##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526114_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 41910 Sequences flagged as poor quality 0 Sequence length 56 %GC 57 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.13977570985445 38.0 35.0 39.0 30.0 40.0 2 35.94108804581246 38.0 36.0 39.0 30.0 40.0 3 34.7969696969697 38.0 35.0 39.0 22.0 40.0 4 35.86370794559771 38.0 35.0 39.0 30.0 40.0 5 35.51388690050107 38.0 35.0 39.0 28.0 40.0 6 33.00875685993796 38.0 32.0 39.0 15.0 40.0 7 35.27065139584825 38.0 35.0 39.0 28.0 40.0 8 35.22794082557862 38.0 35.0 39.0 27.0 40.0 9 34.137318062514915 38.0 32.0 39.0 24.0 40.0 10 33.910021474588405 38.0 32.0 39.0 22.0 40.0 11 33.327248866618945 37.0 31.0 39.0 22.0 40.0 12 32.85748031496063 37.0 31.0 39.0 19.0 40.0 13 32.60534478644715 37.0 30.0 39.0 17.0 40.0 14 33.311620138391795 38.0 32.0 39.0 20.0 40.0 15 32.33977570985445 37.0 30.0 39.0 16.0 40.0 16 32.641350513004056 37.0 30.0 38.0 19.0 40.0 17 31.66709615843474 36.0 28.0 38.0 15.0 40.0 18 31.075232641374374 35.0 27.0 38.0 14.0 40.0 19 32.218754473872586 36.0 29.0 38.0 18.0 40.0 20 32.66862324027679 36.0 30.0 38.0 21.0 40.0 21 32.81314722023384 37.0 31.0 39.0 19.0 40.0 22 32.05993796230017 36.0 29.0 39.0 16.0 40.0 23 32.80909090909091 37.0 31.0 39.0 19.0 40.0 24 32.11507993319017 36.0 29.0 39.0 17.0 40.0 25 32.54075399665951 36.0 30.0 38.0 19.0 40.0 26 32.87668814125507 37.0 32.0 39.0 19.0 40.0 27 33.093820090670484 37.0 32.0 39.0 20.0 40.0 28 33.009067048437124 37.0 32.0 38.0 20.0 40.0 29 32.549176807444525 36.0 31.0 38.0 18.0 40.0 30 32.23214030064424 36.0 30.0 38.0 17.0 39.0 31 32.86945836315915 37.0 32.0 38.0 18.0 40.0 32 32.7758530183727 37.0 32.0 38.0 18.0 39.0 33 32.55573848723455 36.0 31.0 38.0 18.0 39.0 34 32.32932474349797 36.0 31.0 38.0 17.0 39.0 35 32.21321880219518 36.0 31.0 38.0 16.0 39.0 36 31.78730613218802 36.0 31.0 38.0 11.0 39.0 37 31.48990694345025 36.0 30.0 38.0 10.0 39.0 38 30.793939393939393 35.0 29.0 38.0 4.0 39.0 39 29.628752087807207 35.0 27.0 38.0 4.0 39.0 40 27.717060367454067 33.0 19.0 38.0 4.0 39.0 41 25.774779288952516 31.0 15.0 37.0 4.0 39.0 42 26.4226914817466 31.0 18.0 37.0 4.0 39.0 43 26.55099021713195 32.0 18.0 37.0 4.0 39.0 44 25.56984013361966 31.0 14.0 36.0 4.0 39.0 45 26.406824146981627 32.0 18.0 37.0 4.0 39.0 46 24.220496301598665 29.0 10.0 35.0 4.0 38.0 47 23.3728227153424 28.0 7.0 35.0 4.0 38.0 48 23.93715103793844 29.0 9.0 35.0 4.0 38.0 49 22.960081126222857 27.0 4.0 35.0 4.0 38.0 50 23.515366261035552 28.0 4.0 35.0 4.0 38.0 51 23.737222619899786 29.0 4.0 35.0 4.0 38.0 52 23.568742543545692 29.0 4.0 35.0 4.0 38.0 53 23.407993319016942 29.0 4.0 35.0 4.0 38.0 54 23.040181340968743 29.0 4.0 35.0 4.0 38.0 55 22.72584108804581 29.0 4.0 35.0 4.0 38.0 56 22.091577189214984 28.0 4.0 35.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 4.216363273537922 1 2 4.519420924052191 1 3 6.002552442318404 1 4 4.558299159581047 1 5 4.847601743919071 1 6 7.90336195289019 1 7 4.83211939486273 1 8 5.503219527085655 1 9 6.017951452472346 1 10 5.787464561250406 1 11 -0.5322185334867413 1 12 -6.6760093299618575 1 13 -18.21904275067726 1 14 -6.5726069291428075 1 15 2.304562617371797 1 16 -0.6755902914632941 1 17 -4.144960172577594 1 18 -5.650312835965085 1 19 0.4146646384939068 1 20 -0.6632894268071681 1 21 6.682636445918014 1 22 7.607587451889916 1 23 5.6540812918365795 1 24 6.352450406330288 1 25 5.899865159973629 1 26 6.000144244981897 1 27 5.777268501292003 1 28 6.632960050296745 1 29 6.400935279732181 1 30 5.6254348553267945 1 31 5.4760437849586125 1 32 4.662507405973127 1 33 4.73819036684846 1 34 4.878706357194645 1 35 5.3234016512823175 1 36 5.614049726560662 1 37 4.4234963186615985 1 38 5.649844689110363 1 39 4.936536769870443 1 40 -11.500369899459784 1 41 -0.7420918765040874 1 42 -2.276046607958385 1 43 -9.986287027895676 1 44 -15.411600306671787 1 45 -4.559180454890928 1 46 -6.635289247412361 1 47 -10.882961130440119 1 48 -5.316597524607701 1 49 -12.659502296859715 1 50 -4.956891149508637 1 51 1.351858528180344 1 52 1.180189243456791 1 53 1.3781189676562988 1 54 1.4198408312809576 1 55 1.515724727211179 1 56 4.818616740960305 2 1 4.2006950707729445 