##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526120_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1151 Sequences flagged as poor quality 0 Sequence length 40 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.448305821025196 4.0 4.0 28.0 4.0 30.0 2 10.504778453518679 4.0 4.0 21.0 4.0 29.0 3 6.65855777584709 4.0 4.0 4.0 4.0 11.0 4 6.985230234578627 4.0 4.0 4.0 4.0 15.0 5 7.158123370981755 4.0 4.0 4.0 4.0 19.0 6 5.894005212858384 4.0 4.0 4.0 4.0 10.0 7 7.740225890529974 4.0 4.0 4.0 4.0 20.0 8 8.260642919200695 4.0 4.0 4.0 4.0 24.0 9 8.124239791485664 4.0 4.0 4.0 4.0 23.0 10 6.82015638575152 4.0 4.0 4.0 4.0 17.0 11 7.392701998262381 4.0 4.0 4.0 4.0 20.0 12 7.608166811468289 4.0 4.0 4.0 4.0 22.0 13 7.721112076455256 4.0 4.0 4.0 4.0 22.0 14 7.523023457862728 4.0 4.0 4.0 4.0 21.0 15 7.5960034752389225 4.0 4.0 4.0 4.0 22.0 16 8.261511728931364 4.0 4.0 4.0 4.0 25.0 17 8.054735013032145 4.0 4.0 4.0 4.0 24.0 18 7.795829713292789 4.0 4.0 4.0 4.0 23.0 19 7.763683753258037 4.0 4.0 4.0 4.0 22.0 20 7.999131190269331 4.0 4.0 4.0 4.0 24.0 21 7.784535186794092 4.0 4.0 4.0 4.0 24.0 22 7.783666377063423 4.0 4.0 4.0 4.0 25.0 23 7.715899218071242 4.0 4.0 4.0 4.0 24.0 24 7.526498696785404 4.0 4.0 4.0 4.0 23.0 25 7.364031277150304 4.0 4.0 4.0 4.0 22.0 26 7.429192006950478 4.0 4.0 4.0 4.0 24.0 27 7.345786272806255 4.0 4.0 4.0 4.0 24.0 28 7.3223284100781925 4.0 4.0 4.0 4.0 22.0 29 7.386620330147697 4.0 4.0 4.0 4.0 23.0 30 7.346655082536924 4.0 4.0 4.0 4.0 23.0 31 7.390095569070374 4.0 4.0 4.0 4.0 27.0 32 7.3266724587315375 4.0 4.0 4.0 4.0 27.0 33 7.280625543006082 4.0 4.0 4.0 4.0 24.0 34 7.262380538662033 4.0 4.0 4.0 4.0 25.0 35 7.187662901824501 4.0 4.0 4.0 4.0 23.0 36 6.534317984361425 4.0 4.0 4.0 4.0 4.0 37 6.33362293657689 4.0 4.0 4.0 4.0 4.0 38 6.336229365768896 4.0 4.0 4.0 4.0 4.0 39 6.4361424847958295 4.0 4.0 4.0 4.0 4.0 40 6.324066029539531 4.0 4.0 4.0 4.0 4.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 17.897435897435898 1 2 -6.023504273504274 1 3 -2.213675213675213 1 4 -2.5427350427350426 1 5 -2.264957264957265 1 6 -1.536324786324787 1 7 -3.412393162393162 1 8 -4.012820512820513 1 9 -4.083333333333334 1 10 -2.651709401709401 1 11 -3.497863247863247 1 12 -3.7863247863247853 1 13 -3.6709401709401703 1 14 -2.9423076923076925 1 15 -3.532051282051282 1 16 -4.337606837606838 1 17 -3.6880341880341874 1 18 -3.8931623931623944 1 19 -3.655982905982907 1 20 -4.100427350427351 1 21 -3.848290598290598 1 22 -3.9743589743589762 1 23 -3.878205128205127 1 24 -3.9038461538461524 1 25 -2.6260683760683765 1 26 -3.4893162393162402 1 27 -3.485042735042735 1 28 -3.3910256410256414 1 29 -3.1794871794871797 1 30 -3.2457264957264966 1 31 -2.769230769230769 1 32 -2.185897435897436 1 33 -2.0705128205128203 1 34 -2.0128205128205128 1 35 -2.064102564102564 1 36 -1.5705128205128203 1 37 -1.0769230769230766 1 38 -0.9423076923076925 1 39 -1.1089743589743586 1 40 -1.1538461538461542 2 1 -9.102564102564102 2 2 -6.023504273504274 2 3 -2.213675213675213 2 4 -2.5427350427350426 2 5 -2.264957264957265 2 6 -1.536324786324787 2 7 -3.412393162393162 