##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526109_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3280
Sequences flagged as poor quality	0
Sequence length	40
%GC	51
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.984146341463415	33.0	28.0	36.0	19.0	38.0
2	28.085670731707317	33.0	22.0	36.0	11.0	38.0
3	27.194817073170732	31.0	23.0	35.0	10.0	38.0
4	27.54359756097561	33.0	20.0	37.0	4.0	38.0
5	26.56341463414634	31.0	19.0	36.0	4.0	38.0
6	26.057012195121953	30.0	19.0	35.0	4.0	38.0
7	23.908536585365855	26.0	15.0	34.0	4.0	37.0
8	26.224085365853657	31.0	20.0	35.0	4.0	38.0
9	23.41859756097561	26.0	15.0	33.0	4.0	37.0
10	22.946646341463413	27.0	13.0	32.0	4.0	36.0
11	21.22012195121951	23.0	12.0	32.0	4.0	36.0
12	21.26219512195122	23.0	11.0	32.0	4.0	36.0
13	22.179878048780488	25.0	11.0	33.0	4.0	36.0
14	22.347560975609756	25.0	10.0	33.0	4.0	36.0
15	21.378963414634146	23.0	4.0	33.0	4.0	36.0
16	19.835670731707317	21.0	4.0	31.0	4.0	35.0
17	19.528658536585365	22.0	4.0	31.0	4.0	35.0
18	19.603963414634148	22.0	4.0	31.0	4.0	35.0
19	17.78170731707317	18.0	4.0	30.0	4.0	35.0
20	18.9	20.0	4.0	31.0	4.0	35.0
21	17.494512195121953	16.0	4.0	31.0	4.0	35.0
22	16.88719512195122	15.0	4.0	30.0	4.0	34.0
23	17.114634146341462	15.0	4.0	31.0	4.0	35.0
24	16.26829268292683	13.0	4.0	30.0	4.0	34.0
25	15.617073170731707	4.0	4.0	30.0	4.0	34.0
26	13.346646341463414	4.0	4.0	25.0	4.0	33.0
27	13.146341463414634	4.0	4.0	26.0	4.0	33.0
28	12.586585365853658	4.0	4.0	24.0	4.0	32.0
29	12.403963414634147	4.0	4.0	23.0	4.0	33.0
30	11.830792682926829	4.0	4.0	22.0	4.0	33.0
31	11.18780487804878	4.0	4.0	20.0	4.0	32.0
32	10.572865853658536	4.0	4.0	16.0	4.0	31.0
33	9.902743902439024	4.0	4.0	13.0	4.0	31.0
34	9.392073170731708	4.0	4.0	10.0	4.0	29.0
35	9.048475609756098	4.0	4.0	4.0	4.0	29.0
36	8.78719512195122	4.0	4.0	4.0	4.0	30.0
37	8.520426829268292	4.0	4.0	4.0	4.0	29.0
38	8.359451219512195	4.0	4.0	4.0	4.0	28.0
39	8.231707317073171	4.0	4.0	4.0	4.0	28.0
40	8.173475609756098	4.0	4.0	4.0	4.0	28.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	1.3186157796451923
1	2	8.194549486461256
1	3	-3.548681139122319
1	4	7.55920868347339
1	5	8.752357609710558
1	6	5.760539215686272
1	7	10.106069094304395
1	8	3.619561157796454
1	9	0.4992180205415515
1	10	2.3294234360410826
1	11	-1.233123249299716
1	12	0.38370681605975676
1	13	-2.4927637721755396
1	14	-1.0703081232492977
1	15	4.242005135387494
1	16	-5.320179738562086
1	17	-2.3429621848739437
1	18	-3.1320261437908457
1	19	-4.303723155929042
1	20	-3.5156862745098003
1	21	-0.8403127917833864
1	22	-2.767238562091503
1	23	-1.519514472455647
1	24	-2.320985060690944
1	25	-1.3890289449112974
1	26	-0.7071195144724562
1	27	-0.6732026143790844
1	28	0.7560807656395898
1	29	-0.909115312791787
1	30	-3.2581349206349213
1	31	1.2314192343604091
1	32	-2.4217203548085884
1	33	0.671860410831
1	34	-1.971580298786181
1	35	-3.2639939309056967
1	36	-1.3330415499533146
1	37	-1.0260971055088692
1	38	-1.0813725490196084
1	39	-3.7482259570494865
1	40	-3.5912114845938374
5	1	-11.431384220354808
5	2	7.694549486461256
5	3	3.201318860877681
5	4	6.43420868347339
5	5	-3.2476423902894425
5	6	8.510539215686272
5	7	-1.6439309056956048
5	8	4.744561157796454
5	9	-3.7507819794584485
5	10	9.454423436041083
5	11	14.891876750700284
5	12	3.7587068160597568
5	13	2.2572362278244604
5	14	10.554691876750702
5	15	14.242005135387494
5	16	-5.195179738562086
5	17	-4.342962184873944
5	18	11.617973856209154
5	19	15.446276844070958
5	20	12.9843137254902
5	21	10.034687208216614
5	22	10.607761437908497
5	23	-2.019514472455647
5	24	0.4290149393090559
5	25	16.235971055088704
5	26	4.417880485527544
5	27	11.576797385620916
