##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526110_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 29782 Sequences flagged as poor quality 0 Sequence length 56 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.997246659055804 39.0 37.0 39.0 33.0 40.0 2 36.317205023168356 39.0 38.0 39.0 30.0 40.0 3 36.52837284265664 39.0 37.0 39.0 32.0 40.0 4 36.11100664831106 39.0 37.0 39.0 30.0 40.0 5 35.70747431334363 39.0 35.0 39.0 28.0 40.0 6 35.94829091397489 39.0 36.0 39.0 30.0 40.0 7 36.35239406352831 39.0 37.0 39.0 32.0 40.0 8 36.239372775501984 39.0 37.0 39.0 31.0 40.0 9 36.313712980995234 39.0 37.0 39.0 32.0 40.0 10 35.27251359881808 39.0 35.0 39.0 28.0 40.0 11 34.348431938754956 38.0 33.0 39.0 26.0 40.0 12 33.56366261500235 38.0 32.0 39.0 21.0 40.0 13 33.26982741253106 38.0 32.0 39.0 19.0 40.0 14 33.884661876301124 38.0 33.0 39.0 22.0 40.0 15 32.86182929286146 38.0 32.0 39.0 16.0 40.0 16 32.24346920958968 37.0 30.0 39.0 17.0 40.0 17 31.274158887918876 36.0 28.0 38.0 13.0 40.0 18 30.55191054999664 35.0 26.0 38.0 12.0 40.0 19 31.75538916123833 36.0 29.0 38.0 16.0 40.0 20 32.38926197031764 36.0 30.0 39.0 19.0 40.0 21 32.31032167080787 37.0 30.0 39.0 17.0 40.0 22 31.44835806863206 36.0 28.0 39.0 13.0 40.0 23 32.129608488348666 36.0 30.0 39.0 16.0 40.0 24 31.662413538378885 36.0 29.0 39.0 14.0 40.0 25 32.02340339802565 36.0 29.0 39.0 17.0 40.0 26 32.60412329595057 38.0 31.0 39.0 16.0 40.0 27 32.764992277214425 38.0 32.0 39.0 18.0 40.0 28 32.67554227385669 37.0 31.0 39.0 18.0 40.0 29 32.271405546974684 37.0 31.0 39.0 15.0 40.0 30 31.870794439594388 36.0 30.0 39.0 13.0 40.0 31 32.656201732590155 37.0 32.0 39.0 16.0 40.0 32 32.50832717748976 37.0 32.0 39.0 15.0 40.0 33 32.33882882277886 37.0 31.0 39.0 13.0 40.0 34 32.185615472433014 36.0 31.0 39.0 12.0 40.0 35 31.99422469948291 36.0 31.0 39.0 10.0 40.0 36 31.402827211067088 36.0 30.0 38.0 4.0 40.0 37 31.113659257269493 36.0 30.0 38.0 4.0 40.0 38 30.434222013296623 35.0 29.0 38.0 4.0 40.0 39 29.51913907729501 35.0 27.0 38.0 4.0 39.0 40 27.27318514538983 33.0 18.0 38.0 4.0 39.0 41 25.78480290108119 31.0 14.0 37.0 4.0 39.0 42 26.407158686454906 31.0 18.0 37.0 4.0 39.0 43 26.364448324491303 32.0 17.0 37.0 4.0 39.0 44 25.40440534551071 30.0 12.0 36.0 4.0 39.0 45 26.334933852662683 32.0 18.0 37.0 4.0 39.0 46 24.25260224296555 29.0 10.0 36.0 4.0 39.0 47 23.256329326438788 28.0 6.0 35.0 4.0 39.0 48 24.09589685044658 29.0 7.0 35.0 4.0 39.0 49 22.950003357732857 27.0 4.0 35.0 4.0 38.0 50 23.44909676986099 28.0 4.0 35.0 4.0 38.0 51 23.77261433080384 30.0 4.0 36.0 4.0 39.0 52 23.503827815459 29.0 4.0 36.0 4.0 39.0 53 23.239272043516216 29.0 4.0 36.0 4.0 38.0 54 22.93576657041166 29.0 4.0 35.0 4.0 38.0 55 22.43526291048284 28.0 4.0 35.0 4.0 38.0 56 21.97877912833255 27.0 4.0 35.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 2.8092358112163183 1 2 4.169690725920518 1 3 3.283825128955307 1 4 4.353912413839254 1 5 4.450561716536349 1 6 4.647098915352004 1 7 4.0912155389172185 1 8 4.199315595357426 1 9 4.171212433958367 1 10 4.596243113280188 1 11 -0.781421976550071 1 12 -5.690307348130766 1 13 -17.37179560174578 1 14 -6.466551439207333 1 15 2.6411119083156116 1 16 0.44726558137238825 1 17 -5.089085295582947 1 18 -2.3792485581126854 1 19 1.6489535186620188 1 20 0.7287222280062231 1 21 6.73641619426078 1 22 8.177978382473029 1 23 6.703611441155843 1 24 7.304465515733881 1 25 6.8135205440795765 1 26 7.4503204478179015 1 27 7.232286917289958 1 28 6.834978944625057 1 29 7.758300863602617 1 30 7.124642688746086 1 31 6.746713579405618 1 32 6.518250116770325 1 33 6.226508648371873 1 34 6.012890118240964 1 35 6.4153317520208475 1 36 6.135770409200713 1 37 6.340441023474089 1 38 6.712469424727701 1 39 7.6749946013080645 1 40 -7.052661198262271 1 41 4.910352410980803 1 42 4.231063564453031 1 43 -3.2207529034817277 1 44 -11.931411547358845 1 45 2.632255541228247 1 46 -0.08940212273440906 1 47 -5.981479168495268 1 48 0.47732156159563743 1 49 -10.098059860861825 1 50 1.5719694892502716 1 51 8.411619967345025 1 52 7.978058039282427 1 53 8.286536344691768 1 54 8.26739277922357 1 55 8.44187848540388 1 56 10.698225315197064 2 1 2.642185650148676 2 2 4.02841138859884 2 3 3.1931670525632185 2 4 