##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526118_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 70670 Sequences flagged as poor quality 0 Sequence length 56 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.784802603650775 38.0 37.0 39.0 33.0 40.0 2 34.267496816187915 38.0 35.0 39.0 17.0 40.0 3 35.499660393377674 38.0 35.0 39.0 27.0 40.0 4 36.131427762841376 38.0 36.0 39.0 31.0 40.0 5 35.032871090986276 38.0 35.0 39.0 27.0 40.0 6 35.52742323475308 38.0 35.0 39.0 28.0 40.0 7 36.643625300693365 38.0 38.0 39.0 33.0 40.0 8 36.406381774444604 38.0 37.0 39.0 32.0 40.0 9 36.39042026319513 38.0 37.0 39.0 31.0 40.0 10 35.00686288382624 38.0 35.0 39.0 27.0 40.0 11 33.94934201216924 38.0 32.0 39.0 24.0 40.0 12 33.272774869109945 38.0 32.0 39.0 20.0 40.0 13 32.81906042167822 37.0 31.0 39.0 18.0 40.0 14 33.364879015140794 38.0 32.0 39.0 19.0 40.0 15 32.06435545493137 36.0 30.0 39.0 15.0 40.0 16 32.285439366067635 36.0 29.0 39.0 17.0 40.0 17 31.45582283854535 36.0 27.0 39.0 15.0 40.0 18 31.078590632517333 35.0 26.0 39.0 14.0 40.0 19 32.01542380076412 36.0 29.0 38.0 18.0 40.0 20 32.64999292486203 36.0 30.0 39.0 20.0 40.0 21 33.30166973255979 38.0 32.0 39.0 19.0 40.0 22 32.71579170793831 38.0 30.0 39.0 17.0 40.0 23 33.36879864157351 38.0 32.0 39.0 20.0 40.0 24 32.620616952030566 37.0 30.0 39.0 17.0 40.0 25 33.13383330974954 38.0 31.0 39.0 19.0 40.0 26 33.381519739634925 38.0 33.0 39.0 19.0 40.0 27 33.6851422102731 38.0 33.0 39.0 22.0 40.0 28 33.562742323475305 38.0 33.0 39.0 22.0 40.0 29 33.11076835998302 38.0 32.0 39.0 18.0 40.0 30 32.87268996745436 37.0 31.0 39.0 18.0 40.0 31 33.547332672987125 38.0 33.0 39.0 20.0 40.0 32 33.43705957266167 38.0 33.0 39.0 20.0 40.0 33 33.16458185934626 38.0 33.0 39.0 19.0 40.0 34 33.114603084760155 38.0 33.0 39.0 18.0 40.0 35 33.204344134710624 38.0 33.0 39.0 18.0 40.0 36 32.15321918777416 36.0 31.0 39.0 11.0 40.0 37 31.938191594736097 36.0 31.0 38.0 10.0 40.0 38 30.97293052214518 36.0 29.0 38.0 4.0 40.0 39 29.991679637752934 35.0 27.0 38.0 4.0 40.0 40 28.01228243950757 34.0 19.0 38.0 4.0 39.0 41 25.543922456487902 31.0 13.0 37.0 4.0 39.0 42 26.420602801754633 31.0 18.0 37.0 4.0 39.0 43 26.969308051507003 32.0 19.0 38.0 4.0 39.0 44 25.88069902363096 31.0 15.0 37.0 4.0 39.0 45 26.944686571388143 32.0 19.0 38.0 4.0 39.0 46 24.807386444035657 29.0 13.0 36.0 4.0 39.0 47 24.035064383755483 28.0 9.0 36.0 4.0 39.0 48 24.572307910004245 29.0 11.0 36.0 4.0 39.0 49 23.716145464836565 28.0 7.0 35.0 4.0 39.0 50 24.380925428045845 29.0 9.0 36.0 4.0 39.0 51 24.677826517617092 30.0 4.0 36.0 4.0 39.0 52 24.627380783925286 30.0 4.0 36.0 4.0 39.0 53 24.49885382764964 30.0 4.0 36.0 4.0 39.0 54 24.044474317249186 30.0 4.0 36.0 4.0 39.0 55 23.993519173623884 30.0 4.0 36.0 4.0 39.0 56 22.665911985283714 28.0 4.0 36.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.919781624200155 1 2 6.26283665321629 1 3 5.144931770935443 1 4 3.988992761292579 1 5 5.013548165581156 1 6 4.602165586907127 1 7 3.494998240449931 1 8 3.3772382717832343 1 9 3.6201980703010435 1 10 5.2338092727393715 1 11 -0.03666119709931337 1 12 -6.279104798284806 1 13 -18.386958958568346 1 14 -6.150986267817029 1 15 1.7342236085110585 1 16 -0.7844743706096544 1 17 -4.751003184092596 1 18 -3.85800168843209 1 19 0.7030598902884613 1 20 -0.04711933638729704 1 21 6.508407914845851 1 22 7.257154415246415 1 23 6.775194688186449 1 24 7.374322457134252 1 25 6.708213399356065 1 26 5.904296623825534 1 27 5.3061064291451245 1 28 6.239274604886074 1 29 6.275623203912264 1 30 6.613329937375639 1 31 5.271960386369198 1 32 4.700904055313281 1 33 5.050521151489349 1 34 5.299980516862366 1 35 5.482318640782211 1 36 4.545449002946434 1 37 4.533298650700846 1 38 5.536083005479107 1 39 5.6744337332864525 1 40 -10.580575635063214 1 41 0.5226640385452583 1 42 -1.272500350347638 1 43 -8.621761582124735 1 44 -14.137934654770794 1 45 -2.010468115883551 1 46 -4.350787753000635 1 47 -10.264185770772375 1 48 -4.40453354829517 1 49 -13.441121958571191 1 50 -3.1974889662959924 1 51 3.0717111516308897 1 52 2.22683101038162 1 53 2.761285150577617 1 54 3.000689103862964 1 55 2.2873029970055114 1 56 6.171901546669648 2 1 3.275401082623354 2 2 6.543849513668398 2 3 5.197412000985771 2 4 4.430082305185863 2 5 4.814330975709758 2 6 4.764079477792983 2 7 3.656752374166679 2 8 3.879434932858402 2 9 3.763739886740055 2 10 4.9675739504292835 2 11 3.9472832074058353 2 12 -0.611878981526278 2 13 1.0413500117966983 2 14 4.941513133093586 2 15 5.610252045287158 2 16 5.603096521634136 2 17 7.512116873419988 2 18 5.044386681245999 2 19 6.31532924087557 2 20 5.807661396898109 2 21 7.218129138585763 2 22 7.362993539777133 2 23 6.568628668536316 2 24 6.6812958575655905 2 25 6.829548969289771 2 26 5.519122089118287 2 27 5.276391595691969 2 28 5.6894703074182225 2 29 6.013861082337055 2 30 6.200677169582683 2 31 4.694677855815645 2 32 4.607685747141701 2 33 4.884693209960467 2 34 4.365241320440926 2 35 4.56499297579299 2 36 5.245904310878373 2 37 5.588654509795024 2 38 6.21616927435042 2 39 5.912711551003522 2 40 5.0751476655199 2 41 9.406600455191953 2 42 8.173381908618733 2 43 7.262254713106515 2 44 -3.4658362443546267 2 45 7.78472319332645 2 46 6.654244597826111 2 47 3.589796176667516 2 48 6.744679647646997 2 49 -0.5412897035987498 2 50 7.3582263464286655 2 51 9.149193378645826 2 52 8.001733159115545 2 53 8.557674638555888 2 54 8.291926423137667 2 55 8.028416504474158 2 56 8.323990371655672 3 1 3.3363851436074157 3 2 5.979515949334832 3 3 5.361653165226933 3 4 3.94613682124038 3 5 4.639463800842584 3 6 4.747909461622967 3 7 3.6983324157467266 3 8 3.48627253969601 3 9 3.7041418271419957 3 10 4.99182897468431 3 11 4.886299146421781 3 12 5.104914735267439 3 13 5.961192931639619 3 14 5.837563029143482 3 15 0.4242968593319709 3 16 6.510465429003041 3 17 7.677051038354147 3 18 8.23057286743219 3 19 7.168413093959423 3 20 6.293454882691595 3 21 7.122957043413674 3 22 8.166643343426937 3 23 6.852065951973604 3 24 7.678523854793593 3 25 6.878290018030821 3 26 6.36712393712013 3 27 5.747632066932439 3 28 5.679537297485215 3 29 6.132826201302176 3 30 6.66637363527915 3 31 5.527664688802471 3 32 5.412952552408505 3 33 5.468892794160055 3 34 4.9635319187315226 3 35 5.5712299820299975 3 36 6.878613943588007 3 37 7.371052292192811 3 38 7.869668927850078 3 39 9.328049966341933 3 40 10.625621215993451 3 41 13.803458852050351 3 42 12.71369144892827 3 43 12.414021864873668 3 44 12.59768881917044 3 45 12.144621553224813 3 46 13.204487148068662 3 47 13.517493104364448 3 48 12.725044628011975 3 49 13.541870379561335 3 50 12.276452264654587 3 51 12.14942437887683 3 52 11.948141105523494 3 53 12.07904215992341 3 54 11.094652225863467 3 55 11.243015719073373 3 56 11.012602060267358 4 1 3.381355734032553 4 2 6.4399433188531106 4 3 5.617628148474644 4 4 4.400980094265471 4 5 5.006673986234588 4 6 5.032921746635246 4 7 3.961901770225168 4 8 3.7479805286767274 4 9 4.050876992058981 4 10 5.060178315760922 4 11 6.397802385197746 4 12 6.660495109029629 4 13 6.7184708707357395 4 14 6.208942673250398 4 15 6.602764583254238 4 16 7.802997630626152 4 17 9.15229487723435 4 18 8.765231492999906 4 19 7.564958399595639 4 20 7.124889623217246 4 21 6.931980943346662 4 22 7.615306882999565 4 23 6.571595398775774 4 24 7.5039277711066 4 25 7.055938741134089 4 26 6.04734134461027 4 27 5.541913952123416 4 28 5.623919696413065 4 29 6.1973039021435135 4 30 6.71752395915675 4 31 4.601225853272727 4 32 4.817687866234728 4 33 4.7781028306428155 4 34 5.145350100549702 4 35 5.792583567019946 4 36 6.082098419799756 4 37 6.380135755821723 4 38 6.995571690116478 4 39 7.512697423716663 4 40 2.5401473123377265 4 41 12.816786229014088 4 42 10.840189848153944 4 43 10.67908031175029 4 44 11.897517573544643 4 45 11.065997474600731 4 46 12.849413065721851 4 47 13.567898882042947 4 48 13.065227786376955 4 49 12.91749393700307 4 50 13.105206638863507 4 51 12.308831720102347 4 52 11.885748134039613 4 53 11.7401991847168 4 54 12.261058301360453 4 55 12.087256654223399 4 56 10.817456501485438 5 1 2.941398852968952 5 2 6.3680784248538345 5 3 5.13721133208945 5 4 4.062783903104851 5 5 5.004446174520609 5 6 4.492210284184658 5 7 3.3396955179793935 5 8 3.222504423753982 5 9 3.5387677574201035 5 10 5.053638610406985 5 11 5.64940137908923 5 12 5.940480736050834 5 13 6.4503429512244175 5 14 6.209517592402392 5 15 7.0228867535740385 5 16 7.654452894729637 5 17 8.240544967065471 5 18 8.747768323758077 5 19 7.81008955302719 5 20 7.166858721312824 5 21 6.886059276081124 5 22 7.978080867907938 5 23 6.662720084366864 5 24 7.248519937833148 5 25 7.193056958884714 5 26 5.754276306881199 5 27 5.767138529917169 5 28 5.141346933207888 5 29 6.5477889901780095 5 30 6.359205597676329 5 31 5.122102604979521 5 32 5.13633680622754 5 33 4.492942931253673 5 34 4.928749310035872 5 35 5.132217056060547 