5	28	3.50608076563959
5	29	3.090884687208213
5	30	2.6168650793650787
5	31	14.48141923436041
5	32	19.20327964519141
5	33	-2.578139589169
5	34	15.653419701213819
5	35	11.611006069094303
5	36	8.791958450046685
5	37	24.098902894491133
5	38	-4.706372549019608
5	39	-3.7482259570494865
5	40	-3.5912114845938374
6	1	0.5686157796451923
6	2	5.944549486461256
6	3	6.701318860877681
6	4	2.43420868347339
6	5	8.002357609710558
6	6	5.010539215686272
6	7	4.356069094304395
6	8	-0.5054388422035458
6	9	7.2492180205415515
6	10	4.954423436041083
6	11	-0.35812324929971595
6	12	4.758706816059757
6	13	0.007236227824460428
6	14	2.3046918767507023
6	15	5.242005135387494
6	16	6.804820261437914
6	17	9.157037815126056
6	18	6.367973856209154
6	19	0.44627684407095813
6	20	7.2343137254902
6	21	14.784687208216614
6	22	6.357761437908497
6	23	9.230485527544353
6	24	9.429014939309056
6	25	14.735971055088703
6	26	19.667880485527544
6	27	15.576797385620916
6	28	15.50608076563959
6	29	22.090884687208213
6	30	14.116865079365079
6	31	7.981419234360409
6	32	2.2032796451914116
6	33	4.921860410831
6	34	10.153419701213819
6	35	11.861006069094303
6	36	8.791958450046685
6	37	9.59890289449113
6	38	6.043627450980392
6	39	10.751774042950514
6	40	10.408788515406162
7	1	-0.9313842203548077
7	2	7.944549486461256
7	3	1.7013188608776808
7	4	2.18420868347339
7	5	-0.7476423902894425
7	6	0.26053921568627203
7	7	3.606069094304395
7	8	3.244561157796454
7	9	6.2492180205415515
7	10	3.7044234360410826
7	11	-1.608123249299716
7	12	9.258706816059757
7	13	5.00723622782446
7	14	8.804691876750702
7	15	10.492005135387494
7	16	7.554820261437914
7	17	7.907037815126056
7	18	4.117973856209154
7	19	8.696276844070958
7	20	5.7343137254902
7	21	8.534687208216614
7	22	15.107761437908497
7	23	12.230485527544353
7	24	15.929014939309056
7	25	1.2359710550887026
7	26	-2.0821195144724562
7	27	0.5767973856209156
7	28	1.2560807656395898
7	29	-2.409115312791787
7	30	-3.3831349206349213
7	31	-2.768580765639591
7	32	-3.7967203548085884
7	33	-2.078139589169
7	34	-3.096580298786181
7	35	-0.8889939309056967
7	36	1.2919584500466854
7	37	1.3489028944911308
7	38	-0.20637254901960844
7	39	3.2517740429505135
7	40	3.6587885154061626
8	1	1.1686157796451937
8	2	2.094549486461254
8	3	-3.59868113912232
8	4	6.43420868347339
8	5	7.552357609710555
8	6	-2.489460784313728
8	7	-1.0439309056956034
8	8	-5.655438842203544
8	9	-2.35078197945845
8	10	2.4544234360410826
8	11	0.09187675070028334
8	12	-6.241293183940243
8	13	-8.14276377217554
8	14	-6.445308123249298
8	15	-12.957994864612505
8	16	-13.795179738562085
8	17	-10.942962184873943
8	18	-10.782026143790846
8	19	-9.753723155929041
8	20	-9.815686274509801
8	21	-8.765312791783387
8	22	-13.392238562091503
8	23	-13.019514472455647
8	24	-12.570985060690944
8	25	-10.764028944911297
8	26	-9.582119514472456
8	27	-9.423202614379084
8	28	-8.49391923436041
8	29	-7.909115312791787
8	30	-7.383134920634921
8	31	-7.518580765639591
8	32	-6.796720354808588
8	33	-5.578139589169
8	34	-5.346580298786181
8	35	-5.388993930905697
8	36	-5.208041549953315
8	37	-4.901097105508869
8	38	-4.706372549019608
8	39	-3.7482259570494865
8	40	-3.5912114845938374
9	1	1.1936157796451923
9	2	7.319549486461256
9	3	7.576318860877681
9	4	4.68420868347339
9	5	8.752357609710558
9	6	8.385539215686272
9	7	6.106069094304395
9	8	2.869561157796454
9	9	5.1242180205415515
9	10	11.954423436041083
9	11	1.266876750700284
9	12	1.6337068160597568
9	13	0.5072362278244604
9	14	5.429691876750702
9	15	6.117005135387494
9	16	3.8048202614379143
9	17	5.032037815126056
9	18	3.8679738562091543
9	19	2.571276844070958
9	20	5.7343137254902
9	21	8.409687208216614