2 2 4.099605255849426 2 3 6.173059354760802 2 4 3.956455841608694 2 5 5.077094831476671 2 6 7.114421860724288 2 7 5.193870546936459 2 8 5.622113536302244 2 9 6.377398457080645 2 10 5.700828616549945 2 11 4.039670867434918 2 12 -0.5400646295010247 2 13 1.0288835166038481 2 14 5.527393070857194 2 15 5.807327594330321 2 16 4.513349800702603 2 17 6.599740288251898 2 18 2.973189468182383 2 19 5.81051717305612 2 20 4.986940987939381 2 21 6.779410639466398 2 22 7.404361645438303 2 23 7.074357789532431 2 24 6.4695010975745255 2 25 6.966685436471323 2 26 6.674337793368991 2 27 6.233489699448686 2 28 4.850010741540984 2 29 5.776511316598544 2 30 5.653084624912047 2 31 5.351158992331882 2 32 4.637161783853312 2 33 4.449250274682559 2 34 4.818337693600171 2 35 5.617410867872174 2 36 5.83616954222887 2 37 6.566814291011831 2 38 6.577033629202528 2 39 7.04068423530823 2 40 2.8116116673604914 2 41 7.642700750224023 2 42 6.606441871304288 2 43 5.518321267035201 2 44 -4.456761596994365 2 45 6.690589130362525 2 46 6.199733794062293 2 47 2.8460711276243984 2 48 5.514278051429166 2 49 -1.9940645088412836 2 50 6.862463689201039 2 51 7.9334253484568436 2 52 8.943322884009785 2 53 8.918211133554916 2 54 8.674679540958376 2 55 9.644756985275695 2 56 8.373916280130814 3 1 3.724788887404074 3 2 4.2546159168302395 3 3 5.5803088217117605 3 4 3.7138119183677603 3 5 3.9788005884063153 3 6 7.238942116587825 3 7 4.883636005358639 3 8 5.353243600268129 3 9 6.4093813995113464 3 10 5.305519448106448 3 11 5.544574918607626 3 12 5.522835157279356 3 13 6.0137449238533165 3 14 5.897542324588535 3 15 0.4668158672514302 3 16 6.6530086493166785 3 17 6.706989755202859 3 18 7.7885412805491185 3 19 6.372777300987892 3 20 6.154958045508675 3 21 7.51075392304849 3 22 7.719287018572626 3 23 7.3091125869738 3 24 7.716196193523356 3 25 7.019990340522497 3 26 7.072845256055558 3 27 6.922189059789833 3 28 5.88476553898235 3 29 7.121927094850143 3 30 6.427071831735077 3 31 5.015764536041907 3 32 6.461255600484446 3 33 5.82003918726252 3 34 5.706823834325121 3 35 6.623594236742115 3 36 6.956851845000724 3 37 7.022677830457461 3 38 7.643344929842183 3 39 8.903797241704819 3 40 8.958946422157933 3 41 11.686410771545983 3 42 11.574458928873586 3 43 11.289110179615161 3 44 11.002939895542948 3 45 9.585258639957406 3 46 12.596535499819225 3 47 12.85801142613186 3 48 11.768862273177565 3 49 13.095914169197094 3 50 12.671418913081634 3 51 11.653254772763876 3 52 12.626265314713407 3 53 12.39497019538861 3 54 12.431395958868823 3 55 12.070130119604052 3 56 11.906965320642538 4 1 3.629266499344368 4 2 3.4996052558494313 4 3 5.115916497617945 4 4 3.756455841608698 4 5 3.834237688619531 4 6 6.842993289295716 4 7 4.265299118365036 4 8 4.879256393445104 4 9 5.6916841713663615 4 10 4.786542902264234 4 11 6.196813724577776 4 12 6.488506799070404 4 13 6.043169230889561 4 14 5.9273930708571925 4 15 5.821613308616033 4 16 6.827635514988319 4 17 7.428311716823327 4 18 8.158903753896666 4 19 6.4533743159132655 4 20 6.415512416510804 4 21 6.950839210894969 4 22 7.047218788295442 4 23 5.531500646675294 4 24 6.798072526145955 4 25 5.995256865042755 4 26 5.517194936226133 4 27 5.147775413734397 4 28 5.1500107415409815 4 29 5.090797030884261 4 30 5.124513196340622 4 31 3.8511589923318823 4 32 4.422876069567597 4 33 4.263535988968275 4 34 4.1326234078858874 4 35 5.160268010729318 4 36 5.664740970800292 4 37 5.995385719583261 4 38 5.705605057773955 4 39 4.769255663879658 4 40 -1.7883883326395065 4 41 8.242700750224024 4 42 6.992156157018574 4 43 7.661178409892344 4 44 8.386095545862776 4 45 7.247731987505382 4 46 10.299733794062295 4 47 11.003213984767253 4 48 10.12856376571488 4 49 9.57736406258729 4 50 10.09103511777247 4 51 8.633425348456843 4 52 6.714751455438357 4 53 7.918211133554916 4 54 7.946108112386948 4 55 8.116185556704266 4 56 8.188201994416527 5 1 4.285148852285545 5 2 4.206327944925057 5 3 5.362134985012901 5 4 4.115699539087686 5 5 4.752725083577516 5 6 7.1619008523209295 5 7 5.195971387272593 5 8 5.38177740184846 5 9 6.5895833310302265 5 10 6.279400045121374 5 11 5.655637253989543 5 12 6.359095034364518 5 13 6.656194440973593 5 14 6.368569541445424 