4.345628934778048 2 5 4.773617399923374 2 6 4.005129288108563 2 7 3.368251894186429 2 8 3.662730229503765 2 9 3.68800948872137 2 10 4.742584576694824 2 11 3.8863184744393493 2 12 0.07545427174958164 2 13 2.15190435223489 2 14 3.8891779671433326 2 15 5.113270214804334 2 16 5.832447357718454 2 17 7.550583365254607 2 18 6.34141412021221 2 19 5.267913481846559 2 20 5.361027796344928 2 21 6.150590147321065 2 22 7.694775437236032 2 23 7.5305787674328855 2 24 7.465533164146208 2 25 7.05558220997926 2 26 6.819395459322735 2 27 6.852167267036851 2 28 6.276764494556026 2 29 6.985176151223417 2 30 6.523630263527494 2 31 6.048600371858452 2 32 6.009736541068534 2 33 5.020342058404086 2 34 4.687533468448056 2 35 5.091355682071473 2 36 6.152337367323124 2 37 5.640947236083381 2 38 5.486514523669257 2 39 7.426490229471895 2 40 5.026492046100362 2 41 10.212699396714815 2 42 8.71196554328414 2 43 8.100922200061763 2 44 -0.1007626748323851 2 45 7.809429954481814 2 46 8.156801282695874 2 47 4.244015539282 2 48 8.241242408351276 2 49 0.33452182344558423 2 50 7.861431063111294 2 51 10.785757104942817 2 52 10.820671937119517 2 53 11.402505051548648 2 54 10.115528996434797 2 55 9.033226851717732 2 56 11.346177455095823 3 1 2.566713952035464 3 2 3.6321849735045006 3 3 2.570525543129257 3 4 3.9494025196837086 3 5 4.245315513130919 3 6 4.269280231504787 3 7 3.8399500073939805 3 8 3.7570698521452712 3 9 3.7257453377779726 3 10 3.7425845766948242 3 11 4.9051863989676505 3 12 5.377341064202412 3 13 5.642470389970736 3 14 5.474083627520692 3 15 1.8868551204647126 3 16 7.021126603001473 3 17 7.248696572801773 3 18 8.92631978058957 3 19 6.83395121769561 3 20 6.304424022760017 3 21 7.716627883170123 3 22 7.86458675799075 3 23 6.79472971082911 3 24 7.616476560372625 3 25 6.998978436394349 3 26 6.800527534794426 3 27 6.682355946282133 3 28 6.559783362480559 3 29 6.9474403021668145 3 30 6.844384980508629 3 31 6.275015466198074 3 32 6.085208239181739 3 33 7.095813756517298 3 34 4.404514600523523 3 35 5.581921719807319 3 36 5.944790197511804 3 37 7.584343462498477 3 38 6.920476787820199 3 39 8.124603437019069 3 40 9.271775064968285 3 41 11.363642792941224 3 42 10.787437241397345 3 43 9.81790333213723 3 44 10.729426004412897 3 45 10.469807312972378 3 46 11.251140905337383 3 47 12.885524973244262 3 48 10.731808446087122 3 49 12.976031257407847 3 50 12.012374459337707 3 51 11.31405899173527 3 52 10.650860616364803 3 53 10.421372976076949 3 54 11.757038430397063 3 55 10.033226851717732 3 56 8.666932172076955 4 1 1.9350672968039078 4 2 3.0078281982043293 4 3 2.5803883218599637 4 4 3.4764179570764995 4 5 3.9910273484654013 4 6 2.6753694253298335 4 7 2.3905503504471497 4 8 2.594548411321945 4 9 2.9234297288585864 4 10 2.5834936676039177 4 11 2.9480680456228825 4 12 5.063018594219564 4 13 5.933636770759758 4 14 4.839435257023268 4 15 4.635568671065055 4 16 7.080303275385692 4 17 7.478542198873814 4 18 7.940041907519241 4 19 6.7160266893937255 4 20 5.839586973017312 4 21 6.702048123307339 4 22 5.975650222827795 4 23 4.196959556455184 4 24 6.182514296221683 4 25 6.306868659378917 4 26 4.527371445600608 4 27 5.470091795338735 4 28 4.5361984568201805 4 29 6.2982121718066075 4 30 4.636837810697308 4 31 3.3830772157692515 4 32 3.484436369541946 4 33 5.050788027529304 4 34 3.6819588543828736 4 35 4.658679867320188 4 36 3.3907593227262076 4 37 5.629798007953024 4 38 6.229653460204418 4 39 6.872888171152859 4 40 2.675720176460569 4 41 8.882081901002977 4 42 8.483835183078309 4 43 8.086342440198983 4 44 8.903954306299692 4 45 7.589018290673923 4 46 9.422238675491755 4 47 10.484152760551297 4 48 9.071002271130006 4 49 8.964024396344382 4 50 8.721636895015237 4 51 9.882240809917086 4 52 7.964754269881098 4 53 9.32145873937026 4 54 9.255751980997406 4 55 8.591545891168845 4 56 8.59789272096203 5 1 3.027313286108715 5 2 3.4623736527497826 5 3 2.398124507243203 5 4 4.013405479358141 5 5 4.9068027495349185 5 6 3.6891840420856212 5 7 3.2657731668464365 5 8 4.2117498373469004 5 9 4.066477857200837 5 10 3.664153204145805 5 11 4.584431681986516 5 12 5.025139806340775 5 13 4.362781155786497 5 14 5.217331870213997 5 15 5.540203252170226 5 16 7.849465485724377 5 17 8.37337285841036 5 18 