9	22	4.357761437908497
9	23	7.230485527544353
9	24	9.929014939309056
9	25	13.360971055088703
9	26	8.292880485527544
9	27	13.576797385620916
9	28	12.38108076563959
9	29	11.715884687208213
9	30	9.116865079365079
9	31	7.981419234360409
9	32	5.703279645191412
9	33	5.046860410831
9	34	5.903419701213819
9	35	8.361006069094303
9	36	5.791958450046685
9	37	10.47390289449113
9	38	5.918627450980392
9	39	6.2517740429505135
9	40	6.783788515406163
10	1	-0.4313842203548077
10	2	-6.305450513538744
10	3	-15.79868113912232
10	4	-15.56579131652661
10	5	-0.24764239028944246
10	6	6.510539215686272
10	7	9.356069094304395
10	8	6.744561157796454
10	9	4.2492180205415515
10	10	5.454423436041083
10	11	13.891876750700284
10	12	13.758706816059757
10	13	15.25723622782446
10	14	15.554691876750702
10	15	16.242005135387494
10	16	11.804820261437914
10	17	10.657037815126056
10	18	1.6179738562091543
10	19	13.446276844070958
10	20	2.9843137254901997
10	21	-7.965312791783386
10	22	5.607761437908497
10	23	13.980485527544353
10	24	-12.570985060690944
10	25	-10.764028944911297
10	26	-9.582119514472456
10	27	-9.423202614379084
10	28	-8.49391923436041
10	29	-7.909115312791787
10	30	-7.383134920634921
10	31	-7.518580765639591
10	32	-6.796720354808588
10	33	-5.578139589169
10	34	-5.346580298786181
10	35	-5.388993930905697
10	36	-5.208041549953315
10	37	-4.901097105508869
10	38	-4.706372549019608
10	39	-3.7482259570494865
10	40	-3.5912114845938374
11	1	2.997187208216623
11	2	3.408835200746971
11	3	2.344176003734823
11	4	4.577065826330536
11	5	1.0380718954248422
11	6	2.939110644257699
11	7	1.2132119514472528
11	8	2.601704014939312
11	9	-1.8936391223155908
11	10	3.3115662931839402
11	11	2.320448179271711
11	12	2.472992530345472
11	13	3.4000933706816028
11	14	2.6975490196078447
11	15	9.242005135387494
11	16	5.947677404295057
11	17	5.657037815126056
11	18	4.475116713352012
11	19	2.446276844070958
11	20	1.9843137254901997
11	21	5.320401493930898
11	22	3.607761437908497
11	23	-0.59094304388422
11	24	-0.2852707749766594
11	25	3.8073996265172756
11	26	3.132166199813259
11	27	3.0053688141923427
11	28	5.50608076563959
11	29	3.090884687208213
11	30	1.759722222222221
11	31	-2.6614379084967332
11	32	4.203279645191412
11	33	4.1361461251167135
11	34	1.653419701213819
11	35	1.8967203548085898
11	36	0.22052987861811424
11	37	-1.6153828197945836
11	38	2.2936274509803916
11	39	2.394631185807656
11	40	3.9802170868347337
12	1	-3.4313842203548077
12	2	4.294549486461257
12	3	3.201318860877681
12	4	4.634208683473393
12	5	2.152357609710556
12	6	3.510539215686272
12	7	-0.8439309056956041
12	8	0.9445611577964534
12	9	-1.7507819794584485
12	10	6.254423436041083
12	11	7.891876750700284
12	12	4.5587068160597575
12	13	5.057236227824461
12	14	4.154691876750704
12	15	9.642005135387492
12	16	10.804820261437914
12	17	6.257037815126058
12	18	3.6179738562091543
12	19	14.04627684407096
12	20	7.9843137254902
12	21	9.834687208216614
12	22	7.807761437908496
12	23	9.180485527544352
12	24	8.629014939309055
12	25	4.835971055088704
12	26	9.217880485527544
12	27	11.976797385620914
12	28	6.706080765639589
12	29	10.090884687208213
12	30	10.41686507936508
12	31	4.681419234360408
12	32	4.003279645191412
12	33	-3.378139589169
12	34	-0.9465802987861807
12	35	0.21100606909430297
12	36	-3.0080415499533144
12	37	-2.701097105508869
12	38	-2.5063725490196083
12	39	1.8517740429505132
12	40	-1.3912114845938373
13	1	3.1400443510737617
13	2	6.55169234360411
13	3	1.7727474323062538
13	4	6.7199229691876745
13	5	8.323786181139127
13	6	0.510539215686272
13	7	1.3560690943043952
13	8	3.315989729225027
13	9	4.392075163398694
13	10	7.31156629318394
13	11	5.463305322128857
