##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526113_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3733
Sequences flagged as poor quality	0
Sequence length	40
%GC	54
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.925261184034287	29.0	22.0	31.0	4.0	33.0
2	11.228770425930886	11.0	10.0	11.0	4.0	17.0
3	18.226359496383605	19.0	12.0	25.0	4.0	29.0
4	20.798553442271633	23.0	14.0	29.0	4.0	31.0
5	21.40825073667292	24.0	15.0	29.0	4.0	32.0
6	20.797749799089203	23.0	14.0	29.0	4.0	33.0
7	21.165550495579964	24.0	12.0	29.0	4.0	33.0
8	21.363782480578625	25.0	13.0	29.0	4.0	33.0
9	18.498526654165552	22.0	11.0	25.0	4.0	29.0
10	16.028127511384945	16.0	9.0	22.0	4.0	27.0
11	22.653361907313155	26.0	13.0	33.0	4.0	36.0
12	22.875435306723816	26.0	13.0	33.0	4.0	36.0
13	24.09375837128315	28.0	14.0	34.0	4.0	36.0
14	24.601660862577017	30.0	15.0	35.0	4.0	37.0
15	22.800964371818914	27.0	11.0	33.0	4.0	36.0
16	21.39190999196357	25.0	4.0	33.0	4.0	36.0
17	21.778194481650146	26.0	4.0	33.0	4.0	36.0
18	21.395124564693276	26.0	4.0	33.0	4.0	36.0
19	18.22769890168765	18.0	4.0	30.0	4.0	34.0
20	20.057326547013126	23.0	4.0	32.0	4.0	36.0
21	17.687382802035895	17.0	4.0	30.0	4.0	35.0
22	17.322528797214037	17.0	4.0	30.0	4.0	35.0
23	17.610233056522905	18.0	4.0	30.0	4.0	35.0
24	16.978033753013662	15.0	4.0	30.0	4.0	35.0
25	16.604339673185105	14.0	4.0	30.0	4.0	35.0
26	14.528797214036967	4.0	4.0	28.0	4.0	34.0
27	14.308866863112778	4.0	4.0	28.0	4.0	34.0
28	13.79882132333244	4.0	4.0	26.0	4.0	33.0
29	13.551031342084114	4.0	4.0	26.0	4.0	33.0
30	12.974283418162337	4.0	4.0	25.0	4.0	33.0
31	12.459951781409055	4.0	4.0	25.0	4.0	33.0
32	11.988213233324403	4.0	4.0	23.0	4.0	32.0
33	11.33806589874096	4.0	4.0	20.0	4.0	31.0
34	11.056522903830698	4.0	4.0	18.0	4.0	31.0
35	10.26814894186981	4.0	4.0	15.0	4.0	30.0
36	10.135279935708546	4.0	4.0	13.0	4.0	32.0
37	9.977497990892044	4.0	4.0	12.0	4.0	32.0
38	9.886954192338601	4.0	4.0	9.0	4.0	31.0
39	9.58424859362443	4.0	4.0	4.0	4.0	31.0
40	9.375033485132601	4.0	4.0	4.0	4.0	31.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	5.7072470739137415
1	2	19.4426126342793
1	3	-0.21479477312810502
1	4	9.961107102773767
1	5	9.200957992624662
1	6	8.240945165945163
1	7	6.420530703864035
1	8	1.5506774090107385
1	9	4.310990860990859
1	10	5.155379188712521
1	11	-3.379661696328366
1	12	-1.0420113836780516
1	13	-5.988969055635721
1	14	-0.7752886002886044
1	15	-3.3929252845919535
1	16	5.583168991502326
1	17	4.272574955908286
1	18	5.06025733525734
1	19	2.2007094757094734
1	20	6.453719737053067
1	21	6.865480198813529
1	22	6.981862273528938
1	23	7.675673400673407
1	24	7.156441398108067
1	25	11.553928170594839
1	26	7.286079044412384
1	27	9.866951258617926
1	28	10.633028699695371
1	29	6.865901074234403
1	30	7.704910213243549
1	31	5.084118967452296
1	32	4.257178932178933
1	33	5.514526214526212
1	34	1.6377665544332185
1	35	1.6188472021805325
1	36	1.595362353695684
1	37	4.838139329805996
1	38	-3.4063892897226253
1	39	2.860521885521882
1	40	-0.8796256212922895
6	1	-6.149895783229116
6	2	-3.7002445085778435
6	3	-9.214794773128103
6	4	-9.610321468654805
6	5	-9.37047057880391
6	6	-9.330483405483408
6	7	-13.150897867564538
6	8	-12.306465448132117
6	9	-8.831866281866285
6	10	-6.701763668430337
6	11	-4.5225188391855085
6	12	-6.6134399551066245
6	13	-5.703254769921436
6	14	-7.775288600288603
6	15	-9.392925284591952
6	16	-6.845402437069103
6	17	-7.727425044091715
6	18	-6.654028379028375