5 15 7.68169734222948 5 16 6.609988456164789 5 17 6.782933565562825 5 18 8.06268526650171 5 19 6.488668433560321 5 20 6.621814937519208 5 21 7.0441165218193404 5 22 6.792596939555946 5 23 6.302088881969407 5 24 7.111938072364442 5 25 6.786013167563766 5 26 6.494926028663109 5 27 5.239792220457087 5 28 4.598330069272073 5 29 6.091637367018713 5 30 6.6908997509624655 5 31 4.581831261239444 5 32 4.0951449771306265 5 33 5.643788089808613 5 34 4.3578334919195 5 35 3.4695117082083087 5 36 4.807598113657438 5 37 4.302528576726118 5 38 5.792159679622696 5 39 6.583121210098145 5 40 6.88388057492352 5 41 10.088078901484526 5 42 9.011904056178235 5 43 7.9952120233377215 5 44 9.222650167711517 5 45 8.33218576901799 5 46 10.244691777255571 5 47 9.628424068800868 5 48 9.299152001008995 5 49 10.118960701242752 5 50 9.535993100965747 5 51 9.602332911482051 5 52 9.431138010060206 5 53 9.471572478092732 5 54 9.8187971880172 5 55 9.468286397040401 5 56 8.317193591055183 6 1 4.460035730113603 6 2 4.46334151958569 6 3 5.975257156958605 6 4 3.7344778196306763 6 5 4.390281644663489 6 6 7.523213069515496 6 7 4.66090351396943 6 8 4.755080569269275 6 9 6.093881973564159 6 10 5.729400045121377 6 11 5.142967570731624 6 12 5.8192760298396315 6 13 7.069542857263187 6 14 5.9273930708571925 6 15 7.140294627297354 6 16 6.743020130372937 6 17 7.055784244295854 6 18 8.338024633017547 6 19 7.607220469759419 6 20 6.76276516376355 6 21 7.071718331774093 6 22 7.888977030053685 6 23 7.000731415906063 6 24 6.134336262409693 6 25 6.2480041177900105 6 26 4.851260870292073 6 27 3.857665523624508 6 28 3.622538214068456 6 29 3.508379448466677 6 30 4.6442934161208385 6 31 3.4313787725516605 6 32 2.781117827809357 6 33 3.558041483473769 6 34 2.5447113199738 6 35 3.9921361425974524 6 36 3.8075981136574377 6 37 4.852528576726115 6 38 4.916594068762965 6 39 4.974750169374165 6 40 3.285238040986865 6 41 9.661382068905343 6 42 7.843804508666928 6 43 8.673266321980254 6 44 8.620161479928711 6 45 8.74553418530758 6 46 9.701931596260096 6 47 10.476840358393627 6 48 10.443948381099496 6 49 9.755386040609267 6 50 10.40092522766258 6 51 9.22133743636893 6 52 8.1576085982955 6 53 8.48084849619228 6 54 7.836218002496839 6 55 7.960141600660311 6 56 6.956333862548394 7 1 3.708054378132246 7 2 4.3195186757628505 7 3 5.744487926189372 7 4 3.996715581868436 7 5 4.529908684290525 7 6 7.121348267650699 7 7 4.501229854295765 7 8 5.384451198639908 7 9 6.390385470067656 7 10 6.150612166333499 7 11 6.278631906395955 7 12 6.585476496040098 7 13 6.73061511833545 7 14 6.9273930708571925 7 15 7.44239252939526 7 16 6.939756727109529 7 17 7.68935067786229 7 18 7.975786870779785 7 19 7.329131891670841 7 20 6.583044884043272 7 21 6.476380336436094 7 22 6.674058615135269 7 23 5.770894586069229 7 24 7.338765166838595 7 25 6.3065122762981645 7 26 5.274337793368993 7 27 5.6404161063750955 7 28 5.240486932017177 7 29 5.59252863261586 7 30 5.958279430106856 7 31 5.159817000989889 7 32 5.899066545758078 7 33 5.047085772518056 7 34 3.945177520439998 7 35 4.433861084322395 7 36 5.519719325778649 7 37 6.779801303998845 7 38 6.5203236724925695 7 39 8.355402850026845 7 40 7.0211354768843 7 41 9.302873910397185 7 42 9.351030615893034 7 43 9.57676282547676 7 44 10.746268706035938 7 45 9.285394325167719 7 46 11.898435092763592 7 47 12.321828703381975 7 48 10.146745583896696 7 49 12.2842904695137 7 50 10.765493992231345 7 51 10.1905682055997 7 52 9.675790416477316 7 53 9.906955722299507 7 54 9.820134086412924 7 55 9.190211530730242 7 56 9.454002860217393 8 1 4.33695880703668 8 2 4.324879981124155 8 3 5.929103310804756 8 4 4.4575547427076 8 5 5.1287431831250245 8 6 7.646289992592418 8 7 5.383980437046354 8 8 5.6935421077308135 8 9 6.586189665871849 8 10 6.467861583582913 8 11 6.650659878423927 8 12 7.419276029839633 8 13 6.9310813188016525 8 14 6.573546917011036 8 15 8.171063858066585 8 16 6.527635514988315 8 17 7.471168859680471 8 18 8.03033232532524 8 19 7.945682008220956 8 20 6.624303625302016 8 21 7.010179870235632 8 22 7.827438568515223 8 23 7.339192954367601 8 24 7.303567031640458 8 25 7.09415796394385 8 26 7.3435685625997635 8 27 6.057665523624511 8 28 5.976384367914612 