FastQCFastQC Report
Fri 17 Jun 2016
SRR1526110_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526110_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29782
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG5341.7930293465851856No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC1690.5674568531327647No Hit
AGATGGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC1370.46000940165200455No Hit
AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC1290.4331475387818145No Hit
AGATGGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT1270.426432073064267No Hit
AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1040.34920421731247064No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC750.25182996440803174No Hit
AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG700.23504130011416294No Hit
AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG660.22161036867906792No Hit
AGATGGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC630.2115371701027466No Hit
AGATGGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC620.2081794372439729No Hit
AGATGGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA540.18131757437378282No Hit
AGATGGGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT490.16452891007991405No Hit
AGATGGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC420.14102478006849775No Hit
AGATGGGGGGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCC390.13095158149217648No Hit
AGATGGGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT360.1208783829158552No Hit
AGATGGGGGAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA350.11752065005708147No Hit
AGATGGGGGGACACTTGGGTCATGTTCAGTTCCTTACAGTCTGGTGCAAAGATCTA340.1141629171983077No Hit
AGATGGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC340.1141629171983077No Hit
AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG330.11080518433953396No Hit
AGATGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA320.1074474514807602No Hit
AGATGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTG310.10408971862198645No Hit
AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC310.10408971862198645No Hit
AGATGGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGACCTAATACATGCCGAC300.10073198576321268No Hit
AGATGGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACCACCCATTCGAACGTC300.10073198576321268No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGATT150.00824002550.1689247
GTGAGAG150.00829531550.08431633
AAGATCT150.00829531550.08431649
ACGATTA150.00829531550.08431648
GCCAAGG150.00829531550.08431646
CTTCCGA150.00829531550.08431650
CGATTAA150.00829531550.08431649
CAATGGT204.1178922E-450.027
ATGGTTA204.1178922E-450.029
AGAGGGT150.00835088149.99999628
GAGTCGA150.00835088149.99999629
CCAATGG204.1523244E-449.9159726
GCCCAAT204.1523244E-449.9159724
GCTAGAG204.1523244E-449.9159712
ATGGCCC204.1523244E-449.9159721
TAGAGAG204.1523244E-449.9159714
CCGGGGA204.1523244E-449.9159723
CCGACCC204.1523244E-449.9159718
TGGGGGT2450.049.9159664
ATGGGGG28250.049.9159623
Produced by FastQC (version 0.11.5)