FastQCFastQC Report
Fri 17 Jun 2016
SRR1526111_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526111_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1489
Sequences flagged as poor quality0
Sequence length40
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT382.552048354600403No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC161.0745466756212223No Hit
GCAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGC90.6044325050369375No Hit
GCAGGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT80.5372733378106112No Hit
GCAGGAGATTGGGCGGATTGAACAAAAACTCATCTCAGAA70.47011417058428473No Hit
GCAGGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG70.47011417058428473No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG40.2686366689053056No Hit
GCAGGAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAG40.2686366689053056No Hit
GCAGGAGGGGTGTTCTTTATTAATATCCTAACACTCCTCG40.2686366689053056No Hit
GCAGGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT30.20147750167897915No Hit
GCAGGAGGGGTGTTTTTTATTAATATCCTAACACTCCTCG30.20147750167897915No Hit
GCAGGAGGGTACATGGTTGATCCTGCCAGTAGCATATGCT30.20147750167897915No Hit
GCAGGAGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAA30.20147750167897915No Hit
GCAGGAGGGTACCTGGTTGCTCCTGCCAGTAGCATATGCT30.20147750167897915No Hit
GCAGGAGGGATGGTATCTATTTATCAGAAAGGCATGTTTC30.20147750167897915No Hit
GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA30.20147750167897915No Hit
GCAGGAGGGGCTCGTTTTCCCTGAAGGTTCTCTGATCTAG20.1343183344526528No Hit
GCAGGAGGGGGTAATGGTCTGTTTACTTCCTGCGGCTGGA20.1343183344526528No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG20.1343183344526528No Hit
GCAGGAGGGGTTTCCCCCTCATAAGTCATCCCCTCAGAGG20.1343183344526528No Hit
GCAGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1343183344526528No Hit
GCAGGAGGGAAGCAGAGGTATCAACGCAGTGTCGACTTTT20.1343183344526528No Hit
GCAGGAGGGGGTTGCCACTGTGCAGGTGTTTAGGGCAGGT20.1343183344526528No Hit
GCAGGAGGGTCACGGATCCTCTTCTGAGATGAGTTTTTGT20.1343183344526528No Hit
GCAGGAGGGATCCCTAACTCTCCTAGACTTGATCTGGTTA20.1343183344526528No Hit
GCAGGAGGGCCTAATCCTAGCCCTAGCCCTACACAAATAT20.1343183344526528No Hit
GCAGGAGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT20.1343183344526528No Hit
GCAGGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC20.1343183344526528No Hit
GCAGGAGGGGCCGGGGGCATTCGTATTGCGCCGCTAGAGG20.1343183344526528No Hit
GCAGGAGGGATGCCACAACTAGATACATCAACATGATTTA20.1343183344526528No Hit
GCAGGAGGGAGTGCATTGCTGCAGGAGGGTCTCTAAACTT20.1343183344526528No Hit
GCAGGAGGGGGCTGAGACAGGAGGACAGTGGCCTTGCTTC20.1343183344526528No Hit
GCAGGAGGGGTTGTTAGCGAGCAAGTTTCAAGCTACTTGT20.1343183344526528No Hit
GCAGGAGGGCTCCTACTTGGATAAATGTGGTAATTCTAGA20.1343183344526528No Hit
GCAGGAGGGGAGGGATCTGATGCGGGCCGGGGTGTTGAAA20.1343183344526528No Hit
GCAGGAGGGAACTAGTCTGGCAGGCGCTCCGTTCAGTGAA20.1343183344526528No Hit
GCAGGAGGGGAGTCTGAGAATGTGAATGGGGTAAGTGCTT20.1343183344526528No Hit
GCAGGAGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTG20.1343183344526528No Hit
GCAGGAGGGAGTCTTTCAAGTCGTGTGCATTGCACGTTGG20.1343183344526528No Hit
GCAGGAGATTGGGAGGATTGAACAAAAACTCATCTCAGAA20.1343183344526528No Hit
GCAGGAGGGGGTAGTTTACGGGACTCCGATCGTCTTGCTG20.1343183344526528No Hit
GCAGGAGGGGCAATAATGTCTATTGGCTTTCTAGGCTTTA20.1343183344526528No Hit
GCAGGAGGGGGAGGAGGAAGAGACTGCTGGTTTTGGGGGA20.1343183344526528No Hit
GCAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1343183344526528No Hit
GCAGGAGGGCAACTTTCGATGGTAGTCGCCGTGCCTACCA20.1343183344526528No Hit
GCAGGAGGGTACCTGGTTGATACTGCCAGTAGCATATGCT20.1343183344526528No Hit
GCAGGAGGGGTTAGCTGACTTGCAACCCTACACGGAGGTA20.1343183344526528No Hit
GCAGGAGGGGGTCTTTGCTAAGGGTCACATTGAAGTAACG20.1343183344526528No Hit
GCAGGAGGGACAGGAAAAGGAGGAGAGCAAGAAGGAGAGA20.1343183344526528No Hit
GCAGGAGGGGTAACTGGTTGATCCTGCCAGTAGCATATGC20.1343183344526528No Hit
GCAGGAGGGACTAATCCTAGCACTAGCCCTACACAAATAT20.1343183344526528No Hit
GCAGGAGGGGTTTCAATGGCTTTCTTCCCTTGACCTTTCC20.1343183344526528No Hit
GCAGGAGGGGGGGAGAATCCGCGTTGAAGAACAGAGCATT20.1343183344526528No Hit
GCAGGAGGGGAGCTACAAGGCTTGCTAGGCATCTGTCTGT20.1343183344526528No Hit
GCAGGAGGGTACCTGGTTGATCCTGCCAGTACCATATGCT20.1343183344526528No Hit
GCAGGAGGGGGATTCGTCTTGACATTGCTCTCTGTGACGA20.1343183344526528No Hit
GCAGGAGGGGCGAATGGCTCATTAAATCAGTTATGGTTCC20.1343183344526528No Hit
GCAGGAGGGATTGTGTTTTAGGACTCATTGGCTGAGTATA20.1343183344526528No Hit
GCAGGAGGGGATTGGGAGAGAGATTGATTCAGATTCGCTC20.1343183344526528No Hit
GCAGGAGGGCGGGGAGGTAGTGACGAAAAATAACAATACA20.1343183344526528No Hit
GCAGGAGGGCCGTGGCTGTAAGTCAGGAGGAGTATGGTGA20.1343183344526528No Hit
GCAGGAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1343183344526528No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAGC20.1343183344526528No Hit
GCAGGAGGGGATTTGTGTGTGTATGTGTGTATATGTGTAT20.1343183344526528No Hit
GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC20.1343183344526528No Hit
GCAGGAGGGAGCCGCATCAATATTGTCACTGCCGATTTTG20.1343183344526528No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGT259.9473706E-534.9642875
GAGGGGG353.0626688E-734.9642875
GAGGGGA259.9473706E-534.9642875
AGGGGGG200.001695275734.9642836
AGGGGAT200.001695275734.9642836
GGAGGGG950.034.9642834
GGGGATT200.001695275734.9642837
GCAGGAG1500.032.6333351
CAGGAGG1350.032.50832
AGGAGGG1350.032.50833
GGAGGGA302.9134535E-429.1369044
Produced by FastQC (version 0.11.5)