8 29 6.8006871407743645 8 30 7.305831877659301 8 31 4.677532618705513 8 32 5.273425520117048 8 33 5.050349175781459 8 34 4.621634396896873 8 35 5.038289988751295 8 36 5.422982729042051 8 37 4.60637473057227 8 38 6.116594068762968 8 39 7.143980938604937 8 40 2.008314964063789 8 41 11.9229205304438 8 42 9.489958354820775 8 43 10.073266321980253 8 44 11.112469172236406 8 45 9.945534185307583 8 46 13.317316211644709 8 47 13.553763435316704 8 48 12.751640688791799 8 49 13.632309117532344 8 50 12.847079073816428 8 51 12.421337436368933 8 52 10.465300905987807 8 53 11.665463880807664 8 54 11.497756464035302 8 55 10.867833908352619 8 56 10.23325693947147 9 1 4.5959331660110365 9 2 4.237700493944665 9 3 5.71115459285604 9 4 4.375503460656319 9 5 5.2223329267147705 9 6 7.116802813105242 9 7 5.05577530884122 9 8 5.093542107730812 9 9 6.429779409461595 9 10 6.412733378454707 9 11 6.61348039124444 9 12 7.355173465737071 9 13 7.333645421365752 9 14 6.460726404190524 9 15 7.545422832425562 9 16 7.560968848321654 9 17 8.354502193013804 9 18 8.730332325325236 9 19 7.403374315913261 9 20 7.151226702225092 9 21 7.079410639466396 9 22 7.504361645438298 9 23 6.23150064667529 9 24 7.476643954717385 9 25 5.876209245995135 9 26 5.524337793368993 9 27 5.569203985162968 9 28 5.107153598683844 9 29 5.945558935646162 9 30 5.48403700586443 9 31 4.7083018494747435 9 32 4.18239987909141 9 33 5.284964560396848 9 34 3.9421472174096976 9 35 4.945982296443603 9 36 6.740931446990768 9 37 6.602528576726115 9 38 5.679414581583476 9 39 6.831160425784422 9 40 2.7044688102176373 9 41 9.18317694070021 9 42 9.037394252256671 9 43 8.854035552749483 9 44 9.943238403005633 9 45 9.626303416076812 9 46 10.771162365490866 9 47 11.246071127624397 9 48 10.645230432381545 9 49 10.529745014968242 9 50 11.345797022534374 9 51 10.173901538933034 9 52 10.790941931628833 9 53 11.02059208593587 9 54 12.258012874291712 9 55 11.728090318609027 9 56 10.335821042035576 10 1 4.442169725150819 10 2 4.34200156921348 10 3 5.647713732640987 10 4 4.558299159581047 10 5 4.4121178729513275 10 6 7.354974856115991 10 7 4.7837322980885375 10 8 4.390316301279199 10 9 5.95343532344009 10 10 4.577787141895566 10 11 5.854878240706807 10 12 6.9368938958445945 10 13 7.651924991258227 10 14 6.056425328921712 10 15 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3.5852366483040043 115 35 4.6825676622972665 115 36 6.417354211218413 115 37 6.564723698677341 115 38 6.201772305160713 115 39 5.878314897329137 115 40 8.317883444363975 115 41 8.585209461025418 115 42 7.6865812441265895 115 43 4.913791650310461 115 44 -0.5445664750431476 115 45 6.40069365997925 115 46 3.1345769996372077 115 47 -6.583197165058531 115 48 1.0724662047392677 115 49 -5.532043602917938 115 50 1.9014880794449418 115 51 2.312519425111894 115 52 3.0161451836613544 115 53 3.042949809513104 115 54 2.2137039312022786 115 55 2.827683814543988 115 56 6.387853562360778 116 1 4.155797111589266 116 2 3.9506256640126907 116 3 5.887345069046518 116 4 4.144210943649512 116 5 4.728115239639941 116 6 6.551156554601842 116 7 4.469380751018093 116 8 5.3139502709961235 116 9 6.17331682442758 116 10 5.570216371651984 116 11 4.0151810715165475 116 12 3.898710880703053 116 13 4.292148822726297 116 14 4.111066540244948 116 15 -3.941651997506412 116 16 4.360288576212806 116 17 6.2997402882519005 116 18 5.185434366141564 116 19 5.926843703668368 116 20 5.49306343691897 116 21 5.493696353752114 116 22 6.27579021686687 116 23 4.7294598303487625 116 24 7.320521505737791 116 25 5.476889518103981 116 26 5.1008684056138875 116 27 5.2130815361833776 116 28 4.529602578275679 116 29 4.429572541088341 116 30 4.000023400422251 116 31 4.549118176005351 116 32 4.035120967526783 116 33 4.773740070600926 116 34 2.2203785099267037 116 35 3.7582271944027923 116 36 4.521883827943153 116 37 5.3994673522363215 116 38 4.885196894508649 116 39 6.93456178632864 116 40 7.62997901429927 116 41 7.163108913489328 116 42 5.912564320283881 116 43 1.5489335119331606 116 44 2.188136362189308 116 45 4.27426259975028 116 46 0.4262644063071903 116 47 -5.345765607069481 116 48 -0.19184439755042604 116 49 -1.4797787945555676 116 50 2.3196065463438984 