2 8 -4.012820512820513 2 9 -4.083333333333334 2 10 -2.651709401709401 2 11 -3.497863247863247 2 12 -3.7863247863247853 2 13 -3.6709401709401703 2 14 -2.9423076923076925 2 15 -3.532051282051282 2 16 -4.337606837606838 2 17 -3.6880341880341874 2 18 -3.8931623931623944 2 19 -3.655982905982907 2 20 -4.100427350427351 2 21 -3.848290598290598 2 22 -3.9743589743589762 2 23 -3.878205128205127 2 24 -3.9038461538461524 2 25 -2.6260683760683765 2 26 -3.4893162393162402 2 27 -3.485042735042735 2 28 -3.3910256410256414 2 29 -3.1794871794871797 2 30 -3.2457264957264966 2 31 -2.769230769230769 2 32 -2.185897435897436 2 33 -2.0705128205128203 2 34 -2.0128205128205128 2 35 -2.064102564102564 2 36 -1.5705128205128203 2 37 -1.0769230769230766 2 38 -0.9423076923076925 2 39 -1.1089743589743586 2 40 -1.1538461538461542 4 1 -9.102564102564102 4 2 -6.023504273504274 4 3 -2.213675213675213 4 4 -2.5427350427350426 4 5 -2.264957264957265 4 6 -1.536324786324787 4 7 -3.412393162393162 4 8 -4.012820512820513 4 9 -4.083333333333334 4 10 -2.651709401709401 4 11 -3.497863247863247 4 12 -3.7863247863247853 4 13 -3.6709401709401703 4 14 -2.9423076923076925 4 15 -3.532051282051282 4 16 -4.337606837606838 4 17 -3.6880341880341874 4 18 -3.8931623931623944 4 19 -3.655982905982907 4 20 -4.100427350427351 4 21 -3.848290598290598 4 22 -3.9743589743589762 4 23 -3.878205128205127 4 24 -3.9038461538461524 4 25 -2.6260683760683765 4 26 -3.4893162393162402 4 27 -3.485042735042735 4 28 -3.3910256410256414 4 29 -3.1794871794871797 4 30 -3.2457264957264966 4 31 -2.769230769230769 4 32 -2.185897435897436 4 33 -2.0705128205128203 4 34 -2.0128205128205128 4 35 -2.064102564102564 4 36 -1.5705128205128203 4 37 -1.0769230769230766 4 38 -0.9423076923076925 4 39 -1.1089743589743586 4 40 -1.1538461538461542 5 1 -9.102564102564102 5 2 -6.023504273504274 5 3 -2.213675213675213 5 4 -2.5427350427350426 5 5 -2.264957264957265 5 6 -1.536324786324787 5 7 -3.412393162393162 5 8 -4.012820512820513 5 9 -4.083333333333334 5 10 -2.651709401709401 5 11 -3.497863247863247 5 12 -3.7863247863247853 5 13 -3.6709401709401703 5 14 -2.9423076923076925 5 15 -3.532051282051282 5 16 -4.337606837606838 5 17 -3.6880341880341874 5 18 -3.8931623931623944 5 19 -3.655982905982907 5 20 -4.100427350427351 5 21 -3.848290598290598 5 22 -3.9743589743589762 5 23 -3.878205128205127 5 24 -3.9038461538461524 5 25 -2.6260683760683765 5 26 -3.4893162393162402 5 27 -3.485042735042735 5 28 -3.3910256410256414 5 29 -3.1794871794871797 5 30 -3.2457264957264966 5 31 -2.769230769230769 5 32 -2.185897435897436 5 33 -2.0705128205128203 5 34 -2.0128205128205128 5 35 -2.064102564102564 5 36 -1.5705128205128203 5 37 -1.0769230769230766 5 38 -0.9423076923076925 5 39 -1.1089743589743586 5 40 -1.1538461538461542 6 1 19.897435897435898 6 2 17.976495726495727 6 3 4.786324786324787 6 4 10.457264957264957 6 5 3.735042735042735 6 6 4.463675213675213 6 7 14.587606837606838 6 8 8.987179487179487 6 9 20.916666666666664 6 10 15.3482905982906 6 11 1.502136752136753 6 12 22.213675213675216 6 13 22.32905982905983 6 14 