13	12	9.901563958916899
13	13	6.542950513538745
13	14	7.840406162464987
13	15	6.099147992530352
13	16	13.804820261437914
13	17	8.657037815126056
13	18	6.046545284780581
13	19	6.446276844070958
13	20	5.9843137254902
13	21	7.891830065359471
13	22	4.750618580765639
13	23	5.123342670401495
13	24	7.429014939309056
13	25	6.950256769374418
13	26	4.132166199813259
13	27	0.719654528478058
13	28	2.2203664799253033
13	29	5.376598972922498
13	30	3.18829365079365
13	31	4.6242763772175515
13	32	6.060422502334269
13	33	6.993288982259573
13	34	3.7962768440709613
13	35	4.611006069094303
13	36	2.7919584500466854
13	37	-0.043954248366011583
13	38	1.7221988795518204
13	39	3.394631185807656
13	40	5.26593137254902
15	1	3.7352824463118566
15	2	8.86121615312792
15	3	3.034652194211013
15	4	10.43420868347339
15	5	8.752357609710558
15	6	8.343872549019608
15	7	9.356069094304395
15	8	6.9112278244631185
15	9	8.249218020541552
15	10	10.12109010270775
15	11	12.22521008403362
15	12	5.425373482726425
15	13	11.75723622782446
15	14	8.22135854341737
15	15	9.075338468720826
15	16	6.6381535947712464
15	17	9.323704481792724
15	18	9.284640522875822
15	19	2.2796101774042903
15	20	7.817647058823532
15	21	11.701353874883281
15	22	5.441094771241829
15	23	10.14715219421102
15	24	13.762348272642388
15	25	9.235971055088703
15	26	6.417880485527544
15	27	8.243464052287583
15	28	5.839414098972922
15	29	3.090884687208213
15	30	5.783531746031747
15	31	4.981419234360409
15	32	3.8699463118580777
15	33	4.421860410831
15	34	4.986753034547153
15	35	7.777672735760971
15	36	0.9586251167133515
15	37	4.598902894491131
15	38	4.293627450980392
15	39	2.9184407096171796
15	40	2.5754551820728286
16	1	-0.631384220354807
16	2	-1.1054505135387451
16	3	-0.5986811391223199
16	4	-4.7657913165266095
16	5	-0.8476423902894439
16	6	0.510539215686272
16	7	-3.2439309056956063
16	8	-4.855438842203547
16	9	0.2492180205415515
16	10	3.854423436041081
16	11	2.6918767507002848
16	12	1.958706816059756
16	13	2.4572362278244597
16	14	2.954691876750701
16	15	0.6420051353874925
16	16	9.404820261437916
16	17	12.257037815126058
16	18	5.617973856209154
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109	11	-1.108123249299716
109	12	-1.9912931839402432
109	13	-5.49276377217554
109	14	-5.695308123249298
109	15	-2.757994864612506
109	16	3.0548202614379143
109	17	-4.092962184873944
109	18	-1.8820261437908457
109	19	-11.803723155929042
109	20	-5.2656862745098
109	21	-3.9653127917833864
109	22	-2.392238562091503
109	23	-1.769514472455647
109	24	-1.3209850606909441
109	25	-4.514028944911297
109	26	-3.3321195144724562
109	27	1.5767973856209156
109	28	0.7560807656395898
109	29	-3.659115312791787
109	30	-1.8831349206349213
109	31	-0.2685807656395909
109	32	0.4532796451914116
109	33	-5.578139589169
109	34	-5.346580298786181
109	35	-5.388993930905697
109	36	-5.208041549953315
109	37	-4.901097105508869
109	38	-4.706372549019608
109	39	-3.7482259570494865
109	40	-3.5912114845938374
110	1	-1.6813842203548077
110	2	5.194549486461256
110	3	3.201318860877681
110	4	6.68420868347339
110	5	-9.497642390289442
110	6	0.26053921568627203
110	7	-6.143930905695605
110	8	5.244561157796454
110	9	3.2492180205415515
110	10	6.454423436041083
110	11	-3.108123249299716
110	12	1.7587068160597568
110	13	0.5072362278244604
110	14	4.554691876750702
110	15	-5.007994864612506
110	16	-1.4451797385620857
110	17	-2.5929621848739437
110	18	-7.882026143790846
110	19	-11.303723155929042
110	20	-1.2656862745098003
110	21	-10.715312791783386
110	22	-7.392238562091503
110	23	-6.269514472455647
110	24	-6.070985060690944
110	25	-10.764028944911297
110	26	-9.582119514472456
