FastQCFastQC Report
Fri 17 Jun 2016
SRR1526114_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526114_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41910
Sequences flagged as poor quality0
Sequence length56
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG9762.32879980911477No Hit
GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG2800.6680983058935815No Hit
GCAGGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT2350.5607253638749702No Hit
GCAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA2020.48198520639465525No Hit
GCAGGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC1470.35075161059413024No Hit
GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1240.29587210689572896No Hit
GCAGGAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT1210.2887139107611548No Hit
GCAGGAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1040.24815079933190168No Hit
GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG990.23622047244094488No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC910.217131949415414No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC860.20520162252445714No Hit
GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG830.1980434263898831No Hit
GCAGGAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA660.15748031496062992No Hit
GCAGGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT600.14316392269148173No Hit
GCAGGAGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT530.1264614650441422No Hit
GCAGGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC510.12168933428775949No Hit
GCAGGAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT500.11930326890956812No Hit
GCAGGAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATC480.1145311381531854No Hit
GCAGGAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC430.10260081126222857No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCA251.9126488E-550.5990341
GACTGCC150.00798867650.59902641
CTCAATC150.00798867650.59902641
TGTCCAA203.9477018E-450.47636438
GTCCAAT251.940394E-550.47636439
GCCGATT251.940394E-550.47636438
CCGATTG251.940394E-550.47636439
TGCTCAA150.00806582950.4763639
CCACGAT150.00810461250.41525346
CTCCACG150.00810461250.41525344
TCAGAAG150.00810461250.41525346
TCCACGA150.00810461250.41525345
CTGCCCA150.00810461250.41525343
TACTATT150.00810461250.41525346
TTGTCCA204.0192157E-450.2934837
TTGTCCT150.008182595550.29347637
ATGCTCG204.043281E-450.2328136
TCCTCAC150.00822179650.23280734
CACAATG150.00826113650.17228747
CACGATT150.00826113650.17228747
Produced by FastQC (version 0.11.5)