9.16642336919482 5 19 7.0248502188054935 5 20 6.25669927248255 5 21 7.506860957531938 5 22 7.877165374342951 5 23 6.860952426330407 5 24 7.300161355045212 5 25 6.813998784156102 5 26 5.874519395689731 5 27 5.582836893377948 5 28 5.041100417604497 5 29 5.85347063882978 5 30 6.068210359716915 5 31 5.021223383327186 5 32 5.904668098597206 5 33 5.002214052484742 5 34 5.512172758126187 5 35 3.670711952881682 5 36 5.385411729143325 5 37 4.525519933086709 5 38 5.670384297994083 5 39 6.361747351188505 5 40 7.806736219241319 5 41 10.632973166600127 5 42 10.11485122585906 5 43 9.048018019521624 5 44 9.261797443554595 5 45 9.586344493882478 5 46 10.904858996347372 5 47 11.867396967324918 5 48 11.047383732805585 5 49 13.65564649973119 5 50 11.178856146352135 5 51 10.510137423107818 5 52 9.920190990023698 5 53 9.269319701937103 5 54 10.018229699357477 5 55 8.38209847583908 5 56 8.442366874259715 6 1 2.449090507635624 6 2 4.143224716579567 6 3 3.4135604688618955 6 4 4.476901516070697 6 5 5.0369654529141386 6 6 4.721307529778571 6 7 4.055927526462625 6 8 4.36485788907823 6 9 4.068980986111974 6 10 4.934073938396949 6 11 5.2652827458163 6 12 6.443579522652833 6 13 6.029865010605025 6 14 5.616998119692511 6 15 7.247754357718829 6 16 7.206995731865387 6 17 8.803493842974394 6 18 8.835593164772629 6 19 7.285175625563941 6 20 6.702256218665276 6 21 7.757173848645831 6 22 7.671491615477699 6 23 7.159241954907792 6 24 7.56549301962594 6 25 7.247874462086845 6 26 5.105224443666366 6 27 6.741368391083416 6 28 6.701092073841458 6 29 6.57770927045264 6 30 6.21130589580369 6 31 6.576500813448174 6 32 5.497492462385274 6 33 6.493244507219824 6 34 5.64859328378326 6 35 6.679472904070671 6 36 7.868515608993132 6 37 8.052428568881457 6 38 8.042516129450064 6 39 8.598710221442992 6 40 6.864308184197512 6 41 12.729760817830826 6 42 10.534125318474825 6 43 12.035085186814069 6 44 11.696507497789053 6 45 11.678960263594618 6 46 13.634521073944367 6 47 13.996725294400424 6 48 13.22719182625573 6 49 14.401161727098739 6 50 14.59687867451234 6 51 11.228952608756547 6 52 11.868042471041637 6 53 12.288093168770647 6 54 12.139214263395857 6 55 12.679553628112753 6 56 10.595474925991045 7 1 2.6702681424698653 7 2 3.5553969085637362 7 3 1.8737287024096432 7 4 3.398722396822805 7 5 4.400646798782525 7 6 4.15475631750742 7 7 3.458203627402753 7 8 3.5929627876433017 7 9 3.2812521390065825 7 10 4.277468297625056 7 11 4.816989821521396 7 12 5.660798721069462 7 13 4.797801675622342 7 14 5.55613715977168 7 15 6.110198692206705 7 16 7.096159512172161 7 17 8.313637336294537 7 18 9.024608503713747 7 19 7.105561573114652 7 20 5.835358643207584 7 21 7.123824021827428 7 22 7.86941343635845 7 23 7.6293062794995805 7 24 8.624374761337961 7 25 7.041540963818658 7 26 6.262572291266565 7 27 6.161952260455013 7 28 6.437361247517856 7 29 7.204131833540227 7 30 6.264744787441494 7 31 5.490899625917248 7 32 5.549446940366472 7 33 4.936094581440511 7 34 5.6243478607253685 7 35 4.806142869434353 7 36 7.301964396721981 7 37 7.979692299707782 7 38 7.065277138851357 7 39 8.112317346628544 7 40 8.002358654261837 7 41 12.425950822778876 7 42 12.705822498088882 7 43 12.159281129416744 7 44 11.514419422578765 7 45 11.974853385811343 7 46 12.499934235745453 7 47 14.12685889162952 7 48 12.184638634766369 7 49 13.959796066534658 7 50 14.301975161400016 7 51 11.895454340572481 7 52 12.17828492527222 7 53 10.961522164317405 7 54 11.003637816092542 7 55 11.985837645925717 7 56 11.637533312928205 8 1 2.7500023617389715 8 2 3.9623736527497826 8 3 2.5381805296521662 8 4 4.531612762271308 8 5 4.758343365781414 8 6 4.561733061693467 8 7 3.862411822308623 8 8 4.305587372360911 8 9 3.918018473447333 8 10 4.647346481456729 8 11 5.513003110557946 8 12 6.62037790157887 8 13 6.845974433097421 8 14 6.3307772483652585 8 15 7.369334904831295 8 16 8.065151760234176 8 17 8.069451289782908 8 18 8.756059223536557 8 19 8.608883832250871 8 20 7.354738488168827 8 21 8.375208296467516 8 22 8.662879660057236 8 23 7.963193322688944 8 24 7.277752391459778 8 25 8.001673854184109 8 26 5.913735081964241 8 27 4.839139414386356 8 28 4.3338175044392315 8 29 6.129381002975439 8 30 5.8987425726020675 8 31 4.532427865119903 8 32 5.23660087170645 