FastQCFastQC Report
Fri 17 Jun 2016
SRR1526119_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526119_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1308
Sequences flagged as poor quality0
Sequence length40
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG70.5351681957186544No Hit
TTCCGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT60.45871559633027525No Hit
TTCCGCGGGGGGCTAGAGAGATGGCTCAGCGGTTAAGAGC40.3058103975535168No Hit
TTCCGCGGGAGTGAAACTGCGAATGGCTCATTAAATCAGT40.3058103975535168No Hit
TTCCGCGGGGTGCTGGATCTGGACGAGGACGAGGACGACC40.3058103975535168No Hit
TTCCGCGGGGGGTTCGATTCCGGAGAGGGAGCCTGAGAAA30.22935779816513763No Hit
TTCCGCGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG30.22935779816513763No Hit
TTCCGCGGGGCTGTCTGCCTTTAGTTTAACTTATTGCTAG30.22935779816513763No Hit
TTCCGCGGGTCACCGATCCTCTTCTGAGATGAGTTTTTGT30.22935779816513763No Hit
TTCCGCGGGGTTTCTGTTTGCCTCTCTCTGTCTCCGTCTC30.22935779816513763No Hit
TTCCGCGGGCCTTTCACATGTTCCCAGCTTTCCATTTCTA30.22935779816513763No Hit
TTCCGCGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACT30.22935779816513763No Hit
TTCCGCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGA30.22935779816513763No Hit
TTCCGCGGGATTTACAGTTGGTGGTCTAACCGGAATTGTT20.1529051987767584No Hit
TTCCGCGGGAAGCCATGCATGTCTAAGTACGCACGGCCGG20.1529051987767584No Hit
TTCCGCGGGGGAGTAAAGGTATGGTTAGTCCATTGCTTGT20.1529051987767584No Hit
TTCCGCGGGGGGGGAAAATCCGCGTTGAAGAACAGAGCAT20.1529051987767584No Hit
TTCCGCGGGGGAAGAGGTGCGGAAGAACAAAGAATCCAAA20.1529051987767584No Hit
TTCCGCGGGTCTTTTTCCTTTGTTATTTCCTTATCTTTTC20.1529051987767584No Hit
TTCCGCGGGTCACAGCTCCTCTTCTGAAGGAGATTGGGCG20.1529051987767584No Hit
TTCCGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1529051987767584No Hit
TTCCGCGGGGACTGGAAAACGTGATGAAGAAACTTGCAAA20.1529051987767584No Hit
TTCCGCGGGATCTGGAAATCATCAATACTGTGATCCAGAT20.1529051987767584No Hit
TTCCGCGGGGCTTTCCTTGAGGAGAACAGCCACGTGGCTG20.1529051987767584No Hit
TTCCGCGGGAGACTTGGCTGAGAGCATAATGAAGAACCTT20.1529051987767584No Hit
TTCCGCGGGCTCCATTAACTCCCTTGAATGAACCTGTGCA20.1529051987767584No Hit
TTCCGCGGGATCTTATTGACCCAGATATATTTTGATCAAC20.1529051987767584No Hit
TTCCGCGGGGGATATTGTCAGGCTATAGGGAAAACATGGG20.1529051987767584No Hit
TTCCGCGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCA20.1529051987767584No Hit
TTCCGCGGGGGGGATGGTGAGATGGCTCAGTGGGTAAGAG20.1529051987767584No Hit
TTCCGCGGGGGATTGATAAGTGGATGGTTTCTGTTTCTAA20.1529051987767584No Hit
TTCCGCGGGGGTTTACCAAACATTAAATATGTGACTCTGG20.1529051987767584No Hit
TTCCGCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTT20.1529051987767584No Hit
