FastQCFastQC Report
Fri 17 Jun 2016
SRR1526109_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526109_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3280
Sequences flagged as poor quality0
Sequence length40
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC160.4878048780487805No Hit
AGATGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT70.2134146341463415No Hit
AGATGGGGGATGTCATTCTGTGCATCTGGGGTTATCTGGG60.1829268292682927No Hit
AGATGGGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC50.1524390243902439No Hit
AGATGGGGGATTAGTGATGATGTGATTGTCGGCCGGTGCC40.12195121951219512No Hit
AGATGGGGGGTGTAGAACTGCTCCAGGCTTTCTTCCTTGT40.12195121951219512No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCCGTGGGTACGAGC40.12195121951219512No Hit
AGATGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT40.12195121951219512No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC40.12195121951219512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGCT251.4140629E-434.74603320
TGGCTCA200.00214801334.74603322
GGGGGAT357.689705E-733.6769265
GGGGGAG357.689705E-733.6769265
ATGGGGG2600.033.676923
ATGGGGA502.1100277E-1033.676923
TGGGGGT405.0660674E-833.676924
TGGGGGG750.033.676924
TGGGGGA1300.033.676924
GGGGCTG200.002516556833.676929
GGGCTGG251.7123159E-433.6769210
AGATGGG3250.033.1588171
GATGGGG3150.033.142372
ATGGCTC304.1292087E-428.95502721
GGGGGGT304.996236E-428.0641025
GGGGGGC304.996236E-428.0641027
TGGGGAG250.007506696526.9415384
GGGGGCT250.007506696526.9415388
GGGGGAC250.007506696526.9415385
GGGGGAA503.6043275E-726.9415385
Produced by FastQC (version 0.11.5)