5 36 6.3016456709675595 5 37 6.054875245580977 5 38 6.484396699099591 5 39 8.284023331010953 5 40 9.469647851324435 5 41 12.212546200268132 5 42 11.213887296950208 5 43 11.229924724254786 5 44 12.195181964489667 5 45 10.584574549699543 5 46 12.573308142976611 5 47 11.323055188187396 5 48 11.889086931184718 5 49 12.67387194634551 5 50 11.639162801278161 5 51 11.517383642488262 5 52 12.30622962882941 5 53 11.209476942532106 5 54 10.208165739376984 5 55 11.67137451264956 5 56 11.829601668571318 6 1 3.353256560478833 6 2 6.568868938687828 6 3 5.422586826160597 6 4 4.395194970298526 6 5 4.564525225904006 6 6 5.023830837544338 6 7 3.623885241299547 6 8 3.862030115453585 6 9 3.854182777182949 6 10 5.164310547165883 6 11 6.111851971974605 6 12 6.436528166880873 6 13 6.596983267429952 6 14 6.176711268291726 6 15 7.120119955155065 6 16 7.331923250460861 6 17 8.245683306986418 6 18 8.781760418619736 6 19 7.602974928521256 6 20 7.253815243051957 6 21 7.13032805078468 6 22 7.322744899528491 6 23 6.785644985552633 6 24 7.784093060362796 6 25 7.000566840307638 6 26 5.76139093138713 6 27 5.494806514106891 6 28 5.588382506330422 6 29 5.967551836027813 6 30 6.530747099652615 6 31 4.0813911425289255 6 32 4.801158940614897 6 33 4.50124332651059 6 34 4.818077373276974 6 35 5.22233563313565 6 36 5.731685196659257 6 37 6.217325838466355 6 38 7.074910533091682 6 39 7.77137510966708 6 40 5.555023345395579 6 41 12.264720113311615 6 42 10.353412988649811 6 43 10.403047253899054 6 44 12.134707656189274 6 45 10.593270201873455 6 46 12.426272569854081 6 47 13.327403014274353 6 48 12.759442662410013 6 49 12.914188151879106 6 50 12.122562010764334 6 51 11.914616844069293 6 52 11.456822514204898 6 53 11.50631488719614 6 54 10.953620284831526 6 55 11.231058307115962 6 56 11.433158980824281 7 1 2.931509679908423 7 2 5.827157708741304 7 3 5.011891638994818 7 4 3.60856395425575 7 5 4.255256063693672 7 6 4.220087522036316 7 7 3.230658859837874 7 8 3.250978422048952 7 9 3.2682647736178865 7 10 3.9698363938681993 7 11 5.330390296395283 7 12 5.911750983280154 7 13 6.171314818232094 7 14 5.629295938523455 7 15 6.413168083497315 7 16 6.738340362760326 7 17 8.313775998608524 7 18 8.648961844644692 7 19 6.739160311765467 7 20 6.60479563520882 7 21 6.018028585544034 7 22 6.681746682059686 7 23 5.88386245435834 7 24 6.683558301004506 7 25 5.584702312678406 7 26 5.223244763829197 7 27 5.215305622841292 7 28 4.892838834316162 7 29 5.214967165796079 7 30 4.967645495374541 7 31 4.304029288696487 7 32 3.9259361242156103 7 33 4.008373451287774 7 34 3.983139761868781 7 35 3.9496083604083765 7 36 5.579991792024678 7 37 5.349589653083111 7 38 6.508778625783307 7 39 7.44463714175264 7 40 6.861975217053331 7 41 10.888249525076315 7 42 10.765890707009884 7 43 9.81730750345342 7 44 10.917773609843461 7 45 10.47954471167738 7 46 11.974757418338928 7 47 11.89799124956847 7 48 11.823613785404664 7 49 11.998680130488733 7 50 11.273007643562906 7 51 10.693939482215463 7 52 10.924558699588143 7 53 10.628061767766546 7 54 11.078753974671098 7 55 11.234801622623987 7 56 9.496438838221785 8 1 3.499760056982325 8 2 6.7179948128137 8 3 5.687796616370385 8 4 4.540125040228595 8 5 5.0461685825473666 8 6 5.348481187194693 8 7 3.8394446818589927 8 8 4.008708437131908 8 9 3.8727142457144126 8 10 5.595779078634415 8 11 6.219719104841733 8 12 6.8774372577899605 8 13 6.937717533164221 8 14 6.723564415144871 8 15 7.80683324186835 8 16 8.23664353018114 8 17 8.819809181112294 8 18 9.75378839064771 8 19 7.895457446003775 8 20 7.4807383199750355 8 21 7.667915463372093 8 22 8.46662601840961 8 23 7.110295335202988 8 24 8.113988165257897 8 25 7.370147259888061 8 26 6.411216106212304 8 27 6.31805826235864 8 28 5.879641247589159 8 29 7.29377561225159 8 30 6.758369477274989 8 31 5.733139394277178 8 32 6.037172926628884 8 33 5.81418038944765 8 34 6.578916534116136 8 35 5.97845451425453 8 36 6.233083798057862 8 37 7.021346817487331 8 38 7.052707735888887 8 39 8.408438046730016 8 40 3.8240792894515216 8 41 12.920489344080845 8 42 11.15201438725121 8 43 10.84238291823472 8 44 12.160232131713752 8 45 10.899039432642692 8 46 12.163859982441497 8 47 12.667787629658967 8 48 12.095106998074346 8 49 12.125376963067918 8 50 12.560149423351746 8 51 11.622483976936422 8 52 11.329724612106997 8 53 11.492503698384951 8 54 10.896627277838522 8 55 11.375638726696383 8 56 11.164802337467638 9 1 3.549836218444625 9 2 6.744746526446598 