6	19	-4.085004810004813
6	20	-8.689137405804075
6	21	-9.991662658329329
6	22	-5.303852012185349
6	23	-7.181469456469452
6	24	-8.986415744749074
6	25	-7.731786115119447
6	26	-3.713920955587616
6	27	-3.1330487413820745
6	28	-4.36697130030463
6	29	-1.1340989257655991
6	30	-1.5808040724707375
6	31	-5.20159531826199
6	32	-3.0285353535353536
6	33	-4.62833092833093
6	34	-5.219376302709638
6	35	-2.3811527978194666
6	36	-4.833209074875745
6	37	-4.733289241622575
6	38	-1.4063892897226253
6	39	-2.568049543049546
6	40	-3.736768478435147
7	1	6.564389931056599
7	2	3.585469777136442
7	3	7.928062369729037
7	4	8.818249959916624
7	5	5.200957992624662
7	6	11.383802308802306
7	7	4.70624498957832
7	8	2.8363916947250267
7	9	5.168133718133717
7	10	7.2982363315696634
7	11	6.334624017957349
7	12	4.8151314734648025
7	13	7.582459515792852
7	14	7.224711399711396
7	15	3.3213604296937618
7	16	6.440311848645184
7	17	9.12971781305114
7	18	8.63168590668591
7	19	6.343566618566616
7	20	10.453719737053067
7	21	6.294051627384956
7	22	11.12471941638608
7	23	15.961387686387692
7	24	9.156441398108067
7	25	12.553928170594839
7	26	13.571793330126669
7	27	11.866951258617926
7	28	14.20445727112394
7	29	14.865901074234403
7	30	13.704910213243549
7	31	1.0841189674522944
7	32	-1.3142496392496383
7	33	1.657383357383356
7	34	1.923480840147505
7	35	0.04741863075196129
7	36	4.595362353695684
7	37	2.409567901234567
7	38	3.165039281705946
7	39	3.431950456950453
7	40	2.1203743787077105
8	1	-3.1181497514830845
8	2	-1.1129429212762556
8	3	-4.722731281064611
8	4	-2.9595278178611544
8	5	-3.592692801026132
8	6	-0.12413419913420043
8	7	-4.18264389931057
8	8	-4.766782908449578
8	9	-2.5144059644059666
8	10	-5.003350970017639
8	11	-2.3320426487093187
8	12	-3.835662177328846
8	13	-4.100080166746832
8	14	-3.394336219336221
8	15	-4.5675284591951275
8	16	-1.5596881513548162
8	17	-7.6321869488536205
8	18	-0.9556156806156757
8	19	-1.069131794131799
8	20	-3.1018358185024884
8	21	-2.0710277376944077
8	22	1.5215448132114755
8	23	-0.7846440596440551
8	24	0.6485048901715604
8	25	-5.366706750040082
8	26	1.540047298380637
8	27	-3.275905884239217
8	28	-0.5257014590347886
8	29	-2.2610830527497257
8	30	-0.29508978675645103
8	31	-2.296833413500085
8	32	-2.2825036075036067
8	33	-0.9616642616642643
8	34	-2.060646143979479
8	35	-0.8732162898829596
8	36	-2.5157487574154267
8	37	-2.193606701940036
8	38	-1.7238496071829434
8	39	0.28909331409331074
8	40	0.580691839025171
9	1	8.617961359628026
9	2	6.5676126342793
9	3	8.24949094115761
9	4	8.068249959916624
9	5	9.50452942119609
9	6	3.473088023088021
9	7	11.08124498957832
9	8	7.997105980439311
9	9	10.221705146705144
9	10	9.066093474426806
9	11	7.834624017957349
9	12	11.136560044893375
9	13	8.636030944364279
9	14	6.313997113997111
9	15	9.446360429693762
9	16	2.940311848645184
9	17	8.576146384479713
9	18	9.238828763828767
9	19	3.486423761423758
9	20	7.328719737053067
9	21	12.651194484527814
9	22	6.785433702100365
9	23	8.354244829244834
9	24	8.245727112393782
9	25	9.786071027737696
9	26	5.053936187269526
9	27	5.724094115760783
9	28	5.793742985409656
9	29	-2.2055274971941703
9	30	-0.17008978675645103
9	31	-0.6301667468334191
9	32	2.453607503607504
9	33	-1.3783309283309304
9	34	1.6199094115760762
9	35	-1.470438512105181
9	36	0.845362353695684
9	37	0.9452821869488535
9	38	-1.4599607182940542
9	39	-0.9609066859066893
9	40	-0.3081970498637183
10	1	-2.0070386403719738