110	27	-9.423202614379084
110	28	-8.49391923436041
110	29	-7.909115312791787
110	30	-7.383134920634921
110	31	-7.518580765639591
110	32	-6.796720354808588
110	33	-5.578139589169
110	34	-5.346580298786181
110	35	-5.388993930905697
110	36	-5.208041549953315
110	37	-4.901097105508869
110	38	-4.706372549019608
110	39	-3.7482259570494865
110	40	-3.5912114845938374
111	1	0.5686157796451923
111	2	-1.3054505135387444
111	3	4.701318860877681
111	4	6.93420868347339
111	5	0.00235760971055754
111	6	3.260539215686272
111	7	-0.14393090569560485
111	8	2.744561157796454
111	9	-5.0007819794584485
111	10	-7.545576563958917
111	11	0.891876750700284
111	12	-1.4912931839402432
111	13	-1.9927637721755396
111	14	-3.9453081232492977
111	15	-6.507994864612506
111	16	-3.1951797385620857
111	17	-0.0929621848739437
111	18	-3.6320261437908457
111	19	-0.053723155929041866
111	20	-4.5156862745098
111	21	-3.2153127917833864
111	22	1.107761437908497
111	23	-2.269514472455647
111	24	0.9290149393090559
111	25	-1.7640289449112974
111	26	4.167880485527544
111	27	-7.923202614379084
111	28	-1.7439192343604102
111	29	-7.909115312791787
111	30	-7.383134920634921
111	31	-7.518580765639591
111	32	-6.796720354808588
111	33	-5.578139589169
111	34	-5.346580298786181
111	35	-5.388993930905697
111	36	-5.208041549953315
111	37	-4.901097105508869
111	38	-4.706372549019608
111	39	-3.7482259570494865
111	40	-3.5912114845938374
112	1	2.5686157796451923
112	2	6.194549486461256
112	3	0.7013188608776808
112	4	-3.56579131652661
112	5	-0.7476423902894425
112	6	3.010539215686272
112	7	-9.143930905695605
112	8	6.744561157796454
112	9	-19.75078197945845
112	10	-17.545576563958917
112	11	-17.108123249299716
112	12	-17.241293183940243
112	13	-18.74276377217554
112	14	-18.445308123249298
112	15	-17.757994864612506
112	16	-16.195179738562086
112	17	-15.342962184873944
112	18	-16.382026143790846
112	19	-14.553723155929042
112	20	-15.0156862745098
112	21	-13.965312791783386
112	22	-13.392238562091503
112	23	-13.019514472455647
112	24	-12.570985060690944
112	25	-10.764028944911297
112	26	-9.582119514472456
112	27	-9.423202614379084
112	28	-8.49391923436041
112	29	-7.909115312791787
112	30	-7.383134920634921
112	31	-7.518580765639591
112	32	-6.796720354808588
112	33	-5.578139589169
112	34	-5.346580298786181
112	35	-5.388993930905697
112	36	-5.208041549953315
112	37	-4.901097105508869
112	38	-4.706372549019608
112	39	-3.7482259570494865
112	40	-3.5912114845938374
113	1	-3.0980508870214756
113	2	5.694549486461256
113	3	5.5346521942110165
113	4	8.100875350140054
113	5	10.419024276377222
113	6	5.17720588235294
113	7	-7.977264239028939
113	8	1.0778944911297863
113	9	-6.7507819794584485
113	10	-1.8789098972922496
113	11	4.891876750700284
113	12	-0.24129318394024324
113	13	-4.409430438842207
113	14	0.5546918767507023
113	15	-4.757994864612506
113	16	1.8048202614379143
113	17	10.657037815126056
113	18	1.9513071895424865
113	19	6.112943510737626
113	20	9.317647058823532
113	21	-0.2986461251167185
113	22	3.941094771241829
113	23	-7.019514472455647
113	24	-9.570985060690944
113	25	-8.430695611577963
113	26	-2.2487861811391223
113	27	-4.08986928104575
113	28	-5.827252567693743
113	29	-2.909115312791787
113	30	-1.0498015873015873
113	31	1.4814192343604091
113	32	-2.4633870214752545
113	33	-1.244806255835666
113	34	4.986753034547153
113	35	-1.0556605975723627
113	36	-0.8747082166199807
113	37	-0.5677637721755353
113	38	-0.3730392156862745
113	39	0.5851073762838475
113	40	5.7421218487394965
116	1	-1.4313842203548077
116	2	-0.3054505135387444
116	3	0.2013188608776808
116	4	4.93420868347339
116	5	-2.7476423902894425
116	6	6.510539215686272
