FastQCFastQC Report
Fri 17 Jun 2016
SRR1526116_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526116_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048
Sequences flagged as poor quality0
Sequence length40
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT80.390625No Hit
TATTGTGGGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTT70.341796875No Hit
TATTGTGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.244140625No Hit
TATTGTGGGTACCTGGTTGATCCCCCCAGTACCATATGCT40.1953125No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG40.1953125No Hit
TATTGTGGGGCCTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.1953125No Hit
TATTGTGGTCGCCTTTTTTTTTTTTTTTTTTTTTTTTTTT40.1953125No Hit
TATTGTGGGGGGCTGGTGCGATGGCCCAGTGGGTAAGAGC30.146484375No Hit
TATTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT30.146484375No Hit
TATTGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT30.146484375No Hit
TATTGTGGGTACCTGGTTGATCCCCCCCGTACCATATGCT30.146484375No Hit
TATTGTGGGTGACGAGTCTTTTTTTTTTTTTTTTTTTTTT30.146484375No Hit
TATTGTGGGCGTCGCCTTTTTTTTTTTTTTTTTTTTTTTT30.146484375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGGGT200.002233637733.8255
GTGGGAG200.002233637733.8255
TGTGGGT251.4381566E-433.8254
TGTGGGC355.581787E-733.8249974
TATTGTG2150.031.4651171
ATTGTGG2100.031.4089282
TTGTGGG1950.030.355773
TGTGGGG701.8189894E-1228.9928554
GTGGGGG250.00667114427.0600015
TGTGGGA701.05501385E-1026.5767844
Produced by FastQC (version 0.11.5)