FastQCFastQC Report
Fri 17 Jun 2016
SRR1526118_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526118_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70670
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG21893.0974954011603226No Hit
TATTGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT13941.9725484646950615No Hit
TATTGTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC12261.734823829064667No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG5290.748549596717136No Hit
TATTGTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC4040.571671147587378No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2660.3763973397481251No Hit
TATTGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT2600.3679071741898967No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG2270.3212112636196406No Hit
TATTGTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC2210.3127210980614122No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG1670.23630960803735673No Hit
TATTGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA1530.21649922173482386No Hit
TATTGTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC1400.19810386302532898No Hit
TATTGTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT1320.18678364228102448No Hit
TATTGTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC1200.1698033111645677No Hit
TATTGTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC1190.16838828357152966No Hit
TATTGTGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC1050.14857789726899676No Hit
TATTGTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC980.1386727041177303No Hit
TATTGTGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT920.13018253855950193No Hit
TATTGTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC920.13018253855950193No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC850.12027734540823547No Hit
TATTGTGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC810.11461723503608322No Hit
TATTGTGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG760.10754209707089289No Hit
TATTGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT750.10612706947785483No Hit
TATTGTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA710.10046695910570255No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCC150.00799481550.62608341
CGTCTCA700.050.62608341
TCAATTC150.00799481550.62608341
AATTATA150.00799481550.62608341
GATTGAC309.485757E-750.62608341
TGTTTAA203.965013E-450.48060238
CTCAATC203.965013E-450.48060240
CCGATTG309.676314E-750.48060239
CGATTGA251.953411E-550.48060240
GCCGATT354.816684E-850.48060238
TGTTCCC203.965013E-450.48060238
TGTTCCA309.676314E-750.48060238
GTTCCCT251.953411E-550.48060239
GCTCCAT150.00808637150.48060239
GCTCAAT203.965013E-450.48060239
ATGCTCG600.050.4081836
TCGGCCG150.00813244150.4081836
TTGTTCG150.00813244150.4081837
GATCGGC150.00820191150.2999334
ATTCGCC150.00822516550.2639544