8 33 5.006415733157009 8 34 4.706850629274651 8 35 4.627294585934905 8 36 4.851798283765174 8 37 5.655772033927047 8 38 5.631168611719573 8 39 6.196481244745932 8 40 3.392170392910785 8 41 10.54333731225839 8 42 9.081237780480908 8 43 8.926169280025821 8 44 10.177763830109217 8 45 8.998109199764833 8 46 11.033710536963618 8 47 11.562074838473375 8 48 10.827495777623511 8 49 11.883937816257802 8 50 10.624234297612642 8 51 9.43310661078289 8 52 11.37817418330101 8 53 10.06063622854775 8 54 9.19189916714459 8 55 9.660809960432918 8 56 7.679061552130861 9 1 3.158640235493124 9 2 4.229815513214902 9 3 2.7341938186887162 9 4 4.282443327055361 9 5 4.633204938317405 9 6 4.92219817797254 9 7 4.272157115774846 9 8 4.104590694620043 9 9 3.8626474878437875 9 10 4.742584576694824 9 11 5.189082844777211 9 12 6.242194069906667 9 13 5.728034233761878 9 14 4.393346462097263 9 15 6.6218265991834535 9 16 7.9101130005442535 9 17 8.802009429317788 9 18 9.931585247899793 9 19 8.105561573114652 9 20 6.765591201347121 9 21 7.868010068339057 9 22 7.8461576224049665 9 23 7.303724884150739 9 24 8.554607319477498 9 25 7.3671223591674995 9 26 6.332339733127029 9 27 6.557301097664318 9 28 6.5536403172853 9 29 6.506457414935571 9 30 5.985675019999633 9 31 5.3281089282428304 9 32 5.828516707808326 9 33 6.65702481399865 9 34 5.833650186306766 9 35 6.1317242647831876 9 36 6.325220210675472 9 37 6.584343462498477 9 38 8.716439929549026 9 39 8.647201067558775 9 40 4.676777258912999 9 41 9.914322915802128 9 42 11.263962032972596 9 43 11.624397408486512 9 44 11.816745003974109 9 45 11.532992920695065 9 46 12.174352840396619 9 47 14.336161217210918 9 48 13.998592123138462 9 49 15.285377461883492 9 50 13.278719347446525 9 51 14.221035735921316 9 52 12.875959343876868 9 53 14.426638443387173 9 54 13.46875409516231 9 55 14.776535320344319 9 56 13.288696103625881 10 1 2.6547642665008766 10 2 3.7957069860831183 10 3 3.419132910604546 10 4 4.103041333699878 10 5 5.09167669911475 10 6 4.585542585502992 10 7 4.171935631832433 10 8 3.710349277122816 10 9 4.227542282971143 10 10 4.88544171955197 10 11 5.893955491510326 10 12 6.73942552062649 10 13 6.393593480716472 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36 6.4024895280048035 115 37 7.068214430240413 115 38 6.878480439676558 115 39 7.577433625698312 115 40 10.339943050360862 115 41 7.979589232381276 115 42 9.744832250526986 115 43 7.046752997383734 115 44 0.04105108049324002 115 45 7.223915651377737 115 46 3.7587489394213733 115 47 -6.378017327425244 115 48 3.5072192799276607 115 49 -3.047705808934211 115 50 3.509026924340752 115 51 3.636639636896561 115 52 4.911968346127431 115 53 4.960021789223632 115 54 3.709564297712948 115 55 4.862806888236296 115 56 7.377218234158512 116 1 2.742483564746486 116 2 3.9886894422234676 116 3 3.2035940634867544 116 4 3.6331165216698054 116 5 4.8247594058816645 116 6 4.6143646406408365 116 7 3.981459441356243 116 8 3.8732565452932377 116 9 3.194960829337056 116 10 3.453110892484297 116 11 3.6370632609338855 116 12 3.568999455463583 116 13 5.116651124826749 116 14 3.9159902809467084 116 15 -2.9051011853942725 116 16 4.266906146199094 116 17 4.77997760557238 116 18 4.277362481681923 116 19 5.729184584130568 116 20 5.416141996940752 116 21 5.7235792237858085 116 22 5.754358356799088 116 23 6.395524149756618 116 24 6.840409033063786 116 25 6.321222726364564 116 26 5.308471924069501 116 27 3.7727233742861053 116 28 4.128303719978078 116 29 4.738403559366414 116 30 5.584206231749938 116 31 4.351976737300358 116 32 4.162666034613714 116 33 5.272079893558011 116 34 4.21186316060297 116 35 4.4756655132531975 116 36 4.156309561960658 116 37 4.873817146709005 116 38 3.6925721204915014 116 39 5.972170467803572 116 40 7.314476157321813 116 41 5.4167708962182886 116 42 5.254170111307971 116 43 0.42366301436166154 116 44 -1.6881509568582018 116 45 3.9717934102911485 116 46 -0.10288094173312601 116 47 -6.757474033707076 116 48 -0.2364139968125798 116 49 -2.9331047902584864 116 50 -0.12764540163547977 116 51 2.3930063601563205 116 52 2.361883456483966 116 53 2.035573571906145 116 54 2.044029492959126 116 55 0.7422635945181284 116 56 1.3670314769428913 117 1 1.9880975998342052 117 2 