6.0576923076923075 6 15 7.467948717948718 6 16 9.662393162393162 6 17 -0.6880341880341874 6 18 16.106837606837605 6 19 12.344017094017094 6 20 -2.100427350427351 6 21 -0.8482905982905979 6 22 15.025641025641024 6 23 19.121794871794872 6 24 13.096153846153847 6 25 16.373931623931625 6 26 27.51068376068376 6 27 27.514957264957264 6 28 13.608974358974358 6 29 19.82051282051282 6 30 23.754273504273502 6 31 23.23076923076923 6 32 -2.185897435897436 6 33 -2.0705128205128203 6 34 -2.0128205128205128 6 35 -2.064102564102564 6 36 -1.5705128205128203 6 37 -1.0769230769230766 6 38 -0.9423076923076925 6 39 -1.1089743589743586 6 40 -1.1538461538461542 9 1 11.897435897435898 9 2 19.976495726495727 9 3 1.286324786324787 9 4 0.9572649572649574 9 5 0.7350427350427351 9 6 1.463675213675213 9 7 4.087606837606838 9 8 5.487179487179487 9 9 7.416666666666666 9 10 6.348290598290599 9 11 2.502136752136753 9 12 4.213675213675215 9 13 4.32905982905983 9 14 4.0576923076923075 9 15 1.967948717948718 9 16 10.162393162393162 9 17 9.811965811965813 9 18 7.606837606837606 9 19 11.844017094017094 9 20 11.399572649572649 9 21 9.651709401709402 9 22 11.525641025641024 9 23 10.621794871794872 9 24 11.596153846153847 9 25 -2.6260683760683765 9 26 -3.4893162393162402 9 27 -3.485042735042735 9 28 -3.3910256410256414 9 29 -3.1794871794871797 9 30 -3.2457264957264966 9 31 -2.769230769230769 9 32 -2.185897435897436 9 33 -2.0705128205128203 9 34 -2.0128205128205128 9 35 -2.064102564102564 9 36 -1.5705128205128203 9 37 -1.0769230769230766 9 38 -0.9423076923076925 9 39 -1.1089743589743586 9 40 -1.1538461538461542 10 1 -9.102564102564102 10 2 -6.023504273504274 10 3 -2.213675213675213 10 4 -2.5427350427350426 10 5 -2.264957264957265 10 6 -1.536324786324787 10 7 -3.412393162393162 10 8 -4.012820512820513 10 9 -4.083333333333334 10 10 -2.651709401709401 10 11 -3.497863247863247 10 12 -3.7863247863247853 10 13 -3.6709401709401703 10 14 -2.9423076923076925 10 15 -3.532051282051282 10 16 -4.337606837606838 10 17 -3.6880341880341874 10 18 -3.8931623931623944 10 19 -3.655982905982907 10 20 -4.100427350427351 10 21 -3.848290598290598 10 22 -3.9743589743589762 10 23 -3.878205128205127 10 24 -3.9038461538461524 10 25 -2.6260683760683765 10 26 -3.4893162393162402 10 27 -3.485042735042735 10 28 -3.3910256410256414 10 29 -3.1794871794871797 10 30 -3.2457264957264966 10 31 -2.769230769230769 10 32 -2.185897435897436 10 33 -2.0705128205128203 10 34 -2.0128205128205128 10 35 -2.064102564102564 10 36 -1.5705128205128203 10 37 -1.0769230769230766 10 38 -0.9423076923076925 10 39 -1.1089743589743586 10 40 -1.1538461538461542 11 1 3.897435897435898 11 2 -6.023504273504274 11 3 -2.213675213675213 11 4 -2.5427350427350426 11 5 -2.264957264957265 11 6 -1.536324786324787 11 7 -3.412393162393162 11 8 -4.012820512820513 11 9 -4.083333333333334 11 10 -2.651709401709401 11 11 -3.497863247863247 11 12 -3.7863247863247853 11 13 -3.6709401709401703 11 14 -2.9423076923076925 11 15 -3.532051282051282 11 16 -4.337606837606838 11 17 -3.6880341880341874 11 18 -3.8931623931623944 11 19 -3.655982905982907 