10	2	0.44261263427929975
10	3	5.49949094115761
10	4	-10.181750040083376
10	5	6.629529421196089
10	6	0.098088023088021
10	7	1.7062449895783196
10	8	5.122105980439311
10	9	0.5967051467051441
10	10	1.4410934744268058
10	11	7.334624017957349
10	12	13.386560044893375
10	13	12.011030944364279
10	14	11.938997113997111
10	15	4.321360429693762
10	16	12.440311848645184
10	17	11.701146384479713
10	18	12.488828763828767
10	19	14.486423761423758
10	20	16.453719737053067
10	21	19.151194484527814
10	22	21.410433702100363
10	23	18.104244829244834
10	24	21.87072711239378
10	25	21.411071027737698
10	26	12.428936187269526
10	27	16.724094115760785
10	28	-9.081257014590344
10	29	-8.70552749719417
10	30	-8.295089786756451
10	31	-7.630166746833419
10	32	-7.171392496392496
10	33	-6.62833092833093
10	34	-6.505090588423924
10	35	-6.095438512105181
10	36	-6.404637646304316
10	37	-6.3047178130511465
10	38	-5.834960718294054
10	39	-5.710906685906689
10	40	-5.308197049863718
11	1	6.992961359628026
11	2	6.5676126342793
11	3	6.87449094115761
11	4	5.568249959916624
11	5	7.254529421196089
11	6	7.848088023088021
11	7	8.45624498957832
11	8	11.622105980439311
11	9	7.096705146705144
11	10	9.441093474426806
11	11	9.45962401795735
11	12	8.886560044893375
11	13	9.011030944364279
11	14	8.188997113997111
11	15	9.821360429693762
11	16	15.315311848645184
11	17	13.201146384479713
11	18	12.238828763828767
11	19	7.111423761423758
11	20	13.453719737053067
11	21	17.526194484527814
11	22	9.910433702100365
11	23	16.604244829244834
11	24	16.24572711239378
11	25	14.411071027737696
11	26	4.178936187269526
11	27	-0.6509058842392168
11	28	3.293742985409656
11	29	0.7944725028058297
11	30	4.579910213243549
11	31	2.869833253166581
11	32	3.328607503607504
11	33	1.2466690716690696
11	34	5.119909411576076
11	35	-2.720438512105181
11	36	-0.02963764630431598
11	37	-1.4297178130511465
11	38	1.9150392817059458
11	39	-0.46090668590668926
11	40	3.0668029501362817
12	1	-1.5784672118005467
12	2	0.7283269199935862
12	3	0.785205226871895
12	4	2.9611071027737665
12	5	2.3438151354818046
12	6	-0.044769119769121346
12	7	-3.0080407247073957
12	8	5.264963123296454
12	9	1.7395622895622864
12	10	2.0125220458553787
12	11	4.334624017957349
12	12	10.815131473464806
12	13	3.011030944364279
12	14	5.510425685425684
12	15	4.178503286836619
12	16	10.297454705788041
12	17	7.272574955908286
12	18	10.774543049543052
12	19	11.772138047138043
12	20	6.882291165624494
12	21	16.722623055956383
12	22	15.839005130671792
12	23	9.675673400673407
12	24	14.299298540965209
12	25	16.83964245630913
12	26	13.428936187269526
12	27	14.724094115760783
12	28	14.347314413981083
12	29	16.8659010742344
12	30	12.562053070386407
12	31	6.369833253166581
12	32	7.971464646464646
12	33	1.0859547859547831
12	34	7.2091951258617915
12	35	9.190275773609104
12	36	-1.404637646304316
12	37	1.6952821869488535
12	38	-4.549246432579769
12	39	-1.8537638287638325
12	40	-5.308197049863718
13	1	1.9929613596280262
13	2	1.0676126342792998
13	3	3.7494909411576103
13	4	5.693249959916624
13	5	3.5045294211960893
13	6	3.723088023088021
13	7	1.7062449895783196
13	8	3.3721059804393114
13	9	2.596705146705144
13	10	1.0660934744268058
13	11	13.70962401795735
13	12	11.136560044893375
13	13	10.511030944364279
13	14	7.813997113997111
13	15	8.946360429693762
13	16	6.065311848645184
13	17	8.576146384479713
13	18	10.863828763828767
13	19	8.361423761423758
13	20	10.078719737053067
13	21	11.526194484527814