9 3 5.728257393217298 9 4 4.589344385240018 9 5 5.427261499531369 9 6 5.139132367697357 9 7 3.937026555430961 9 8 3.95491940438248 9 9 3.942211580063237 9 10 5.454499566067767 9 11 6.243805167294138 9 12 6.676942208285009 9 13 7.053958964999712 9 14 6.838187415906482 9 15 7.715439487108259 9 16 7.783768434979315 9 17 8.20670940959668 9 18 9.382120454623337 9 19 7.52502713374178 9 20 7.198369698497498 9 21 7.42105712369197 9 22 7.846957320770617 9 23 6.782134634517529 9 24 7.798584509507709 9 25 6.813978181441751 9 26 6.443299122206209 9 27 5.934680501505632 9 28 6.419435611640953 9 29 6.530828163660576 9 30 6.327866811623814 9 31 5.172210224437119 9 32 5.551834008121645 9 33 4.920235225700509 9 34 5.1120467702166685 9 35 5.345647964368773 9 36 5.99127115525512 9 37 6.330467152597766 9 38 7.085171559194293 9 39 8.859313903546468 9 40 5.262674110472087 9 41 11.203333974697752 9 42 10.789866634014349 9 43 10.977664715645226 9 44 11.238488034227078 9 45 10.595250399893263 9 46 12.580367979395039 9 47 13.397700043977324 9 48 12.238290547198492 9 49 13.309687701834104 9 50 12.144974251988451 9 51 11.245579940378924 9 52 11.260512883241805 9 53 10.48498275398282 9 54 10.835888511654208 9 55 11.489024103695623 9 56 12.028568521778372 10 1 3.163221595443865 10 2 6.2179948128137 10 3 5.3608735394473115 10 4 4.2420481171516755 10 5 4.940399351778133 10 6 4.598481187194693 10 7 3.2240600664743724 10 8 3.8933238217472876 10 9 3.891945014945186 10 10 5.018856001711335 10 11 5.671642181764813 10 12 6.492821873174574 10 13 6.716563687010375 10 14 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4.5335946167764725 114 30 4.894116083609838 114 31 4.441284190657267 114 32 4.195543967352862 114 33 3.6015107061897282 114 34 4.360590742260936 114 35 5.0378436545260215 114 36 4.952540811632524 114 37 5.030962202102717 114 38 5.940151174802914 114 39 7.091695965282053 114 40 9.009034040582744 114 41 8.255896583899847 114 42 6.888439726617726 114 43 7.047699660316166 114 44 -0.9743832529016352 114 45 6.0579760842264 114 46 0.5286789869663799 114 47 -0.2784793386668234 114 48 2.411849079522309 114 49 -6.810143398923033 114 50 1.8073213690530991 114 51 4.022370854764478 114 52 4.449068503509714 114 53 4.755512748158704 114 54 4.005224562906395 114 55 4.5142133873298675 114 56 6.368987857829627 115 1 2.1521710787963357 115 2 5.195463239908989 115 3 4.285816134166019 115 4 3.0870538576798054 115 5 4.151363760503735 115 6 3.1876028863910193 115 7 2.693491754189644 115 8 2.615625773526851 115 9 2.7668015017419734 115 10 3.8546768972337233 115 11 0.25459281322291005 115 12 -4.551667219821979 115 13 -2.4085798261928417 115 14 -4.259788053282225 115 15 -9.325198905089167 115 16 2.5829408895382002 115 17 4.052874623132961 115 18 4.734270595010511 115 19 2.9934769637994094 115 20 3.5941137505146408 115 21 5.341996978871517 115 22 5.980546799121438 115 23 4.949417034399314 115 24 6.065911242180977 115 25 5.890526134744544 115 26 5.132082925959718 115 27 4.827099594161162 115 28 5.034922533467462 115 29 4.268230262079374 115 30 4.988277628824932 115 31 3.6177547788925644 115 32 4.620697610899832 115 33 3.014811847541793 115 34 4.396367739627042 115 35 3.994384479811359 115 36 6.249731329630762 115 37 6.161559217028088 115 38 6.681152052766038 115 39 6.860791663262734 115 40 9.73467056384877 115 41 10.825914143162361 115 42 10.09059073627532 115 43 8.143617131782364 115 44 -0.1609504170807412 115 45 8.405210500380921 115 46 3.151374333761815 115 47 -8.90095519468087 115 48 0.6726041278102812 115 49 -2.3086069634533253 115 50 2.5327384015377348 115 51 5.03795470024297 115 52 4.590201075941671 115 53 3.6909824584308737 115 54 5.10070305280982 115 55 5.02101760155287 115 56 7.0498482616926665 116 1 2.9027515099737826 116 2 5.728464898283782 116 3 5.036728240302011 116 4 3.749740424843985 116 5 4.516040377419159 116 6 4.3632247769382815 116 7 2.8875216049359125 116 8 3.064050317473786 116 9 3.2774151004152685 116 10 3.874411557266889 116 11 3.675488335610968 116 12 3.338548368901069 116 13 4.124255994702683 116 14 3.997923389503846 116 15 -2.3495770145419037 116 16 4.438993957531572 116 17 5.160834822137932 116 18 5.133275570134888 116 19 4.999944625490954 116 20 4.587148576385285 116 21 4.7788128992695285 116 22 5.5843610611446515 116 23 5.1028167027243505 116 24 5.732577908847642 116 25 4.883395123135923 116 26 4.897754567750763 116 27 4.580665099965479 116 