##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526111_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1489 Sequences flagged as poor quality 0 Sequence length 40 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 17.82538616521155 22.0 4.0 31.0 4.0 35.0 2 13.584956346541302 4.0 4.0 25.0 4.0 32.0 3 12.523169912693083 4.0 4.0 22.0 4.0 31.0 4 13.139691067830759 4.0 4.0 24.0 4.0 33.0 5 12.29818670248489 4.0 4.0 22.0 4.0 33.0 6 10.48153122901276 4.0 4.0 13.0 4.0 31.0 7 11.55137676292814 4.0 4.0 20.0 4.0 33.0 8 11.691739422431162 4.0 4.0 20.0 4.0 33.0 9 10.503022162525184 4.0 4.0 17.0 4.0 30.0 10 10.122229684351915 4.0 4.0 16.0 4.0 28.0 11 10.625251846877099 4.0 4.0 16.0 4.0 31.0 12 10.76158495634654 4.0 4.0 17.0 4.0 31.0 13 10.443922095366018 4.0 4.0 16.0 4.0 30.0 14 10.645399597044996 4.0 4.0 17.0 4.0 31.0 15 10.732034922766958 4.0 4.0 16.0 4.0 33.0 16 9.044325050369375 4.0 4.0 7.0 4.0 28.0 17 8.642041638683681 4.0 4.0 5.0 4.0 26.0 18 8.383478844862324 4.0 4.0 4.0 4.0 25.0 19 7.981195433176628 4.0 4.0 4.0 4.0 23.0 20 8.076561450638012 4.0 4.0 4.0 4.0 24.0 21 7.878441907320349 4.0 4.0 4.0 4.0 24.0 22 7.507051712558765 4.0 4.0 4.0 4.0 21.0 23 7.6406984553391535 4.0 4.0 4.0 4.0 22.0 24 7.645399597044997 4.0 4.0 4.0 4.0 22.0 25 7.67494963062458 4.0 4.0 4.0 4.0 22.0 26 8.005372733378106 4.0 4.0 4.0 4.0 25.0 27 7.985224983210208 4.0 4.0 4.0 4.0 25.0 28 7.999328408327737 4.0 4.0 4.0 4.0 25.0 29 7.594358629952988 4.0 4.0 4.0 4.0 23.0 30 7.8032236400268635 4.0 4.0 4.0 4.0 24.0 31 8.249832102081934 4.0 4.0 4.0 4.0 29.0 32 8.060443250503694 4.0 4.0 4.0 4.0 27.0 33 8.053055742108798 4.0 4.0 4.0 4.0 27.0 34 8.192746809939557 4.0 4.0 4.0 4.0 28.0 35 7.935527199462727 4.0 4.0 4.0 4.0 27.0 36 7.90329079919409 4.0 4.0 4.0 4.0 28.0 37 7.742108797850906 4.0 4.0 4.0 4.0 27.0 38 7.435862995298859 4.0 4.0 4.0 4.0 24.0 39 7.32505036937542 4.0 4.0 4.0 4.0 24.0 40 7.608462055070517 4.0 4.0 4.0 4.0 28.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 18.89549062049062 1 2 9.985714285714284 1 3 7.830411255411256 1 4 22.07417027417027 1 5 23.647402597402596 1 6 22.44906204906205 1 7 -7.468470418470417 1 8 -8.027597402597403 1 9 -6.515151515151517 1 10 -5.676803751803753 1 11 -6.957539682539684 1 12 -6.338816738816741 1 13 -6.089430014430013 1 14 -6.2484487734487715 1 15 -6.493722943722945 1 16 -3.946212121212122 1 17 -3.8285353535353526 1 18 -3.250793650793651 1 19 -2.9386363636363635 1 20 -2.8694083694083687 1 21 -2.9429653679653676 1 22 -3.2495310245310245 1 23 -3.5185786435786435 1 24 -3.252272727272727 1 25 -3.161038961038961 1 26 -3.7157647907647906 1 27 -3.5275974025974026 1 28 -3.166630591630592 1 29 -3.3978715728715745 1 30 -3.474747474747474 1 31 -3.5008658008658022 1 32 -3.430411255411256 1 33 -3.2540404040404036 1 34 -3.795057720057721 1 35 -3.221572871572871 1 36 -2.830447330447331 1 37 -2.833297258297258 1 38 -2.625937950937951 1 39 -2.3470057720057724 1 40 -2.518867243867244 2 1 6.3954906204906194 2 2 2.735714285714284 2 3 -0.16958874458874362 2 4 -6.675829725829727 2 5 -4.102597402597404 2 6 -5.05093795093795 2 7 -4.468470418470417 2 8 -5.527597402597403 2 9 -6.015151515151517 2 10 -1.176803751803753 2 11 -5.207539682539684 2 12 -2.3388167388167407 2 13 -4.089430014430013 2 14 -1.2484487734487715 2 15 -2.743722943722945 2 16 -0.9462121212121222 2 17 -2.8285353535353526 2 18 1.4992063492063492 2 19 -0.6886363636363635 2 20 -1.3694083694083687 2 21 1.8070346320346324 2 22 -1.7495310245310245 2 23 0.4814213564213565 2 24 -0.752272727272727 2 25 2.588961038961039 2 26 3.0342352092352094 2 27 3.2224025974025974 2 28 -1.6666305916305921 2 29 -0.8978715728715745 2 30 -0.47474747474747403 2 31 3.4991341991341978 2 32 