13	22	6.160433702100365
13	23	11.104244829244834
13	24	5.245727112393782
13	25	11.411071027737696
13	26	6.428936187269526
13	27	8.224094115760783
13	28	4.168742985409656
13	29	5.16947250280583
13	30	6.704910213243549
13	31	2.619833253166581
13	32	5.203607503607504
13	33	-0.3783309283309304
13	34	3.244909411576076
13	35	5.029561487894819
13	36	0.345362353695684
13	37	3.4452821869488535
13	38	2.415039281705946
13	39	3.6640933140933107
13	40	4.441802950136282
15	1	6.492961359628026
15	2	1.6926126342792998
15	3	5.74949094115761
15	4	6.568249959916624
15	5	8.37952942119609
15	6	8.598088023088021
15	7	7.45624498957832
15	8	8.247105980439311
15	9	5.971705146705144
15	10	11.816093474426806
15	11	9.33462401795735
15	12	11.011560044893375
15	13	8.261030944364279
15	14	9.063997113997111
15	15	12.571360429693762
15	16	9.565311848645184
15	17	11.951146384479713
15	18	2.238828763828767
15	19	11.611423761423758
15	20	9.203719737053067
15	21	11.651194484527814
15	22	6.660433702100365
15	23	13.854244829244834
15	24	15.120727112393782
15	25	4.411071027737696
15	26	12.303936187269526
15	27	4.224094115760783
15	28	9.043742985409656
15	29	8.16947250280583
15	30	8.579910213243549
15	31	5.119833253166581
15	32	5.203607503607504
15	33	4.62166907166907
15	34	5.494909411576076
15	35	1.654561487894819
15	36	1.220362353695684
15	37	1.8202821869488535
15	38	-2.584960718294054
15	39	-2.4609066859066893
15	40	2.3168029501362817
16	1	4.548516915183583
16	2	3.4426126342792998
16	3	3.8328242744909424
16	4	1.7071388488055135
16	5	7.9628627545294215
16	6	1.9869769119769103
16	7	9.484022767356098
16	8	9.233217091550422
16	9	8.707816257816255
16	10	9.441093474426806
16	11	2.8901795735129063
16	12	5.164337822671154
16	13	3.3443642776976112
16	14	2.938997113997111
16	15	5.765804874138205
16	16	7.329200737534073
16	17	6.923368606701935
16	18	10.266606541606546
16	19	9.375312650312647
16	20	5.231497514830846
16	21	7.595638928972257
16	22	4.410433702100365
16	23	4.993133718133723
16	24	10.53739377906045
16	25	10.299959916626586
16	26	0.540047298380637
16	27	2.5018718935385618
16	28	-0.19236812570145467
16	29	0.7389169472502743
16	30	0.2604657687991043
16	31	-4.74127785794453
16	32	-4.393614718614718
16	33	-4.961664261664263
16	34	-3.171757255090591
16	35	-6.095438512105181
16	36	-6.404637646304316
16	37	-6.3047178130511465
16	38	-5.834960718294054
16	39	-5.710906685906689
16	40	-5.308197049863718
17	1	6.135818502485169
17	2	3.1568983485650133
17	3	3.785205226871895
17	4	3.675392817059482
17	5	6.486672278338947
17	6	6.955230880230879
17	7	6.420530703864035
17	8	9.407820266153596
17	9	3.168133718133717
17	10	9.298236331569663
17	11	0.9060525893859221
17	12	6.957988616321948
17	13	6.439602372935706
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110	17	-9.298853615520287
110	18	-0.5111712361712328
110	19	-7.013576238576242
110	20	-1.546280262946933
110	21	2.6511944845278137
110	22	0.9104337021003648
110	23	-0.39575517075516586
110	24	0.37072711239378187
110	25	1.4110710277376963
110	26	-3.0710638127304737
110	27	6.224094115760783
110	28	6.918742985409656
110	29	7.29447250280583
110	30	7.204910213243549
110	31	6.369833253166581
110	32	6.828607503607504
110	33	-1.1283309283309304
110	34	7.494909411576076
110	35	7.904561487894819
110	36	0.09536235369568402
110	37	0.19528218694885346
110	38	0.6650392817059458
110	39	0.7890933140933107
110	40	1.1918029501362817
111	1	0.9929613596280262