116 51 3.619139634171127 116 52 4.480057577887337 116 53 3.0733131743712434 116 54 3.427740765448174 116 55 3.736593719969573 116 56 5.353508116865505 117 1 3.6149807850586555 117 2 3.6853195415637146 117 3 5.339726021427467 117 4 3.6850272701801288 117 5 4.248523402905249 117 6 6.9525170988195235 117 7 4.098632451698364 117 8 4.484018298207005 117 9 4.686922266604455 117 10 4.457971473692805 117 11 -0.16509103732698804 117 12 -6.230540819977218 117 13 -10.752068864348534 117 14 -1.691654548190428 117 15 -7.18314859614587 117 16 -6.605697818345014 117 17 0.9425974311090428 117 18 -3.1172867222938088 117 19 1.2295647921037371 117 20 -0.12258282158443379 117 21 5.1841725442283035 117 22 5.990075931152585 117 23 5.188643503818145 117 24 5.602834430907858 117 25 5.871447341233228 117 26 5.202909221940423 117 27 5.09063255659154 117 28 4.835725027255272 117 29 5.466987507074734 117 30 5.748322720150142 117 31 4.532111373284266 117 32 4.208590355281888 117 33 4.692107417539699 117 34 5.451671026933504 117 35 4.455506105967416 117 36 6.759979066038387 117 37 6.481100005297549 117 38 7.072271724440622 117 39 7.550208044832036 117 40 10.092564048312877 117 41 9.49984360736688 117 42 9.868346633209054 117 43 -2.9340596853457512 117 44 -0.9853330255657937 117 45 7.42392246369586 117 46 0.48068617501467514 117 47 2.674642556195824 117 48 3.757135194286306 117 49 1.496411681634907 117 50 2.0338922606296137 117 51 7.928663443694937 117 52 8.000465741152642 117 53 7.84202065736444 117 54 8.91753668381552 117 55 7.335233175751885 117 56 11.278678184892716 118 1 3.484438913137474 118 2 3.5572407238297217 118 3 5.158281029637649 118 4 3.593894265254015 118 5 3.8815283290136193 118 6 7.4484120085075425 118 7 4.329338527232025 118 8 4.396990383592886 118 9 4.69759550141562 118 10 4.150089700293787 118 11 -1.972151792663606 118 12 -7.335631131964082 118 13 -7.161756877484823 118 14 -8.555365549832462 118 15 -13.955726592861797 118 16 -11.168916209149614 118 17 -2.9564173472160817 118 18 -10.086909053985107 118 19 -2.6431774082246697 118 20 -1.6504974357059439 118 21 2.089755467052605 118 22 3.4284995764727846 118 23 3.3142592673649496 118 24 3.1438853340277255 118 25 3.9026460276043338 118 26 5.326061931300025 118 27 4.657135019645732 118 28 5.407153598683841 118 29 3.717398016105932 118 30 6.298404821956382 118 31 3.887612194302328 118 32 4.772629764148878 118 33 4.869447319017535 118 34 3.9065150335016483 118 35 4.111499537822915 118 36 5.7041498377953666 118 37 6.397356162933015 118 38 6.452403087330605 118 39 6.856447782106262 118 40 9.02688260332108 118 41 6.344671193573777 118 42 5.458658620072761 118 43 1.0626562424046568 118 44 -0.8498650452702279 118 45 3.9280275540078478 118 46 0.05047271031845213 118 47 -1.2366874930652578 118 48 0.7251154898528114 118 49 -2.0610595827329092 118 50 1.8762567926493183 118 51 6.431947515944529 118 52 7.11622928795067 118 53 6.431511626165754 118 54 7.291920920268723 118 55 7.868894916310179 118 56 11.354211846633277 119 1 3.4878030847102224 119 2 3.6051801687414127 119 3 5.378634267652785 119 4 3.4324140297620076 119 5 4.312983333218838 119 6 6.60989224399955 119 7 4.829759048678625 119 8 5.074029912608864 119 9 5.19969810864859 119 10 4.67574150853601 119 11 0.9260820172607005 119 12 -5.5139322253198415 119 13 -0.25090742416270473 119 14 -3.0726069291428075 119 15 -7.2139267610703754 119 16 -8.540657167938512 119 17 1.407754225534127 119 18 -4.057472552723542 119 19 0.10703285249863015 119 20 -0.9280415904578341 119 21 3.974532590685911 119 22 5.2653372551943995 119 23 4.807110402772853 119 24 3.5290829791076312 119 25 4.719298676889444 119 26 5.945069500686067 119 27 6.202130814431261 119 28 4.553495062098477 119 29 6.281737797434779 119 30 5.9527361928563 119 31 5.4839116055723025 119 32 4.980367358766205 119 33 5.639842609177332 119 34 6.121822014157665 119 35 5.706957906199705 119 36 6.831988357559876 119 37 6.881796869409044 119 38 5.10421132955096 119 39 5.951485629036451 119 40 6.073981005339586 119 41 6.073014339074199 119 42 4.63780075632171 119 43 -3.4520620082261253 119 44 0.06518962251782767 119 45 3.254352196564618 119 46 -0.25566690280181703 119 