3.819588744588744 2 33 3.7459595959595964 2 34 3.704942279942279 2 35 4.028427128427129 2 36 5.669552669552669 2 37 5.166702741702742 2 38 5.374062049062049 2 39 5.652994227994228 2 40 2.231132756132756 3 1 -13.10450937950938 3 2 -9.014285714285716 3 3 -8.169588744588744 3 4 -8.925829725829727 3 5 -8.352597402597404 3 6 -6.55093795093795 3 7 -7.468470418470417 3 8 -8.027597402597403 3 9 -6.515151515151517 3 10 -5.676803751803753 3 11 -6.957539682539684 3 12 -6.338816738816741 3 13 -6.089430014430013 3 14 -6.2484487734487715 3 15 -6.493722943722945 3 16 -3.946212121212122 3 17 -3.8285353535353526 3 18 -3.250793650793651 3 19 -2.9386363636363635 3 20 -2.8694083694083687 3 21 -2.9429653679653676 3 22 -3.2495310245310245 3 23 -3.5185786435786435 3 24 -3.252272727272727 3 25 -3.161038961038961 3 26 -3.7157647907647906 3 27 -3.5275974025974026 3 28 -3.166630591630592 3 29 -3.3978715728715745 3 30 -3.474747474747474 3 31 -3.5008658008658022 3 32 -3.430411255411256 3 33 -3.2540404040404036 3 34 -3.795057720057721 3 35 -3.221572871572871 3 36 -2.830447330447331 3 37 -2.833297258297258 3 38 -2.625937950937951 3 39 -2.3470057720057724 3 40 -2.518867243867244 5 1 -4.1045093795093806 5 2 -0.514285714285716 5 3 -6.169588744588744 5 4 -2.9258297258297272 5 5 2.1474025974025963 5 6 -3.05093795093795 5 7 -5.968470418470417 5 8 -6.527597402597403 5 9 -4.015151515151517 5 10 0.8231962481962469 5 11 -0.957539682539684 5 12 -2.8388167388167407 5 13 -4.089430014430013 5 14 -2.7484487734487715 5 15 2.506277056277055 5 16 9.053787878787878 5 17 0.17146464646464743 5 18 10.24920634920635 5 19 0.06136363636363651 5 20 0.13059163059163126 5 21 6.057034632034632 5 22 7.2504689754689755 5 23 5.4814213564213565 5 24 2.747727272727273 5 25 4.338961038961039 5 26 9.28423520923521 5 27 11.972402597402597 5 28 10.333369408369407 5 29 9.602128427128426 5 30 12.525252525252526 5 31 0.49913419913419776 5 32 7.569588744588744 5 33 0.24595959595959638 5 34 10.70494227994228 5 35 9.77842712842713 5 36 2.169552669552669 5 37 7.666702741702742 5 38 9.374062049062049 5 39 0.6529942279942276 5 40 10.481132756132755 8 1 2.3954906204906194 8 2 6.485714285714284 8 3 7.330411255411256 8 4 6.574170274170273 8 5 7.147402597402596 8 6 -1.05093795093795 8 7 4.531529581529583 8 8 5.472402597402597 8 9 -6.515151515151517 8 10 -5.676803751803753 8 11 -6.957539682539684 8 12 -6.338816738816741 8 13 -6.089430014430013 8 14 -6.2484487734487715 8 15 -6.493722943722945 8 16 -3.946212121212122 8 17 -3.8285353535353526 8 18 -3.250793650793651 8 19 -2.9386363636363635 8 20 -2.8694083694083687 8 21 -2.9429653679653676 8 22 -3.2495310245310245 8 23 -3.5185786435786435 8 24 -3.252272727272727 8 25 -3.161038961038961 8 26 -3.7157647907647906 8 27 -3.5275974025974026 8 28 -3.166630591630592 8 29 -3.3978715728715745 8 30 -3.474747474747474 8 31 -3.5008658008658022 8 32 -3.430411255411256 8 33 -3.2540404040404036 8 34 -3.795057720057721 8 35 -3.221572871572871 8 36 -2.830447330447331 8 37 -2.833297258297258 8 38 -2.625937950937951 8 39 -2.3470057720057724 8 40 -2.518867243867244 9 1 1.5621572871572873 9 2 -2.180952380952382 9 3 -0.0029220779220775484 9 4 0.5741702741702728 9 5 -1.3525974025974037 9 6 -4.55093795093795 9 7 -3.6351370851370843 9 8 -5.860930735930736 9 9 -5.015151515151517 9 10 -4.176803751803753 9 11 -1.790873015873018 9 12 -3.3388167388167407 9 13 -3.089430014430013 9 14 -1.5817821067821054 9 15 -3.493722943722945 9 16 -2.612878787878789 9 17 -2.6618686868686856 9 18 1.0825396825396831 9 19 1.3946969696969704 9 20 -1.5360750360750357 9 21 -0.9429653679653676 9 22 -1.2495310245310245 9 23 1.3147546897546905 9 24 0.41439393939394 9 25 -1.1610389610389609 9 26 -0.2157647907647906 