111	2	3.1926126342792998
111	3	-5.090588423897202E-4
111	4	2.568249959916624
111	5	4.629529421196089
111	6	-1.651911976911979
111	7	-3.7937550104216804
111	8	-2.3778940195606886
111	9	-2.903294853294856
111	10	1.9410934744268058
111	11	-4.165375982042651
111	12	-2.6134399551066245
111	13	-1.488969055635721
111	14	-0.5610028860028891
111	15	-0.17863957030623823
111	16	2.690311848645184
111	17	0.7011463844797134
111	18	-1.2611712361712328
111	19	2.486423761423758
111	20	-5.546280262946933
111	21	-0.8488055154721863
111	22	-1.5895662978996352
111	23	-2.145755170755166
111	24	-3.879272887606218
111	25	-1.0889289722623037
111	26	-1.8210638127304737
111	27	-2.275905884239217
111	28	-2.081257014590344
111	29	-2.2055274971941703
111	30	-0.29508978675645103
111	31	-4.630166746833419
111	32	-5.671392496392496
111	33	-2.1283309283309304
111	34	-3.505090588423924
111	35	-1.345438512105181
111	36	1.595362353695684
111	37	1.6952821869488535
111	38	1.1650392817059458
111	39	1.2890933140933107
111	40	1.6918029501362817
112	1	6.742961359628026
112	2	20.4426126342793
112	3	5.99949094115761
112	4	0.8182499599166242
112	5	-4.870470578803911
112	6	3.348088023088021
112	7	-2.2937550104216804
112	8	5.622105980439311
112	9	-5.403294853294856
112	10	0.4410934744268058
112	11	-6.665375982042651
112	12	-12.363439955106625
112	13	-10.738969055635721
112	14	-6.311002886002889
112	15	-10.678639570306238
112	16	-12.309688151354816
112	17	-10.298853615520287
112	18	-10.761171236171233
112	19	-11.763576238576242
112	20	-9.796280262946933
112	21	-5.348805515472186
112	22	-9.339566297899635
112	23	-5.395755170755166
112	24	-11.129272887606218
112	25	-11.588928972262304
112	26	-9.571063812730474
112	27	-9.275905884239217
112	28	-9.081257014590344
112	29	-8.70552749719417
112	30	-8.295089786756451
112	31	-7.630166746833419
112	32	-7.171392496392496
112	33	-6.62833092833093
112	34	-6.505090588423924
112	35	-6.095438512105181
112	36	-6.404637646304316
112	37	-6.3047178130511465
112	38	-5.834960718294054
112	39	-5.710906685906689
112	40	-5.308197049863718
114	1	8.992961359628026
114	2	-0.5573873657207002
114	3	10.49949094115761
114	4	12.818249959916624
114	5	10.62952942119609
114	6	2.098088023088021
114	7	12.70624498957832
114	8	-15.877894019560689
114	9	-13.403294853294856
114	10	-10.558906525573194
114	11	-17.66537598204265
114	12	-17.613439955106625
114	13	-18.98896905563572
114	14	-19.06100288600289
114	15	-17.67863957030624
114	16	-15.559688151354816
114	17	-16.298853615520287
114	18	-15.511171236171233
114	19	-13.513576238576242
114	20	-14.546280262946933
114	21	-11.848805515472186
114	22	-11.589566297899635
114	23	-11.895755170755166
114	24	-11.129272887606218
114	25	-11.588928972262304
114	26	-9.571063812730474
114	27	-9.275905884239217
114	28	-9.081257014590344
114	29	-8.70552749719417
114	30	-8.295089786756451
114	31	-7.630166746833419
114	32	-7.171392496392496
114	33	-6.62833092833093
114	34	-6.505090588423924
114	35	-6.095438512105181
114	36	-6.404637646304316
114	37	-6.3047178130511465
114	38	-5.834960718294054
114	39	-5.710906685906689
114	40	-5.308197049863718
117	1	5.992961359628026
117	2	12.4426126342793
117	3	1.4994909411576103
117	4	1.8182499599166242
117	5	2.6295294211960893
117	6	2.098088023088021
117	7	3.7062449895783196
117	8	-5.877894019560689
117	9	7.596705146705144
117	10	6.441093474426806
117	11	5.334624017957349
117	12	5.3865600448933755