116	7	9.356069094304395
116	8	7.744561157796454
116	9	3.7492180205415515
116	10	6.454423436041083
116	11	5.391876750700284
116	12	5.758706816059757
116	13	3.7572362278244604
116	14	5.054691876750702
116	15	3.742005135387494
116	16	5.804820261437914
116	17	12.657037815126056
116	18	10.117973856209154
116	19	12.446276844070958
116	20	3.4843137254901997
116	21	4.534687208216614
116	22	14.607761437908497
116	23	12.480485527544353
116	24	0.9290149393090559
116	25	3.7359710550887026
116	26	2.9178804855275438
116	27	3.0767973856209156
116	28	0.5060807656395898
116	29	-0.40911531279178703
116	30	8.116865079365079
116	31	5.981419234360409
116	32	-6.796720354808588
116	33	-5.578139589169
116	34	-5.346580298786181
116	35	-5.388993930905697
116	36	-5.208041549953315
116	37	-4.901097105508869
116	38	-4.706372549019608
116	39	-3.7482259570494865
116	40	-3.5912114845938374
117	1	-2.4313842203548077
117	2	-2.3054505135387444
117	3	-2.798681139122319
117	4	0.4342086834733898
117	5	-12.247642390289442
117	6	8.510539215686272
117	7	-4.643930905695605
117	8	-11.255438842203546
117	9	9.249218020541552
117	10	4.454423436041083
117	11	-4.108123249299716
117	12	6.758706816059757
117	13	12.25723622782446
117	14	-18.445308123249298
117	15	-17.757994864612506
117	16	-16.195179738562086
117	17	-15.342962184873944
117	18	-16.382026143790846
117	19	-14.553723155929042
117	20	-15.0156862745098
117	21	-13.965312791783386
117	22	-13.392238562091503
117	23	-13.019514472455647
117	24	-12.570985060690944
117	25	-10.764028944911297
117	26	-9.582119514472456
117	27	-9.423202614379084
117	28	-8.49391923436041
117	29	-7.909115312791787
117	30	-7.383134920634921
117	31	-7.518580765639591
117	32	-6.796720354808588
117	33	-5.578139589169
117	34	-5.346580298786181
117	35	-5.388993930905697
117	36	-5.208041549953315
117	37	-4.901097105508869
117	38	-4.706372549019608
117	39	-3.7482259570494865
117	40	-3.5912114845938374
119	1	4.568615779645192
119	2	5.694549486461256
119	3	8.20131886087768
119	4	2.43420868347339
119	5	6.7523576097105575
119	6	-3.489460784313728
119	7	6.356069094304395
119	8	3.744561157796454
119	9	6.2492180205415515
119	10	-12.545576563958917
119	11	9.891876750700284
119	12	3.7587068160597568
119	13	7.25723622782446
119	14	13.554691876750702
119	15	-11.757994864612506
119	16	4.804820261437914
119	17	10.657037815126056
119	18	15.617973856209154
119	19	9.446276844070958
119	20	10.9843137254902
119	21	-3.9653127917833864
119	22	7.607761437908497
119	23	14.980485527544353
119	24	17.429014939309056
119	25	19.235971055088704
119	26	12.417880485527544
119	27	12.576797385620916
119	28	22.506080765639588
119	29	-7.909115312791787
119	30	-7.383134920634921
119	31	-7.518580765639591
119	32	-6.796720354808588
119	33	-5.578139589169
119	34	-5.346580298786181
119	35	-5.388993930905697
119	36	-5.208041549953315
119	37	-4.901097105508869
119	38	-4.706372549019608
119	39	-3.7482259570494865
119	40	-3.5912114845938374
120	1	6.568615779645192
120	2	4.194549486461256
120	3	6.201318860877681
120	4	7.93420868347339
120	5	8.252357609710558
120	6	8.510539215686272
120	7	-14.643930905695605
120	8	3.744561157796454
120	9	-0.7507819794584485
120	10	-1.0455765639589174
120	11	4.391876750700284
120	12	-7.241293183940243
120	13	5.25723622782446
120	14	10.554691876750702
120	15	11.242005135387494
120	16	-4.695179738562086
120	17	-3.8429621848739437
120	18	9.117973856209154
120	19	-4.053723155929042
120	20	0.4843137254901997
120	21	-13.965312791783386
120	22	-13.392238562091503
120	23	-13.019514472455647
120	24	-12.570985060690944
120	25	-10.764028944911297
120	26	-9.582119514472456
120	27	-9.423202614379084