9 27 -2.5275974025974026 9 28 -1.6666305916305921 9 29 -2.0645382395382414 9 30 1.191919191919192 9 31 1.3324675324675317 9 32 0.569588744588744 9 33 1.2459595959595964 9 34 -0.2950577200577209 9 35 0.9450937950937952 9 36 2.0028860028860027 9 37 2.333369408369408 9 38 0.540728715728716 9 39 -0.18033910533910547 9 40 2.647799422799422 10 1 11.89549062049062 10 2 -3.014285714285716 10 3 6.830411255411256 10 4 4.074170274170273 10 5 -4.352597402597404 10 6 5.44906204906205 10 7 7.531529581529583 10 8 7.972402597402597 10 9 14.484848484848483 10 10 10.323196248196247 10 11 5.042460317460316 10 12 6.661183261183259 10 13 8.910569985569987 10 14 -0.24844877344877148 10 15 16.506277056277057 10 16 2.053787878787878 10 17 15.171464646464647 10 18 15.74920634920635 10 19 2.0613636363636365 10 20 7.130591630591631 10 21 21.057034632034632 10 22 15.750468975468976 10 23 20.481421356421357 10 24 20.747727272727275 10 25 5.838961038961039 10 26 23.28423520923521 10 27 17.4724025974026 10 28 21.833369408369407 10 29 11.602128427128426 10 30 7.525252525252526 10 31 22.499134199134197 10 32 17.569588744588742 10 33 19.745959595959597 10 34 19.20494227994228 10 35 25.77842712842713 10 36 31.16955266955267 10 37 31.166702741702743 10 38 31.374062049062047 10 39 31.652994227994228 10 40 25.481132756132755 13 1 -13.10450937950938 13 2 -9.014285714285716 13 3 -8.169588744588744 13 4 -8.925829725829727 13 5 -8.352597402597404 13 6 -6.55093795093795 13 7 -7.468470418470417 13 8 -8.027597402597403 13 9 -6.515151515151517 13 10 -5.676803751803753 13 11 -6.957539682539684 13 12 -6.338816738816741 13 13 -6.089430014430013 13 14 -6.2484487734487715 13 15 -6.493722943722945 13 16 -3.946212121212122 13 17 -3.8285353535353526 13 18 -3.250793650793651 13 19 -2.9386363636363635 13 20 -2.8694083694083687 13 21 -2.9429653679653676 13 22 -3.2495310245310245 13 23 -3.5185786435786435 13 24 -3.252272727272727 13 25 -3.161038961038961 13 26 -3.7157647907647906 13 27 -3.5275974025974026 13 28 -3.166630591630592 13 29 -3.3978715728715745 13 30 -3.474747474747474 13 31 -3.5008658008658022 13 32 -3.430411255411256 13 33 -3.2540404040404036 13 34 -3.795057720057721 13 35 -3.221572871572871 13 36 -2.830447330447331 13 37 -2.833297258297258 13 38 -2.625937950937951 13 39 -2.3470057720057724 13 40 -2.518867243867244 16 1 14.69549062049062 16 2 8.985714285714284 16 3 19.630411255411257 16 4 20.674170274170272 16 5 5.047402597402595 16 6 20.64906204906205 16 7 4.131529581529582 16 8 16.5724025974026 16 9 11.484848484848483 16 10 7.923196248196248 16 11 5.842460317460317 16 12 5.46118326118326 16 13 6.910569985569987 16 14 8.951551226551228 16 15 9.906277056277053 16 16 7.6537878787878775 16 17 3.771464646464647 16 18 8.74920634920635 16 19 8.461363636363636 16 20 6.930591630591632 16 21 10.857034632034633 16 22 8.950468975468976 16 23 5.281421356421357 16 24 6.347727272727273 16 25 1.6389610389610398 16 26 6.08423520923521 16 27 5.672402597402597 16 28 1.2333694083694082 16 29 3.002128427128426 16 30 0.3252525252525258 16 31 2.899134199134198 16 32 0.7695887445887433 16 33 8.145959595959596 16 34 6.404942279942278 16 35 7.1784271284271295 16 36 8.169552669552669 16 37 3.366702741702741 16 38 7.17406204906205 16 39 6.052994227994228 16 40 4.881132756132756 18 1 8.89549062049062 18 2 3.7857142857142847 18 3 5.030411255411256 18 4 12.474170274170271 18 5 13.847402597402596 18 6 13.44906204906205 18 7 13.931529581529581 18 8 12.572402597402599 18 9 12.284848484848483 18 10 13.123196248196248 18 11 11.442460317460315 18 12 10.861183261183259 18 13 9.710569985569988 18 14 5.7515512265512285 18 15 11.706277056277054 18 16 11.853787878787879 18 17 7.171464646464647 18 