FastQCFastQC Report
Fri 17 Jun 2016
SRR1526117_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526117_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10191
Sequences flagged as poor quality0
Sequence length40
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC220.21587675399862624No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG170.16681385536257481No Hit
TATTGTGGGCTCGTCTTAATCAGCAACATCTTTTGATACA150.14718869590815425No Hit
TATTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT140.13737611618094397No Hit
TATTGTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGC140.13737611618094397No Hit
TATTGTGGGCTCGTCTTAATCCGCAACATCTTTTGATACA130.1275635364537337No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG130.1275635364537337No Hit
TATTGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT120.11775095672652341No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGTT200.002714978333.8620726
TGGGGTC200.002714978333.8620726
TGGGGGC200.002714978333.8620726
TGGGGGA407.05877E-833.8620726
TGGGGAC200.002714978333.8620726
GTGGGCA407.05877E-833.8620725
TGGGCAG200.002714978333.8620726
TGGGACA200.002714978333.8620726
TTGTGGC200.002714978333.8620723
TGGGGCC251.9362103E-433.862076
GTGGGGC601.8189894E-1233.862075
GTGGGCG251.9362103E-433.862075
GTGGGAC455.0404196E-933.862075
GGGATGG251.9362103E-433.862077
TGGGTGG251.9362103E-433.862076
TGGGATG301.3825498E-533.862076
TGGGAGC301.3825498E-533.862076
TGGGAAC301.3825498E-533.862076
GTGGGGG1650.032.8359455
TGGGGGG900.031.9808436