3.166077356453485 117 3 1.9297149211865587 117 4 3.1876974183559668 117 5 3.9699835774216297 117 6 3.5432145431749476 117 7 3.1110890709858694 117 8 3.4405080072815437 117 9 3.1296586850875485 117 10 3.8907327248429695 117 11 -2.3044572069023737 117 12 -12.456341675140703 117 13 -11.299528212405221 117 14 -2.9417095241215137 117 15 -7.736485200988813 117 16 -8.971885276802858 117 17 0.7916735120051293 117 18 -2.9185439510666136 117 19 1.1512118745789124 117 20 0.09547922890956428 117 21 4.528647449906668 117 22 4.877165374342951 117 23 4.087531947027571 117 24 4.849180962888347 117 25 4.382626235136488 117 26 4.135957304186462 117 27 4.312684387931331 117 28 3.7121243827461043 117 29 4.9732963468907805 117 30 3.081282255141751 117 31 2.487454320146359 117 32 3.1704633055688802 117 33 2.982606209347484 117 34 2.455527877951898 117 35 1.7622152208555377 117 36 4.0607929927598825 117 37 4.769528647683661 117 38 3.8163537969047567 117 39 5.170026218290904 117 40 7.371006371746763 117 41 4.199421968343042 117 42 3.6791213783645027 117 43 -5.272243418386875 117 44 -4.457156804811419 117 45 1.775886977542612 117 46 -2.733485230232148 117 47 -2.4114701350715997 117 48 -1.3709169207891883 117 49 -3.5578611255411374 117 50 -1.3069826336042887 117 51 3.2399849176611966 117 52 1.6347879399147658 117 53 2.3542870221985446 117 54 3.9855499607953853 117 55 2.0444078440308004 117 56 6.078532451601763 118 1 2.071430933167541 118 2 3.062373652749784 118 3 2.716751958223597 118 4 3.517327047985596 118 5 3.3773909848290344 118 6 3.7617330616934694 118 7 3.3314594413562446 118 8 3.5127302295037666 118 9 3.237066092494956 118 10 3.892584576694823 118 11 -1.065568318013483 118 12 -9.19152686032589 118 13 -9.475454138331148 118 14 -10.728746561158552 118 15 -10.073522238025852 118 16 -13.651514906432489 118 17 -0.13054871021709147 118 18 -6.946321728844392 118 19 -1.0339733106062745 118 20 -1.128594845164507 118 21 5.015684486943709 118 22 6.0938320410096125 118 23 6.056050465546093 118 24 5.332514296221682 118 25 5.1159595684698225 118 26 6.163735081964241 118 27 6.720091795338735 118 28 6.2861984568201805 118 29 6.043666717261157 118 30 5.636837810697308 118 31 4.896713579405617 118 32 5.638981824087402 118 33 5.332606209347485 118 34 4.359231581655603 118 35 5.917770776411096 118 36 5.458941140908028 118 37 4.78434346249848 118 38 5.797835278386238 118 39 6.427433625698313 118 40 9.130265631006022 118 41 8.54571826463934 118 42 7.3883806376237615 118 43 2.249978803835347 118 44 -1.4960456937003066 118 45 5.348109199764835 118 46 -1.7186704154173356 118 47 -2.5522108758123387 118 48 -0.16536136523363254 118 49 -1.604157421837435 118 50 0.4170914404697825 118 51 4.2640589917352685 118 52 3.0829360880629153 118 53 2.2487314666429867 118 54 1.7466610719064946 118 55 1.4870004366233935 118 56 3.293347266416575 119 1 2.121430933167538 119 2 2.862373652749781 119 3 2.246751958223598 119 4 3.7373270479855947 119 5 3.8573909848290313 119 6 3.2617330616934694 119 7 3.441459441356244 119 8 3.382730229503764 119 9 2.257066092494952 119 10 3.0225845766948254 119 11 -0.8955683180134848 119 12 -10.92152686032589 119 13 -0.9054541383311481 119 14 -3.6987465611585506 119 15 -11.773522238025851 119 16 -10.641514906432487 119 17 -0.1105487102170919 119 18 -5.336321728844393 119 19 -0.5939733106062732 119 20 0.021405154835491658 119 21 4.245684486943706 119 22 5.303832041009613 119 23 4.686050465546089 119 24 4.182514296221683 119 25 4.995959568469825 119 26 4.95373508196424 119 27 6.200091795338732 119 28 4.586198456820178 119 29 6.223666717261157 119 30 5.23683781069731 119 31 5.186713579405616 119 32 4.808981824087397 119 33 4.902606209347486 119 34 4.799231581655601 119 35 5.357770776411094 119 36 6.488941140908029 119 37 5.14434346249848 119 38 7.4978352783862405 119 39 7.497433625698314 119 40 6.990265631006022 119 41 6.055718264639339 119 42 3.858380637623764 119 43 -5.450021196164652 119 44 -2.186045693700308 119 45 2.8381091997648333 119 46 -0.6386704154173337 119 47 -1.3922108758123386 119 48 -4.615361365233632 119 49 -1.6141574218374366 119 50 1.6070914404697838 119 51 4.554058991735268 119 52 5.862936088062913 119 53 5.478731466642987 119 54 6.016661071906498 