18 9.74920634920635 18 19 8.461363636363636 18 20 9.33059163059163 18 21 10.857034632034633 18 22 12.550468975468977 18 23 10.481421356421357 18 24 7.347727272727273 18 25 10.038961038961038 18 26 11.08423520923521 18 27 13.472402597402597 18 28 13.833369408369407 18 29 14.602128427128426 18 30 13.525252525252526 18 31 16.299134199134198 18 32 16.169588744588744 18 33 16.945959595959597 18 34 17.20494227994228 18 35 19.97842712842713 18 36 12.169552669552669 18 37 11.166702741702743 18 38 13.374062049062049 18 39 11.452994227994228 18 40 14.481132756132755 19 1 18.609776334776335 19 2 20.12857142857143 19 3 19.973268398268402 19 4 22.64559884559884 19 5 20.218831168831166 19 6 16.877633477633477 19 7 22.817243867243867 19 8 22.54383116883117 19 9 22.484848484848484 19 10 21.60891053391053 19 11 16.756746031746033 19 12 22.08975468975469 19 13 18.19628427128427 19 14 20.465836940836944 19 15 20.79199134199134 19 16 20.05378787878788 19 17 19.17146464646465 19 18 19.034920634920635 19 19 11.061363636363637 19 20 7.844877344877345 19 21 13.342748917748917 19 22 11.036183261183261 19 23 13.6242784992785 19 24 13.747727272727273 19 25 11.267532467532465 19 26 15.141378066378067 19 27 18.04383116883117 19 28 12.547655122655122 19 29 10.744985569985568 19 30 15.525252525252526 19 31 11.356277056277055 19 32 10.426731601731602 19 33 12.745959595959597 19 34 12.062085137085138 19 35 7.635569985569987 19 36 9.312409812409811 19 37 5.880988455988455 19 38 3.802633477633478 19 39 5.938708513708514 19 40 2.909704184704185 20 1 -7.6045093795093806 20 2 -6.264285714285716 20 3 -7.169588744588744 20 4 -2.4258297258297272 20 5 -8.352597402597404 20 6 -6.55093795093795 20 7 -7.468470418470417 20 8 -8.027597402597403 20 9 -6.515151515151517 20 10 -5.676803751803753 20 11 -6.957539682539684 20 12 -6.338816738816741 20 13 -6.089430014430013 20 14 -6.2484487734487715 20 15 -6.493722943722945 20 16 -3.946212121212122 20 17 -3.8285353535353526 20 18 -3.250793650793651 20 19 -2.9386363636363635 20 20 -2.8694083694083687 20 21 -2.9429653679653676 20 22 -3.2495310245310245 20 23 -3.5185786435786435 20 24 -3.252272727272727 20 25 -3.161038961038961 20 26 -3.7157647907647906 20 27 -3.5275974025974026 20 28 -3.166630591630592 20 29 -3.3978715728715745 20 30 -3.474747474747474 20 31 -3.5008658008658022 20 32 -3.430411255411256 20 33 -3.2540404040404036 20 34 -3.795057720057721 20 35 -3.221572871572871 20 36 -2.830447330447331 20 37 -2.833297258297258 20 38 -2.625937950937951 20 39 -2.3470057720057724 20 40 -2.518867243867244 24 1 -13.10450937950938 24 2 -9.014285714285716 24 3 -8.169588744588744 24 4 -8.925829725829727 24 5 -8.352597402597404 24 6 -6.55093795093795 24 7 -7.468470418470417 24 8 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37 3.916702741702742 107 38 4.124062049062049 107 39 4.402994227994228 107 40 4.231132756132756 109 1 -2.6045093795093806 109 2 1.985714285714284 109 3 0.8304112554112564 109 4 -1.9258297258297272 109 5 -2.3525974025974037 109 6 -4.55093795093795 109 7 -1.4684704184704174 109 8 1.9724025974025974 109 9 2.4848484848484826 109 10 -0.17680375180375307 109 11 3.542460317460316 109 12 2.1611832611832593 109 13 1.4105699855699871 109 14 4.2515512265512285 109 15 4.506277056277055 109 16 2.553787878787878 109 17 2.1714646464646474 109 18 -0.7507936507936508 109 19 -0.9386363636363635 109 20 3.1305916305916313 109 21 0.05703463203463244 109 22 3.7504689754689755 109 23 2.9814213564213565 109 24 4.747727272727273 109 25 5.838961038961039 109 26 7.284235209235209 109 27 -0.02759740259740262 109 28 0.33336940836940787 109 29 4.6021284271284255 109 30 4.025252525252526 109 31 9.499134199134197 109 32 7.569588744588744 