117	13	4.011030944364279
117	14	9.938997113997111
117	15	-4.678639570306238
117	16	4.440311848645184
117	17	10.701146384479713
117	18	6.488828763828767
117	19	10.486423761423758
117	20	0.453719737053067
117	21	7.151194484527814
117	22	-5.589566297899635
117	23	-3.895755170755166
117	24	6.870727112393782
117	25	12.411071027737696
117	26	9.428936187269526
117	27	-6.275905884239217
117	28	17.918742985409658
117	29	19.29447250280583
117	30	18.70491021324355
117	31	18.369833253166583
117	32	23.828607503607504
117	33	24.371669071669068
117	34	15.494909411576076
117	35	19.904561487894817
117	36	24.595362353695684
117	37	27.69528218694885
117	38	25.165039281705944
117	39	28.28909331409331
117	40	28.69180295013628
118	1	6.492961359628026
118	2	1.4426126342792998
118	3	8.99949094115761
118	4	7.818249959916624
118	5	6.629529421196089
118	6	3.098088023088021
118	7	2.7062449895783196
118	8	12.122105980439311
118	9	8.096705146705144
118	10	1.9410934744268058
118	11	9.83462401795735
118	12	9.386560044893375
118	13	-0.4889690556357209
118	14	7.438997113997111
118	15	12.821360429693762
118	16	-1.0596881513548162
118	17	-8.798853615520287
118	18	-8.011171236171233
118	19	0.4864237614237581
118	20	-0.546280262946933
118	21	1.1511944845278137
118	22	2.910433702100365
118	23	3.104244829244834
118	24	3.870727112393782
118	25	1.4110710277376963
118	26	7.928936187269526
118	27	8.224094115760783
118	28	8.418742985409656
118	29	8.79447250280583
118	30	6.204910213243549
118	31	-7.630166746833419
118	32	-7.171392496392496
118	33	-6.62833092833093
118	34	-6.505090588423924
118	35	-6.095438512105181
118	36	-6.404637646304316
118	37	-6.3047178130511465
118	38	-5.834960718294054
118	39	-5.710906685906689
118	40	-5.308197049863718
119	1	10.992961359628026
119	2	-0.5573873657207002
119	3	13.49949094115761
119	4	14.818249959916624
119	5	12.62952942119609
119	6	-15.901911976911979
119	7	-16.29375501042168
119	8	-15.877894019560689
119	9	-13.403294853294856
119	10	-10.558906525573194
119	11	-17.66537598204265
119	12	-17.613439955106625
119	13	-18.98896905563572
119	14	-19.06100288600289
119	15	-17.67863957030624
119	16	-15.559688151354816
119	17	-16.298853615520287
119	18	-15.511171236171233
119	19	-13.513576238576242
119	20	-14.546280262946933
119	21	-11.848805515472186
119	22	-11.589566297899635
119	23	-11.895755170755166
119	24	-11.129272887606218
119	25	-11.588928972262304
119	26	-9.571063812730474
119	27	-9.275905884239217
119	28	-9.081257014590344
119	29	-8.70552749719417
119	30	-8.295089786756451
119	31	-7.630166746833419
119	32	-7.171392496392496
119	33	-6.62833092833093
119	34	-6.505090588423924
119	35	-6.095438512105181
119	36	-6.404637646304316
119	37	-6.3047178130511465
119	38	-5.834960718294054
119	39	-5.710906685906689
119	40	-5.308197049863718
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	612.0
5	16.0
6	27.0
7	38.0
8	28.0
9	42.0
10	64.0
11	119.0
12	124.0
13	127.0
14	153.0
15	174.0
16	177.0
17	176.0
18	182.0
19	190.0
20	182.0
21	183.0
22	172.0
23	197.0
24	169.0
25	176.0
26	137.0
27	111.0
28	61.0
29	42.0
30	27.0
31	18.0
32	5.0
33	1.0
34	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	99.3293991416309	0.26824034334763946	0.13412017167381973	0.26824034334763946
8	98.09753483386923	0.669882100750268	0.40192926045016075	0.8306538049303323
9	98.63123993558777	0.7783145464304885	0.322061191626409	0.2683843263553409
10	47.30779533886954	34.5030806321993	8.277524778998124	9.91159924993303