11 20 -4.100427350427351 11 21 -3.848290598290598 11 22 -3.9743589743589762 11 23 -3.878205128205127 11 24 -3.9038461538461524 11 25 -2.6260683760683765 11 26 -3.4893162393162402 11 27 -3.485042735042735 11 28 -3.3910256410256414 11 29 -3.1794871794871797 11 30 -3.2457264957264966 11 31 -2.769230769230769 11 32 -2.185897435897436 11 33 -2.0705128205128203 11 34 -2.0128205128205128 11 35 -2.064102564102564 11 36 -1.5705128205128203 11 37 -1.0769230769230766 11 38 -0.9423076923076925 11 39 -1.1089743589743586 11 40 -1.1538461538461542 12 1 -9.102564102564102 12 2 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17.897435897435898 14 2 -6.023504273504274 14 3 -2.213675213675213 14 4 -2.5427350427350426 14 5 -2.264957264957265 14 6 -1.536324786324787 14 7 -3.412393162393162 14 8 -4.012820512820513 14 9 -4.083333333333334 14 10 -2.651709401709401 14 11 -3.497863247863247 14 12 -3.7863247863247853 14 13 -3.6709401709401703 14 14 -2.9423076923076925 14 15 -3.532051282051282 14 16 -4.337606837606838 14 17 -3.6880341880341874 14 18 -3.8931623931623944 14 19 -3.655982905982907 14 20 -4.100427350427351 14 21 -3.848290598290598 14 22 -3.9743589743589762 14 23 -3.878205128205127 14 24 -3.9038461538461524 14 25 -2.6260683760683765 14 26 -3.4893162393162402 14 27 -3.485042735042735 14 28 -3.3910256410256414 14 29 -3.1794871794871797 14 30 -3.2457264957264966 14 31 -2.769230769230769 14 32 -2.185897435897436 14 33 -2.0705128205128203 14 34 -2.0128205128205128 14 35 -2.064102564102564 14 36 -1.5705128205128203 14 37 -1.0769230769230766 14 38 -0.9423076923076925 14 39 -1.1089743589743586 14 40 -1.1538461538461542 16 1 3.397435897435898 16 2 4.976495726495726 16 3 1.286324786324787 16 4 2.9572649572649574 16 5 0.2350427350427351 16 6 1.463675213675213 16 7 4.587606837606838 16 8 7.487179487179487 16 9 5.416666666666666 16 10 -1.1517094017094012 16 11 5.502136752136753 16 12 7.213675213675215 16 13 0.32905982905982967 16 14 3.0576923076923075 16 15 1.967948717948718 16 16 11.162393162393162 16 17 11.311965811965813 16 18 11.606837606837605 16 19 11.844017094017094 16 20 10.899572649572649 16 21 9.651709401709402 16 22 8.525641025641024 16 23 9.621794871794872 16 24 6.596153846153848 16 25 10.873931623931623 16 26 12.51068376068376 16 27 12.014957264957264 16 28 12.108974358974358 16 29 12.320512820512821 16 30 12.254273504273502 16 31 10.73076923076923 16 32 11.314102564102564 16 33 -2.0705128205128203 16 34 -2.0128205128205128 16 35 -2.064102564102564 16 36 -1.5705128205128203 16 37 -1.0769230769230766 16 38 -0.9423076923076925 16 39 -1.1089743589743586 16 40 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-3.2457264957264966 113 31 -2.769230769230769 113 32 -2.185897435897436 113 33 -2.0705128205128203 113 34 -2.0128205128205128 113 35 -2.064102564102564 113 36 -1.5705128205128203 113 37 -1.0769230769230766 113 38 -0.9423076923076925 113 39 -1.1089743589743586 113 40 -1.1538461538461542 116 1 -9.102564102564102 116 2 -6.023504273504274 116 3 -2.213675213675213 116 4 -2.5427350427350426 116 5 -2.264957264957265 116 6 -1.536324786324787 116 7 -3.412393162393162 116 8 -4.012820512820513 116 9 -4.083333333333334 116 10 -2.651709401709401 116 11 -3.497863247863247 116 12 -3.7863247863247853 116 13 -3.6709401709401703 116 14 -2.9423076923076925 116 15 -3.532051282051282 116 16 -4.337606837606838 116 17 -3.6880341880341874 116 18 -3.8931623931623944 116 19 -3.655982905982907 116 20 -4.100427350427351 116 21 -3.848290598290598 116 22 -3.9743589743589762 116 23 -3.878205128205127 116 24 -3.9038461538461524 116 25 -2.6260683760683765 116 26 -3.4893162393162402 116 27 -3.485042735042735 116 28 -3.3910256410256414 116 29 -3.1794871794871797 116 30 -3.2457264957264966 116 31 -2.769230769230769 116 32 -2.185897435897436 116 33 -2.0705128205128203 116 34 -2.0128205128205128 116 35 -2.064102564102564 116 36 -1.5705128205128203 116 37 -1.0769230769230766 116 38 -0.9423076923076925 116 39 -1.1089743589743586 116 40 -1.1538461538461542 118 1 -9.102564102564102 118 2 -6.023504273504274 118 3 -2.213675213675213 118 4 -2.5427350427350426 118 5 -2.264957264957265 118 6 -1.536324786324787 118 7 -3.412393162393162 118 8 -4.012820512820513 118 9 -4.083333333333334 118 10 -2.651709401709401 118 11 -3.497863247863247 118 12 -3.7863247863247853 118 13 -3.6709401709401703 118 14 -2.9423076923076925 118 15 -3.532051282051282 118 16 -4.337606837606838 118 17 -3.6880341880341874 118 18 -3.8931623931623944 118 19 -3.655982905982907 118 20 -4.100427350427351 118 21 -3.848290598290598 118 22 -3.9743589743589762 118 23 -3.878205128205127 118 24 -3.9038461538461524 118 25 -2.6260683760683765 118 26 -3.4893162393162402 118 27 -3.485042735042735 118 28 -3.3910256410256414 118 29 -3.1794871794871797 118 30 -3.2457264957264966 118 31 -2.769230769230769 118 32 -2.185897435897436 118 33 -2.0705128205128203 118 34 -2.0128205128205128 118 35 -2.064102564102564 118 36 -1.5705128205128203 118 37 -1.0769230769230766 118 38 -0.9423076923076925 118 39 -1.1089743589743586 118 40 -1.1538461538461542 120 1 -9.102564102564102 120 2 -6.023504273504274 120 3 -2.213675213675213 120 4 -2.5427350427350426 120 5 -2.264957264957265 120 6 -1.536324786324787 120 7 -3.412393162393162 120 8 -4.012820512820513 120 9 -4.083333333333334 120 10 -2.651709401709401 120 11 -3.497863247863247 120 12 -3.7863247863247853 120 13 -3.6709401709401703 120 14 -2.9423076923076925 120 15 -3.532051282051282 120 16 -4.337606837606838 120 17 -3.6880341880341874 120 18 -3.8931623931623944 120 19 -3.655982905982907 120 20 -4.100427350427351 120 21 -3.848290598290598 120 22 -3.9743589743589762 120 23 -3.878205128205127 120 24 -3.9038461538461524 120 25 -2.6260683760683765 120 26 -3.4893162393162402 120 27 -3.485042735042735 120 28 -3.3910256410256414 120 29 -3.1794871794871797 120 30 -3.2457264957264966 120 31 -2.769230769230769 120 32 -2.185897435897436 120 33 -2.0705128205128203 120 34 -2.0128205128205128 120 35 -2.064102564102564 120 36 -1.5705128205128203 120 37 -1.0769230769230766 120 38 -0.9423076923076925 120 39 -1.1089743589743586 120 40 -1.1538461538461542 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 