TTCCGCGGGGCTGGAGAGATGGCTCAGCAGTTAAAAGCAC20.1529051987767584No Hit
TTCCGCGGGACTAATCCTAGCCCTAGCCCTACACAAATAT20.1529051987767584No Hit
TTCCGCGGGGGATTTCTGAGTTTGAGGCCAGTCTGGTCTA20.1529051987767584No Hit
TTCCGCGGGCTATTCTGCTATTTAAAACCCTCATAAATTT20.1529051987767584No Hit
TTCCGCGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT20.1529051987767584No Hit
TTCCGCGGGGATGATTTTTGAAAATTAATGGTTCTATTGA20.1529051987767584No Hit
TTCCGCGGGGTGGACTGGAGAGATGGCTCAGCGGTTAAGA20.1529051987767584No Hit
TTCCGCGGGGAACTTGAAATGCCTGAATGTACTCCAAGTA20.1529051987767584No Hit
TTCCGCGGGGGCTACCACATCCAAGGAAGGCAGCTGGCGC20.1529051987767584No Hit
TTCCGCGGGGATTCTGGTCGTGTCATTCTTAAAGAAACTG20.1529051987767584No Hit
TTCCGCGGGGATCTTGGGAGCCAGTAGACTGGTTCTTCAC20.1529051987767584No Hit
TTCCGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1529051987767584No Hit
TTCCGCGGGCCCGTGTGTGTTTCTCATTTTGCACGTTTTT20.1529051987767584No Hit
TTCCGCGGGGGTTTTCGAAAGTGGTATTATAATGCTGCAG20.1529051987767584No Hit
TTCCGCGGGGCACTTTGAGACAACCTGTGATGACATTAAA20.1529051987767584No Hit
TTCCGCGGGGGGCTGGCGAGATGGTTCAGAGGTTAAGAGC20.1529051987767584No Hit
TTCCGCGGGGAATTGGGGTTGTATTGATGTCACTCTGGTT20.1529051987767584No Hit
TTCCGCGGGGACTAGGATGCCATGTGTAGAATTTTAAGTT20.1529051987767584No Hit
TTCCGCGGGATGCCACAACTAGATACATCAACATGATTTA20.1529051987767584No Hit
TTCCGCGGGGATGTTGGATCAGGACATCCCAATGGTGTAG20.1529051987767584No Hit
TTCCGCGGGGCTTCAGGCTGACGTTCTTCATCTGTTCAGT20.1529051987767584No Hit
TTCCGCGGGGGCTGGAGACAACACTTCATCAGTTTCGATA20.1529051987767584No Hit
TTCCGCGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT20.1529051987767584No Hit
TTCCGCGGGGTTCTCTTTGTGAAGAGCTTTCCCTCAGCCC20.1529051987767584No Hit
TTCCGCGGGGTCGCTGAGCCAAAGTTCTGCACTATGTAGC20.1529051987767584No Hit
TTCCGCGGGCGACGGAACGGTGAAGACTATCATGGTGGAT20.1529051987767584No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGC20.1529051987767584No Hit
TTCCGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCA20.1529051987767584No Hit
TTCCGCGGGTGCTGCATTGGCCAGTACATGCTTTTTTAAT20.1529051987767584No Hit
TTCCGCGGGGCTCCATTTGGCTGTTCCAGGCAAACCTGTA20.1529051987767584No Hit
TTCCGCGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCAC20.1529051987767584No Hit
TTCCGCGGGGGGGCTGAAGAGATGGCTCAGCAGTTAGGAG20.1529051987767584No Hit
TTCCGCGGGATCATTTTTTTATCTGTGATCCTGGGTGATG20.1529051987767584No Hit
TTCCGCGGGATGCCACAAATAGATACATCAACATGATTTA20.1529051987767584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGG306.3870084E-634.05
GCGGGGA251.1207712E-434.05
TTCCGCG1300.034.01
CGCGGGA306.3870084E-634.04
TCCGCGG1300.034.02
CCGCGGG1300.034.03
CGCGGGG700.033.9999964
CGCGGGC200.001888579233.9999964
Produced by FastQC (version 0.11.5)