28 3.3843421022900166 116 29 4.179673048149024 116 30 4.940848109753624 116 31 3.6621992233370086 116 32 3.2324720719280293 116 33 3.7881120133792763 116 34 3.674643029842631 116 35 3.4496083604083765 116 36 4.396331661305723 116 37 4.192073313213829 116 38 5.081981240162392 116 39 5.773395311687278 116 40 7.224720315092551 116 41 7.898053446644944 116 42 5.788766524003346 116 43 2.221229072080874 116 44 0.35895008043169696 116 45 6.737714646317901 116 46 1.9192018627833747 116 47 -5.394819207947869 116 48 -0.06985026688291995 116 49 0.7869154246063772 116 50 1.9821579703602907 116 51 6.300475429927879 116 52 6.0820750394574254 116 53 5.679042159923412 116 54 6.519276850488094 116 55 6.580553256610912 116 56 9.240229687894985 117 1 2.004338811561084 117 2 5.21417918399807 117 3 4.262125065698832 117 4 2.9783118534154127 117 5 3.517627679006459 117 6 3.8251295388430435 117 7 2.5859343033486084 117 8 2.4703995238229908 117 9 2.8345579575581255 117 10 3.780760763616094 117 11 0.23421849434112474 117 12 -4.356689726337024 117 13 -6.483680513233828 117 14 -0.7703305787501229 117 15 -7.703545268510158 117 16 -7.183228264690651 117 17 0.7148030761061896 117 18 -0.7968831600238389 117 19 0.16026208580841583 117 20 0.5585771478138604 117 21 5.423257343713974 117 22 5.392297569081162 117 23 4.539324639232287 117 24 5.494482670752408 117 25 4.824664964405766 117 26 5.14061171060791 117 27 4.479077798378171 117 28 4.7541833721312905 117 29 4.884434952910926 117 30 5.267832236737753 117 31 4.76061192174971 117 32 3.9959641354200883 117 33 4.656365981633243 117 34 4.852420807620408 117 35 4.187703598503617 117 36 6.056649121623188 117 37 4.011120932261445 117 38 6.166108224289374 117 39 6.721014359306327 117 40 8.010434600806835 117 41 7.636148684740185 117 42 5.391941127177951 117 43 -2.8232153723635705 117 44 -0.23628801480639794 117 45 5.8408892494925055 117 46 -1.8903219467404355 117 47 -0.14402555715421883 117 48 -1.9158820129146648 117 49 -1.0638782261872741 117 50 -0.0495880613857409 117 51 4.168729398181846 117 52 4.739217896600284 117 53 4.713962794844047 117 54 4.592292723503967 117 55 3.4154738915315477 117 56 6.798959846625145 118 1 2.174077674848334 118 2 5.537783894699558 118 3 4.158950462524231 118 4 2.8400630054891423 118 5 4.092384463440666 118 6 3.5094613360780613 118 7 2.1617151533230086 118 8 2.6206811418465463 118 9 2.522217967798781 118 10 4.373694711388751 118 11 0.7529076904496783 118 12 -2.5338530647906836 118 13 -5.072518198845707 118 14 -6.5010013416789505 118 15 -11.342050132821473 118 16 -8.414411060389579 118 17 -0.6789501240986233 118 18 -5.464573892230703 118 19 -1.2624209659068946 118 20 -0.7010234666254647 118 21 3.570210748731892 118 22 4.785794752900923 118 23 4.174501290537972 118 24 4.90462091960033 118 25 3.9762266643545594 118 26 4.288077148395924 118 27 4.41328158742067 118 28 4.142048195479983 118 29 5.296877349224292 118 30 5.58529255419807 118 31 2.8596902627635323 118 32 4.27476597873806 118 33 4.071624558182144 118 34 3.4635319187315226 118 35 3.2721890055696647 118 36 4.556905138008233 118 37 4.5712847827478775 118 38 4.889866544821885 118 39 6.013897103801977 118 40 8.423645046275343 118 41 6.274397532666452 118 42 6.150773692462128 118 43 -0.13683544404815962 118 44 -0.39373809161131135 118 45 5.728754072841198 118 46 -0.8370705386503161 118 47 -1.0725969857256459 118 48 -1.655513349320195 118 49 -0.17114909152265412 118 50 1.9947027732276723 118 51 5.397608046415332 118 52 4.815766795729829 118 53 3.6758163534717987 118 54 5.5113915458285945 118 55 5.090589098904818 118 56 7.0900504764254535 119 1 2.2233864306086986 119 2 5.493544263363148 119 3 4.258950462524233 119 4 3.221168996272553 119 5 3.668421329800111 119 6 3.7394152531287546 119 7 2.5875216049359153 119 8 2.638653492076962 119 9 2.385351608351776 119 10 3.97599885885419 119 11 -0.6673688072461772 119 12 -6.829705599352895 119 13 -2.5471725767258917 119 14 -5.297314705734252 119 15 -8.61624368120857 119 16 -7.364180645643035 119 17 -0.2724985111953977 119 18 -5.214343477484157 119 19 -0.14767442212809456 119 20 -0.046184756948044026 119 21 5.083574804031432 119 22 6.29547217225576 119 23 5.058372258279906 119 24 4.680196956466691 119 25 5.227839567580364 119 26 4.983468853465052 119 27 5.415585734886108 119 28 5.2351357530836715 119 29 5.670149238625211 119 30 5.75672112562664 119 31 4.389183350321137 119 32 5.09596413542009 119 33 