47 -1.4856361894487726 119 48 -6.073875258675365 119 49 -0.09301921267403657 119 50 0.9502685672498217 119 51 6.41008040072165 119 52 7.284437866588181 119 53 7.5063644436594466 119 54 5.652728321446183 119 55 7.217927716983013 119 56 7.875658440409559 120 1 3.5984972685751373 120 2 3.791546647790817 120 3 5.565000746702189 120 4 2.9396060247588807 120 5 3.99028164466349 120 6 7.077059223361655 120 7 4.225006078071992 120 8 4.832003646192355 120 9 5.355420435102623 120 10 3.8883744040957318 120 11 0.5070701348341835 120 12 -4.919185508621904 120 13 -4.325328937608607 120 14 -3.8418376983735776 120 15 0.9505510375537654 120 16 -7.759543972191167 120 17 -0.6672926787810702 120 18 -4.6722317772388635 120 19 -0.1312410687021206 120 20 -1.1500553490569594 120 21 1.8306926907484495 120 22 4.499233440310096 120 23 3.0109878261624736 120 24 3.3189516470250773 120 25 3.8787733485592355 120 26 3.0820301010612994 120 27 2.6986911646501532 120 28 3.5609997525299946 120 29 3.626328166415391 120 30 2.8801908520182735 120 31 3.251891593064485 120 32 2.1298357765273046 120 33 2.1682978937301804 120 34 2.5652241404866203 120 35 2.5818797323410436 120 36 2.7563160623753866 120 37 2.821759345956888 120 38 3.5319786841475818 120 39 4.559365553989547 120 40 5.469853425602253 120 41 -8.384771777248504 120 42 -7.83311856825615 120 43 -10.798528549814616 120 44 -14.287530827763597 120 45 -6.905747865974469 120 46 -9.380119685791186 120 47 -6.882134000580731 120 48 -8.812461875310762 120 49 -13.747178061954836 120 50 -7.229844003106651 120 51 -2.8350728200413258 120 52 -3.514186273499373 120 53 -3.8319720166282316 120 54 -3.35352558724675 120 55 -3.2398583993396883 120 56 -0.6077687015541677 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1190.0 5 183.0 6 106.0 7 104.0 8 89.0 9 103.0 10 116.0 11 98.0 12 90.0 13 100.0 14 165.0 15 188.0 16 212.0 17 264.0 18 356.0 19 459.0 20 561.0 21 801.0 22 911.0 23 1086.0 24 1202.0 25 1338.0 26 1416.0 27 1481.0 28 1518.0 29 1666.0 30 1889.0 31 2037.0 32 2188.0 33 2488.0 34 2781.0 35 2933.0 36 3200.0 37 3533.0 38 3020.0 39 2038.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.45597709377238 0.3435934144595562 0.18134096874254355 0.0190885230255309 8 99.49415413982344 0.329277022190408 0.10975900739680267 0.06680983058935815 9 98.93342877594846 0.5368647100930566 0.3340491529467908 0.1956573610116917 10 66.30159866380339 11.906466237174898 14.314006203769983 7.47792889525173 11 39.48938200906705 26.912431400620378 13.965640658554044 19.63254593175853 12 30.41994750656168 18.869005010737293 19.31997136721546 31.391076115485568 13 23.18778334526366 15.602481507993318 22.502982581722737 38.70675256502028 14 21.88976377952756 22.068718682891912 30.45573848723455 25.58577905034598 15 31.592313958503045 17.65297810149025 24.905362020221382 25.84934591978533 16 33.4136967989879 21.597880314133626 21.757811567564985 23.23061131931349 17 28.435724855601702 18.53071745668051 32.383407322545224 20.65015036517256 18 25.03937007874016 18.01956573610117 29.611071343354805 27.329992841803865 19 31.89772483109318 19.232697495643038 21.3192637333779 27.550313939885886 20 28.37311542780685 24.954005686569662 24.409241870355768 22.263637015267722 21 25.707468384633742 20.114531138153186 27.649725602481507 26.528274874731565 22 23.69124314006204 18.546886184681462 28.131710808876164 29.63015986638034 23 23.99188737771415 19.38678119780482 26.16559293724648 30.45573848723455 24 24.248932159305127 20.3880020044384 27.248431049705303 28.114634786551175 25 30.204977688691624 20.8771804233183 25.246378886582193 23.67146300140788 26 29.879679144385022 19.891138273491215 21.949006875477465 28.280175706646293 27 24.986267344940412 23.140120847364525 19.90876740464761 31.964844403047454 28 25.86856924692183 27.498329674525152 20.70487735038656 25.92822372816646 29 34.624303712735184 23.194434483253247 19.41476009467116 22.76650170934041 30 28.03925782649187 20.43365093010483 29.254722162523585 22.27236908087972 31 29.103622738771417 26.354350627655005 22.163619874946303 22.37840675862727 32 36.026060186621486 22.71913705462616 19.81481039543708 21.439992363315273 33 31.390070244182155 21.67534763702394 