119 55 6.557000436623394 119 56 8.213347266416577 120 1 2.2445078562444607 120 2 3.0008351912113227 120 3 2.58213657360821 120 4 3.6250193556778996 120 5 4.338929446367494 120 6 3.67711767707808 120 7 3.2122286721254767 120 8 3.4704225371960717 120 9 3.0370660924949533 120 10 3.3964307305409775 120 11 -1.261722164167331 120 12 -10.833834552633583 120 13 -5.456223369100378 120 14 -7.901823484235475 120 15 1.2649393004356888 120 16 -11.255361060278641 120 17 0.03098975132136772 120 18 -4.734783267305932 120 19 0.08141130477834224 120 20 -0.9401333067029682 120 21 5.911838333097553 120 22 7.351524348701922 120 23 6.10605046554609 120 24 7.259437373144763 120 25 6.5236518761621305 120 26 7.3368120050411605 120 27 6.7585533338002755 120 28 6.824659995281721 120 29 7.535974409568848 120 30 7.829145503005002 120 31 6.8620981947902315 120 32 6.7659049010104795 120 33 6.674913901655177 120 34 6.966923889347907 120 35 7.087001545641861 120 36 7.978171910138794 120 37 8.27665115480617 120 38 8.605527586078544 120 39 8.731279779544465 120 40 9.245650246390635 120 41 -2.73505096612989 120 42 -0.061619362376237774 120 43 -6.8192519653954236 120 44 -13.076814924469538 120 45 -1.1557369540813198 120 46 -6.572516569263488 120 47 -3.498364721966187 120 48 -2.315361365233631 120 49 -12.584926652606665 120 50 -0.19444702106867595 120 51 3.814058991735269 120 52 4.052166857293685 120 53 5.0679622358737575 120 54 5.288968764214189 120 55 5.598538898161852 120 56 8.970270343339653 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 861.0 5 169.0 6 41.0 7 104.0 8 203.0 9 164.0 10 138.0 11 149.0 12 121.0 13 85.0 14 98.0 15 110.0 16 126.0 17 168.0 18 242.0 19 303.0 20 346.0 21 437.0 22 542.0 23 643.0 24 768.0 25 866.0 26 899.0 27 928.0 28 1036.0 29 1125.0 30 1270.0 31 1415.0 32 1545.0 33 1784.0 34 1978.0 35 2094.0 36 2325.0 37 2435.0 38 2237.0 39 2027.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 98.97253374521523 0.8159290846820226 0.1208783829158552 0.09065878718689141 8 98.81807803371164 0.8327177489758916 0.21825263582029414 0.13095158149217648 9 95.48033981397536 2.279977166649877 1.4136530002350494 0.82603001913972 10 63.998388288227794 19.609159895238733 8.632731179907328 7.75972063662615 11 46.679202202672755 21.788328520582905 15.683970183332214 15.848499093412126 12 35.97474984890202 12.608286884695454 20.357934322745283 31.05902894365724 13 25.69001410247801 14.58934927137197 23.168356725538917 36.552279900611104 14 24.078302330266606 18.561547243301323 32.08313746558324 25.277012960848833 15 31.660648601221613 17.925960921945126 24.50646238990315 25.906928086930115 16 38.44189874693453 16.98525212483623 23.112843081264487 21.46000604696476 17 30.22670025188917 19.68429890848027 28.607892527287994 21.48110831234257 18 28.628030353905043 20.33778792559264 27.325230004700824 23.70895171580149 19 34.69278059596197 24.67497564416972 18.37605401955185 22.256189740316458 20 32.93219940166056 26.616020706578375 19.056102726142054 21.395677165619013 21 28.93022631119468 24.356994157544825 25.166207776509303 21.54657175475119 22 25.25350883083742 20.22026727553556 26.129877106977368 28.396346786649655 23 27.462897051910552 20.102746625478478 22.674769995299172 29.759586327311798 24 29.640698455339155 19.78173270651444 25.117528542646074 25.460040295500335 25 30.87850090670965 20.797232856471222 20.179327019947614 28.144939216871517 26 24.76401625852397 19.752091101481405 24.747220262689375 30.73667237730525 27 24.04233870967742 18.615591397849464 22.0127688172043 35.329301075268816 28 23.823114632999797 27.02639178026996 20.985830367335975 28.164663219394264 29 34.796354219217704 21.68970504153634 18.682944876063633 24.830995863182324 30 30.474078553909216 19.544400766051808 27.10076269193294 22.880757988106037 31 31.86473689512744 22.41176668121831 23.019577554652606 22.703918869001647 32 39.18651126859907 18.463036979813925 21.015685352500586 21.334766399086423 33 31.382370738922884 20.207759026423723 21.9760640086062 26.433806226047203 34 25.70803901782711 25.455768583921962 27.151025899764548 21.685166498486378 35 26.313842843213074 25.61447160485525 29.161763222487476 18.9099223294442 36 26.629266326256385 31.62120397742542 