5.2389056641729255 119 34 5.349246204445809 119 35 5.163894074694092 119 36 6.012204677178737 119 37 6.766676487817005 119 38 6.86452092270207 119 39 7.747998486290452 119 40 10.305672696044926 119 41 8.92186297045447 119 42 8.201464936701761 119 43 -1.3073423564905546 119 44 -0.2743832529016359 119 45 8.80755591615917 119 46 3.32237646595798 119 47 -0.01545412858278894 119 48 -4.015882012914663 119 49 -0.17022743253647832 119 50 3.704380192582512 119 51 8.835396064848513 119 52 9.250329007711393 119 53 8.807613588494839 119 54 7.693880025091268 119 55 9.001188177245833 119 56 10.451340799006097 120 1 2.5411353483576207 120 2 5.7415962114151 120 3 4.568041371615138 120 4 3.5042858793894354 120 5 4.428161589540373 120 6 3.9571641708776752 120 7 2.9178246352389436 120 8 3.1832422366657056 120 9 3.048122171122344 120 10 3.7006741835295145 120 11 -0.39420863408600226 120 12 -4.796805166452465 120 13 -3.3242288537821665 120 14 -3.665712974132518 120 15 -0.722304287269175 120 16 -6.88019796166035 120 17 0.46083482213793303 120 18 -3.4303607935014746 120 19 -0.13338870784237855 120 20 -0.22497263573592363 120 21 3.024267444724071 120 22 4.04092671771031 120 23 3.3856449855526343 120 24 3.581062757332493 120 25 3.1248092645500627 120 26 4.118966688962889 120 27 3.391776211076589 120 28 4.115655233603146 120 29 3.873612442088419 120 30 4.145898614804132 120 31 3.9359365970743845 120 32 2.3314619709179283 120 33 3.50124332651059 120 34 2.978683433883038 120 35 2.9950629058629232 120 36 4.1922912572653175 120 37 3.977931899072413 120 38 3.8385468967280474 120 39 5.2592538975458645 120 40 5.891386981759215 120 41 -6.071643523052025 120 42 -4.458708223471401 120 43 -6.924225473373674 120 44 -14.822868101386483 120 45 -4.4340025253992685 120 46 -9.58584864226713 120 47 -7.96653637966504 120 48 -6.740557337589989 120 49 -15.534296696605743 120 50 -4.553195564993246 120 51 0.4176471470995935 120 52 -0.35529869791631086 120 53 0.6396482205294731 120 54 0.39604452725577133 120 55 0.7704522465099011 120 56 3.4634620111273087 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1716.0 5 58.0 6 106.0 7 357.0 8 344.0 9 285.0 10 228.0 11 166.0 12 169.0 13 176.0 14 168.0 15 255.0 16 354.0 17 380.0 18 469.0 19 622.0 20 806.0 21 1147.0 22 1376.0 23 1690.0 24 1998.0 25 2190.0 26 2426.0 27 2385.0 28 2517.0 29 2542.0 30 2975.0 31 3225.0 32 3878.0 33 4152.0 34 4486.0 35 4819.0 36 5167.0 37 5713.0 38 5815.0 39 5507.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.60096221876327 0.2971557945379935 0.0792415452101316 0.02264044148860903 8 99.20899957549172 0.5136550162728173 0.18395358709494836 0.09339182114051224 9 95.99688693929531 1.1207018536861468 2.173482382906467 0.7089288241120701 10 36.284137540682046 23.28144898825527 31.185793122965897 9.248620348096788 11 23.313994622895144 30.9480684873355 15.752087165699733 29.98584972406962 12 24.56204895995472 28.927409084477144 16.363379085892173 30.14716286967596 13 17.80529220319796 15.198811376821848 20.0 46.995896419980184 14 20.61978208575067 35.06862883826234 25.372859770765533 18.938729305221454 15 42.316001933081274 17.56545470051454 18.785001563521618 21.333541802882568 16 27.899633253564804 34.45009274861585 20.505232154741506 17.145041843077838 17 21.95001132246377 16.677989130434785 42.85694067028986 18.515058876811594 18 18.80005660110372 17.192585255412478 27.69633507853403 36.311023064949765 19 27.3516130402163 17.938083037243604 19.95243690103761 34.757867021502484 20 18.488395159690537 26.226372318417546 35.44789865956301 19.837333862328904 21 18.72957025003891 17.327258062233795 30.313150037498758 33.63002165022853 22 17.724635630394793 16.976086033677657 38.41233904061129 26.88693929531626 23 18.02745153530494 20.100466959105702 34.69930663647941 27.17277486910995 24 16.88388877096158 20.005660510861105 30.00778320243402 33.10266751574329 25 28.542722101151895 19.815752978801687 34.039000367928004 17.602524552118414 26 38.47133938304288 20.22877528773872 17.23317807947676 24.066707249741636 27 20.660068810262075 33.99028727576491 18.056322473771395 27.293321440201616 28 29.11118343781397 35.69700142923854 17.060296885392052 18.131518247555437 29 38.56193404849292 26.46846259583091 17.94889962730455 17.02070372837162 30 29.680177820565458 20.828791074993276 33.47585406254866 16.0151770418926 31 29.50566790733219 35.62644174296995 18.98076732568178 15.887123024016075 32 37.13307102216435 