120	28	-8.49391923436041
120	29	-7.909115312791787
120	30	-7.383134920634921
120	31	-7.518580765639591
120	32	-6.796720354808588
120	33	-5.578139589169
120	34	-5.346580298786181
120	35	-5.388993930905697
120	36	-5.208041549953315
120	37	-4.901097105508869
120	38	-4.706372549019608
120	39	-3.7482259570494865
120	40	-3.5912114845938374
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	246.0
5	74.0
6	64.0
7	74.0
8	63.0
9	81.0
10	86.0
11	93.0
12	110.0
13	113.0
14	132.0
15	158.0
16	138.0
17	150.0
18	134.0
19	170.0
20	181.0
21	185.0
22	170.0
23	167.0
24	131.0
25	128.0
26	113.0
27	108.0
28	74.0
29	50.0
30	40.0
31	24.0
32	13.0
33	5.0
34	3.0
35	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.01647848642051	1.2511443393347574	0.45773573390296	0.274641440341776
8	96.67277167277167	1.6483516483516485	0.5494505494505495	1.1294261294261294
9	91.48871262965223	5.491153142159853	1.7083587553386212	1.3117754728492983
10	41.3109756097561	34.512195121951216	10.640243902439025	13.536585365853659
11	34.573170731707314	23.932926829268293	23.597560975609756	17.896341463414632
12	34.827691369319915	17.47483989021043	23.452272034156753	24.2451967063129
13	24.76364745349192	19.82311680390363	29.094236047575478	26.31899969502897
14	19.878048780487806	25.396341463414636	30.853658536585364	23.871951219512194
15	26.432926829268293	23.96341463414634	28.536585365853657	21.067073170731707
16	30.100640439158283	23.025312595303447	25.282098200670937	21.59194876486734
17	23.6890243902439	24.359756097560975	27.31707317073171	24.634146341463413
18	29.39024390243902	22.926829268292686	22.25609756097561	25.426829268292682
19	20.293398533007334	18.09290953545232	20.568459657701712	41.045232273838636
20	25.823170731707318	26.890243902439025	24.085365853658537	23.201219512195124
21	24.634146341463413	21.76829268292683	29.268292682926827	24.329268292682926
22	21.676829268292682	19.817073170731707	28.96341463414634	29.542682926829265
23	25.48780487804878	20.243902439024392	29.45121951219512	24.817073170731707
24	24.09271119243672	22.964318389752975	27.87435193656603	25.068618481244282
25	21.805428484293994	24.00121988411101	27.477889600487952	26.715462031107045
26	19.173047473200615	22.6646248085758	32.52679938744257	25.63552833078101
27	19.548642878926504	18.84720951509607	32.265934736200066	29.33821286977737
28	18.37046078730546	20.96429661275557	31.94995422642661	28.715288373512358
29	21.210268948655255	18.09290953545232	32.36552567237164	28.33129584352078
30	18.828196521208422	16.47848642050656	37.4122673176686	27.28104974061642
31	22.137404580152673	18.076335877862597	36.36641221374045	23.419847328244277
32	21.361000915471468	15.685077815074763	38.26670735428746	24.68721391516631
33	20.59182428309945	16.96156192800488	40.26845637583892	22.17815741305674
34	15.701219512195122	17.103658536585368	44.146341463414636	23.04878048780488
35	14.704087858450274	17.876754118364858	44.56985967053081	22.849298352654056
36	15.436241610738255	19.70713849908481	40.543014032946914	24.31360585723002
37	16.49358582773366	18.356750152718387	40.042761148442274	25.106902871105678
38	16.4174549893195	19.13335367714373	40.73848031736344	23.71071101617333
39	18.51738865161684	17.38865161683954	39.9633923123856	24.130567419158023
40	15.827996340347667	18.420250076242755	41.84202500762428	23.909728575785298
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	2.0
14	2.5
15	3.0
16	6.0
17	9.0
18	9.0
19	11.0
20	13.0
21	21.5
22	30.0
23	30.0
24	40.5
25	51.0
26	48.5
27	46.0
28	46.0
29	60.5
30	75.0
31	75.5
32	76.0
33	76.0
34	88.0
35	100.0
36	120.5
37	141.0
38	141.0
39	162.0
40	183.0
41	196.0
42	209.0