19.756113947863476 21.993415748454716 37 29.66474999159353 24.523353172601634 24.62759339587747 21.18430343992737 38 32.49436387496215 28.288300413876645 18.227396614960124 20.989939096201084 39 30.779574410864964 22.768682556224157 20.028910478367568 26.422832554543312 40 24.081784638299315 18.445550252191868 21.217968247520393 36.25469686198842 41 19.633712056978126 17.296930642699678 19.840596913684923 43.22876038663727 42 26.755109599649817 22.54284656049025 21.923297080709787 28.778746759150142 43 20.559709032127703 16.340001347073482 27.800229002492088 35.30006061830673 44 24.617200674536257 19.11973018549747 23.069139966273188 33.193929173693085 45 27.01947986407832 21.703731117316558 25.431484035931767 25.84530498267335 46 22.083347370548797 25.071589798874776 24.39780345652394 28.447259374052486 47 19.190146785895056 22.32495360215961 25.574489623755692 32.91040998818964 48 24.575730080317236 21.97466142420271 28.030379406526194 25.41922908895386 49 23.56012125294712 22.556416301785113 24.4493095318289 29.434152913438865 50 27.034209021494167 20.18567728480608 26.189915570654914 26.59019812304484 51 23.257223681551828 22.78238027884421 29.64235199030107 24.31804404930289 52 22.983246989167732 18.862275449101794 30.777770302092446 27.376707259638028 53 22.782977577267523 18.746212376270957 32.082014679146184 26.388795367315332 54 24.86113448914324 22.27571115973742 28.719070863490998 24.144083487628347 55 22.798276326420684 22.922838674925934 26.548612981416642 27.730272017236736 56 27.07078186530241 20.56140823264111 24.852748140419372 27.515061761637106 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 1.0 10 4.5 11 8.0 12 11.5 13 16.5 14 18.0 15 23.0 16 28.0 17 24.0 18 20.0 19 26.0 20 32.0 21 26.0 22 18.0 23 16.0 24 25.0 25 34.0 26 40.5 27 47.0 28 45.0 29 59.0 30 75.0 31 91.5 32 108.0 33 125.0 34 142.0 35 192.0 36 242.0 37 270.0 38 336.5 39 375.0 40 394.0 41 413.0 42 474.0 43 535.0 44 752.0 45 969.0 46 1352.5 47 1619.5 48 1503.0 49 1430.0 50 1357.0 51 1431.0 52 1505.0 53 1569.5 54 1774.0 55 1914.0 56 1966.0 57 2018.0 58 2257.5 59 2497.0 60 2542.5 61 2588.0 62 2526.0 63 2129.0 64 1794.0 65 1672.0 66 1550.0 67 1346.5 68 1143.0 69 992.0 70 841.0 71 736.5 72 581.0 73 530.0 74 399.5 75 269.0 76 218.5 77 168.0 78 132.5 79 83.5 80 70.0 81 46.0 82 22.0 83 14.0 84 6.0 85 4.0 86 2.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.003357732858773756 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5003021959572896 16 0.05036599288160634 17 0.023504130011416293 18 0.0 19 0.05036599288160634 20 0.11080518433953396 21 0.0 22 0.0 23 0.0 24 0.006715465717547512 25 0.013430931435095024 26 0.04365052716405883 27 0.07387012289302262 28 0.0 29 0.16452891007991405 30 0.06379692431670136 31 0.010073198576321269 32 0.0302195957289638 33 0.1208783829158552 34 0.17460210865623532 35 0.137667047209724 36 0.047008260022832586 37 0.1443825129272715 38 0.2115371701027466 39 0.11752065005708147 40 0.8092136189644751 41 0.9972466590558055 42 0.2786918272782218 43 0.2954804915720905 44 0.44322073735813583 45 0.1981062386676516 46 0.3324155530186018 47 0.49358673023974214 48 0.0839433214693439 49 0.30891142300718555 50 0.17795984151500907 51 0.2954804915720905 52 0.18803304009133034 53 0.26861862870190045 54 0.2585454301255792 55 0.26190316298435296 56 0.23839903297293666 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 29782.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.67812772815795 #Duplication Level Percentage of deduplicated Percentage of total 1 88.5008085008085 64.32073064267007 2 6.772926772926772 9.844872741924652 3 1.8895818895818897 4.119938217715399 4 0.8131208131208132 2.363843932576724 5 0.4943404943404944 1.7963870794439594 6 0.3465003465003465 1.5109797864481902 7 0.21714021714021714 1.1046941105365657 8 0.18942018942018943 1.101336377677792 9 0.11088011088011088 0.7252702974951313 >10 0.6098406098406098 7.7731515680612455 >50 0.02772002772002772 1.3095158149217647 >100 0.023100023100023098 2.2362500839433213 >500 0.00462000462000462 1.7930293465851856 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 534 1.7930293465851856 No Hit AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 169 0.5674568531327647 