812.0 5 53.0 6 19.0 7 7.0 8 3.0 9 6.0 10 4.0 11 10.0 12 16.0 13 22.0 14 19.0 15 13.0 16 8.0 17 11.0 18 8.0 19 9.0 20 24.0 21 17.0 22 21.0 23 23.0 24 14.0 25 10.0 26 10.0 27 5.0 28 4.0 29 2.0 30 0.0 31 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 96.41921397379912 0.7860262008733625 0.26200873362445415 2.532751091703057 8 98.00173761946134 0.3475238922675934 0.5212858384013901 1.1294526498696786 9 95.38729329852045 0.9573542210617928 1.5665796344647518 2.088772845953003 10 48.82710686359687 21.980886185925282 9.47002606429192 19.721980886185925 11 31.885317115551693 18.33188531711555 25.19548218940052 24.587315377932235 12 34.83927019982624 12.68462206776716 25.890529973935706 26.585577758470897 13 25.716768027801912 15.551694178974804 32.14596003475239 26.585577758470897 14 17.91304347826087 17.304347826086957 36.95652173913043 27.82608695652174 15 23.284100781928757 16.681146828844483 33.18853171155517 26.84622067767159 16 28.32319721980886 16.507384882710685 29.88705473501303 25.28236316246742 17 24.500434404865334 19.113814074717634 32.49348392701998 23.892267593397047 18 24.23979148566464 17.723718505647263 33.53605560382276 24.500434404865334 19 22.28024369016536 17.40644038294169 27.676240208877285 32.637075718015666 20 23.284100781928757 21.980886185925282 31.5377932232841 23.19721980886186 21 17.37619461337967 21.025195482189403 35.186794092093834 26.411815812337096 22 15.7254561251086 17.984361424847958 37.35881841876629 28.931364031277152 23 14.943527367506515 17.636837532580365 36.6637706342311 30.755864465682016 24 17.37619461337967 22.415291051259775 30.40834057341442 29.800173761946137 25 17.84160139251523 20.191470844212358 26.631853785900784 35.33507397737163 26 15.230635335073977 20.539599651871193 27.067014795474325 37.1627502175805 27 15.532286212914483 16.92844677137871 32.54799301919721 34.9912739965096 28 14.210985178727114 17.959895379250217 30.16564952048823 37.663469921534436 29 19.076655052264808 17.421602787456447 30.313588850174217 33.18815331010453 30 19.287576020851432 14.943527367506515 33.44917463075586 32.319721980886186 31 16.941789748045178 14.422241529105126 34.66550825369244 33.97046046915725 32 18.782608695652172 14.000000000000002 33.30434782608695 33.91304347826087 33 18.41876629018245 12.337098175499566 34.05734144222416 35.186794092093834 34 13.205907906168548 16.072980017376196 41.78974804517811 28.931364031277152 35 14.074717636837534 16.420503909643788 38.66203301476976 30.842745438748914 36 15.20417028670721 19.02693310165074 37.61946133796699 28.149435273675067 37 17.40644038294169 14.882506527415144 36.37946040034813 31.33159268929504 38 15.030408340573414 17.37619461337967 32.49348392701998 35.09991311902693 39 14.596003475238922 13.814074717636837 35.447437011294525 36.142484795829716 40 13.814074717636837 14.24847958297133 32.92788879235447 39.00955690703736 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 3.0 20 6.0 21 9.5 22 13.0 23 13.0 24 15.0 25 17.0 26 19.5 27 22.0 28 22.0 29 24.0 30 26.0 31 23.0 32 20.0 33 20.0 34 25.0 35 30.0 36 