20.344530988674915 26.590051130118987 34 25.445894897910392 25.47936690097069 27.239037918997756 21.835700282121167 35 25.07409886222392 22.47824839850846 33.31102399847022 19.1366287407974 36 27.21694283940595 27.31006160164271 24.136860703882338 21.336134855069 37 27.21514448932763 22.071372230322442 28.680354709945743 22.033128570404187 38 32.58128573821087 22.78393186113836 23.767256023159558 20.86752637749121 39 29.38997873408043 22.063988913048675 19.9756278225132 28.570404530357695 40 24.06222948927575 16.952005386169088 25.288544772530535 33.69722035202462 41 20.12148091014269 15.12003470883147 24.590242961820287 40.16824141920555 42 27.578448502836356 22.686038440364776 23.485483137461404 26.250029919337464 43 21.664275466284074 19.136776661884266 30.296508847441416 28.90243902439024 44 21.701862759182113 22.00833213618733 24.496001532346884 31.793803572283675 45 25.235030978637894 20.88366863622228 30.229887807095185 23.651412578044635 46 22.010095935309458 23.062752697432952 25.120218187037967 29.806933180219623 47 18.580360779052775 24.93591739932444 23.580001437366743 32.90372038425604 48 25.462056449687186 25.56234777209991 24.72181097473614 24.253784803476766 49 22.30910873568166 26.140086567663868 23.153263026998587 28.39754166965588 50 28.3217368433637 22.566530378021664 24.178083829471824 24.933648949142817 51 22.79550841573491 27.028515335073145 25.395647281346516 24.780328967845428 52 21.96853782155494 24.856555417423735 29.57827292722578 23.596633833795543 53 22.071985832575503 23.175226152299814 30.56526109223185 24.187526922892836 54 23.156031761216873 25.770113842915908 27.621257055390796 23.45259734047642 55 21.819792215253507 25.678651792981285 27.12213338440178 25.379422607363434 56 26.60063163939133 21.411139821992535 27.158101253708487 24.830127284907647 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 0.5 11 0.0 12 5.0 13 13.5 14 17.0 15 26.5 16 36.0 17 31.5 18 27.0 19 37.0 20 47.0 21 46.5 22 57.0 23 68.0 24 67.5 25 67.0 26 69.5 27 72.0 28 91.5 29 114.0 30 117.0 31 117.5 32 118.0 33 148.0 34 178.0 35 215.5 36 253.0 37 324.5 38 435.0 39 474.0 40 518.5 41 563.0 42 634.5 43 706.0 44 799.5 45 893.0 46 1178.5 47 2480.0 48 3496.0 49 3348.0 50 3200.0 51 2658.5 52 2117.0 53 2130.0 54 2250.0 55 2357.0 56 2422.5 57 2488.0 58 2527.5 59 2567.0 60 2690.5 61 2814.0 62 2922.5 63 2973.0 64 2915.0 65 2551.0 66 2187.0 67 2051.5 68 1916.0 69 1675.5 70 1435.0 71 1308.5 72 1029.5 73 877.0 74 767.5 75 658.0 76 525.5 77 393.0 78 300.5 79 166.0 80 124.0 81 108.0 82 92.0 83 62.0 84 32.0 85 22.0 86 12.0 87 7.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.4104032450489143 16 0.04056311142925316 17 0.02863278453829635 18 0.0 19 0.05487950369840133 20 0.13600572655690765 21 0.0 22 0.0 23 0.0 24 0.0071581961345740875 25 0.0071581961345740875 26 0.05249343832020997 27 0.09305654974946313 28 0.004772130756382725 29 0.19327129563350037 30 0.07874015748031496 31 0.0190885230255309 32 0.016702457647339537 33 0.1336196611787163 34 0.20042949176807445 35 0.17656883798616083 36 0.06680983058935815 37 0.17418277260796947 38 0.269625387735624 39 0.1407778573132904 40 0.7683130517776188 41 1.0069195895967549 42 0.3125745645430685 43 0.21474588403722264 44 0.3435934144595562 45 0.2553089954664758 46 0.2648532569792412 47 0.39847291815795755 48 0.0763540921021236 49 0.2219040801717967 50 0.20758768790264853 51 0.34120734908136485 52 0.1956573610116917 53 0.29587210689572896 54 0.23383440706275352 55 0.3245048914340253 56 0.2720114531138153 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 41910.