27.781866560987346 18.451892320321566 16.633170096526737 33 28.73515994673165 27.950301759555717 19.40044768084323 23.91409061286941 34 20.16981827460097 28.02710288322513 35.084909137300485 16.718169704873414 35 19.981863009040158 21.367642474565702 42.89114971519256 15.759344801201575 36 28.055944847892867 27.45431123568466 27.325490862247136 17.164253054175337 37 27.830522885078647 20.276321383023948 35.35638373246422 16.536771999433185 38 35.90845280129324 20.415195473688687 25.99225762538818 17.684094099629895 39 26.851838730248705 20.966484801247077 20.17572450931765 32.00595195918657 40 22.369040654118923 16.852409423650453 32.511879450334625 28.266670471896006 41 19.9965683400775 14.780445257874945 29.013254786450666 36.209731615596894 42 24.83895683759471 28.352109878259885 22.511989557024886 24.29694372712052 43 15.856686768357573 25.312522170982614 34.76410074494501 24.06669031571479 44 17.541316735587102 27.005442582882154 27.815435335578577 27.637805345952167 45 24.941500148908712 21.004637442741057 34.33409442230511 19.719767986045124 46 17.177496060532928 29.103788986527736 21.951704263142204 31.767010689797136 47 16.37465157289948 30.963080948859435 20.34956482166221 32.31270265657887 48 24.74831145660762 29.37853107344633 20.62642481910992 25.24673265083613 49 16.4708886618999 33.45675859720804 20.089944387697198 29.982408353194874 50 24.14413903576542 29.879647874346144 21.30190096820379 24.674312121684647 51 17.533822631705966 35.88393123323065 22.901435243679106 23.68081089138428 52 18.188519825911907 36.082167311699905 28.209926423680514 17.519386438707667 53 18.517940496289885 33.307322333044844 28.977200175928946 19.197536994736318 54 18.495297805642632 29.80893346004908 27.91670803841189 23.779060695896394 55 17.73412996891986 27.91819820331238 30.567815733079772 23.779856094687993 56 24.307033222685618 22.04584787357789 29.828070474082903 23.819048429653588 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 1.0 3 2.0 4 6.0 5 9.0 6 62.0 7 115.0 8 153.0 9 191.0 10 207.5 11 224.0 12 214.5 13 196.5 14 188.0 15 194.5 16 201.0 17 194.5 18 188.0 19 212.0 20 236.0 21 244.5 22 353.5 23 454.0 24 473.5 25 493.0 26 500.5 27 508.0 28 530.5 29 636.5 30 720.0 31 833.5 32 947.0 33 993.5 34 1040.0 35 1141.0 36 1242.0 37 1379.5 38 1759.0 39 2001.0 40 2844.5 41 3688.0 42 6344.5 43 9001.0 44 8750.0 45 8499.0 46 6961.5 47 4864.5 48 4305.0 49 4239.0 50 4173.0 51 3982.5 52 3792.0 53 3524.0 54 3245.5 55 3235.0 56 3249.0 57 3263.0 58 2945.0 59 2627.0 60 2378.5 61 2130.0 62 1935.5 63 1492.0 64 1243.0 65 1115.5 66 988.0 67 831.5 68 675.0 69 614.0 70 553.0 71 419.0 72 223.5 73 162.0 74 135.5 75 109.0 76 135.5 77 162.0 78 108.0 79 33.5 80 13.0 81 7.5 82 2.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.4471487194000283 16 0.06933635205886515 17 0.0198103863025329 18 0.0 19 0.03820574501202774 20 0.13584264893165418 21 0.0014150275930380643 22 0.0 23 0.0 24 0.007075137965190322 25 0.005660110372152257 26 0.04669591057025612 27 0.05801613131456064 28 0.004245082779114193 29 0.14574784208292063 30 0.052356020942408384 31 0.012735248337342577 32 0.02264044148860903 33 0.1188623178151974 34 0.17546342153671998 35 0.13584264893165418 36 0.041035800198103864 37 0.14150275930380643 38 0.21083911136267158 39 0.14857789726899676 40 0.8362813074854959 41 1.0372152256969012 42 0.27168529786330836 43 0.27593038064242253 44 0.4230932503183812 45 0.2221593321069761 46 0.32404131880571674 47 0.5009197679354748 48 0.06650629687278903 49 0.2575350219329277 50 0.17970850431583416 51 0.32404131880571674 52 0.1853686146879864 53 0.26461015989811804 54 0.241969718409509 55 0.2929107117588793 56 0.24904485637469934 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 70670.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.01004669591057 #Duplication Level Percentage of deduplicated Percentage of total 1 89.26851930061662 59.81887646809113 2 6.168172987583411 8.26659119852837 3 1.7484584846693134 3.514928541106552 4 0.774980995016471 2.0772605065798784 5 0.432891291494214 1.4504032828640159 6 0.33786637384914264 1.3584264893165416 7 0.23017146718472842 1.079666053488043 8 0.14781653855899993 0.7924154521013159 9 0.13514654953965705 0.8150558935899249 >10 0.684179407044514 8.208575067213811 >50 0.038009967058028546 1.7900099051931513 >100 0.0253399780386857 3.2743738502900808 >500 0.0021116648365571413 0.748549596717136 >1k 0.006334994509671425 