109 33 5.745959595959596 109 34 9.20494227994228 109 35 5.778427128427129 109 36 12.669552669552669 109 37 12.666702741702743 109 38 6.874062049062049 109 39 7.652994227994228 109 40 7.481132756132756 110 1 -13.10450937950938 110 2 -9.014285714285716 110 3 -8.169588744588744 110 4 -8.925829725829727 110 5 -8.352597402597404 110 6 -6.55093795093795 110 7 -7.468470418470417 110 8 -8.027597402597403 110 9 -6.515151515151517 110 10 -5.676803751803753 110 11 -6.957539682539684 110 12 -6.338816738816741 110 13 -6.089430014430013 110 14 -6.2484487734487715 110 15 -6.493722943722945 110 16 -3.946212121212122 110 17 -3.8285353535353526 110 18 -3.250793650793651 110 19 -2.9386363636363635 110 20 -2.8694083694083687 110 21 -2.9429653679653676 110 22 -3.2495310245310245 110 23 -3.5185786435786435 110 24 -3.252272727272727 110 25 -3.161038961038961 110 26 -3.7157647907647906 110 27 -3.5275974025974026 110 28 -3.166630591630592 110 29 -3.3978715728715745 110 30 -3.474747474747474 110 31 -3.5008658008658022 110 32 -3.430411255411256 110 33 -3.2540404040404036 110 34 -3.795057720057721 110 35 -3.221572871572871 110 36 -2.830447330447331 110 37 -2.833297258297258 110 38 -2.625937950937951 110 39 -2.3470057720057724 110 40 -2.518867243867244 111 1 -13.10450937950938 111 2 -9.014285714285716 111 3 -8.169588744588744 111 4 -8.925829725829727 111 5 -8.352597402597404 111 6 -6.55093795093795 111 7 -7.468470418470417 111 8 -8.027597402597403 111 9 -6.515151515151517 111 10 -5.676803751803753 111 11 -6.957539682539684 111 12 -6.338816738816741 111 13 -6.089430014430013 111 14 -6.2484487734487715 111 15 -6.493722943722945 111 16 -3.946212121212122 111 17 -3.8285353535353526 111 18 -3.250793650793651 111 19 -2.9386363636363635 111 20 -2.8694083694083687 111 21 -2.9429653679653676 111 22 -3.2495310245310245 111 23 -3.5185786435786435 111 24 -3.252272727272727 111 25 -3.161038961038961 111 26 -3.7157647907647906 111 27 -3.5275974025974026 111 28 -3.166630591630592 111 29 -3.3978715728715745 111 30 -3.474747474747474 111 31 -3.5008658008658022 111 32 -3.430411255411256 111 33 -3.2540404040404036 111 34 -3.795057720057721 111 35 -3.221572871572871 111 36 -2.830447330447331 111 37 -2.833297258297258 111 38 -2.625937950937951 111 39 -2.3470057720057724 111 40 -2.518867243867244 117 1 12.89549062049062 117 2 17.985714285714284 117 3 -3.1695887445887436 117 4 20.07417027417027 117 5 17.647402597402596 117 6 17.44906204906205 117 7 4.531529581529583 117 8 13.972402597402597 117 9 14.484848484848483 117 10 7.323196248196247 117 11 13.042460317460316 117 12 -0.3388167388167407 117 13 21.910569985569985 117 14 20.75155122655123 117 15 20.506277056277057 117 16 2.053787878787878 117 17 9.171464646464647 117 18 2.749206349206349 117 19 23.061363636363637 117 20 9.130591630591631 117 21 23.057034632034632 117 22 25.750468975468976 117 23 25.481421356421357 117 24 25.747727272727275 117 25 25.83896103896104 117 26 -3.7157647907647906 117 27 -3.5275974025974026 117 28 -3.166630591630592 117 29 -3.3978715728715745 117 30 -3.474747474747474 117 31 -3.5008658008658022 117 32 -3.430411255411256 117 33 -3.2540404040404036 117 34 -3.795057720057721 117 35 -3.221572871572871 117 36 -2.830447330447331 117 37 -2.833297258297258 117 38 -2.625937950937951 117 39 -2.3470057720057724 117 40 -2.518867243867244 119 1 15.89549062049062 119 2 19.985714285714284 119 3 -0.16958874458874362 119 4 16.07417027417027 119 5 6.647402597402596 119 6 11.44906204906205 119 7 16.531529581529583 119 8 18.9724025974026 119 9 16.484848484848484 119 10 7.323196248196247 119 11 17.042460317460318 119 12 20.66118326118326 119 13 20.910569985569985 119 14 -6.2484487734487715 119 15 -6.493722943722945 119 16 -3.946212121212122 119 17 -3.8285353535353526 119 18 -3.250793650793651 119 19 -2.9386363636363635 119 20 -2.8694083694083687 119 21 -2.9429653679653676 119 22 -3.2495310245310245 119 23 -3.5185786435786435 119 24 -3.252272727272727 119 25 -3.161038961038961 119 26 -3.7157647907647906 119 27 -3.5275974025974026 119 28 -3.166630591630592 119 29 -3.3978715728715745 119 30 -3.474747474747474 119 31 -3.5008658008658022 119 32 -3.430411255411256 119 33 -3.2540404040404036 119 34 -3.795057720057721 119 35 -3.221572871572871 119 36 -2.830447330447331 119 37 -2.833297258297258 119 38 -2.625937950937951 119 39 -2.3470057720057724 119 40 -2.518867243867244 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 825.0 5 45.0 6 74.0 7 31.0 8 17.0 9 20.0 10 32.0 11 33.0 12 26.0 13 27.0 14 20.0 15 22.0 16 13.0 17 14.0 18 19.0 19 22.0 20 36.0 21 43.0 22 34.0 23 22.0 24 23.0 25 13.0 26 19.0 27 22.0 28 16.0 29 13.0 30 3.0 31 3.0 32 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 98.79113498992612 0.2686366689053056 0.33579583613163194 0.6044325050369375 8 95.55555555555556 3.0303030303030303 0.6060606060606061 0.8080808080808081 9 95.15151515151516 0.8080808080808081 3.5690235690235688 0.47138047138047134 10 62.5 16.532258064516128 14.045698924731182 6.922043010752688 11 42.37743451981195 27.199462726662187 16.789791806581597 13.633310946944258 12 34.206989247311824 21.774193548387096 22.849462365591396 21.16935483870968 13 25.85627938213566 19.476158495634653 25.1846877098724 29.482874412357283 14 19.073203492276697 26.326393552719946 32.90799194089993 21.692411014103424 15 32.97515110812626 23.371390194761585 28.27400940228341 15.379449294828746 16 34.721289456010744 24.311618535930155 26.39355271994627 14.573539288112828 17 26.99798522498321 26.326393552719946 33.579583613163194 13.096037609133646 18 25.52048354600403 24.78173270651444 33.445265278710544 16.252518468770987 19 27.75537634408602 29.03225806451613 26.008064516129032 17.20430107526882 20 31.967763599731363 29.348556077904636 25.117528542646074 13.56615177971793 21 22.834116856950974 31.296171927468098 29.684351914036267 16.18535930154466 22 21.02081934184016 26.4607118871726 29.885829415715243 22.632639355271994 23 22.229684351914038 24.177300201477504 27.93821356615178 25.654801880456684 24 21.96104768300873 31.564808596373407 29.348556077904636 17.12558764271323 25 29.05178211163416 29.186281102891726 24.815063887020848 16.94687289845326 26 26.531986531986533 24.646464646464647 24.713804713804713 24.107744107744107 27 18.04713804713805 28.55218855218855 24.175084175084173 29.22558922558923 28 20.900537634408604 33.93817204301075 22.513440860215052 22.647849462365592 29 27.15633423180593 27.762803234501348 23.180592991913745 21.900269541778975 30 22.983870967741936 24.327956989247312 35.483870967741936 17.20430107526882 31 26.12491605104097 27.06514439220954 26.99798522498321 19.811954331766284 32 31.40161725067385 25.60646900269542 23.11320754716981 19.878706199460915 33 26.12491605104097 25.050369375419745 27.669576897246472 21.155137676292814 34 20.550705171255874 27.669576897246472 31.497649429147078 20.28206850235057 35 20.080591000671593 27.40094022834117 37.139019476158495 15.379449294828746 36 20.606060606060606 34.41077441077441 24.579124579124578 20.404040404040405 37 21.42377434519812 30.288784419073206 31.296171927468098 16.991269308260577 38 27.602417730020147 26.930826057756885 28.609805238415042 16.856950973807923 39 23.438549361987913 29.147078576225656 23.774345198119544 23.64002686366689 40 20.68502350570853 28.811282740094025 29.684351914036267 20.819341840161183 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 2.0 18 2.0 