No Hit AGATGGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGAC 137 0.46000940165200455 No Hit AGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 129 0.4331475387818145 No Hit AGATGGGGGGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTATCAGAT 127 0.426432073064267 No Hit AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 104 0.34920421731247064 No Hit AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 75 0.25182996440803174 No Hit AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 70 0.23504130011416294 No Hit AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCAAGATTAAG 66 0.22161036867906792 No Hit AGATGGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTC 63 0.2115371701027466 No Hit AGATGGGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 62 0.2081794372439729 No Hit AGATGGGGGGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAA 54 0.18131757437378282 No Hit AGATGGGGGACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTT 49 0.16452891007991405 No Hit AGATGGGGGAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTC 42 0.14102478006849775 No Hit AGATGGGGGGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCC 39 0.13095158149217648 No Hit AGATGGGGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGT 36 0.1208783829158552 No Hit AGATGGGGGAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCA 35 0.11752065005708147 No Hit AGATGGGGGGACACTTGGGTCATGTTCAGTTCCTTACAGTCTGGTGCAAAGATCTA 34 0.1141629171983077 No Hit AGATGGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGCGCTAATACATGCCGAC 34 0.1141629171983077 No Hit AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 33 0.11080518433953396 No Hit AGATGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA 32 0.1074474514807602 No Hit AGATGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTG 31 0.10408971862198645 No Hit AGATGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 31 0.10408971862198645 No Hit AGATGGGGGCTCCTACTTGGATAACTGTGGTAATTCTAGACCTAATACATGCCGAC 30 0.10073198576321268 No Hit AGATGGGGGGGGCCGATCGCACGCCCCCCGTGGCGGCGACCACCCATTCGAACGTC 30 0.10073198576321268 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.003357732858773756 0.0 0.0 0.0 0.0 13 0.003357732858773756 0.0 0.0 0.0 0.0 14 0.003357732858773756 0.0 0.0 0.0 0.0 15 0.006715465717547512 0.0 0.0 0.0 0.0 16 0.010073198576321269 0.0 0.0 0.0 0.0 17 0.013430931435095024 0.0 0.0 0.0 0.0 18 0.01678866429386878 0.0 0.0 0.0 0.0 19 0.023504130011416293 0.0 0.0 0.0 0.0 20 0.03357732858773756 0.0 0.0 0.0 0.0 21 0.03357732858773756 0.0 0.0 0.0 0.0 22 0.03693506144651131 0.0 0.0 0.0 0.0 23 0.03693506144651131 0.0 0.0 0.0 0.0 24 0.03693506144651131 0.0 0.0 0.0 0.0 25 0.03693506144651131 0.0 0.0 0.0 0.0 26 0.03693506144651131 0.0 0.0 0.0 0.0 27 0.040292794305285075 0.0 0.0 0.0 0.0 28 0.040292794305285075 0.0 0.0 0.0 0.0 29 0.047008260022832586 0.0 0.0 0.0 0.0 30 0.047008260022832586 0.0 0.0 0.0 0.0 31 0.05036599288160634 0.0 0.0 0.0 0.0 32 0.0537237257403801 0.0 0.0 0.0 0.0 33 0.0537237257403801 0.0 0.0 0.0 0.0 34 0.05708145859915385 0.0 0.0 0.0 0.0 35 0.06043919145792761 0.0 0.0 0.0 0.0 36 0.06043919145792761 0.0 0.0 0.0 0.0 37 0.06043919145792761 0.0 0.0 0.0 0.0 38 0.06379692431670136 0.0 0.0 0.0 0.0 39 0.07051239003424888 0.0 0.0 0.0 0.0 40 0.07051239003424888 0.0 0.0 0.0 0.0 41 0.07051239003424888 0.0 0.0 0.0 0.0 42 0.07051239003424888 0.0 0.0 0.0 0.0 43 0.07387012289302262 0.0 0.0 0.0 0.0 44 0.08058558861057015 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAGATT 15 0.008240025 50.16892 47 GTGAGAG 15 0.008295315 50.084316 33 AAGATCT 15 0.008295315 50.084316 49 ACGATTA 15 0.008295315 50.084316 48 GCCAAGG 15 0.008295315 50.084316 46 CTTCCGA 15 0.008295315 50.084316 50 CGATTAA 15 0.008295315 50.084316 49 CAATGGT 20 4.1178922E-4 50.0 27 ATGGTTA 20 4.1178922E-4 50.0 29 AGAGGGT 15 0.008350881 49.999996 28 GAGTCGA 15 0.008350881 49.999996 29 CCAATGG 20 4.1523244E-4 49.91597 26 GCCCAAT 20 4.1523244E-4 49.91597 24 GCTAGAG 20 4.1523244E-4 49.91597 12 ATGGCCC 20 4.1523244E-4 49.91597 21 TAGAGAG 20 4.1523244E-4 49.91597 14 CCGGGGA 20 4.1523244E-4 49.91597 23 CCGACCC 20 4.1523244E-4 49.91597 18 TGGGGGT 245 0.0 49.915966 4 ATGGGGG 2825 0.0 49.915962 3 >>END_MODULE