FastQCFastQC Report
Fri 17 Jun 2016
SRR1526113_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526113_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3733
Sequences flagged as poor quality0
Sequence length40
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC170.45539780337530134No Hit
GCAGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC160.42860969729440135No Hit
GCAGGAGGGATGAGATGGACTTAAATGAATTGACGGGTAC160.42860969729440135No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC140.3750334851326011No Hit
GCAGGAGGGATGAGATGGACTCAAATGAATTGACGGGTAC120.32145727297080096No Hit
GCAGGAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC110.29466916688990086No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC110.29466916688990086No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG80.21430484864720067No Hit
GCAGGAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC80.21430484864720067No Hit
GCAGGAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC80.21430484864720067No Hit
GCAGGAGGGAATGAAATGCAAGTTACTAAGCGTGAAGAGC80.21430484864720067No Hit
GCAGGAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTT60.16072863648540048No Hit
GCAGGAGGGGAGTCGACTCTTTTTTTTTTTTTTTTTTTTT60.16072863648540048No Hit
GCAGGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT60.16072863648540048No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG60.16072863648540048No Hit
GCAGGAGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGAGC60.16072863648540048No Hit
GCAGGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT60.16072863648540048No Hit
GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT60.16072863648540048No Hit
GCAGGAGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC60.16072863648540048No Hit
GCAGGAGGGGGGGCTGGTCAGATGGCTCCGTGGGTCAGAG50.13394053040450038No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG50.13394053040450038No Hit
GCAGGAGGGATGAGATGGACTCACAGGAATTGACGGGTAC40.10715242432360034No Hit
GCAGGAGGGAGCCGAGTCGATTTTTTTTTTTTTTTTTTTT40.10715242432360034No Hit
GCAGGAGGGTGTCAAGCGATTTTTTTTTTTTTTTTTTTTT40.10715242432360034No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC40.10715242432360034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG405.1184543E-833.9054076
AGGGGAT200.002480232633.9054076
GGATGAG200.002480232633.9054078
AGGGCTC200.002480232633.9054076
GACGGGT200.002480232633.90540732
AGGGATG301.146206E-533.9054076
AGGGAAG200.002480232633.9054076
ACGGGTA200.002480232633.90540733
TTGACGG200.002480232633.90540730
GGGATGA200.002480232633.9054077
GGAGGGA1400.033.9054074
GAGGGGT200.002480232633.9054075
GAGGGGA405.1184543E-833.9054075
GAGGGAT453.3687684E-933.9054075
GAGGGAG405.1184543E-833.9054075
GAGGGAA405.1184543E-833.9054075
TGACGGG200.002480232633.90540731
CGGGTAC251.6926572E-433.90540334
GGAGGGT251.6926572E-433.9054034
GAGGGGC251.6926572E-433.9054035
Produced by FastQC (version 0.11.5)