43	209.0
44	207.0
45	205.0
46	207.0
47	209.0
48	209.0
49	206.5
50	204.0
51	200.0
52	196.0
53	196.0
54	208.5
55	221.0
56	228.0
57	235.0
58	235.0
59	224.0
60	213.0
61	220.5
62	228.0
63	228.0
64	232.0
65	236.0
66	200.5
67	165.0
68	165.0
69	129.0
70	93.0
71	78.0
72	63.0
73	63.0
74	54.0
75	45.0
76	29.5
77	14.0
78	14.0
79	13.0
80	12.0
81	7.0
82	2.0
83	2.0
84	1.5
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.09146341463414635
8	0.12195121951219512
9	0.06097560975609756
10	0.0
11	0.0
12	0.03048780487804878
13	0.03048780487804878
14	0.0
15	0.0
16	0.03048780487804878
17	0.0
18	0.0
19	0.24390243902439024
20	0.0
21	0.0
22	0.0
23	0.0
24	0.03048780487804878
25	0.03048780487804878
26	0.45731707317073167
27	0.03048780487804878
28	0.09146341463414635
29	0.24390243902439024
30	0.09146341463414635
31	0.1524390243902439
32	0.09146341463414635
33	0.06097560975609756
34	0.0
35	0.06097560975609756
36	0.06097560975609756
37	0.1829268292682927
38	0.09146341463414635
39	0.06097560975609756
40	0.03048780487804878
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
40	3280.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.60365853658537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.87495971640348	90.70121951219512
2	3.4160489848533677	6.463414634146342
3	0.41894940380277146	1.1890243902439024
4	0.16113438607798905	0.6097560975609756
5	0.03222687721559781	0.1524390243902439
6	0.03222687721559781	0.1829268292682927
7	0.03222687721559781	0.2134146341463415
8	0.0	0.0
9	0.0	0.0
>10	0.03222687721559781	0.4878048780487805
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGC	16	0.4878048780487805	No Hit
AGATGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTT	7	0.2134146341463415	No Hit
AGATGGGGGATGTCATTCTGTGCATCTGGGGTTATCTGGG	6	0.1829268292682927	No Hit
AGATGGGGGGGGCTGGTGCGATGGCTCAGTGGGTAAGAGC	5	0.1524390243902439	No Hit
AGATGGGGGATTAGTGATGATGTGATTGTCGGCCGGTGCC	4	0.12195121951219512	No Hit
AGATGGGGGGTGTAGAACTGCTCCAGGCTTTCTTCCTTGT	4	0.12195121951219512	No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCCGTGGGTACGAGC	4	0.12195121951219512	No Hit
AGATGGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.12195121951219512	No Hit
AGATGGGGGGGGCTGGTGAGATGGCTCCGTGGGTAAGAGC	4	0.12195121951219512	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.03048780487804878	0.0	0.0	0.0	0.0
14	0.06097560975609756	0.0	0.0	0.0	0.0
15	0.06097560975609756	0.0	0.0	0.0	0.0
16	0.06097560975609756	0.0	0.0	0.0	0.0
17	0.06097560975609756	0.0	0.0	0.0	0.0
18	0.06097560975609756	0.0	0.0	0.0	0.0
19	0.06097560975609756	0.0	0.0	0.0	0.0
20	0.06097560975609756	0.0	0.0	0.0	0.0
21	0.06097560975609756	0.0	0.0	0.0	0.0
22	0.06097560975609756	0.0	0.0	0.0	0.0
23	0.06097560975609756	0.0	0.0	0.0	0.0
24	0.06097560975609756	0.0	0.0	0.0	0.0
25	0.06097560975609756	0.0	0.0	0.0	0.0
26	0.06097560975609756	0.0	0.0	0.0	0.0
27	0.06097560975609756	0.0	0.0	0.0	0.0
28	0.06097560975609756	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATGGCT	25	1.4140629E-4	34.746033	20
TGGCTCA	20	0.002148013	34.746033	22
GGGGGAT	35	7.689705E-7	33.676926	5
GGGGGAG	35	7.689705E-7	33.676926	5
ATGGGGG	260	0.0	33.67692	3
ATGGGGA	50	2.1100277E-10	33.67692	3
TGGGGGT	40	5.0660674E-8	33.67692	4
TGGGGGG	75	0.0	33.67692	4
TGGGGGA	130	0.0	33.67692	4
GGGGCTG	20	0.0025165568	33.67692	9
GGGCTGG	25	1.7123159E-4	33.67692	10
AGATGGG	325	0.0	33.158817	1
GATGGGG	315	0.0	33.14237	2
ATGGCTC	30	4.1292087E-4	28.955027	21
GGGGGGT	30	4.996236E-4	28.064102	5
GGGGGGC	30	4.996236E-4	28.064102	7
TGGGGAG	25	0.0075066965	26.941538	4
GGGGGCT	25	0.0075066965	26.941538	8
GGGGGAC	25	0.0075066965	26.941538	5
GGGGGAA	50	3.6043275E-7	26.941538	5
>>END_MODULE