11	34.2887757835521	26.35949638360568	25.475488882935977	13.876238949906242
12	44.81650147334583	17.01044736137155	18.858826680953655	19.314224484328957
13	27.00241092954728	25.100455397803373	22.71631395660327	25.180819716046077
14	27.672113581569786	20.358960621484062	29.225823734261986	22.743102062684166
15	26.814894186980982	28.368604339673187	24.484328957942676	20.332172515403162
16	29.581993569131832	18.783494105037512	30.466237942122188	21.168274383708468
17	35.25314760246451	20.841146530940264	23.14492365389767	20.760782212697563
18	35.89606214840611	20.332172515403162	21.618001607286367	22.153763728904366
19	21.836295099623047	20.786214324178783	15.347334410339256	42.03015616585891
20	25.93088668631128	24.886150549156174	22.180551834985266	27.00241092954728
21	22.287704259308867	22.71631395660327	31.7706938119475	23.22528797214037
22	20.975087061344762	17.59978569515135	30.859898205196895	30.565229038306992
23	25.127243503884277	24.35038842753817	29.145459416019285	21.376908652558264
24	24.939726761317974	24.270024109295473	26.439860701848378	24.35038842753817
25	23.873390557939913	25.777896995708154	24.919527896995707	25.429184549356222
26	21.470905172413794	17.40301724137931	34.72521551724138	26.40086206896552
27	23.81462630592017	13.68872220733994	35.038842753817306	27.457808732922583
28	15.46916890080429	23.53887399463807	33.056300268096514	27.935656836461124
29	18.025751072961373	22.398068669527895	32.43025751072962	27.145922746781114
30	17.533512064343164	13.431635388739945	45.09383378016086	23.94101876675603
31	17.92326267775691	13.657096860745908	46.95465521867453	21.464985242822646
32	28.904366461291186	11.384945084382535	38.039110634878114	21.671577819448164
33	19.935691318327976	18.19399785637728	42.336548767416936	19.533762057877812
34	15.724618269488348	12.054647736405036	48.379319582105545	23.841414412001072
35	19.640943193997856	16.559485530546624	44.855305466237944	18.94426580921758
36	19.346195069667736	17.926045016077172	42.14898177920686	20.578778135048232
37	22.177527487262	15.821936175918477	42.12925717350496	19.87127916331456
38	14.760246450575945	17.465845164746852	48.272167157781944	19.50174122689526
39	18.461950696677384	22.079314040728832	39.92497320471597	19.533762057877812
40	14.251272435038842	16.01928743637825	40.36967586391642	29.359764264666488
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	2.0
20	4.0
21	6.0
22	8.0
23	8.0
24	11.0
25	14.0
26	27.5
27	41.0
28	41.0
29	48.5
30	56.0
31	72.5
32	89.0
33	89.0
34	98.5
35	108.0
36	114.5
37	121.0
38	121.0
39	142.5
40	164.0
41	174.5
42	185.0
43	185.0
44	203.0
45	221.0
46	226.5
47	232.0
48	232.0
49	261.5
50	291.0
51	285.5
52	280.0
53	280.0
54	257.0
55	234.0
56	229.0
57	224.0
58	224.0
59	218.5
60	213.0
61	229.5
62	246.0
63	246.0
64	242.5
65	239.0
66	248.5
67	258.0
68	258.0
69	236.5
70	215.0
71	175.0
72	135.0
73	135.0
74	112.5
75	90.0
76	62.0
77	34.0
78	34.0
79	27.0
80	20.0
81	13.0
82	6.0
83	6.0
84	5.0
85	4.0
86	2.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.13394053040450038
8	0.026788106080900084
9	0.18751674256630055
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.026788106080900084
17	0.0
18	0.0
19	0.5089740155371015
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.13394053040450038
26	0.5625502276989017
27	0.0
28	0.08036431824270024
29	0.13394053040450038
30	0.08036431824270024
31	0.16072863648540048
32	0.0