19 3.5 20 5.0 21 4.5 22 4.0 23 4.0 24 5.0 25 6.0 26 11.0 27 16.0 28 16.0 29 17.0 30 18.0 31 14.0 32 10.0 33 10.0 34 14.5 35 19.0 36 25.5 37 32.0 38 32.0 39 38.0 40 44.0 41 65.5 42 87.0 43 87.0 44 98.0 45 109.0 46 108.5 47 108.0 48 108.0 49 114.5 50 121.0 51 136.5 52 152.0 53 152.0 54 164.0 55 176.0 56 163.5 57 151.0 58 151.0 59 142.5 60 134.0 61 122.5 62 111.0 63 111.0 64 102.5 65 94.0 66 69.0 67 44.0 68 44.0 69 35.0 70 26.0 71 19.5 72 13.0 73 13.0 74 9.0 75 5.0 76 2.5 77 0.0 78 0.0 79 1.0 80 2.0 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.2686366689053056 9 0.2686366689053056 10 0.0671591672263264 11 0.0 12 0.0671591672263264 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0671591672263264 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.1343183344526528 26 0.2686366689053056 27 0.2686366689053056 28 0.0671591672263264 29 0.33579583613163194 30 0.0671591672263264 31 0.0 32 0.33579583613163194 33 0.0 34 0.0 35 0.0 36 0.2686366689053056 37 0.0 38 0.0 39 0.0 40 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 40 1489.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.7918065815984 #Duplication Level Percentage of deduplicated Percentage of total 1 95.0635751682872 85.35930154466085 2 3.7397157816005984 6.715916722632639 3 0.5235602094240838 1.4103425117528543 4 0.22438294689603588 0.8059100067159166 5 0.0 0.0 6 0.0 0.0 7 0.14958863126402394 0.9402283411685695 8 0.07479431563201197 0.5372733378106112 9 0.07479431563201197 0.6044325050369375 >10 0.14958863126402394 3.626595030221625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCT 38 2.552048354600403 No Hit GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC 16 1.0745466756212223 No Hit GCAGGAGGGGTACCTGGTTGATCCTGCCAGTAGCATATGC 9 0.6044325050369375 No Hit GCAGGAGGGTCACAGATCCTCTTCTGAGATGAGTTTTTGT 8 0.5372733378106112 No Hit GCAGGAGATTGGGCGGATTGAACAAAAACTCATCTCAGAA 7 0.47011417058428473 No Hit GCAGGAGGGTCACAGATCCTCTTCTGAAGGAGATTGGGCG 7 0.47011417058428473 No Hit GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAG 4 0.2686366689053056 No Hit GCAGGAGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAG 4 0.2686366689053056 No Hit GCAGGAGGGGTGTTCTTTATTAATATCCTAACACTCCTCG 4 0.2686366689053056 No Hit GCAGGAGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT 3 0.20147750167897915 No Hit GCAGGAGGGGTGTTTTTTATTAATATCCTAACACTCCTCG 3 0.20147750167897915 No Hit GCAGGAGGGTACATGGTTGATCCTGCCAGTAGCATATGCT 3 0.20147750167897915 No Hit GCAGGAGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.20147750167897915 No Hit GCAGGAGGGTACCTGGTTGCTCCTGCCAGTAGCATATGCT 3 0.20147750167897915 No Hit GCAGGAGGGATGGTATCTATTTATCAGAAAGGCATGTTTC 3 0.20147750167897915 No Hit GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGA 3 0.20147750167897915 No Hit GCAGGAGGGGCTCGTTTTCCCTGAAGGTTCTCTGATCTAG 2 0.1343183344526528 No Hit GCAGGAGGGGGTAATGGTCTGTTTACTTCCTGCGGCTGGA 2 0.1343183344526528 No Hit GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCG 2 0.1343183344526528 No Hit GCAGGAGGGGTTTCCCCCTCATAAGTCATCCCCTCAGAGG 2 0.1343183344526528 No Hit GCAGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.1343183344526528 No Hit GCAGGAGGGAAGCAGAGGTATCAACGCAGTGTCGACTTTT 2 0.1343183344526528 No Hit GCAGGAGGGGGTTGCCACTGTGCAGGTGTTTAGGGCAGGT 2 0.1343183344526528 No Hit GCAGGAGGGTCACGGATCCTCTTCTGAGATGAGTTTTTGT 2 0.1343183344526528 No Hit GCAGGAGGGATCCCTAACTCTCCTAGACTTGATCTGGTTA 2 0.1343183344526528 No Hit GCAGGAGGGCCTAATCCTAGCCCTAGCCCTACACAAATAT 2 0.1343183344526528 No Hit GCAGGAGGGTAACTGGTTGATCCTGCCAGTAGCATATGCT 2 0.1343183344526528 No Hit GCAGGAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGC 2 0.1343183344526528 No Hit GCAGGAGGGGCCGGGGGCATTCGTATTGCGCCGCTAGAGG 