27.0 37 24.0 38 24.0 39 28.0 40 32.0 41 31.5 42 31.0 43 31.0 44 37.5 45 44.0 46 49.5 47 55.0 48 55.0 49 59.0 50 63.0 51 64.0 52 65.0 53 65.0 54 68.0 55 71.0 56 71.5 57 72.0 58 72.0 59 74.5 60 77.0 61 81.0 62 85.0 63 85.0 64 76.5 65 68.0 66 74.5 67 81.0 68 81.0 69 75.5 70 70.0 71 60.5 72 51.0 73 51.0 74 45.5 75 40.0 76 38.0 77 36.0 78 36.0 79 31.0 80 26.0 81 18.5 82 11.0 83 11.0 84 8.5 85 6.0 86 4.5 87 3.0 88 3.0 89 2.5 90 2.0 91 2.0 92 2.0 93 2.0 94 2.0 95 2.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5212858384013901 8 0.0 9 0.1737619461337967 10 0.0 11 0.0 12 0.0 13 0.0 14 0.08688097306689835 15 0.0 16 0.0 17 0.0 18 0.0 19 0.1737619461337967 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.1737619461337967 26 0.1737619461337967 27 0.4344048653344918 28 0.3475238922675934 29 0.26064291920069504 30 0.0 31 0.0 32 0.08688097306689835 33 0.0 34 0.0 35 0.0 36 0.0 37 0.1737619461337967 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1151.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.74109470026065 #Duplication Level Percentage of deduplicated Percentage of total 1 98.75555555555555 96.52476107732406 2 0.8 1.5638575152041705 3 0.08888888888888889 0.26064291920069504 4 0.17777777777777778 0.6950477845351868 5 0.08888888888888889 0.4344048653344918 6 0.08888888888888889 0.5212858384013901 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTCCGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.5212858384013901 No Hit TTCCGCGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.4344048653344918 No Hit TTCCGCGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.3475238922675934 No Hit TTCCGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT 4 0.3475238922675934 No Hit TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGACCC 3 0.26064291920069504 No Hit TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCC 2 0.1737619461337967 No Hit TTCCGCGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.1737619461337967 No Hit TTCCGCGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.1737619461337967 No Hit TTCCGCGGGGAGTCGCCTTTTTTTTTTTTTTTTTTTTTTT 2 0.1737619461337967 No Hit TTCCGCGGGGGGGTTGGGGATTTAGCTCAGTGGTCGAGCC 2 0.1737619461337967 No Hit TTCCGCGGGATCAACAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.1737619461337967 No Hit TTCCGCGGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTT 2 0.1737619461337967 No Hit TTCCGCGGGGATTTCTTTTTTTTTTTTTTTTTTTTTTTTT 2 0.1737619461337967 No Hit TTCCGCGGGCTTTTTTTTTTTTTTTTTTTTTTTTTCCCTT 2 0.1737619461337967 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.0 0.0 0.0 0.0 0.0 19 0.0 0.0 0.0 0.0 0.0 20 0.0 0.0 0.0 0.0 0.0 21 0.0 0.0 0.0 0.0 0.0 22 0.0 0.0 0.0 0.0 0.0 23 0.0 0.0 0.0 0.0 0.0 24 0.0 0.0 0.0 0.0 0.0 25 0.0 0.0 0.0 0.0 0.0 26 0.0 0.0 0.0 0.0 0.0 27 0.0 0.0 0.0 0.0 0.0 28 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCCGCG 110 0.0 34.000004 1 TCCGCGG 110 0.0 34.000004 2 GCGGGGT 20 0.0017877228 34.0 5 GCGGGGG 20 0.0017877228 34.0 5 CGCGGGG 50 2.910383E-11 34.0 4 CGCGGGC 20 0.0017877228 34.0 4 CCGCGGG 100 0.0 34.0 3 >>END_MODULE