33	0.026788106080900084
34	0.0
35	0.026788106080900084
36	0.026788106080900084
37	0.10715242432360034
38	0.0
39	0.026788106080900084
40	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	3733.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.29386552370747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.51056338028168	87.19528529332976
2	3.1983568075117366	5.839807125636217
3	0.5575117370892019	1.5269220466113045
4	0.11737089201877934	0.42860969729440135
5	0.05868544600938967	0.26788106080900076
6	0.2347417840375587	1.2858290918832038
7	0.0	0.0
8	0.11737089201877934	0.8572193945888027
9	0.0	0.0
>10	0.20539906103286387	2.5984462898473075
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCAGGAGGGATGAGATGGCCTTAAAGGAATTGACGGGTAC	17	0.45539780337530134	No Hit
GCAGGAGGGATGAGATGGACTTAAAGGAATTGACGGGTAC	16	0.42860969729440135	No Hit
GCAGGAGGGATGAGATGGACTTAAATGAATTGACGGGTAC	16	0.42860969729440135	No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	14	0.3750334851326011	No Hit
GCAGGAGGGATGAGATGGACTCAAATGAATTGACGGGTAC	12	0.32145727297080096	No Hit
GCAGGAGGGATGAGATGGACTCAAAGGAATTGACGGGTAC	11	0.29466916688990086	No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC	11	0.29466916688990086	No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG	8	0.21430484864720067	No Hit
GCAGGAGGGATGAGATGGCCTTAAATGAATTGACGGGTAC	8	0.21430484864720067	No Hit
GCAGGAGGGATGAGATGGCCTCAAAGGAATTGACGGGTAC	8	0.21430484864720067	No Hit
GCAGGAGGGAATGAAATGCAAGTTACTAAGCGTGAAGAGC	8	0.21430484864720067	No Hit
GCAGGAGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTT	6	0.16072863648540048	No Hit
GCAGGAGGGGAGTCGACTCTTTTTTTTTTTTTTTTTTTTT	6	0.16072863648540048	No Hit
GCAGGAGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT	6	0.16072863648540048	No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG	6	0.16072863648540048	No Hit
GCAGGAGGGGGGCTGGTGCGATGGCTCCGTGGGTAAGAGC	6	0.16072863648540048	No Hit
GCAGGAGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.16072863648540048	No Hit
GCAGGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	6	0.16072863648540048	No Hit
GCAGGAGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC	6	0.16072863648540048	No Hit
GCAGGAGGGGGGGCTGGTCAGATGGCTCCGTGGGTCAGAG	5	0.13394053040450038	No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAG	5	0.13394053040450038	No Hit
GCAGGAGGGATGAGATGGACTCACAGGAATTGACGGGTAC	4	0.10715242432360034	No Hit
GCAGGAGGGAGCCGAGTCGATTTTTTTTTTTTTTTTTTTT	4	0.10715242432360034	No Hit
GCAGGAGGGTGTCAAGCGATTTTTTTTTTTTTTTTTTTTT	4	0.10715242432360034	No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCCGTGGTAGAGCC	4	0.10715242432360034	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGGGG	40	5.1184543E-8	33.905407	6
AGGGGAT	20	0.0024802326	33.905407	6
GGATGAG	20	0.0024802326	33.905407	8
AGGGCTC	20	0.0024802326	33.905407	6
GACGGGT	20	0.0024802326	33.905407	32
AGGGATG	30	1.146206E-5	33.905407	6
AGGGAAG	20	0.0024802326	33.905407	6
ACGGGTA	20	0.0024802326	33.905407	33
TTGACGG	20	0.0024802326	33.905407	30
GGGATGA	20	0.0024802326	33.905407	7
GGAGGGA	140	0.0	33.905407	4
GAGGGGT	20	0.0024802326	33.905407	5
GAGGGGA	40	5.1184543E-8	33.905407	5
GAGGGAT	45	3.3687684E-9	33.905407	5
GAGGGAG	40	5.1184543E-8	33.905407	5
GAGGGAA	40	5.1184543E-8	33.905407	5
TGACGGG	20	0.0024802326	33.905407	31
CGGGTAC	25	1.6926572E-4	33.905403	34
GGAGGGT	25	1.6926572E-4	33.905403	4
GAGGGGC	25	1.6926572E-4	33.905403	5
>>END_MODULE