2 0.1343183344526528 No Hit GCAGGAGGGATGCCACAACTAGATACATCAACATGATTTA 2 0.1343183344526528 No Hit GCAGGAGGGAGTGCATTGCTGCAGGAGGGTCTCTAAACTT 2 0.1343183344526528 No Hit GCAGGAGGGGGCTGAGACAGGAGGACAGTGGCCTTGCTTC 2 0.1343183344526528 No Hit GCAGGAGGGGTTGTTAGCGAGCAAGTTTCAAGCTACTTGT 2 0.1343183344526528 No Hit GCAGGAGGGCTCCTACTTGGATAAATGTGGTAATTCTAGA 2 0.1343183344526528 No Hit GCAGGAGGGGAGGGATCTGATGCGGGCCGGGGTGTTGAAA 2 0.1343183344526528 No Hit GCAGGAGGGAACTAGTCTGGCAGGCGCTCCGTTCAGTGAA 2 0.1343183344526528 No Hit GCAGGAGGGGAGTCTGAGAATGTGAATGGGGTAAGTGCTT 2 0.1343183344526528 No Hit GCAGGAGGGGTCAAAGATCCTCTTCTGAGATGAGTTTTTG 2 0.1343183344526528 No Hit GCAGGAGGGAGTCTTTCAAGTCGTGTGCATTGCACGTTGG 2 0.1343183344526528 No Hit GCAGGAGATTGGGAGGATTGAACAAAAACTCATCTCAGAA 2 0.1343183344526528 No Hit GCAGGAGGGGGTAGTTTACGGGACTCCGATCGTCTTGCTG 2 0.1343183344526528 No Hit GCAGGAGGGGCAATAATGTCTATTGGCTTTCTAGGCTTTA 2 0.1343183344526528 No Hit GCAGGAGGGGGAGGAGGAAGAGACTGCTGGTTTTGGGGGA 2 0.1343183344526528 No Hit GCAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.1343183344526528 No Hit GCAGGAGGGCAACTTTCGATGGTAGTCGCCGTGCCTACCA 2 0.1343183344526528 No Hit GCAGGAGGGTACCTGGTTGATACTGCCAGTAGCATATGCT 2 0.1343183344526528 No Hit GCAGGAGGGGTTAGCTGACTTGCAACCCTACACGGAGGTA 2 0.1343183344526528 No Hit GCAGGAGGGGGTCTTTGCTAAGGGTCACATTGAAGTAACG 2 0.1343183344526528 No Hit GCAGGAGGGACAGGAAAAGGAGGAGAGCAAGAAGGAGAGA 2 0.1343183344526528 No Hit GCAGGAGGGGTAACTGGTTGATCCTGCCAGTAGCATATGC 2 0.1343183344526528 No Hit GCAGGAGGGACTAATCCTAGCACTAGCCCTACACAAATAT 2 0.1343183344526528 No Hit GCAGGAGGGGTTTCAATGGCTTTCTTCCCTTGACCTTTCC 2 0.1343183344526528 No Hit GCAGGAGGGGGGGAGAATCCGCGTTGAAGAACAGAGCATT 2 0.1343183344526528 No Hit GCAGGAGGGGAGCTACAAGGCTTGCTAGGCATCTGTCTGT 2 0.1343183344526528 No Hit GCAGGAGGGTACCTGGTTGATCCTGCCAGTACCATATGCT 2 0.1343183344526528 No Hit GCAGGAGGGGGATTCGTCTTGACATTGCTCTCTGTGACGA 2 0.1343183344526528 No Hit GCAGGAGGGGCGAATGGCTCATTAAATCAGTTATGGTTCC 2 0.1343183344526528 No Hit GCAGGAGGGATTGTGTTTTAGGACTCATTGGCTGAGTATA 2 0.1343183344526528 No Hit GCAGGAGGGGATTGGGAGAGAGATTGATTCAGATTCGCTC 2 0.1343183344526528 No Hit GCAGGAGGGCGGGGAGGTAGTGACGAAAAATAACAATACA 2 0.1343183344526528 No Hit GCAGGAGGGCCGTGGCTGTAAGTCAGGAGGAGTATGGTGA 2 0.1343183344526528 No Hit GCAGGAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2 0.1343183344526528 No Hit GCAGGAGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAGC 2 0.1343183344526528 No Hit GCAGGAGGGGATTTGTGTGTGTATGTGTGTATATGTGTAT 2 0.1343183344526528 No Hit GCAGGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCC 2 0.1343183344526528 No Hit GCAGGAGGGAGCCGCATCAATATTGTCACTGCCGATTTTG 2 0.1343183344526528 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0671591672263264 0.0 0.0 0.0 0.0 15 0.1343183344526528 0.0 0.0 0.0 0.0 16 0.1343183344526528 0.0 0.0 0.0 0.0 17 0.20147750167897918 0.0 0.0 0.0 0.0 18 0.20147750167897918 0.0 0.0 0.0 0.0 19 0.20147750167897918 0.0 0.0 0.0 0.0 20 0.20147750167897918 0.0 0.0 0.0 0.0 21 0.20147750167897918 0.0 0.0 0.0 0.0 22 0.20147750167897918 0.0 0.0 0.0 0.0 23 0.20147750167897918 0.0 0.0 0.0 0.0 24 0.33579583613163194 0.0 0.0 0.0 0.0 25 0.33579583613163194 0.0 0.0 0.0 0.0 26 0.33579583613163194 0.0 0.0 0.0 0.0 27 0.33579583613163194 0.0 0.0 0.0 0.0 28 0.33579583613163194 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGGGGT 25 9.9473706E-5 34.964287 5 GAGGGGG 35 3.0626688E-7 34.964287 5 GAGGGGA 25 9.9473706E-5 34.964287 5 AGGGGGG 20 0.0016952757 34.964283 6 AGGGGAT 20 0.0016952757 34.964283 6 GGAGGGG 95 0.0 34.964283 4 GGGGATT 20 0.0016952757 34.964283 7 GCAGGAG 150 0.0 32.633335 1 CAGGAGG 135 0.0 32.5083 2 AGGAGGG 135 0.0 32.5083 3 GGAGGGA 30 2.9134535E-4 29.136904 4 >>END_MODULE