC Report
Fri 17 Jun 2016
SRR1526120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1151
Sequences flagged as poor quality0
Sequence length40
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT60.5212858384013901No Hit
TTCCGCGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.4344048653344918No Hit
TTCCGCGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.3475238922675934No Hit
TTCCGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT40.3475238922675934No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGACCC30.26064291920069504No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC20.1737619461337967No Hit
TTCCGCGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1737619461337967No Hit
TTCCGCGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT20.1737619461337967No Hit
TTCCGCGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT20.1737619461337967No Hit
TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTCGAGCC20.1737619461337967No Hit
TTCCGCGGGATCAACAAAAAAAAAAAAAAAAAAAAAAAAA20.1737619461337967No Hit
TTCCGCGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTT20.1737619461337967No Hit
TTCCGCGGGGATTTCTTTTTTTTTTTTTTTTTTTTTTTTT20.1737619461337967No Hit
TTCCGCGGGCTTTTTTTTTTTTTTTTTTTTTTTTTCCCTT20.1737619461337967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGCG1100.034.0000041
TCCGCGG1100.034.0000042
GCGGGGT200.001787722834.05
GCGGGGG200.001787722834.05
CGCGGGG502.910383E-1134.04
CGCGGGC200.001787722834.04
CCGCGGG1000.034.03
Produced by FastQC (version 0.11.5)