6.804867694920051 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 2189 3.0974954011603226 No Hit TATTGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 1394 1.9725484646950615 No Hit TATTGTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 1226 1.734823829064667 No Hit TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 529 0.748549596717136 No Hit TATTGTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 404 0.571671147587378 No Hit TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 266 0.3763973397481251 No Hit TATTGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 260 0.3679071741898967 No Hit TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 227 0.3212112636196406 No Hit TATTGTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCTC 221 0.3127210980614122 No Hit TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 167 0.23630960803735673 No Hit TATTGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAAAAA 153 0.21649922173482386 No Hit TATTGTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCCGATTGAACAAAAACTC 140 0.19810386302532898 No Hit TATTGTGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 132 0.18678364228102448 No Hit TATTGTGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 120 0.1698033111645677 No Hit TATTGTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGACCAAAAACTC 119 0.16838828357152966 No Hit TATTGTGGGTCACAGATCCTCTGAAGGAGATTGGGCGGATTGAACAAAAACTCATC 105 0.14857789726899676 No Hit TATTGTGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 98 0.1386727041177303 No Hit TATTGTGGGGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGGCGCCGGCGGCTT 92 0.13018253855950193 No Hit TATTGTGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 92 0.13018253855950193 No Hit TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 85 0.12027734540823547 No Hit TATTGTGGGTCACAGATCCTCTCCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 81 0.11461723503608322 No Hit TATTGTGGGAGTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTG 76 0.10754209707089289 No Hit TATTGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGCTCAATCCGCCCAATCT 75 0.10612706947785483 No Hit TATTGTGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 71 0.10046695910570255 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0028300551860761286 0.0 0.0 0.0 0.0 11 0.015565303523418707 0.0 0.0 0.0 0.0 12 0.016980331116456773 0.0 0.0 0.0 0.0 13 0.028300551860761285 0.0 0.0 0.0 0.0 14 0.07782651761709353 0.0 0.0 0.0 0.0 15 0.08490165558228385 0.0 0.0 0.0 0.0 16 0.08914673836139805 0.0 0.0 0.0 0.0 17 0.10471204188481675 0.0 0.0 0.0 0.0 18 0.10754209707089288 0.0 0.0 0.0 0.0 19 0.10754209707089288 0.0 0.0 0.0 0.0 20 0.11037215225696902 0.0 0.0 0.0 0.0 21 0.11603226262912127 0.0 0.0 0.0 0.0 22 0.12169237300127353 0.0 0.0 0.0 0.0 23 0.12593745578038773 0.0 0.0 0.0 0.0 24 0.1301825385595019 0.0 0.0 0.0 0.0 25 0.1386727041177303 0.0 0.0 0.0 0.0 26 0.1499929248620348 0.0 0.0 0.0 0.0 27 0.16697325597849158 0.0 0.0 0.0 0.0 28 0.17687844912975803 0.0 0.0 0.0 0.0 29 0.17687844912975803 0.0 0.0 0.0 0.0 30 0.18112353190887223 0.0 0.0 0.0 0.0 31 0.19527380783925286 0.0 0.0 0.0 0.0 32 0.20093391821140513 0.0 0.0 0.0 0.0 33 0.20376397339748126 0.0 0.0 0.0 0.0 34 0.20376397339748126 0.0 0.0 0.0 0.0 35 0.20800905617659543 0.0 0.0 0.0 0.0 36 0.21508419414178576 0.0 0.0 0.0 0.0 37 0.21649922173482383 0.0 0.0 0.0 0.0 38 0.21791424932786188 0.0 0.0 0.0 0.0 39 0.22074430451393803 0.0 0.0 0.0 0.0 40 0.2249893872930522 0.0 0.0 0.0 0.0 41 0.22640441488609028 0.0 0.0 0.0 0.0 42 0.2334795528512806 0.0 0.0 0.0 0.0 43 0.2447997735955851 0.0 0.0 0.0 0.0 44 0.2617801047120419 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAATCC 15 0.007994815 50.626083 41 CGTCTCA 70 0.0 50.626083 41 TCAATTC 15 0.007994815 50.626083 41 AATTATA 15 0.007994815 50.626083 41 GATTGAC 30 9.485757E-7 50.626083 41 TGTTTAA 20 3.965013E-4 50.480602 38 CTCAATC 20 3.965013E-4 50.480602 40 CCGATTG 30 9.676314E-7 50.480602 39 CGATTGA 25 1.953411E-5 50.480602 40 GCCGATT 35 4.816684E-8 50.480602 38 TGTTCCC 20 3.965013E-4 50.480602 38 TGTTCCA 30 9.676314E-7 50.480602 38 GTTCCCT 25 1.953411E-5 50.480602 39 GCTCCAT 15 0.008086371 50.480602 39 GCTCAAT 20 3.965013E-4 50.480602 39 ATGCTCG 60 0.0 50.40818 36 TCGGCCG 15 0.008132441 50.40818 36 TTGTTCG 15 0.008132441 50.40818 37 GATCGGC 15 0.008201911 50.29993 34 ATTCGCC 15 0.008225165 50.26395 44 >>END_MODULE