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.34764972560248 #Duplication Level Percentage of deduplicated Percentage of total 1 88.88093689004555 65.1920782629444 2 6.652569941444372 9.759007396802673 3 1.9128171763175015 4.209019327129564 4 0.8425504229017567 2.4719637318062517 5 0.41964866623292124 1.5390121689334286 6 0.302537410540013 1.3314244810307803 7 0.18867924528301888 0.9687425435456932 8 0.1431359791802212 0.8398950131233596 9 0.11385816525699415 0.7516105941302791 >10 0.49121665582303187 6.308756859937962 >50 0.026024723487312947 1.4053925077547125 >100 0.02277163305139883 2.8942973037461224 >500 0.0032530904359141183 2.32879980911477 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 976 2.32879980911477 No Hit GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 280 0.6680983058935815 No Hit GCAGGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATCT 235 0.5607253638749702 No Hit GCAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA 202 0.48198520639465525 No Hit GCAGGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGGATTGAACAAAAACTC 147 0.35075161059413024 No Hit GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 124 0.29587210689572896 No Hit GCAGGAGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 121 0.2887139107611548 No Hit GCAGGAGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 104 0.24815079933190168 No Hit GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 99 0.23622047244094488 No Hit GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 91 0.217131949415414 No Hit GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 86 0.20520162252445714 No Hit GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 83 0.1980434263898831 No Hit GCAGGAGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 66 0.15748031496062992 No Hit GCAGGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTCCAATCCGCCCAATCT 60 0.14316392269148173 No Hit GCAGGAGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT 53 0.1264614650441422 No Hit GCAGGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCGCATTGAACAAAAACTC 51 0.12168933428775949 No Hit GCAGGAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT 50 0.11930326890956812 No Hit GCAGGAGGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGTTCAATCCGCCCAATC 48 0.1145311381531854 No Hit GCAGGAGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 43 0.10260081126222857 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.004772130756382725 0.0 0.0 0.0 0.0 7 0.004772130756382725 0.0 0.0 0.0 0.0 8 0.004772130756382725 0.0 0.0 0.0 0.0 9 0.004772130756382725 0.0 0.0 0.0 0.0 10 0.004772130756382725 0.0 0.0 0.0 0.0 11 0.004772130756382725 0.0 0.0 0.0 0.0 12 0.0071581961345740875 0.0 0.0 0.0 0.0 13 0.0071581961345740875 0.0 0.0 0.0 0.0 14 0.016702457647339537 0.0 0.0 0.0 0.0 15 0.016702457647339537 0.0 0.0 0.0 0.0 16 0.016702457647339537 0.0 0.0 0.0 0.0 17 0.016702457647339537 0.0 0.0 0.0 0.0 18 0.016702457647339537 0.0 0.0 0.0 0.0 19 0.0190885230255309 0.0 0.0 0.0 0.0 20 0.0190885230255309 0.0 0.0 0.0 0.0 21 0.021474588403722263 0.0 0.0 0.0 0.0 22 0.023860653781913623 0.0 0.0 0.0 0.0 23 0.023860653781913623 0.0 0.0 0.0 0.0 24 0.02863278453829635 0.0 0.0 0.0 0.0 25 0.03340491529467907 0.0 0.0 0.0 0.0 26 0.04056311142925316 0.0 0.0 0.0 0.0 27 0.045335242185635886 0.0 0.0 0.0 0.0 28 0.05487950369840133 0.0 0.0 0.0 0.0 29 0.05487950369840133 0.0 0.0 0.0 0.0 30 0.05487950369840133 0.0 0.0 0.0 0.0 31 0.06680983058935815 0.0 0.0 0.0 0.0 32 0.07396802672393224 0.0 0.0 0.0 0.0 33 0.07396802672393224 0.0 0.0 0.0 0.0 34 0.07396802672393224 0.0 0.0 0.0 0.0 35 0.0763540921021236 0.0 0.0 0.0 0.0 36 0.07874015748031496 0.0 0.0 0.0 0.0 37 0.08112622285850632 0.0 0.0 0.0 0.0 38 0.08112622285850632 0.0 0.0 0.0 0.0 39 0.08351228823669768 0.0 0.0 0.0 0.0 40 0.08589835361488905 0.0 0.0 0.0 0.0 41 0.08589835361488905 0.0 0.0 0.0 0.0 42 0.08828441899308041 0.0 0.0 0.0 0.0 43 0.09305654974946313 0.0 0.0 0.0 0.0 44 0.09782868050584587 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCTCA 25 1.9126488E-5 50.59903 41 GACTGCC 15 0.007988676 50.599026 41 CTCAATC 15 0.007988676 50.599026 41 TGTCCAA 20 3.9477018E-4 50.476364 38 GTCCAAT 25 1.940394E-5 50.476364 39 GCCGATT 25 1.940394E-5 50.476364 38 CCGATTG 25 1.940394E-5 50.476364 39 TGCTCAA 15 0.008065829 50.47636 39 CCACGAT 15 0.008104612 50.415253 46 CTCCACG 15 0.008104612 50.415253 44 TCAGAAG 15 0.008104612 50.415253 46 TCCACGA 15 0.008104612 50.415253 45 CTGCCCA 15 0.008104612 50.415253 43 TACTATT 15 0.008104612 50.415253 46 TTGTCCA 20 4.0192157E-4 50.29348 37 TTGTCCT 15 0.0081825955 50.293476 37 ATGCTCG 20 4.043281E-4 50.23281 36 TCCTCAC 15 0.008221796 50.232807 34 CACAATG 15 0.008261136 50.172287 47 CACGATT 15 0.008261136 50.172287 47 >>END_MODULE