FastQCFastQC Report
Fri 17 Jun 2016
SRR1526115_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526115_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6178
Sequences flagged as poor quality0
Sequence length40
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT1372.217546131434121No Hit
TATTGTGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT1332.15280025898349No Hit
TATTGTGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG1061.7157656199417288No Hit
TATTGTGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT340.5503399158303658No Hit
TATTGTGGGTCAAAGATCCTCTTCTGAAGGAGATTGGGCG300.48559404337973455No Hit
TATTGTGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTGT300.48559404337973455No Hit
TATTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.38847523470378764No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.38847523470378764No Hit
TATTGTGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTT220.356102298478472No Hit
TATTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.2589834898025251No Hit
TATTGTGGGTCCCTGGTTGATCCTGCCAGTAGCATATGCT150.24279702168986728No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC150.24279702168986728No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGTCACAGATCC150.24279702168986728No Hit
TATTGTGGGTCACAGATCCTCTGAAGGAGATTGGGCGGAT120.19423761735189382No Hit
TATTGTGGGGTGCTTATTGTGGGTCACAGATCCTCTTCTG100.16186468112657817No Hit
TATTGTGGGACTAATCCTAGCCCTAGCCCTACACAAATAT100.16186468112657817No Hit
TATTGTGGGCAGAGTGCAGTGCTTATTGTGGGTCACAGAT90.14567821301392037No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG90.14567821301392037No Hit
TATTGTGGGTCACCGATCCTCTTCTGAAGGAGATTGGGCG90.14567821301392037No Hit
TATTGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC80.12949174490126256No Hit
TATTGTGGGTCACAGATACTCTTCTGAAGGAGATTGGGCG80.12949174490126256No Hit
TATTGTGGGCAGTGCTTATTGTGGGTCACAGATCCTCTTC80.12949174490126256No Hit
TATTGTGGGTCACAGATCCTCTTCTGAGATGCGTTTTTGT80.12949174490126256No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGTCACAGATCCTC70.11330527678860473No Hit
TATTGTGGGTCATAGATCCTCTTCTGAAGGAGATTGGGCG70.11330527678860473No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGTCACAGATCCT70.11330527678860473No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG454.5474735E-933.723589
ACCTGGT454.5474735E-933.7235811
GTGGGTA750.033.723585
GTGGGGG900.033.723585
TACCTGG454.5474735E-933.7235810
GGTACCT454.5474735E-933.723588
GGGTACC503.1468517E-1033.7235767
CTGGTTG406.531263E-833.72357613
TGGGGTT251.892671E-433.7235766
TGGGGTG200.00268522533.7235766
TGGGGGG359.3408016E-733.7235766
TGGGGAG359.3408016E-733.7235766
GTGGGGT503.1468517E-1033.7235765
GTGGGGC301.33135945E-533.7235765
GTGGGGA552.1827873E-1133.7235765
GTGGGAG301.33135945E-533.7235765
TGTGGGG2250.033.7235764
TGGGTAC503.1468517E-1033.7235766
TGGGTAA251.892671E-433.7235766
TGGGATT251.892671E-433.7235766
Produced by FastQC (version 0.11.5)